Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models

The in vitro toxicological evaluation of e-liquid aerosol is an important aspect of consumer protection, but the cell model is of great significance. Due to its water solubility, e-liquid aerosol is deposited in the conducting zone of the respiratory tract. Therefore, primary normal human bronchial...

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Veröffentlicht in:International journal of environmental research and public health 2015-10, Vol.12 (10), p.12466-12474
Hauptverfasser: Scheffler, Stefanie, Dieken, Hauke, Krischenowski, Olaf, Aufderheide, Michaela
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container_issue 10
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container_title International journal of environmental research and public health
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creator Scheffler, Stefanie
Dieken, Hauke
Krischenowski, Olaf
Aufderheide, Michaela
description The in vitro toxicological evaluation of e-liquid aerosol is an important aspect of consumer protection, but the cell model is of great significance. Due to its water solubility, e-liquid aerosol is deposited in the conducting zone of the respiratory tract. Therefore, primary normal human bronchial epithelial (NHBE) cells are more suitable for e-liquid aerosol testing than the widely used alveolar cell line A549. Due to their prolonged lifespan, immortalized cell lines derived from primary NHBE cells, exhibiting a comparable in vitro differentiation, might be an alternative for acute toxicity testing. In our study, A549 cells freshly isolated NHBE cells and the immortalized cell line CL-1548 were exposed at the air-liquid interface to e-liquid aerosol and cigarette mainstream smoke in a CULTEX(®) RFS compact module. The cell viability was analyzed 24 h post-exposure. In comparison with primary NHBE cells, the CL-1548 cell line showed lower sensitivity to e-liquid aerosol but significantly higher sensitivity compared to A549 cells. Therefore, the immortalized cell line CL-1548 is recommended as a tool for the routine testing of e-liquid aerosol and is preferable to A549 cells.
doi_str_mv 10.3390/ijerph121012466
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subjects Aerosols
Cell Line
Cell Line, Tumor
Cell Survival - drug effects
Cytotoxicity
Electronic cigarettes
Electronic Nicotine Delivery Systems - adverse effects
Epithelial Cells - drug effects
Humans
Lung - cytology
Smoke - adverse effects
Studies
Testing
Tobacco Products
Toxicity Tests, Acute - methods
Toxicology
title Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models
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