A dark green fluorescent protein as an acceptor for measurement of Förster resonance energy transfer

Measurement of Förster resonance energy transfer by fluorescence lifetime imaging microscopy (FLIM-FRET) is a powerful method for visualization of intracellular signaling activities such as protein-protein interactions and conformational changes of proteins. Here, we developed a dark green fluoresce...

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Veröffentlicht in:	Scientific reports 2015-10, Vol.5 (1), p.15334, Article 15334
Hauptverfasser:	Murakoshi, Hideji, Shibata, Akihiro C. E., Nakahata, Yoshihisa, Nabekura, Junichi
Format:	Artikel
Sprache:	eng
Schlagworte:	631/57/2267 631/80/2373 96 96/35 96/63 Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - metabolism Ca2+/calmodulin-dependent protein kinase II Calcium-Calmodulin-Dependent Protein Kinase Type 2 - chemistry Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism Energy transfer Fluorescence Fluorescence Resonance Energy Transfer Green fluorescent protein Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism HEK293 Cells HeLa Cells Humanities and Social Sciences Humans Intracellular signalling Luminescent Proteins - chemistry Luminescent Proteins - genetics Luminescent Proteins - metabolism Microscopy, Fluorescence multidisciplinary Mutagenesis Plasmids - metabolism Protein folding Protein interaction Protein Interaction Domains and Motifs Proteins Quantum Theory ras Proteins - chemistry ras Proteins - genetics ras Proteins - metabolism Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Red Fluorescent Protein Resonance Science Sensors Translocation
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