An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative bacteria

An improved vector for chromosomally-integrated promoter-lux fusions is described. The modified vector was tested in parallel with the unmodified vector using the well-characterized Escherichia coli araBAD promoter in the Pseudomonas aeruginosa attTn7 site. The modified mini-Tn7 showed reduced backg...

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Veröffentlicht in:	Journal of microbiological methods 2015-11, Vol.118, p.75-77
Hauptverfasser:	Glassing, Angela, Lewis, Thomas A.
Format:	Artikel
Sprache:	eng
Schlagworte:	araBAD Artificial Gene Fusion DNA Transposable Elements Escherichia coli Gene Expression Profiling - methods Genetics, Microbial - methods Gram-Negative Bacteria - genetics Host Specificity Luciferase Luciferases - analysis Luciferases - genetics Luminescence lux Molecular Biology - methods Promoter Regions, Genetic Pseudomonas aeruginosa Reporter Tn7 Transcriptional Activation
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