Three-dimensional super-resolution protein localization correlated with vitrified cellular context

We demonstrate the use of cryogenic super-resolution correlative light and electron microscopy (csCLEM) to precisely determine the spatial relationship between proteins and their native cellular structures. Several fluorescent proteins (FPs) were found to be photoswitchable and emitted far more phot...

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Veröffentlicht in:	Scientific reports 2015-10, Vol.5 (1), p.13017-13017, Article 13017
Hauptverfasser:	Liu, Bei, Xue, Yanhong, Zhao, Wei, Chen, Yan, Fan, Chunyan, Gu, Lusheng, Zhang, Yongdeng, Zhang, Xiang, Sun, Lei, Huang, Xiaojun, Ding, Wei, Sun, Fei, Ji, Wei, Xu, Tao
Format:	Artikel
Sprache:	eng
Schlagworte:	631/57/2265 631/57/2282 Cryopreservation - instrumentation Cryopreservation - methods Electron microscopy Equipment Design Equipment Failure Analysis Freezing HEK293 Cells High pressure Humanities and Social Sciences Humans Image Enhancement - instrumentation Image Enhancement - methods Imaging, Three-Dimensional - instrumentation Imaging, Three-Dimensional - methods Localization Mammalian cells Micro-Electrical-Mechanical Systems - instrumentation Micro-Electrical-Mechanical Systems - methods Microscopy, Electron - instrumentation Microscopy, Fluorescence - instrumentation Microscopy, Fluorescence - methods Mitochondria Molecular Imaging - instrumentation Molecular Imaging - methods multidisciplinary Multimodal Imaging - instrumentation Multimodal Imaging - methods Preservation Proteins - metabolism Reproducibility of Results Science Sectioning Sensitivity and Specificity Statistics as Topic Subcellular Fractions - metabolism Tissue Distribution Vitrification
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