Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism
Huangqi Sanxian decoction (HQSXD) is routinely used for the treatment of osteoporosis in the Chinese traditional healthcare system. However, the targets and mechanism underlying the effect of HQSXD on osteoporosis have not been documented. In the present study, seropharmacology and proteomic approac...
Gespeichert in:
| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2015-01, Vol.2015 (2015), p.1-10 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 10 |
|---|---|
| container_issue | 2015 |
| container_start_page | 1 |
| container_title | Evidence-based complementary and alternative medicine |
| container_volume | 2015 |
| creator | Zhou, Zhi-Kun Zhou, Yan-Xing Zhu, Jian-Hong Fu, Jian-Ying Zheng, Li-Hua Guo, Chong-Chong Zeng, Tao |
| description | Huangqi Sanxian decoction (HQSXD) is routinely used for the treatment of osteoporosis in the Chinese traditional healthcare system. However, the targets and mechanism underlying the effect of HQSXD on osteoporosis have not been documented. In the present study, seropharmacology and proteomic approaches (two-dimensional gel electrophoresis combined with mass spectrometry) were used to investigate the effects and possible target proteins of HQSXD on osteoblast. We found that HQSXD-treated rat serum significantly enhanced osteoblast proliferation, differentiation, and mineralization. In HQSXD-S-treated osteoblasts, there were increases in the expression of N-formyl peptide receptor 2 and heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1 and reduction in the expression of alpha-spectrin, prohibitin, and transcription elongation factor B (SIII), polypeptide 1. The identified proteins are associated with cell proliferation, differentiation, signal transcription, and cell growth. These findings might provide valuable insights into the mechanism of antiosteoporotic effect affected by HQSXD treatment in osteoblasts. |
| doi_str_mv | 10.1155/2015/514063 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4628673</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A458645636</galeid><sourcerecordid>A458645636</sourcerecordid><originalsourceid>FETCH-LOGICAL-c528t-aa1c1a1e8546c84a90f66106b0a3af5ae407c841ee544b72756b648d02fee3aa3</originalsourceid><addsrcrecordid>eNqNkk1vEzEQhlcIRD_gxB1Z4oKKQm2vP3YvSFEoFKmoCIrEzZp1ZhNXGzu1vUD5C_xpHFJC4QInW_Yzz9ijt6oeMfqcMSmPOWXyWDJBVX2n2mdasIngTXN3t9ef9qqDlC4p5a3W-n61x5WUmol2v_o-9dmFlDGsQwzZWXLS92hzIqEnpyP4xZUjH8B_deDJS7TBFtwT58lsHPIYcU7eQybnG0M3QCqF3TV5V1QYVsU2W0IEmzG6b_CzsmjzEskFxAVmAn5O3qJdgndp9aC618OQ8OHNelh9fHVyMTudnJ2_fjObnk2s5E2eADDLgGEjhbKNgJb2SjGqOgo19BJQUF3OGaIUotNcS9Up0cwp7xFrgPqwerH1rsduhXOLPkcYzDq6FcRrE8CZP2-8W5pF-GyE4o3SdRE8vRHEcDViymblksVhAI9hTIY1ta6Z0Lz9N6prLttWCFbQJ3-hl2GMvkyiUKUxrVvBf1MLGNA434fyRLuRmqmQjRJS1apQz7aUjSGliP3ud4yaTWrMJjVmm5pCP749kB37KyYFONoCS-fn8MX9nw0Lgj3cghXfED8AF4zUbQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1728603942</pqid></control><display><type>article</type><title>Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>Wiley-Blackwell Open Access Titles</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Zhou, Zhi-Kun ; Zhou, Yan-Xing ; Zhu, Jian-Hong ; Fu, Jian-Ying ; Zheng, Li-Hua ; Guo, Chong-Chong ; Zeng, Tao</creator><contributor>Heese, Klaus</contributor><creatorcontrib>Zhou, Zhi-Kun ; Zhou, Yan-Xing ; Zhu, Jian-Hong ; Fu, Jian-Ying ; Zheng, Li-Hua ; Guo, Chong-Chong ; Zeng, Tao ; Heese, Klaus</creatorcontrib><description>Huangqi Sanxian decoction (HQSXD) is routinely used for the treatment of osteoporosis in the Chinese traditional healthcare system. However, the targets and mechanism underlying the effect of HQSXD on osteoporosis have not been documented. In the present study, seropharmacology and proteomic approaches (two-dimensional gel electrophoresis combined with mass spectrometry) were used to investigate the effects and possible target proteins of HQSXD on osteoblast. We found that HQSXD-treated rat serum significantly enhanced osteoblast proliferation, differentiation, and mineralization. In HQSXD-S-treated osteoblasts, there were increases in the expression of N-formyl peptide receptor 2 and heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1 and reduction in the expression of alpha-spectrin, prohibitin, and transcription elongation factor B (SIII), polypeptide 1. The identified proteins are associated with cell proliferation, differentiation, signal transcription, and cell growth. These findings might provide valuable insights into the mechanism of antiosteoporotic effect affected by HQSXD treatment in osteoblasts.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2015/514063</identifier><identifier>PMID: 26557149</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Cell cycle ; Cell differentiation ; Chemical properties ; Chinese medicine ; Colleges & universities ; Kinases ; Mass spectrometry ; Membrane proteins ; Osteoporosis</subject><ispartof>Evidence-based complementary and alternative medicine, 2015-01, Vol.2015 (2015), p.1-10</ispartof><rights>Copyright © 2015 Chong-Chong Guo et al.</rights><rights>COPYRIGHT 2015 John Wiley & Sons, Inc.</rights><rights>Copyright © 2015 Chong-Chong Guo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2015 Chong-Chong Guo et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-aa1c1a1e8546c84a90f66106b0a3af5ae407c841ee544b72756b648d02fee3aa3</citedby><cites>FETCH-LOGICAL-c528t-aa1c1a1e8546c84a90f66106b0a3af5ae407c841ee544b72756b648d02fee3aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4628673/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4628673/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26557149$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Heese, Klaus</contributor><creatorcontrib>Zhou, Zhi-Kun</creatorcontrib><creatorcontrib>Zhou, Yan-Xing</creatorcontrib><creatorcontrib>Zhu, Jian-Hong</creatorcontrib><creatorcontrib>Fu, Jian-Ying</creatorcontrib><creatorcontrib>Zheng, Li-Hua</creatorcontrib><creatorcontrib>Guo, Chong-Chong</creatorcontrib><creatorcontrib>Zeng, Tao</creatorcontrib><title>Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Huangqi Sanxian decoction (HQSXD) is routinely used for the treatment of osteoporosis in the Chinese traditional healthcare system. However, the targets and mechanism underlying the effect of HQSXD on osteoporosis have not been documented. In the present study, seropharmacology and proteomic approaches (two-dimensional gel electrophoresis combined with mass spectrometry) were used to investigate the effects and possible target proteins of HQSXD on osteoblast. We found that HQSXD-treated rat serum significantly enhanced osteoblast proliferation, differentiation, and mineralization. In HQSXD-S-treated osteoblasts, there were increases in the expression of N-formyl peptide receptor 2 and heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1 and reduction in the expression of alpha-spectrin, prohibitin, and transcription elongation factor B (SIII), polypeptide 1. The identified proteins are associated with cell proliferation, differentiation, signal transcription, and cell growth. These findings might provide valuable insights into the mechanism of antiosteoporotic effect affected by HQSXD treatment in osteoblasts.</description><subject>Cell cycle</subject><subject>Cell differentiation</subject><subject>Chemical properties</subject><subject>Chinese medicine</subject><subject>Colleges & universities</subject><subject>Kinases</subject><subject>Mass spectrometry</subject><subject>Membrane proteins</subject><subject>Osteoporosis</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkk1vEzEQhlcIRD_gxB1Z4oKKQm2vP3YvSFEoFKmoCIrEzZp1ZhNXGzu1vUD5C_xpHFJC4QInW_Yzz9ijt6oeMfqcMSmPOWXyWDJBVX2n2mdasIngTXN3t9ef9qqDlC4p5a3W-n61x5WUmol2v_o-9dmFlDGsQwzZWXLS92hzIqEnpyP4xZUjH8B_deDJS7TBFtwT58lsHPIYcU7eQybnG0M3QCqF3TV5V1QYVsU2W0IEmzG6b_CzsmjzEskFxAVmAn5O3qJdgndp9aC618OQ8OHNelh9fHVyMTudnJ2_fjObnk2s5E2eADDLgGEjhbKNgJb2SjGqOgo19BJQUF3OGaIUotNcS9Up0cwp7xFrgPqwerH1rsduhXOLPkcYzDq6FcRrE8CZP2-8W5pF-GyE4o3SdRE8vRHEcDViymblksVhAI9hTIY1ta6Z0Lz9N6prLttWCFbQJ3-hl2GMvkyiUKUxrVvBf1MLGNA434fyRLuRmqmQjRJS1apQz7aUjSGliP3ud4yaTWrMJjVmm5pCP749kB37KyYFONoCS-fn8MX9nw0Lgj3cghXfED8AF4zUbQ</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Zhou, Zhi-Kun</creator><creator>Zhou, Yan-Xing</creator><creator>Zhu, Jian-Hong</creator><creator>Fu, Jian-Ying</creator><creator>Zheng, Li-Hua</creator><creator>Guo, Chong-Chong</creator><creator>Zeng, Tao</creator><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QP</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism</title><author>Zhou, Zhi-Kun ; Zhou, Yan-Xing ; Zhu, Jian-Hong ; Fu, Jian-Ying ; Zheng, Li-Hua ; Guo, Chong-Chong ; Zeng, Tao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-aa1c1a1e8546c84a90f66106b0a3af5ae407c841ee544b72756b648d02fee3aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Cell cycle</topic><topic>Cell differentiation</topic><topic>Chemical properties</topic><topic>Chinese medicine</topic><topic>Colleges & universities</topic><topic>Kinases</topic><topic>Mass spectrometry</topic><topic>Membrane proteins</topic><topic>Osteoporosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Zhi-Kun</creatorcontrib><creatorcontrib>Zhou, Yan-Xing</creatorcontrib><creatorcontrib>Zhu, Jian-Hong</creatorcontrib><creatorcontrib>Fu, Jian-Ying</creatorcontrib><creatorcontrib>Zheng, Li-Hua</creatorcontrib><creatorcontrib>Guo, Chong-Chong</creatorcontrib><creatorcontrib>Zeng, Tao</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Psychology Database (ProQuest)</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Zhi-Kun</au><au>Zhou, Yan-Xing</au><au>Zhu, Jian-Hong</au><au>Fu, Jian-Ying</au><au>Zheng, Li-Hua</au><au>Guo, Chong-Chong</au><au>Zeng, Tao</au><au>Heese, Klaus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>2015</volume><issue>2015</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Huangqi Sanxian decoction (HQSXD) is routinely used for the treatment of osteoporosis in the Chinese traditional healthcare system. However, the targets and mechanism underlying the effect of HQSXD on osteoporosis have not been documented. In the present study, seropharmacology and proteomic approaches (two-dimensional gel electrophoresis combined with mass spectrometry) were used to investigate the effects and possible target proteins of HQSXD on osteoblast. We found that HQSXD-treated rat serum significantly enhanced osteoblast proliferation, differentiation, and mineralization. In HQSXD-S-treated osteoblasts, there were increases in the expression of N-formyl peptide receptor 2 and heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1 and reduction in the expression of alpha-spectrin, prohibitin, and transcription elongation factor B (SIII), polypeptide 1. The identified proteins are associated with cell proliferation, differentiation, signal transcription, and cell growth. These findings might provide valuable insights into the mechanism of antiosteoporotic effect affected by HQSXD treatment in osteoblasts.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>26557149</pmid><doi>10.1155/2015/514063</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1741-427X |
| ispartof | Evidence-based complementary and alternative medicine, 2015-01, Vol.2015 (2015), p.1-10 |
| issn | 1741-427X 1741-4288 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4628673 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley-Blackwell Open Access Titles; PubMed Central; Alma/SFX Local Collection |
| subjects | Cell cycle Cell differentiation Chemical properties Chinese medicine Colleges & universities Kinases Mass spectrometry Membrane proteins Osteoporosis |
| title | Antiosteoporotic Effects of Huangqi Sanxian Decoction in Cultured Rat Osteoblasts by Proteomic Characterization of the Target and Mechanism |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T17%3A01%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antiosteoporotic%20Effects%20of%20Huangqi%20Sanxian%20Decoction%20in%20Cultured%20Rat%20Osteoblasts%20by%20Proteomic%20Characterization%20of%20the%20Target%20and%20Mechanism&rft.jtitle=Evidence-based%20complementary%20and%20alternative%20medicine&rft.au=Zhou,%20Zhi-Kun&rft.date=2015-01-01&rft.volume=2015&rft.issue=2015&rft.spage=1&rft.epage=10&rft.pages=1-10&rft.issn=1741-427X&rft.eissn=1741-4288&rft_id=info:doi/10.1155/2015/514063&rft_dat=%3Cgale_pubme%3EA458645636%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1728603942&rft_id=info:pmid/26557149&rft_galeid=A458645636&rfr_iscdi=true |