Icariin protects rats against 5/6 nephrectomy-induced chronic kidney failure by increasing the number of renal stem cells
Chronic kidney disease poses a serious health problem worldwide with increasing prevalence and lack of effective treatment. This study aimed to investigate the mechanism of icariin in alleviating chronic renal failure induced by 5/6 nephrectomy in rats. The chronic renal failure model was establishe...
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| Veröffentlicht in: | BMC complementary and alternative medicine 2015-10, Vol.15 (1), p.378-378, Article 378 |
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| creator | Huang, Zhongdi He, Liqun Huang, Di Lei, Shi Gao, Jiandong |
| description | Chronic kidney disease poses a serious health problem worldwide with increasing prevalence and lack of effective treatment. This study aimed to investigate the mechanism of icariin in alleviating chronic renal failure induced by 5/6 nephrectomy in rats.
The chronic renal failure model was established by a two-phased 5/6 nephrectomy procedure. The model rats were given daily doses of water or icariin for 8 weeks. The kidney morphology was checked by HE staining. The levels of blood urea nitrogen, serum creatinine, and serum uric acid were measured by colometric methods. The expression of specified genes was analyzed by quantitative real-time PCR and immunohistochemical staining. The number of renal stem/progenitor cells was analyzed by CD133 and CD24 immunohistochemical staining.
Icariin protected against CDK-caused damages to kidney histology and improved renal function, significantly reduced levels of BUN, creatinine, and uric acid. Icariin inhibited the expression level of TGF-β1 whereas upregulated HGF, BMP-7, WT-1, and Pax2 expression. Moreover, ccariin significantly increased the expression of CD24, CD133, Osr1, and Nanog in remnant kidney and the numbers of CD133(+)/CD24(+) renal stem/progenitor cells.
These data demonstrated that icariin effectively alleviated 5/6 nephrectomy induced chronic renal failure through increasing renal stem/progenitor cells. |
| doi_str_mv | 10.1186/s12906-015-0909-8 |
| format | Article |
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The chronic renal failure model was established by a two-phased 5/6 nephrectomy procedure. The model rats were given daily doses of water or icariin for 8 weeks. The kidney morphology was checked by HE staining. The levels of blood urea nitrogen, serum creatinine, and serum uric acid were measured by colometric methods. The expression of specified genes was analyzed by quantitative real-time PCR and immunohistochemical staining. The number of renal stem/progenitor cells was analyzed by CD133 and CD24 immunohistochemical staining.
Icariin protected against CDK-caused damages to kidney histology and improved renal function, significantly reduced levels of BUN, creatinine, and uric acid. Icariin inhibited the expression level of TGF-β1 whereas upregulated HGF, BMP-7, WT-1, and Pax2 expression. Moreover, ccariin significantly increased the expression of CD24, CD133, Osr1, and Nanog in remnant kidney and the numbers of CD133(+)/CD24(+) renal stem/progenitor cells.
These data demonstrated that icariin effectively alleviated 5/6 nephrectomy induced chronic renal failure through increasing renal stem/progenitor cells.</description><identifier>ISSN: 1472-6882</identifier><identifier>EISSN: 1472-6882</identifier><identifier>DOI: 10.1186/s12906-015-0909-8</identifier><identifier>PMID: 26490949</identifier><language>eng</language><publisher>England: BioMed Central</publisher><subject>Animals ; blood serum ; Blood Urea Nitrogen ; Bone Morphogenetic Protein 7 - genetics ; Bone Morphogenetic Protein 7 - metabolism ; Cell Proliferation - drug effects ; complement ; creatinine ; Creatinine - blood ; Drugs, Chinese Herbal - administration & dosage ; Flavonoids - administration & dosage ; Humans ; icariin ; immunohistochemistry ; Kidney - cytology ; Kidney - drug effects ; Kidney - surgery ; Kidney Failure, Chronic - blood ; Kidney Failure, Chronic - drug therapy ; Kidney Failure, Chronic - physiopathology ; Kidney Failure, Chronic - prevention & control ; kidneys ; Male ; Nephrectomy ; quantitative polymerase chain reaction ; Rats ; Rats, Sprague-Dawley ; renal failure ; renal function ; Stem Cells - cytology ; Stem Cells - drug effects ; Transforming Growth Factor beta1 - genetics ; Transforming Growth Factor beta1 - metabolism ; urea nitrogen ; uric acid ; Uric Acid - blood</subject><ispartof>BMC complementary and alternative medicine, 2015-10, Vol.15 (1), p.378-378, Article 378</ispartof><rights>Copyright BioMed Central 2015</rights><rights>Huang et al. 2015</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-d03436a723477a0a5dcc1e4e7e54c7906827e40015ec4f9d31c0666d06eccd6c3</citedby><cites>FETCH-LOGICAL-c460t-d03436a723477a0a5dcc1e4e7e54c7906827e40015ec4f9d31c0666d06eccd6c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617909/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617909/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26490949$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Zhongdi</creatorcontrib><creatorcontrib>He, Liqun</creatorcontrib><creatorcontrib>Huang, Di</creatorcontrib><creatorcontrib>Lei, Shi</creatorcontrib><creatorcontrib>Gao, Jiandong</creatorcontrib><title>Icariin protects rats against 5/6 nephrectomy-induced chronic kidney failure by increasing the number of renal stem cells</title><title>BMC complementary and alternative medicine</title><addtitle>BMC Complement Altern Med</addtitle><description>Chronic kidney disease poses a serious health problem worldwide with increasing prevalence and lack of effective treatment. This study aimed to investigate the mechanism of icariin in alleviating chronic renal failure induced by 5/6 nephrectomy in rats.
The chronic renal failure model was established by a two-phased 5/6 nephrectomy procedure. The model rats were given daily doses of water or icariin for 8 weeks. The kidney morphology was checked by HE staining. The levels of blood urea nitrogen, serum creatinine, and serum uric acid were measured by colometric methods. The expression of specified genes was analyzed by quantitative real-time PCR and immunohistochemical staining. The number of renal stem/progenitor cells was analyzed by CD133 and CD24 immunohistochemical staining.
Icariin protected against CDK-caused damages to kidney histology and improved renal function, significantly reduced levels of BUN, creatinine, and uric acid. Icariin inhibited the expression level of TGF-β1 whereas upregulated HGF, BMP-7, WT-1, and Pax2 expression. Moreover, ccariin significantly increased the expression of CD24, CD133, Osr1, and Nanog in remnant kidney and the numbers of CD133(+)/CD24(+) renal stem/progenitor cells.
These data demonstrated that icariin effectively alleviated 5/6 nephrectomy induced chronic renal failure through increasing renal stem/progenitor cells.</description><subject>Animals</subject><subject>blood serum</subject><subject>Blood Urea Nitrogen</subject><subject>Bone Morphogenetic Protein 7 - genetics</subject><subject>Bone Morphogenetic Protein 7 - metabolism</subject><subject>Cell Proliferation - drug effects</subject><subject>complement</subject><subject>creatinine</subject><subject>Creatinine - blood</subject><subject>Drugs, Chinese Herbal - administration & dosage</subject><subject>Flavonoids - administration & dosage</subject><subject>Humans</subject><subject>icariin</subject><subject>immunohistochemistry</subject><subject>Kidney - cytology</subject><subject>Kidney - drug effects</subject><subject>Kidney - surgery</subject><subject>Kidney Failure, Chronic - blood</subject><subject>Kidney Failure, Chronic - drug therapy</subject><subject>Kidney Failure, Chronic - physiopathology</subject><subject>Kidney Failure, Chronic - prevention & control</subject><subject>kidneys</subject><subject>Male</subject><subject>Nephrectomy</subject><subject>quantitative polymerase chain reaction</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>renal failure</subject><subject>renal function</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Transforming Growth Factor beta1 - genetics</subject><subject>Transforming Growth Factor beta1 - metabolism</subject><subject>urea nitrogen</subject><subject>uric acid</subject><subject>Uric Acid - blood</subject><issn>1472-6882</issn><issn>1472-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqFkk9v1DAQxS0EoqXwAbggS1y4hNqOY8cXJFTxp1IlLnC2vOPJrktiL3ZSKd8eR1uqwoWLbWl-8zRv_Ah5zdl7znt1WbgwTDWMdw0zzDT9E3LOpRaN6nvx9NH7jLwo5ZYxrnsun5MzoWTlpTkn6zW4HEKkx5xmhLnQ7Orh9i7EMtPuUtGIx0OupTStTYh-AfQUDjnFAPRn8BFXOrgwLhnpbqUhQkZXQtzT-YA0LtMOM00DzRjdSMuMEwUcx_KSPBvcWPDV_X1Bfnz-9P3qa3Pz7cv11cebBqRic-NZK1vltGil1o65zgNwlKixk6Cr_V5olNVahyAH41sOTCnlmUIAr6C9IB9OusdlN6EHjHN2oz3mMLm82uSC_bsSw8Hu052Vild9UwXe3Qvk9GvBMtsplM2Ci5iWYkWdjXMh-v-jXAttjBBSV_TtP-htWnJd0UZpszlTvFL8REFOpWQcHubmzG4ZsKcM2Orfbhmwfe1589jwQ8efT29_A3lNriQ</recordid><startdate>20151021</startdate><enddate>20151021</enddate><creator>Huang, Zhongdi</creator><creator>He, Liqun</creator><creator>Huang, Di</creator><creator>Lei, Shi</creator><creator>Gao, Jiandong</creator><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20151021</creationdate><title>Icariin protects rats against 5/6 nephrectomy-induced chronic kidney failure by increasing the number of renal stem cells</title><author>Huang, Zhongdi ; He, Liqun ; Huang, Di ; Lei, Shi ; Gao, Jiandong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-d03436a723477a0a5dcc1e4e7e54c7906827e40015ec4f9d31c0666d06eccd6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>blood serum</topic><topic>Blood Urea Nitrogen</topic><topic>Bone Morphogenetic Protein 7 - genetics</topic><topic>Bone Morphogenetic Protein 7 - metabolism</topic><topic>Cell Proliferation - drug effects</topic><topic>complement</topic><topic>creatinine</topic><topic>Creatinine - blood</topic><topic>Drugs, Chinese Herbal - administration & dosage</topic><topic>Flavonoids - administration & dosage</topic><topic>Humans</topic><topic>icariin</topic><topic>immunohistochemistry</topic><topic>Kidney - cytology</topic><topic>Kidney - drug effects</topic><topic>Kidney - surgery</topic><topic>Kidney Failure, Chronic - blood</topic><topic>Kidney Failure, Chronic - drug therapy</topic><topic>Kidney Failure, Chronic - physiopathology</topic><topic>Kidney Failure, Chronic - prevention & control</topic><topic>kidneys</topic><topic>Male</topic><topic>Nephrectomy</topic><topic>quantitative polymerase chain reaction</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>renal failure</topic><topic>renal function</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Transforming Growth Factor beta1 - genetics</topic><topic>Transforming Growth Factor beta1 - metabolism</topic><topic>urea nitrogen</topic><topic>uric acid</topic><topic>Uric Acid - blood</topic><toplevel>online_resources</toplevel><creatorcontrib>Huang, Zhongdi</creatorcontrib><creatorcontrib>He, Liqun</creatorcontrib><creatorcontrib>Huang, Di</creatorcontrib><creatorcontrib>Lei, Shi</creatorcontrib><creatorcontrib>Gao, Jiandong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Zhongdi</au><au>He, Liqun</au><au>Huang, Di</au><au>Lei, Shi</au><au>Gao, Jiandong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Icariin protects rats against 5/6 nephrectomy-induced chronic kidney failure by increasing the number of renal stem cells</atitle><jtitle>BMC complementary and alternative medicine</jtitle><addtitle>BMC Complement Altern Med</addtitle><date>2015-10-21</date><risdate>2015</risdate><volume>15</volume><issue>1</issue><spage>378</spage><epage>378</epage><pages>378-378</pages><artnum>378</artnum><issn>1472-6882</issn><eissn>1472-6882</eissn><abstract>Chronic kidney disease poses a serious health problem worldwide with increasing prevalence and lack of effective treatment. This study aimed to investigate the mechanism of icariin in alleviating chronic renal failure induced by 5/6 nephrectomy in rats.
The chronic renal failure model was established by a two-phased 5/6 nephrectomy procedure. The model rats were given daily doses of water or icariin for 8 weeks. The kidney morphology was checked by HE staining. The levels of blood urea nitrogen, serum creatinine, and serum uric acid were measured by colometric methods. The expression of specified genes was analyzed by quantitative real-time PCR and immunohistochemical staining. The number of renal stem/progenitor cells was analyzed by CD133 and CD24 immunohistochemical staining.
Icariin protected against CDK-caused damages to kidney histology and improved renal function, significantly reduced levels of BUN, creatinine, and uric acid. Icariin inhibited the expression level of TGF-β1 whereas upregulated HGF, BMP-7, WT-1, and Pax2 expression. Moreover, ccariin significantly increased the expression of CD24, CD133, Osr1, and Nanog in remnant kidney and the numbers of CD133(+)/CD24(+) renal stem/progenitor cells.
These data demonstrated that icariin effectively alleviated 5/6 nephrectomy induced chronic renal failure through increasing renal stem/progenitor cells.</abstract><cop>England</cop><pub>BioMed Central</pub><pmid>26490949</pmid><doi>10.1186/s12906-015-0909-8</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals blood serum Blood Urea Nitrogen Bone Morphogenetic Protein 7 - genetics Bone Morphogenetic Protein 7 - metabolism Cell Proliferation - drug effects complement creatinine Creatinine - blood Drugs, Chinese Herbal - administration & dosage Flavonoids - administration & dosage Humans icariin immunohistochemistry Kidney - cytology Kidney - drug effects Kidney - surgery Kidney Failure, Chronic - blood Kidney Failure, Chronic - drug therapy Kidney Failure, Chronic - physiopathology Kidney Failure, Chronic - prevention & control kidneys Male Nephrectomy quantitative polymerase chain reaction Rats Rats, Sprague-Dawley renal failure renal function Stem Cells - cytology Stem Cells - drug effects Transforming Growth Factor beta1 - genetics Transforming Growth Factor beta1 - metabolism urea nitrogen uric acid Uric Acid - blood |
| title | Icariin protects rats against 5/6 nephrectomy-induced chronic kidney failure by increasing the number of renal stem cells |
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