Image restoration for three-dimensional fluorescence microscopy using an orthonormal basis for efficient representation of depth-variant point-spread functions

A depth-variant (DV) image restoration algorithm for wide field fluorescence microscopy, using an orthonormal basis decomposition of DV point-spread functions (PSFs), is investigated in this study. The efficient PSF representation is based on a previously developed principal component analysis (PCA)...

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Veröffentlicht in:Biomedical optics express 2015-10, Vol.6 (10), p.3826-3841
Hauptverfasser: Patwary, Nurmohammed, Preza, Chrysanthe
Format: Artikel
Sprache:eng
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