Transcriptome analysis of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE)
Infection of the snail, Biomphalaria glabrata, by the free-swimming miracidial stage of the human blood fluke, Schistosoma mansoni, and its subsequent development to the parasitic sporocyst stage is critical to establishment of viable infections and continued human transmission. We performed a genom...
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| Veröffentlicht in: | Parasitology 2009-04, Vol.136 (5), p.469-485 |
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| creator | TAFT, A. S. VERMEIRE, J. J. BERNIER, J. BIRKELAND, S. R. CIPRIANO, M. J. PAPA, A. R. McARTHUR, A. G. YOSHINO, T. P. |
| description | Infection of the snail, Biomphalaria glabrata, by the free-swimming miracidial stage of the human blood fluke, Schistosoma mansoni, and its subsequent development to the parasitic sporocyst stage is critical to establishment of viable infections and continued human transmission. We performed a genome-wide expression analysis of the S. mansoni miracidia and developing sporocyst using Long Serial Analysis of Gene Expression (LongSAGE). Five cDNA libraries were constructed from miracidia and in vitro cultured 6- and 20-day-old sporocysts maintained in sporocyst medium (SM) or in SM conditioned by previous cultivation with cells of the B. glabrata embryonic (Bge) cell line. We generated 21 440 SAGE tags and mapped 13 381 to the S. mansoni gene predictions (v4.0e) either by estimating theoretical 3′ UTR lengths or using existing 3′ EST sequence data. Overall, 432 transcripts were found to be differentially expressed amongst all 5 libraries. In total, 172 tags were differentially expressed between miracidia and 6-day conditioned sporocysts and 152 were differentially expressed between miracidia and 6-day unconditioned sporocysts. In addition, 53 and 45 tags, respectively, were differentially expressed in 6-day and 20-day cultured sporocysts, due to the effects of exposure to Bge cell-conditioned medium. |
| doi_str_mv | 10.1017/S0031182009005733 |
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S. ; VERMEIRE, J. J. ; BERNIER, J. ; BIRKELAND, S. R. ; CIPRIANO, M. J. ; PAPA, A. R. ; McARTHUR, A. G. ; YOSHINO, T. P.</creator><creatorcontrib>TAFT, A. S. ; VERMEIRE, J. J. ; BERNIER, J. ; BIRKELAND, S. R. ; CIPRIANO, M. J. ; PAPA, A. R. ; McARTHUR, A. G. ; YOSHINO, T. P.</creatorcontrib><description>Infection of the snail, Biomphalaria glabrata, by the free-swimming miracidial stage of the human blood fluke, Schistosoma mansoni, and its subsequent development to the parasitic sporocyst stage is critical to establishment of viable infections and continued human transmission. We performed a genome-wide expression analysis of the S. mansoni miracidia and developing sporocyst using Long Serial Analysis of Gene Expression (LongSAGE). Five cDNA libraries were constructed from miracidia and in vitro cultured 6- and 20-day-old sporocysts maintained in sporocyst medium (SM) or in SM conditioned by previous cultivation with cells of the B. glabrata embryonic (Bge) cell line. We generated 21 440 SAGE tags and mapped 13 381 to the S. mansoni gene predictions (v4.0e) either by estimating theoretical 3′ UTR lengths or using existing 3′ EST sequence data. Overall, 432 transcripts were found to be differentially expressed amongst all 5 libraries. In total, 172 tags were differentially expressed between miracidia and 6-day conditioned sporocysts and 152 were differentially expressed between miracidia and 6-day unconditioned sporocysts. In addition, 53 and 45 tags, respectively, were differentially expressed in 6-day and 20-day cultured sporocysts, due to the effects of exposure to Bge cell-conditioned medium.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182009005733</identifier><identifier>PMID: 19265565</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Biomphalaria - parasitology ; Biomphalaria glabrata ; development ; DNA, Helminth - analysis ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Gene Library ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Helminth Proteins - genetics ; Helminth Proteins - metabolism ; Host-Parasite Interactions ; Invertebrates ; Larva - genetics ; Larva - growth & development ; Larva - metabolism ; Larval development ; Medically important nuisances and vectors, pests of stored products and materials: population survey and control ; miracidia ; Molecular Sequence Data ; Oocysts - growth & development ; Oocysts - metabolism ; SAGE ; Schistosoma mansoni ; Schistosoma mansoni - genetics ; Schistosoma mansoni - growth & development ; Schistosoma mansoni - metabolism ; Sequence Analysis, DNA ; sporocyst ; Swimming ; Trematoda ; Vectors. Intermediate hosts</subject><ispartof>Parasitology, 2009-04, Vol.136 (5), p.469-485</ispartof><rights>Copyright © 2009 Cambridge University Press</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c524t-12f0c1ec594faf05f66fc0c45fe457045d6925f24c6479163907bc936889eefd3</citedby><cites>FETCH-LOGICAL-c524t-12f0c1ec594faf05f66fc0c45fe457045d6925f24c6479163907bc936889eefd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182009005733/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,230,315,781,785,886,27929,27930,55633</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21344705$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19265565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TAFT, A. S.</creatorcontrib><creatorcontrib>VERMEIRE, J. J.</creatorcontrib><creatorcontrib>BERNIER, J.</creatorcontrib><creatorcontrib>BIRKELAND, S. R.</creatorcontrib><creatorcontrib>CIPRIANO, M. J.</creatorcontrib><creatorcontrib>PAPA, A. R.</creatorcontrib><creatorcontrib>McARTHUR, A. G.</creatorcontrib><creatorcontrib>YOSHINO, T. P.</creatorcontrib><title>Transcriptome analysis of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE)</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Infection of the snail, Biomphalaria glabrata, by the free-swimming miracidial stage of the human blood fluke, Schistosoma mansoni, and its subsequent development to the parasitic sporocyst stage is critical to establishment of viable infections and continued human transmission. We performed a genome-wide expression analysis of the S. mansoni miracidia and developing sporocyst using Long Serial Analysis of Gene Expression (LongSAGE). Five cDNA libraries were constructed from miracidia and in vitro cultured 6- and 20-day-old sporocysts maintained in sporocyst medium (SM) or in SM conditioned by previous cultivation with cells of the B. glabrata embryonic (Bge) cell line. We generated 21 440 SAGE tags and mapped 13 381 to the S. mansoni gene predictions (v4.0e) either by estimating theoretical 3′ UTR lengths or using existing 3′ EST sequence data. Overall, 432 transcripts were found to be differentially expressed amongst all 5 libraries. In total, 172 tags were differentially expressed between miracidia and 6-day conditioned sporocysts and 152 were differentially expressed between miracidia and 6-day unconditioned sporocysts. In addition, 53 and 45 tags, respectively, were differentially expressed in 6-day and 20-day cultured sporocysts, due to the effects of exposure to Bge cell-conditioned medium.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biomphalaria - parasitology</subject><subject>Biomphalaria glabrata</subject><subject>development</subject><subject>DNA, Helminth - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Library</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Helminth Proteins - genetics</subject><subject>Helminth Proteins - metabolism</subject><subject>Host-Parasite Interactions</subject><subject>Invertebrates</subject><subject>Larva - genetics</subject><subject>Larva - growth & development</subject><subject>Larva - metabolism</subject><subject>Larval development</subject><subject>Medically important nuisances and vectors, pests of stored products and materials: population survey and control</subject><subject>miracidia</subject><subject>Molecular Sequence Data</subject><subject>Oocysts - growth & development</subject><subject>Oocysts - metabolism</subject><subject>SAGE</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - genetics</subject><subject>Schistosoma mansoni - growth & development</subject><subject>Schistosoma mansoni - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>sporocyst</subject><subject>Swimming</subject><subject>Trematoda</subject><subject>Vectors. 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S. ; VERMEIRE, J. J. ; BERNIER, J. ; BIRKELAND, S. R. ; CIPRIANO, M. J. ; PAPA, A. R. ; McARTHUR, A. G. ; YOSHINO, T. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c524t-12f0c1ec594faf05f66fc0c45fe457045d6925f24c6479163907bc936889eefd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biomphalaria - parasitology</topic><topic>Biomphalaria glabrata</topic><topic>development</topic><topic>DNA, Helminth - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Library</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. 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S.</au><au>VERMEIRE, J. J.</au><au>BERNIER, J.</au><au>BIRKELAND, S. R.</au><au>CIPRIANO, M. J.</au><au>PAPA, A. R.</au><au>McARTHUR, A. G.</au><au>YOSHINO, T. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome analysis of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE)</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>136</volume><issue>5</issue><spage>469</spage><epage>485</epage><pages>469-485</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>Infection of the snail, Biomphalaria glabrata, by the free-swimming miracidial stage of the human blood fluke, Schistosoma mansoni, and its subsequent development to the parasitic sporocyst stage is critical to establishment of viable infections and continued human transmission. We performed a genome-wide expression analysis of the S. mansoni miracidia and developing sporocyst using Long Serial Analysis of Gene Expression (LongSAGE). Five cDNA libraries were constructed from miracidia and in vitro cultured 6- and 20-day-old sporocysts maintained in sporocyst medium (SM) or in SM conditioned by previous cultivation with cells of the B. glabrata embryonic (Bge) cell line. We generated 21 440 SAGE tags and mapped 13 381 to the S. mansoni gene predictions (v4.0e) either by estimating theoretical 3′ UTR lengths or using existing 3′ EST sequence data. Overall, 432 transcripts were found to be differentially expressed amongst all 5 libraries. In total, 172 tags were differentially expressed between miracidia and 6-day conditioned sporocysts and 152 were differentially expressed between miracidia and 6-day unconditioned sporocysts. In addition, 53 and 45 tags, respectively, were differentially expressed in 6-day and 20-day cultured sporocysts, due to the effects of exposure to Bge cell-conditioned medium.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>19265565</pmid><doi>10.1017/S0031182009005733</doi><tpages>17</tpages></addata></record> |
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| ispartof | Parasitology, 2009-04, Vol.136 (5), p.469-485 |
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| subjects | Animals Base Sequence Biological and medical sciences Biomphalaria - parasitology Biomphalaria glabrata development DNA, Helminth - analysis Fundamental and applied biological sciences. Psychology Gene Expression Profiling Gene Expression Regulation, Developmental Gene Library General aspects General aspects and techniques. Study of several systematic groups. Models Helminth Proteins - genetics Helminth Proteins - metabolism Host-Parasite Interactions Invertebrates Larva - genetics Larva - growth & development Larva - metabolism Larval development Medically important nuisances and vectors, pests of stored products and materials: population survey and control miracidia Molecular Sequence Data Oocysts - growth & development Oocysts - metabolism SAGE Schistosoma mansoni Schistosoma mansoni - genetics Schistosoma mansoni - growth & development Schistosoma mansoni - metabolism Sequence Analysis, DNA sporocyst Swimming Trematoda Vectors. Intermediate hosts |
| title | Transcriptome analysis of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE) |
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