Characterization of Shiga Toxigenic Escherichia coli O157 and Non-O157 Isolates from Ruminant Feces in Malaysia
Shiga toxigenic Escherichia coli (STEC) O157 and several other serogroups of non-O157 STEC are causative agents of severe disease in humans world-wide. The present study was conducted to characterize STEC O157 and non-O157 serogroups O26, O103, O111, O121, O45, and O145 in ruminants in Malaysia. A t...
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| creator | Fegan, Narelle Dykes, Gary Clarke, Charles M. Perera, Asanthi |
| description | Shiga toxigenic Escherichia coli (STEC) O157 and several other serogroups of non-O157 STEC are causative agents of severe disease in humans world-wide. The present study was conducted to characterize STEC O157 and non-O157 serogroups O26, O103, O111, O121, O45, and O145 in ruminants in Malaysia. A total of 136 ruminant feces samples were collected from 6 different farms in Peninsular Malaysia. Immunomagnetic beads were used to isolate E. coli O157 and non-O157 serogroups, while PCR was used for the detection and subtyping of STEC isolates. STEC O157:H7 was isolated from 6 (4%) feces samples and all isolates obtained carried stx2c, eaeA-γ1, and ehxA. Non-O157 STEC was isolated from 2 (1.5%) feces samples with one isolate carrying stx1a, stx2a, stx2c, and ehxA and the other carrying stx1a alone. The presence of STEC O157 and non-O157 in a small percentage of ruminants in this study together with their virulence characteristics suggests that they may have limited impact on public health. |
| doi_str_mv | 10.1155/2015/382403 |
| format | Article |
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The present study was conducted to characterize STEC O157 and non-O157 serogroups O26, O103, O111, O121, O45, and O145 in ruminants in Malaysia. A total of 136 ruminant feces samples were collected from 6 different farms in Peninsular Malaysia. Immunomagnetic beads were used to isolate E. coli O157 and non-O157 serogroups, while PCR was used for the detection and subtyping of STEC isolates. STEC O157:H7 was isolated from 6 (4%) feces samples and all isolates obtained carried stx2c, eaeA-γ1, and ehxA. Non-O157 STEC was isolated from 2 (1.5%) feces samples with one isolate carrying stx1a, stx2a, stx2c, and ehxA and the other carrying stx1a alone. The presence of STEC O157 and non-O157 in a small percentage of ruminants in this study together with their virulence characteristics suggests that they may have limited impact on public health.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2015/382403</identifier><identifier>PMID: 26539484</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Animals ; Bacteriology ; Biomedical research ; Cattle ; Escherichia coli ; Escherichia coli Infections - genetics ; Escherichia coli Infections - microbiology ; Escherichia coli O157 - genetics ; Escherichia coli O157 - pathogenicity ; Escherichia coli Proteins - genetics ; Feces ; Feces - microbiology ; Food Microbiology ; Health aspects ; Hemolysin Proteins - genetics ; Humans ; Malaysia ; Ruminantia ; Ruminants - microbiology ; Serotyping ; Shiga Toxin - genetics ; Shiga-Toxigenic Escherichia coli - genetics ; Shiga-Toxigenic Escherichia coli - pathogenicity ; Studies</subject><ispartof>BioMed research international, 2015-01, Vol.2015 (2015), p.1-8</ispartof><rights>Copyright © 2015 Asanthi Perera et al.</rights><rights>COPYRIGHT 2015 John Wiley & Sons, Inc.</rights><rights>Copyright © 2015 Asanthi Perera et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2015 Asanthi Perera et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-7637c22c09ff667741ff3864da17c5e5d670e67f0d2ac332e406c3bc73e8ee3e3</citedby><cites>FETCH-LOGICAL-c491t-7637c22c09ff667741ff3864da17c5e5d670e67f0d2ac332e406c3bc73e8ee3e3</cites><orcidid>0000-0001-5014-9282</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619789/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4619789/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26539484$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Prieto, Miguel</contributor><creatorcontrib>Fegan, Narelle</creatorcontrib><creatorcontrib>Dykes, Gary</creatorcontrib><creatorcontrib>Clarke, Charles M.</creatorcontrib><creatorcontrib>Perera, Asanthi</creatorcontrib><title>Characterization of Shiga Toxigenic Escherichia coli O157 and Non-O157 Isolates from Ruminant Feces in Malaysia</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Shiga toxigenic Escherichia coli (STEC) O157 and several other serogroups of non-O157 STEC are causative agents of severe disease in humans world-wide. The present study was conducted to characterize STEC O157 and non-O157 serogroups O26, O103, O111, O121, O45, and O145 in ruminants in Malaysia. A total of 136 ruminant feces samples were collected from 6 different farms in Peninsular Malaysia. Immunomagnetic beads were used to isolate E. coli O157 and non-O157 serogroups, while PCR was used for the detection and subtyping of STEC isolates. STEC O157:H7 was isolated from 6 (4%) feces samples and all isolates obtained carried stx2c, eaeA-γ1, and ehxA. Non-O157 STEC was isolated from 2 (1.5%) feces samples with one isolate carrying stx1a, stx2a, stx2c, and ehxA and the other carrying stx1a alone. 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BioMed research international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fegan, Narelle</au><au>Dykes, Gary</au><au>Clarke, Charles M.</au><au>Perera, Asanthi</au><au>Prieto, Miguel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Shiga Toxigenic Escherichia coli O157 and Non-O157 Isolates from Ruminant Feces in Malaysia</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>2015</volume><issue>2015</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Shiga toxigenic Escherichia coli (STEC) O157 and several other serogroups of non-O157 STEC are causative agents of severe disease in humans world-wide. The present study was conducted to characterize STEC O157 and non-O157 serogroups O26, O103, O111, O121, O45, and O145 in ruminants in Malaysia. A total of 136 ruminant feces samples were collected from 6 different farms in Peninsular Malaysia. Immunomagnetic beads were used to isolate E. coli O157 and non-O157 serogroups, while PCR was used for the detection and subtyping of STEC isolates. STEC O157:H7 was isolated from 6 (4%) feces samples and all isolates obtained carried stx2c, eaeA-γ1, and ehxA. Non-O157 STEC was isolated from 2 (1.5%) feces samples with one isolate carrying stx1a, stx2a, stx2c, and ehxA and the other carrying stx1a alone. The presence of STEC O157 and non-O157 in a small percentage of ruminants in this study together with their virulence characteristics suggests that they may have limited impact on public health.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>26539484</pmid><doi>10.1155/2015/382403</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-5014-9282</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bacteriology Biomedical research Cattle Escherichia coli Escherichia coli Infections - genetics Escherichia coli Infections - microbiology Escherichia coli O157 - genetics Escherichia coli O157 - pathogenicity Escherichia coli Proteins - genetics Feces Feces - microbiology Food Microbiology Health aspects Hemolysin Proteins - genetics Humans Malaysia Ruminantia Ruminants - microbiology Serotyping Shiga Toxin - genetics Shiga-Toxigenic Escherichia coli - genetics Shiga-Toxigenic Escherichia coli - pathogenicity Studies |
| title | Characterization of Shiga Toxigenic Escherichia coli O157 and Non-O157 Isolates from Ruminant Feces in Malaysia |
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