Continuous directed evolution of DNA-binding proteins to improve TALEN specificity

DNA-binding phage-assisted continuous evolution improves the specificity of DNA-binding proteins, as demonstrated with TALENs. Nucleases containing programmable DNA-binding domains can alter the genomes of model organisms and have the potential to become human therapeutics. Here we present DNA-bindi...

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Veröffentlicht in:	Nature methods 2015-10, Vol.12 (10), p.939-942
Hauptverfasser:	Hubbard, Basil P, Badran, Ahmed H, Zuris, John A, Guilinger, John P, Davis, Kevin M, Chen, Liwei, Tsai, Shengdar Q, Sander, Jeffry D, Joung, J Keith, Liu, David R
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1511 631/1647/1513/1967 631/1647/2163 631/181 Binding sites Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Deoxyribonucleases - metabolism Deoxyribonucleic acid Directed Molecular Evolution - methods DNA DNA binding proteins DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Gene Targeting - methods Genetic engineering Genomes High-Throughput Screening Assays - methods Humans Life Sciences Methods Nucleases Observations Polycomb Repressive Complex 1 - genetics Polycomb Repressive Complex 1 - metabolism Properties Protein Engineering - methods Proteins Proteomics Scission (Chemistry) Transcription factors
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