pH and redox sensitive albumin hydrogel: A self-derived biomaterial
Serum albumin can be transformed to a stimuli (pH and redox) responsive hydrogel using the reduction process followed by oxidative refolding. The preparation of albumin hydrogel involves a range of concentrations (75, 150, 300, 450, 600 and 750 μM) and pH (2.0–10.0) values and the gelation begins at...
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| description | Serum albumin can be transformed to a stimuli (pH and redox) responsive hydrogel using the reduction process followed by oxidative refolding. The preparation of albumin hydrogel involves a range of concentrations (75, 150, 300, 450, 600 and 750 μM) and pH (2.0–10.0) values and the gelation begins at a concentration of 150 μM and 4.5–8.0 pH value. The hydrogel shows maximum swelling at alkali pH (pH > 9.0). The increase in albumin concentration increases hydrogel stability, rheological property, compressive strength, proteolytic resistance and rate of
in vivo
biodegradation. Based on the observed physical and biological properties of albumin hydrogel, 450 μM was determined to be an optimum concentration for further experiments. In addition, the hemo- and cytocompatibility analyses revealed the biocompatibility nature of albumin hydrogel. The experiments on
in vitro
drug (Tetracycline) delivery were carried out under non reducing and reducing conditions that resulted in the sustained and fast release of the drug, respectively. The methodology used in the preparation of albumin hydrogel may lead to the development of autogenic tissue constructs. In addition, the methodology can have various applications in tissue engineering and drug delivery. |
| doi_str_mv | 10.1038/srep15977 |
| format | Article |
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in vivo
biodegradation. Based on the observed physical and biological properties of albumin hydrogel, 450 μM was determined to be an optimum concentration for further experiments. In addition, the hemo- and cytocompatibility analyses revealed the biocompatibility nature of albumin hydrogel. The experiments on
in vitro
drug (Tetracycline) delivery were carried out under non reducing and reducing conditions that resulted in the sustained and fast release of the drug, respectively. The methodology used in the preparation of albumin hydrogel may lead to the development of autogenic tissue constructs. In addition, the methodology can have various applications in tissue engineering and drug delivery.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep15977</identifier><identifier>PMID: 26527296</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/45 ; 631/61 ; Albumin ; Animals ; Biocompatibility ; Biocompatible Materials - chemistry ; Biocompatible Materials - pharmacology ; Biodegradation ; Cattle ; Cell Survival - drug effects ; Circular Dichroism ; Drug Carriers - chemistry ; Drug delivery ; Drug Delivery Systems - methods ; Erythrocytes - cytology ; Erythrocytes - drug effects ; Erythrocytes - metabolism ; Female ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Gelation ; Humanities and Social Sciences ; Humans ; Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry ; Hydrogels ; Hydrogen-Ion Concentration ; Mice ; Microscopy, Electron, Scanning ; multidisciplinary ; NIH 3T3 Cells ; Oxidation-Reduction ; pH effects ; Proteolysis ; Rats, Wistar ; Science ; Serum Albumin - chemistry ; Serum Albumin, Bovine - chemistry ; Tetracycline - administration & dosage ; Tetracycline - pharmacokinetics ; Tissue engineering</subject><ispartof>Scientific reports, 2015-11, Vol.5 (1), p.15977, Article 15977</ispartof><rights>The Author(s) 2015</rights><rights>Copyright Nature Publishing Group Nov 2015</rights><rights>Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-a11c1ef603cbdd63840d9a22d771f2779847e43f3412addf6cd85d884506d9553</citedby><cites>FETCH-LOGICAL-c438t-a11c1ef603cbdd63840d9a22d771f2779847e43f3412addf6cd85d884506d9553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630586/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4630586/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,41101,42170,51557,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26527296$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Raja, S Thirupathi Kumara</creatorcontrib><creatorcontrib>Thiruselvi, T</creatorcontrib><creatorcontrib>Mandal, Asit Baran</creatorcontrib><creatorcontrib>Gnanamani, A</creatorcontrib><title>pH and redox sensitive albumin hydrogel: A self-derived biomaterial</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Serum albumin can be transformed to a stimuli (pH and redox) responsive hydrogel using the reduction process followed by oxidative refolding. The preparation of albumin hydrogel involves a range of concentrations (75, 150, 300, 450, 600 and 750 μM) and pH (2.0–10.0) values and the gelation begins at a concentration of 150 μM and 4.5–8.0 pH value. The hydrogel shows maximum swelling at alkali pH (pH > 9.0). The increase in albumin concentration increases hydrogel stability, rheological property, compressive strength, proteolytic resistance and rate of
in vivo
biodegradation. Based on the observed physical and biological properties of albumin hydrogel, 450 μM was determined to be an optimum concentration for further experiments. In addition, the hemo- and cytocompatibility analyses revealed the biocompatibility nature of albumin hydrogel. The experiments on
in vitro
drug (Tetracycline) delivery were carried out under non reducing and reducing conditions that resulted in the sustained and fast release of the drug, respectively. The methodology used in the preparation of albumin hydrogel may lead to the development of autogenic tissue constructs. In addition, the methodology can have various applications in tissue engineering and drug delivery.</description><subject>631/45</subject><subject>631/61</subject><subject>Albumin</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Biodegradation</subject><subject>Cattle</subject><subject>Cell Survival - drug effects</subject><subject>Circular Dichroism</subject><subject>Drug Carriers - chemistry</subject><subject>Drug delivery</subject><subject>Drug Delivery Systems - methods</subject><subject>Erythrocytes - cytology</subject><subject>Erythrocytes - drug effects</subject><subject>Erythrocytes - metabolism</subject><subject>Female</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Gelation</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry</subject><subject>Hydrogels</subject><subject>Hydrogen-Ion Concentration</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning</subject><subject>multidisciplinary</subject><subject>NIH 3T3 Cells</subject><subject>Oxidation-Reduction</subject><subject>pH effects</subject><subject>Proteolysis</subject><subject>Rats, Wistar</subject><subject>Science</subject><subject>Serum Albumin - chemistry</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Tetracycline - administration & dosage</subject><subject>Tetracycline - pharmacokinetics</subject><subject>Tissue engineering</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkMtKA0EQRRtRjMQs_AEZcKUw2u-HCyEENULAja6bnumeZMK87J4E8_d2SAwRa1NV3MOt4gJwheA9gkQ-BO86xJQQJ-ACQ8pSTDA-PZoHYBTCEsZiWFGkzsEAc4YFVvwCTLppYhqbeGfb7yS4JpR9uXaJqbJVXTbJYmN9O3fVYzKOalWk1vmo2yQr29r0cTHVJTgrTBXcaN-H4PPl-WMyTWfvr2-T8SzNKZF9ahDKkSs4JHlmLSeSQqsMxlYIVGAhlKTCUVIQirCxtuC5lcxKSRnkVjFGhuBp59utstrZ3DW9N5XufFkbv9GtKfVfpSkXet6uNeUEMsmjwc3ewLdfKxd6vWxXvok_aySVklAisaVud1Tu2xDTLQ4XENTbyPUh8sheH790IH8DjsDdDghRaubOH5385_YDznWKuA</recordid><startdate>20151103</startdate><enddate>20151103</enddate><creator>Raja, S Thirupathi Kumara</creator><creator>Thiruselvi, T</creator><creator>Mandal, Asit Baran</creator><creator>Gnanamani, A</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20151103</creationdate><title>pH and redox sensitive albumin hydrogel: A self-derived biomaterial</title><author>Raja, S Thirupathi Kumara ; Thiruselvi, T ; Mandal, Asit Baran ; Gnanamani, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-a11c1ef603cbdd63840d9a22d771f2779847e43f3412addf6cd85d884506d9553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>631/45</topic><topic>631/61</topic><topic>Albumin</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Biodegradation</topic><topic>Cattle</topic><topic>Cell Survival - drug effects</topic><topic>Circular Dichroism</topic><topic>Drug Carriers - chemistry</topic><topic>Drug delivery</topic><topic>Drug Delivery Systems - methods</topic><topic>Erythrocytes - cytology</topic><topic>Erythrocytes - drug effects</topic><topic>Erythrocytes - metabolism</topic><topic>Female</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Gelation</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry</topic><topic>Hydrogels</topic><topic>Hydrogen-Ion Concentration</topic><topic>Mice</topic><topic>Microscopy, Electron, Scanning</topic><topic>multidisciplinary</topic><topic>NIH 3T3 Cells</topic><topic>Oxidation-Reduction</topic><topic>pH effects</topic><topic>Proteolysis</topic><topic>Rats, Wistar</topic><topic>Science</topic><topic>Serum Albumin - chemistry</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Tetracycline - administration & dosage</topic><topic>Tetracycline - pharmacokinetics</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raja, S Thirupathi Kumara</creatorcontrib><creatorcontrib>Thiruselvi, T</creatorcontrib><creatorcontrib>Mandal, Asit Baran</creatorcontrib><creatorcontrib>Gnanamani, A</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raja, S Thirupathi Kumara</au><au>Thiruselvi, T</au><au>Mandal, Asit Baran</au><au>Gnanamani, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>pH and redox sensitive albumin hydrogel: A self-derived biomaterial</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2015-11-03</date><risdate>2015</risdate><volume>5</volume><issue>1</issue><spage>15977</spage><pages>15977-</pages><artnum>15977</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Serum albumin can be transformed to a stimuli (pH and redox) responsive hydrogel using the reduction process followed by oxidative refolding. The preparation of albumin hydrogel involves a range of concentrations (75, 150, 300, 450, 600 and 750 μM) and pH (2.0–10.0) values and the gelation begins at a concentration of 150 μM and 4.5–8.0 pH value. The hydrogel shows maximum swelling at alkali pH (pH > 9.0). The increase in albumin concentration increases hydrogel stability, rheological property, compressive strength, proteolytic resistance and rate of
in vivo
biodegradation. Based on the observed physical and biological properties of albumin hydrogel, 450 μM was determined to be an optimum concentration for further experiments. In addition, the hemo- and cytocompatibility analyses revealed the biocompatibility nature of albumin hydrogel. The experiments on
in vitro
drug (Tetracycline) delivery were carried out under non reducing and reducing conditions that resulted in the sustained and fast release of the drug, respectively. The methodology used in the preparation of albumin hydrogel may lead to the development of autogenic tissue constructs. In addition, the methodology can have various applications in tissue engineering and drug delivery.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26527296</pmid><doi>10.1038/srep15977</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 631/45 631/61 Albumin Animals Biocompatibility Biocompatible Materials - chemistry Biocompatible Materials - pharmacology Biodegradation Cattle Cell Survival - drug effects Circular Dichroism Drug Carriers - chemistry Drug delivery Drug Delivery Systems - methods Erythrocytes - cytology Erythrocytes - drug effects Erythrocytes - metabolism Female Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - metabolism Gelation Humanities and Social Sciences Humans Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry Hydrogels Hydrogen-Ion Concentration Mice Microscopy, Electron, Scanning multidisciplinary NIH 3T3 Cells Oxidation-Reduction pH effects Proteolysis Rats, Wistar Science Serum Albumin - chemistry Serum Albumin, Bovine - chemistry Tetracycline - administration & dosage Tetracycline - pharmacokinetics Tissue engineering |
| title | pH and redox sensitive albumin hydrogel: A self-derived biomaterial |
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