Expression of tumor related genes NGX6, NAG-7, BRD7 in gastric and colorectal cancer
AIM: NGX6, NAG-7 and BRD7 genes are tumor related genes, which have been newly cloned by positional candidate cloning strategy. This study was designed to investigate the expression levels of NGX6, NAG-7 and BRD7 genes in human gastric and colorectal cancer tissues, and their corresponding normal ti...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2003-08, Vol.9 (8), p.1729-1733 |
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| description | AIM: NGX6, NAG-7 and BRD7 genes are tumor related genes, which have been newly cloned by positional candidate cloning strategy. This study was designed to investigate the expression levels of NGX6, NAG-7 and BRD7 genes in human gastric and colorectal cancer tissues, and their corresponding normal tissues, and to investigate whether these genes play a role in the pathogenesis of gastric and colorectal cancers.METHODS: Reverse transcription-polymerase chain reaction (RT-PCR), dot hybridization and Northem blot analysis were used to compare the expression levels of NGX6, NAG-7 and BRD7 genes in 34 gastric cancer tissues and 34 colorectal cancer tissues with their corresponding normal tissues of the same patients, respectively.RESULTS: Among the 34 colorectal cancer specimens and the 34 gastric cancer specimens, the expression of NGX6 in 25 colorectal cancer tissues was absent or very weak (73.5 %) by RT-PCR analysis. The down-regulation rate of NGX6 in colorectal cancer tissues was significantly higher than that in corresponding normal tissues (26.5 %,9/34)(P |
| doi_str_mv | 10.3748/wjg.v9.i8.1729 |
| format | Article |
| fullrecord | <record><control><sourceid>wanfang_jour_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4611532</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>8407187</cqvip_id><wanfj_id>wjg200308016</wanfj_id><sourcerecordid>wjg200308016</sourcerecordid><originalsourceid>FETCH-LOGICAL-c441t-f95ba12a7d43fd910013afdd730231f9166122f0f48ec0838ad8d9c4e65799003</originalsourceid><addsrcrecordid>eNpVkc1vEzEQxS0EoqFw5YiMxLG7eOzN2r4glVICUlUkVCRuluOPrcPGTu1N2v73OErEx2kO85v3nuYh9BpIy3gn3t-vhnYn2yBa4FQ-QTNKQTZUdOQpmgEhvJGM8hP0opQVIZSxOX2OToBKEEDkDN1cPmyyKyWkiJPH03adMs5u1JOzeHDRFXy9-Nmf4evzRcPP8MfvnzgOEQ-6TDkYrKPFJo0pOzPpERsdjcsv0TOvx-JeHecp-vH58ubiS3P1bfH14vyqMV0HU-PlfKmBam475q2saYFpby1nNSh4CX0PlHriO-EMEUxoK6w0nevnXEpC2Cn6cNDdbJdrZ42LU9aj2uSw1vlRJR3U_5sYbtWQdqrrAeaMVoF3B4F7Hb2Og1qlbY41sqp_pdWBCAJ9xdoDZnIqJTv_xwKI2tewx9VOqiDUvoZ68ObfYH_x498r8PaoeJvicBeq9VKbXz6MTtXuOAjOfgPs6I4Z</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Expression of tumor related genes NGX6, NAG-7, BRD7 in gastric and colorectal cancer</title><source>PubMed (Medline)</source><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Zhang, Xiao-Mei ; Wang, Xiao-Yan ; Sheng, Shou-Rong ; Wang, Jie-Ru ; Li, Jiang</creator><creatorcontrib>Zhang, Xiao-Mei ; Wang, Xiao-Yan ; Sheng, Shou-Rong ; Wang, Jie-Ru ; Li, Jiang</creatorcontrib><description>AIM: NGX6, NAG-7 and BRD7 genes are tumor related genes, which have been newly cloned by positional candidate cloning strategy. This study was designed to investigate the expression levels of NGX6, NAG-7 and BRD7 genes in human gastric and colorectal cancer tissues, and their corresponding normal tissues, and to investigate whether these genes play a role in the pathogenesis of gastric and colorectal cancers.METHODS: Reverse transcription-polymerase chain reaction (RT-PCR), dot hybridization and Northem blot analysis were used to compare the expression levels of NGX6, NAG-7 and BRD7 genes in 34 gastric cancer tissues and 34 colorectal cancer tissues with their corresponding normal tissues of the same patients, respectively.RESULTS: Among the 34 colorectal cancer specimens and the 34 gastric cancer specimens, the expression of NGX6 in 25 colorectal cancer tissues was absent or very weak (73.5 %) by RT-PCR analysis. The down-regulation rate of NGX6 in colorectal cancer tissues was significantly higher than that in corresponding normal tissues (26.5 %,9/34)(P<0.005). Moreover, the down-regulation of NGX6 was significantly correlated with lymph node and/or distance metastases. Patients with lymph node and/or distance metastasis had much higher down-regulation rate of NGX6 than palJents without metastases (93.8 % vs55.6 %, P<0.05).However no correlation was found between the expression of NGX6 and pathologic type of colorectal cancer in this study, and also the expression of NGX6 did not display any difference between gastric cancer and corresponding normal tissues (58.8 % vs70.6 %, P>0.25). Dot hybridization and Northern blot analysis confirmed the results of RT-PCR.Furthermore, NAG-7 and BRD7 mRNA was not up- or down-regulated in gastric and colorectal cancers compared with their corresponding normal tissues in our study.CONCLUSION: The down-regula'don of NGX6 may be dosely associated with tumorigenesis and metastasis of colorectal cardnoma. However, it may not oontribute to the development and progression of gastric carcinoma. In addition, the expression levels of NAG-7, and BRD7 did not alber in gastric and colorectal cancers. This seems to suggest that NAG-7and BRD7 genes may not play a role in gastric and colorectal carcinogenesis.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v9.i8.1729</identifier><identifier>PMID: 12918109</identifier><language>eng</language><publisher>United States: Department of Digestion Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China %Department of Digestion Medicine, The Third Xiangya Hospital, Central South University, Changsha 410013,Hunan Province, China %Carcinoma Research Institute, Xiangya Medical School, Central South University, Changsha 410078, Hunan Province, China</publisher><subject>Blotting, Northern ; BRD7 ; Cell Cycle Proteins ; Chromosomal Proteins, Non-Histone - genetics ; Colorectal Cancer ; Colorectal Neoplasms - genetics ; Gene Expression ; Humans ; Membrane Proteins - genetics ; NAG-7 ; NGX6 ; Nuclear Proteins ; Nucleic Acid Hybridization - methods ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Long Noncoding ; RNA, Untranslated ; Stomach Neoplasms - genetics ; Tumor Suppressor Proteins - genetics ; 结肠直肠癌 ; 肿瘤相关基因 ; 胃癌</subject><ispartof>World journal of gastroenterology : WJG, 2003-08, Vol.9 (8), p.1729-1733</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved. 2003</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-f95ba12a7d43fd910013afdd730231f9166122f0f48ec0838ad8d9c4e65799003</citedby><cites>FETCH-LOGICAL-c441t-f95ba12a7d43fd910013afdd730231f9166122f0f48ec0838ad8d9c4e65799003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4611532/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4611532/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12918109$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Xiao-Mei</creatorcontrib><creatorcontrib>Wang, Xiao-Yan</creatorcontrib><creatorcontrib>Sheng, Shou-Rong</creatorcontrib><creatorcontrib>Wang, Jie-Ru</creatorcontrib><creatorcontrib>Li, Jiang</creatorcontrib><title>Expression of tumor related genes NGX6, NAG-7, BRD7 in gastric and colorectal cancer</title><title>World journal of gastroenterology : WJG</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM: NGX6, NAG-7 and BRD7 genes are tumor related genes, which have been newly cloned by positional candidate cloning strategy. This study was designed to investigate the expression levels of NGX6, NAG-7 and BRD7 genes in human gastric and colorectal cancer tissues, and their corresponding normal tissues, and to investigate whether these genes play a role in the pathogenesis of gastric and colorectal cancers.METHODS: Reverse transcription-polymerase chain reaction (RT-PCR), dot hybridization and Northem blot analysis were used to compare the expression levels of NGX6, NAG-7 and BRD7 genes in 34 gastric cancer tissues and 34 colorectal cancer tissues with their corresponding normal tissues of the same patients, respectively.RESULTS: Among the 34 colorectal cancer specimens and the 34 gastric cancer specimens, the expression of NGX6 in 25 colorectal cancer tissues was absent or very weak (73.5 %) by RT-PCR analysis. The down-regulation rate of NGX6 in colorectal cancer tissues was significantly higher than that in corresponding normal tissues (26.5 %,9/34)(P<0.005). Moreover, the down-regulation of NGX6 was significantly correlated with lymph node and/or distance metastases. Patients with lymph node and/or distance metastasis had much higher down-regulation rate of NGX6 than palJents without metastases (93.8 % vs55.6 %, P<0.05).However no correlation was found between the expression of NGX6 and pathologic type of colorectal cancer in this study, and also the expression of NGX6 did not display any difference between gastric cancer and corresponding normal tissues (58.8 % vs70.6 %, P>0.25). Dot hybridization and Northern blot analysis confirmed the results of RT-PCR.Furthermore, NAG-7 and BRD7 mRNA was not up- or down-regulated in gastric and colorectal cancers compared with their corresponding normal tissues in our study.CONCLUSION: The down-regula'don of NGX6 may be dosely associated with tumorigenesis and metastasis of colorectal cardnoma. However, it may not oontribute to the development and progression of gastric carcinoma. In addition, the expression levels of NAG-7, and BRD7 did not alber in gastric and colorectal cancers. This seems to suggest that NAG-7and BRD7 genes may not play a role in gastric and colorectal carcinogenesis.</description><subject>Blotting, Northern</subject><subject>BRD7</subject><subject>Cell Cycle Proteins</subject><subject>Chromosomal Proteins, Non-Histone - genetics</subject><subject>Colorectal Cancer</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Membrane Proteins - genetics</subject><subject>NAG-7</subject><subject>NGX6</subject><subject>Nuclear Proteins</subject><subject>Nucleic Acid Hybridization - methods</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Long Noncoding</subject><subject>RNA, Untranslated</subject><subject>Stomach Neoplasms - genetics</subject><subject>Tumor Suppressor Proteins - genetics</subject><subject>结肠直肠癌</subject><subject>肿瘤相关基因</subject><subject>胃癌</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1vEzEQxS0EoqFw5YiMxLG7eOzN2r4glVICUlUkVCRuluOPrcPGTu1N2v73OErEx2kO85v3nuYh9BpIy3gn3t-vhnYn2yBa4FQ-QTNKQTZUdOQpmgEhvJGM8hP0opQVIZSxOX2OToBKEEDkDN1cPmyyKyWkiJPH03adMs5u1JOzeHDRFXy9-Nmf4evzRcPP8MfvnzgOEQ-6TDkYrKPFJo0pOzPpERsdjcsv0TOvx-JeHecp-vH58ubiS3P1bfH14vyqMV0HU-PlfKmBam475q2saYFpby1nNSh4CX0PlHriO-EMEUxoK6w0nevnXEpC2Cn6cNDdbJdrZ42LU9aj2uSw1vlRJR3U_5sYbtWQdqrrAeaMVoF3B4F7Hb2Og1qlbY41sqp_pdWBCAJ9xdoDZnIqJTv_xwKI2tewx9VOqiDUvoZ68ObfYH_x498r8PaoeJvicBeq9VKbXz6MTtXuOAjOfgPs6I4Z</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Zhang, Xiao-Mei</creator><creator>Wang, Xiao-Yan</creator><creator>Sheng, Shou-Rong</creator><creator>Wang, Jie-Ru</creator><creator>Li, Jiang</creator><general>Department of Digestion Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China %Department of Digestion Medicine, The Third Xiangya Hospital, Central South University, Changsha 410013,Hunan Province, China %Carcinoma Research Institute, Xiangya Medical School, Central South University, Changsha 410078, Hunan Province, China</general><general>Baishideng Publishing Group Inc</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20030801</creationdate><title>Expression of tumor related genes NGX6, NAG-7, BRD7 in gastric and colorectal cancer</title><author>Zhang, Xiao-Mei ; Wang, Xiao-Yan ; Sheng, Shou-Rong ; Wang, Jie-Ru ; Li, Jiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-f95ba12a7d43fd910013afdd730231f9166122f0f48ec0838ad8d9c4e65799003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Blotting, Northern</topic><topic>BRD7</topic><topic>Cell Cycle Proteins</topic><topic>Chromosomal Proteins, Non-Histone - genetics</topic><topic>Colorectal Cancer</topic><topic>Colorectal Neoplasms - genetics</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Membrane Proteins - genetics</topic><topic>NAG-7</topic><topic>NGX6</topic><topic>Nuclear Proteins</topic><topic>Nucleic Acid Hybridization - methods</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Long Noncoding</topic><topic>RNA, Untranslated</topic><topic>Stomach Neoplasms - genetics</topic><topic>Tumor Suppressor Proteins - genetics</topic><topic>结肠直肠癌</topic><topic>肿瘤相关基因</topic><topic>胃癌</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xiao-Mei</creatorcontrib><creatorcontrib>Wang, Xiao-Yan</creatorcontrib><creatorcontrib>Sheng, Shou-Rong</creatorcontrib><creatorcontrib>Wang, Jie-Ru</creatorcontrib><creatorcontrib>Li, Jiang</creatorcontrib><collection>维普_期刊</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>维普中文期刊数据库</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Xiao-Mei</au><au>Wang, Xiao-Yan</au><au>Sheng, Shou-Rong</au><au>Wang, Jie-Ru</au><au>Li, Jiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of tumor related genes NGX6, NAG-7, BRD7 in gastric and colorectal cancer</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World Journal of Gastroenterology</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>9</volume><issue>8</issue><spage>1729</spage><epage>1733</epage><pages>1729-1733</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>AIM: NGX6, NAG-7 and BRD7 genes are tumor related genes, which have been newly cloned by positional candidate cloning strategy. This study was designed to investigate the expression levels of NGX6, NAG-7 and BRD7 genes in human gastric and colorectal cancer tissues, and their corresponding normal tissues, and to investigate whether these genes play a role in the pathogenesis of gastric and colorectal cancers.METHODS: Reverse transcription-polymerase chain reaction (RT-PCR), dot hybridization and Northem blot analysis were used to compare the expression levels of NGX6, NAG-7 and BRD7 genes in 34 gastric cancer tissues and 34 colorectal cancer tissues with their corresponding normal tissues of the same patients, respectively.RESULTS: Among the 34 colorectal cancer specimens and the 34 gastric cancer specimens, the expression of NGX6 in 25 colorectal cancer tissues was absent or very weak (73.5 %) by RT-PCR analysis. The down-regulation rate of NGX6 in colorectal cancer tissues was significantly higher than that in corresponding normal tissues (26.5 %,9/34)(P<0.005). Moreover, the down-regulation of NGX6 was significantly correlated with lymph node and/or distance metastases. Patients with lymph node and/or distance metastasis had much higher down-regulation rate of NGX6 than palJents without metastases (93.8 % vs55.6 %, P<0.05).However no correlation was found between the expression of NGX6 and pathologic type of colorectal cancer in this study, and also the expression of NGX6 did not display any difference between gastric cancer and corresponding normal tissues (58.8 % vs70.6 %, P>0.25). Dot hybridization and Northern blot analysis confirmed the results of RT-PCR.Furthermore, NAG-7 and BRD7 mRNA was not up- or down-regulated in gastric and colorectal cancers compared with their corresponding normal tissues in our study.CONCLUSION: The down-regula'don of NGX6 may be dosely associated with tumorigenesis and metastasis of colorectal cardnoma. However, it may not oontribute to the development and progression of gastric carcinoma. In addition, the expression levels of NAG-7, and BRD7 did not alber in gastric and colorectal cancers. This seems to suggest that NAG-7and BRD7 genes may not play a role in gastric and colorectal carcinogenesis.</abstract><cop>United States</cop><pub>Department of Digestion Medicine, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China %Department of Digestion Medicine, The Third Xiangya Hospital, Central South University, Changsha 410013,Hunan Province, China %Carcinoma Research Institute, Xiangya Medical School, Central South University, Changsha 410078, Hunan Province, China</pub><pmid>12918109</pmid><doi>10.3748/wjg.v9.i8.1729</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Blotting, Northern BRD7 Cell Cycle Proteins Chromosomal Proteins, Non-Histone - genetics Colorectal Cancer Colorectal Neoplasms - genetics Gene Expression Humans Membrane Proteins - genetics NAG-7 NGX6 Nuclear Proteins Nucleic Acid Hybridization - methods Reverse Transcriptase Polymerase Chain Reaction RNA, Long Noncoding RNA, Untranslated Stomach Neoplasms - genetics Tumor Suppressor Proteins - genetics 结肠直肠癌 肿瘤相关基因 胃癌 |
| title | Expression of tumor related genes NGX6, NAG-7, BRD7 in gastric and colorectal cancer |
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