Detection of Differentially Expressed MicroRNAs in Rheumatic Heart Disease: miR-1183 and miR-1299 as Potential Diagnostic Biomarkers
This study compared microRNA (miRNA) expression profiles between rheumatic heart disease (RHD) patients and healthy controls to investigate their differential expression and help elucidate their mechanisms of action. Microarray analysis was used to measure miRNA expression, and a total of 133 miRNAs...
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| creator | Zhou, Qingyun Xu, Guodong Incoom, Enchill KoJo Zhou, Jianqing Shao, Guofeng Sun, Lebo Shi, Xinbao Huang, Xiaoyan Yang, Xi Zheng, Dawei Zhao, Sheng Lian, Jiangfang Li, Ni Shi, Huoshun |
| description | This study compared microRNA (miRNA) expression profiles between rheumatic heart disease (RHD) patients and healthy controls to investigate their differential expression and help elucidate their mechanisms of action. Microarray analysis was used to measure miRNA expression, and a total of 133 miRNAs were shown to be significantly upregulated in RHD patients compared with controls, including miR-1183 and miR-1299. A total of 137 miRNAs, including miR-4423-3p and miR-218-1-3p, were significantly downregulated in RHD patients. Quantitative real-time-PCR confirmed microarray findings for miR-1183 and miR-1299 in both tissue and plasma. Bioinformatic predictions were also made of differentially expressed miRNAs as biomarkers in RHD by databases and GO/pathway analysis. Furthermore, we investigated miR-1183 and miR-1299 expression in RHD patients with secondary pulmonary hypertension (PAH). Our findings identified an important role for miR-1299 as a direct regulator of RHD, while the observed difference in expression of miR-1183 between RHD-PAH patients with high or low pulmonary artery pressure suggests that miR-1183 overexpression may reflect pulmonary artery remodeling. miR-1183 and miR-1299 appear to play distinct roles in RHD pathogenesis accompanied by secondary PAH and could be used as potential biological markers for disease development. |
| doi_str_mv | 10.1155/2015/524519 |
| format | Article |
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Microarray analysis was used to measure miRNA expression, and a total of 133 miRNAs were shown to be significantly upregulated in RHD patients compared with controls, including miR-1183 and miR-1299. A total of 137 miRNAs, including miR-4423-3p and miR-218-1-3p, were significantly downregulated in RHD patients. Quantitative real-time-PCR confirmed microarray findings for miR-1183 and miR-1299 in both tissue and plasma. Bioinformatic predictions were also made of differentially expressed miRNAs as biomarkers in RHD by databases and GO/pathway analysis. Furthermore, we investigated miR-1183 and miR-1299 expression in RHD patients with secondary pulmonary hypertension (PAH). Our findings identified an important role for miR-1299 as a direct regulator of RHD, while the observed difference in expression of miR-1183 between RHD-PAH patients with high or low pulmonary artery pressure suggests that miR-1183 overexpression may reflect pulmonary artery remodeling. miR-1183 and miR-1299 appear to play distinct roles in RHD pathogenesis accompanied by secondary PAH and could be used as potential biological markers for disease development.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2015/524519</identifier><identifier>PMID: 26539505</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Apoptosis ; Bioinformatics ; Biomarkers ; Biomarkers - blood ; Cardiovascular disease ; Comparative analysis ; Computational Biology ; Family medical history ; Female ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation ; Genetic aspects ; Health aspects ; Heart failure ; Hospitals ; Humans ; Hypertension ; Hypertension, Pulmonary - blood ; Hypertension, Pulmonary - genetics ; Hypertension, Pulmonary - pathology ; Male ; Methods ; MicroRNA ; MicroRNAs ; MicroRNAs - blood ; MicroRNAs - genetics ; Observations ; Oligonucleotide Array Sequence Analysis ; Physiology ; Pulmonary arteries ; Rheumatic heart disease ; Rheumatic Heart Disease - blood ; Rheumatic Heart Disease - genetics ; Rheumatic Heart Disease - pathology ; RNA sequencing ; Studies</subject><ispartof>BioMed research international, 2015-01, Vol.2015 (2015), p.1-11</ispartof><rights>Copyright © 2015 Ni Li et al.</rights><rights>COPYRIGHT 2015 John Wiley & Sons, Inc.</rights><rights>Copyright © 2015 Ni Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2015 Ni Li et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c594t-4960d675b370cd1d725fdbe083519ca40534aef2a7b1e5ca38b60f1b0112ac6b3</citedby><cites>FETCH-LOGICAL-c594t-4960d675b370cd1d725fdbe083519ca40534aef2a7b1e5ca38b60f1b0112ac6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619814/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619814/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26539505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Di Raimondo, Francesco</contributor><creatorcontrib>Zhou, Qingyun</creatorcontrib><creatorcontrib>Xu, Guodong</creatorcontrib><creatorcontrib>Incoom, Enchill KoJo</creatorcontrib><creatorcontrib>Zhou, Jianqing</creatorcontrib><creatorcontrib>Shao, Guofeng</creatorcontrib><creatorcontrib>Sun, Lebo</creatorcontrib><creatorcontrib>Shi, Xinbao</creatorcontrib><creatorcontrib>Huang, Xiaoyan</creatorcontrib><creatorcontrib>Yang, Xi</creatorcontrib><creatorcontrib>Zheng, Dawei</creatorcontrib><creatorcontrib>Zhao, Sheng</creatorcontrib><creatorcontrib>Lian, Jiangfang</creatorcontrib><creatorcontrib>Li, Ni</creatorcontrib><creatorcontrib>Shi, Huoshun</creatorcontrib><title>Detection of Differentially Expressed MicroRNAs in Rheumatic Heart Disease: miR-1183 and miR-1299 as Potential Diagnostic Biomarkers</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>This study compared microRNA (miRNA) expression profiles between rheumatic heart disease (RHD) patients and healthy controls to investigate their differential expression and help elucidate their mechanisms of action. Microarray analysis was used to measure miRNA expression, and a total of 133 miRNAs were shown to be significantly upregulated in RHD patients compared with controls, including miR-1183 and miR-1299. A total of 137 miRNAs, including miR-4423-3p and miR-218-1-3p, were significantly downregulated in RHD patients. Quantitative real-time-PCR confirmed microarray findings for miR-1183 and miR-1299 in both tissue and plasma. Bioinformatic predictions were also made of differentially expressed miRNAs as biomarkers in RHD by databases and GO/pathway analysis. Furthermore, we investigated miR-1183 and miR-1299 expression in RHD patients with secondary pulmonary hypertension (PAH). Our findings identified an important role for miR-1299 as a direct regulator of RHD, while the observed difference in expression of miR-1183 between RHD-PAH patients with high or low pulmonary artery pressure suggests that miR-1183 overexpression may reflect pulmonary artery remodeling. miR-1183 and miR-1299 appear to play distinct roles in RHD pathogenesis accompanied by secondary PAH and could be used as potential biological markers for disease development.</description><subject>Apoptosis</subject><subject>Bioinformatics</subject><subject>Biomarkers</subject><subject>Biomarkers - blood</subject><subject>Cardiovascular disease</subject><subject>Comparative analysis</subject><subject>Computational Biology</subject><subject>Family medical history</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Heart failure</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Hypertension</subject><subject>Hypertension, Pulmonary - blood</subject><subject>Hypertension, Pulmonary - genetics</subject><subject>Hypertension, Pulmonary - pathology</subject><subject>Male</subject><subject>Methods</subject><subject>MicroRNA</subject><subject>MicroRNAs</subject><subject>MicroRNAs - blood</subject><subject>MicroRNAs - genetics</subject><subject>Observations</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Physiology</subject><subject>Pulmonary arteries</subject><subject>Rheumatic heart disease</subject><subject>Rheumatic Heart Disease - blood</subject><subject>Rheumatic Heart Disease - genetics</subject><subject>Rheumatic Heart Disease - pathology</subject><subject>RNA sequencing</subject><subject>Studies</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNks1v1DAQxSMEolXpiTuyxAWBQj3-SsKh0tIWilQ-tIKz5TiTXZckXuykpXf-cLxKWQqn-mJb_vnpzczLsqdAXwNIecQoyCPJhITqQbbPOIhcgYCHuzPne9lhjJc0rRIUrdTjbI8pyStJ5X726xRHtKPzA_EtOXVtiwGH0ZmuuyFnPzcBY8SGfHQ2-OWnRSRuIMs1Tr0ZnSXnaMKYfkU0Ed-Q3i1zgJITMzTzhVUVMZF88eMsmlizGnzcfn7rfG_CdwzxSfaoNV3Ew9v9IPv27uzryXl-8fn9h5PFRW5lJcZcVIo2qpA1L6htoCmYbJsaaclT9dYIKrkw2DJT1IDSGl7WirZQUwBmrKr5QXY8626musfGJk_BdHoTXDJyo71x-t-Xwa31yl9poaAqQSSBF7cCwf-YMI66d9Fi15kB_RQ1FJyVDFgp74NCUXEAldDn_6GXfgpD6kSimFKloIL9pVamQ-2G1ieLdiuqF0Km0UJJq0S9mqk0rxgDtrvqgOptYvQ2MXpOTKKf3W3Ijv2TjwS8nIG1Gxpz7e6nhgnB1tyBZTIo-W9VIc9e</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Zhou, Qingyun</creator><creator>Xu, Guodong</creator><creator>Incoom, Enchill KoJo</creator><creator>Zhou, Jianqing</creator><creator>Shao, Guofeng</creator><creator>Sun, Lebo</creator><creator>Shi, Xinbao</creator><creator>Huang, Xiaoyan</creator><creator>Yang, Xi</creator><creator>Zheng, Dawei</creator><creator>Zhao, Sheng</creator><creator>Lian, Jiangfang</creator><creator>Li, Ni</creator><creator>Shi, Huoshun</creator><general>Hindawi Publishing Corporation</general><general>John Wiley & Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>Detection of Differentially Expressed MicroRNAs in Rheumatic Heart Disease: miR-1183 and miR-1299 as Potential Diagnostic Biomarkers</title><author>Zhou, Qingyun ; Xu, Guodong ; Incoom, Enchill KoJo ; Zhou, Jianqing ; Shao, Guofeng ; Sun, Lebo ; Shi, Xinbao ; Huang, Xiaoyan ; Yang, Xi ; Zheng, Dawei ; Zhao, Sheng ; Lian, Jiangfang ; Li, Ni ; Shi, Huoshun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c594t-4960d675b370cd1d725fdbe083519ca40534aef2a7b1e5ca38b60f1b0112ac6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Apoptosis</topic><topic>Bioinformatics</topic><topic>Biomarkers</topic><topic>Biomarkers - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Qingyun</au><au>Xu, Guodong</au><au>Incoom, Enchill KoJo</au><au>Zhou, Jianqing</au><au>Shao, Guofeng</au><au>Sun, Lebo</au><au>Shi, Xinbao</au><au>Huang, Xiaoyan</au><au>Yang, Xi</au><au>Zheng, Dawei</au><au>Zhao, Sheng</au><au>Lian, Jiangfang</au><au>Li, Ni</au><au>Shi, Huoshun</au><au>Di Raimondo, Francesco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Differentially Expressed MicroRNAs in Rheumatic Heart Disease: miR-1183 and miR-1299 as Potential Diagnostic Biomarkers</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>2015</volume><issue>2015</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>This study compared microRNA (miRNA) expression profiles between rheumatic heart disease (RHD) patients and healthy controls to investigate their differential expression and help elucidate their mechanisms of action. Microarray analysis was used to measure miRNA expression, and a total of 133 miRNAs were shown to be significantly upregulated in RHD patients compared with controls, including miR-1183 and miR-1299. A total of 137 miRNAs, including miR-4423-3p and miR-218-1-3p, were significantly downregulated in RHD patients. Quantitative real-time-PCR confirmed microarray findings for miR-1183 and miR-1299 in both tissue and plasma. Bioinformatic predictions were also made of differentially expressed miRNAs as biomarkers in RHD by databases and GO/pathway analysis. Furthermore, we investigated miR-1183 and miR-1299 expression in RHD patients with secondary pulmonary hypertension (PAH). Our findings identified an important role for miR-1299 as a direct regulator of RHD, while the observed difference in expression of miR-1183 between RHD-PAH patients with high or low pulmonary artery pressure suggests that miR-1183 overexpression may reflect pulmonary artery remodeling. miR-1183 and miR-1299 appear to play distinct roles in RHD pathogenesis accompanied by secondary PAH and could be used as potential biological markers for disease development.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>26539505</pmid><doi>10.1155/2015/524519</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apoptosis Bioinformatics Biomarkers Biomarkers - blood Cardiovascular disease Comparative analysis Computational Biology Family medical history Female Gene expression Gene Expression Profiling Gene Expression Regulation Genetic aspects Health aspects Heart failure Hospitals Humans Hypertension Hypertension, Pulmonary - blood Hypertension, Pulmonary - genetics Hypertension, Pulmonary - pathology Male Methods MicroRNA MicroRNAs MicroRNAs - blood MicroRNAs - genetics Observations Oligonucleotide Array Sequence Analysis Physiology Pulmonary arteries Rheumatic heart disease Rheumatic Heart Disease - blood Rheumatic Heart Disease - genetics Rheumatic Heart Disease - pathology RNA sequencing Studies |
| title | Detection of Differentially Expressed MicroRNAs in Rheumatic Heart Disease: miR-1183 and miR-1299 as Potential Diagnostic Biomarkers |
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