Green Fluorescent Protein with Anionic Tryptophan-Based Chromophore and Long Fluorescence Lifetime

Spectral diversity of fluorescent proteins, crucial for multiparameter imaging, is based mainly on chemical diversity of their chromophores. Recently we have reported, to our knowledge, a new green fluorescent protein WasCFP—the first fluorescent protein with a tryptophan-based chromophore in the an...

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Veröffentlicht in:	Biophysical journal 2015-07, Vol.109 (2), p.380-389
Hauptverfasser:	Sarkisyan, Karen S., Goryashchenko, Alexander S., Lidsky, Peter V., Gorbachev, Dmitry A., Bozhanova, Nina G., Gorokhovatsky, Andrey Yu, Pereverzeva, Alina R., Ryumina, Alina P., Zherdeva, Victoria V., Savitsky, Alexander P., Solntsev, Kyril M., Bommarius, Andreas S., Sharonov, George V., Lindquist, Jake R., Drobizhev, Mikhail, Hughes, Thomas E., Rebane, Aleksander, Lukyanov, Konstantin A., Mishin, Alexander S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Genetically Modified Anions - chemistry Cells Drosophila Escherichia coli Fluorescence Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism HEK293 Cells HeLa Cells Humans Hydrogen-Ion Concentration Insects Microscopy, Confocal Molecules Mutation Photochemical Processes Proteins Proteins and Nucleic Acids Temperature Tryptophan - chemistry Tryptophan - metabolism Tumors
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