Fumonisins affect the intestinal microbial homeostasis in broiler chickens, predisposing to necrotic enteritis
Fumonisins (FBs) are mycotoxins produced by Fusarium fungi. This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided in...
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creator | Antonissen, Gunther Croubels, Siska Pasmans, Frank Ducatelle, Richard Eeckhaut, Venessa Devreese, Mathias Verlinden, Marc Haesebrouck, Freddy Eeckhout, Mia De Saeger, Sarah Antlinger, Birgit Novak, Barbara Martel, An Van Immerseel, Filip |
description | Fumonisins (FBs) are mycotoxins produced by Fusarium fungi. This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 ± 0.016) compared to the control (0.14 ± 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 ± 0.30 versus 6.3 ± 0.24 log10 copies/g intestinal content). After C. perfringens challenge, a higher percentage of birds developed subclinical necrotic enteritis in the group fed a FBs contaminated diet as compared to the control (44.9 ± 2.22% versus 29.8 ± 5.46%). |
doi_str_mv | 10.1186/s13567-015-0234-8 |
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This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 ± 0.016) compared to the control (0.14 ± 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 ± 0.30 versus 6.3 ± 0.24 log10 copies/g intestinal content). After C. perfringens challenge, a higher percentage of birds developed subclinical necrotic enteritis in the group fed a FBs contaminated diet as compared to the control (44.9 ± 2.22% versus 29.8 ± 5.46%).</description><identifier>ISSN: 1297-9716</identifier><identifier>ISSN: 0928-4249</identifier><identifier>EISSN: 1297-9716</identifier><identifier>DOI: 10.1186/s13567-015-0234-8</identifier><identifier>PMID: 26394675</identifier><language>eng</language><publisher>England: BioMed Central</publisher><subject>Animal Feed - microbiology ; Animals ; Asymptomatic Infections ; biosynthesis ; broiler chickens ; Chickens ; Clostridium Infections - microbiology ; Clostridium Infections - veterinary ; Clostridium perfringens ; Clostridium perfringens - physiology ; denaturing gradient gel electrophoresis ; Denaturing Gradient Gel Electrophoresis - veterinary ; diet ; Diet - veterinary ; digesta ; Dose-Response Relationship, Drug ; Enteritis - microbiology ; Enteritis - veterinary ; feed contamination ; Food Microbiology ; fumonisins ; Fumonisins - toxicity ; fungi ; Fusarium ; Fusarium - chemistry ; Gastrointestinal Microbiome - drug effects ; genes ; homeostasis ; Homeostasis - drug effects ; ileum ; Intestines - anatomy & histology ; Intestines - drug effects ; Intestines - microbiology ; Life Sciences ; Medical research ; Mycotoxins - toxicity ; Necrosis - microbiology ; Necrosis - veterinary ; necrotic enteritis ; Poultry Diseases - microbiology ; quantitative polymerase chain reaction ; sphingolipids ; sphingosine ; Veterinary medicine ; villi</subject><ispartof>Veterinary research (Paris), 2015-09, Vol.46 (1), p.98-98, Article 98</ispartof><rights>Copyright BioMed Central 2015</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Antonissen et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-433cb908ab35b82b4f501d8ad7c5b45da2c7441a5d99bc69e64fd22ae123e9aa3</citedby><cites>FETCH-LOGICAL-c494t-433cb908ab35b82b4f501d8ad7c5b45da2c7441a5d99bc69e64fd22ae123e9aa3</cites><orcidid>0000-0003-0455-3350</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579638/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4579638/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26394675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01341410$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Antonissen, Gunther</creatorcontrib><creatorcontrib>Croubels, Siska</creatorcontrib><creatorcontrib>Pasmans, Frank</creatorcontrib><creatorcontrib>Ducatelle, Richard</creatorcontrib><creatorcontrib>Eeckhaut, Venessa</creatorcontrib><creatorcontrib>Devreese, Mathias</creatorcontrib><creatorcontrib>Verlinden, Marc</creatorcontrib><creatorcontrib>Haesebrouck, Freddy</creatorcontrib><creatorcontrib>Eeckhout, Mia</creatorcontrib><creatorcontrib>De Saeger, Sarah</creatorcontrib><creatorcontrib>Antlinger, Birgit</creatorcontrib><creatorcontrib>Novak, Barbara</creatorcontrib><creatorcontrib>Martel, An</creatorcontrib><creatorcontrib>Van Immerseel, Filip</creatorcontrib><title>Fumonisins affect the intestinal microbial homeostasis in broiler chickens, predisposing to necrotic enteritis</title><title>Veterinary research (Paris)</title><addtitle>Vet Res</addtitle><description>Fumonisins (FBs) are mycotoxins produced by Fusarium fungi. This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 ± 0.016) compared to the control (0.14 ± 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 ± 0.30 versus 6.3 ± 0.24 log10 copies/g intestinal content). After C. perfringens challenge, a higher percentage of birds developed subclinical necrotic enteritis in the group fed a FBs contaminated diet as compared to the control (44.9 ± 2.22% versus 29.8 ± 5.46%).</description><subject>Animal Feed - microbiology</subject><subject>Animals</subject><subject>Asymptomatic Infections</subject><subject>biosynthesis</subject><subject>broiler chickens</subject><subject>Chickens</subject><subject>Clostridium Infections - microbiology</subject><subject>Clostridium Infections - veterinary</subject><subject>Clostridium perfringens</subject><subject>Clostridium perfringens - physiology</subject><subject>denaturing gradient gel electrophoresis</subject><subject>Denaturing Gradient Gel Electrophoresis - veterinary</subject><subject>diet</subject><subject>Diet - veterinary</subject><subject>digesta</subject><subject>Dose-Response Relationship, Drug</subject><subject>Enteritis - microbiology</subject><subject>Enteritis - veterinary</subject><subject>feed contamination</subject><subject>Food Microbiology</subject><subject>fumonisins</subject><subject>Fumonisins - toxicity</subject><subject>fungi</subject><subject>Fusarium</subject><subject>Fusarium - chemistry</subject><subject>Gastrointestinal Microbiome - drug effects</subject><subject>genes</subject><subject>homeostasis</subject><subject>Homeostasis - drug effects</subject><subject>ileum</subject><subject>Intestines - anatomy & histology</subject><subject>Intestines - drug effects</subject><subject>Intestines - microbiology</subject><subject>Life Sciences</subject><subject>Medical research</subject><subject>Mycotoxins - toxicity</subject><subject>Necrosis - microbiology</subject><subject>Necrosis - veterinary</subject><subject>necrotic enteritis</subject><subject>Poultry Diseases - microbiology</subject><subject>quantitative polymerase chain reaction</subject><subject>sphingolipids</subject><subject>sphingosine</subject><subject>Veterinary medicine</subject><subject>villi</subject><issn>1297-9716</issn><issn>0928-4249</issn><issn>1297-9716</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkU1rFTEYhYMotlZ_gBsJuFFwNG8-JxuhFGuFC250HZJMppM6M7kmmYL_3lxuLbWrhOR5T07OQeg1kI8AvfxUgAmpOgKiI5Txrn-CToFq1WkF8umD_Ql6UcoNISCZ4M_RCZVMc6nEKVovtyWtscS1YDuOwVdcp4DjWkOpcbUzXqLPycW2m9ISUqm2xNIA7HKKc8jYT9H_Cmv5gPc5DLHsU1O7xjXhNbTRGj0OTS7HGstL9Gy0cwmv7tYz9PPyy4-Lq273_eu3i_Nd57nmteOMeadJbx0TrqeOj4LA0NtBeeG4GCz1inOwYtDaeamD5ONAqQ1AWdDWsjP0-ai739wSBt8MZDubfY6LzX9MstH8f7PGyVynW8OF0pL1TeD9UWB6NHZ1vjOHMwKMAwdyC419d_dYTr-3lptZYvFhnu0a0lYMJS15YIqKhr59hN6kLbeYiwGlKOsbxhoFR6rFV0oO470DIObQvDk230wIc2jeHAy_efjj-4l_VbO_i3ur6A</recordid><startdate>20150923</startdate><enddate>20150923</enddate><creator>Antonissen, Gunther</creator><creator>Croubels, Siska</creator><creator>Pasmans, Frank</creator><creator>Ducatelle, Richard</creator><creator>Eeckhaut, Venessa</creator><creator>Devreese, Mathias</creator><creator>Verlinden, Marc</creator><creator>Haesebrouck, Freddy</creator><creator>Eeckhout, Mia</creator><creator>De Saeger, Sarah</creator><creator>Antlinger, Birgit</creator><creator>Novak, Barbara</creator><creator>Martel, An</creator><creator>Van Immerseel, Filip</creator><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7S9</scope><scope>L.6</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0455-3350</orcidid></search><sort><creationdate>20150923</creationdate><title>Fumonisins affect the intestinal microbial homeostasis in broiler chickens, predisposing to necrotic enteritis</title><author>Antonissen, Gunther ; Croubels, Siska ; Pasmans, Frank ; Ducatelle, Richard ; Eeckhaut, Venessa ; Devreese, Mathias ; Verlinden, Marc ; Haesebrouck, Freddy ; Eeckhout, Mia ; De Saeger, Sarah ; Antlinger, Birgit ; Novak, Barbara ; Martel, An ; Van Immerseel, Filip</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-433cb908ab35b82b4f501d8ad7c5b45da2c7441a5d99bc69e64fd22ae123e9aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animal Feed - microbiology</topic><topic>Animals</topic><topic>Asymptomatic Infections</topic><topic>biosynthesis</topic><topic>broiler chickens</topic><topic>Chickens</topic><topic>Clostridium Infections - microbiology</topic><topic>Clostridium Infections - veterinary</topic><topic>Clostridium perfringens</topic><topic>Clostridium perfringens - physiology</topic><topic>denaturing gradient gel electrophoresis</topic><topic>Denaturing Gradient Gel Electrophoresis - veterinary</topic><topic>diet</topic><topic>Diet - veterinary</topic><topic>digesta</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enteritis - microbiology</topic><topic>Enteritis - veterinary</topic><topic>feed contamination</topic><topic>Food Microbiology</topic><topic>fumonisins</topic><topic>Fumonisins - toxicity</topic><topic>fungi</topic><topic>Fusarium</topic><topic>Fusarium - chemistry</topic><topic>Gastrointestinal Microbiome - drug effects</topic><topic>genes</topic><topic>homeostasis</topic><topic>Homeostasis - drug effects</topic><topic>ileum</topic><topic>Intestines - anatomy & histology</topic><topic>Intestines - drug effects</topic><topic>Intestines - microbiology</topic><topic>Life Sciences</topic><topic>Medical research</topic><topic>Mycotoxins - toxicity</topic><topic>Necrosis - microbiology</topic><topic>Necrosis - veterinary</topic><topic>necrotic enteritis</topic><topic>Poultry Diseases - microbiology</topic><topic>quantitative polymerase chain reaction</topic><topic>sphingolipids</topic><topic>sphingosine</topic><topic>Veterinary medicine</topic><topic>villi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Antonissen, Gunther</creatorcontrib><creatorcontrib>Croubels, Siska</creatorcontrib><creatorcontrib>Pasmans, Frank</creatorcontrib><creatorcontrib>Ducatelle, Richard</creatorcontrib><creatorcontrib>Eeckhaut, Venessa</creatorcontrib><creatorcontrib>Devreese, Mathias</creatorcontrib><creatorcontrib>Verlinden, Marc</creatorcontrib><creatorcontrib>Haesebrouck, Freddy</creatorcontrib><creatorcontrib>Eeckhout, Mia</creatorcontrib><creatorcontrib>De Saeger, Sarah</creatorcontrib><creatorcontrib>Antlinger, Birgit</creatorcontrib><creatorcontrib>Novak, Barbara</creatorcontrib><creatorcontrib>Martel, An</creatorcontrib><creatorcontrib>Van Immerseel, Filip</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Veterinary research (Paris)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Antonissen, Gunther</au><au>Croubels, Siska</au><au>Pasmans, Frank</au><au>Ducatelle, Richard</au><au>Eeckhaut, Venessa</au><au>Devreese, Mathias</au><au>Verlinden, Marc</au><au>Haesebrouck, Freddy</au><au>Eeckhout, Mia</au><au>De Saeger, Sarah</au><au>Antlinger, Birgit</au><au>Novak, Barbara</au><au>Martel, An</au><au>Van Immerseel, Filip</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fumonisins affect the intestinal microbial homeostasis in broiler chickens, predisposing to necrotic enteritis</atitle><jtitle>Veterinary research (Paris)</jtitle><addtitle>Vet Res</addtitle><date>2015-09-23</date><risdate>2015</risdate><volume>46</volume><issue>1</issue><spage>98</spage><epage>98</epage><pages>98-98</pages><artnum>98</artnum><issn>1297-9716</issn><issn>0928-4249</issn><eissn>1297-9716</eissn><abstract>Fumonisins (FBs) are mycotoxins produced by Fusarium fungi. This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 ± 0.016) compared to the control (0.14 ± 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 ± 0.30 versus 6.3 ± 0.24 log10 copies/g intestinal content). After C. perfringens challenge, a higher percentage of birds developed subclinical necrotic enteritis in the group fed a FBs contaminated diet as compared to the control (44.9 ± 2.22% versus 29.8 ± 5.46%).</abstract><cop>England</cop><pub>BioMed Central</pub><pmid>26394675</pmid><doi>10.1186/s13567-015-0234-8</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-0455-3350</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Animal Feed - microbiology Animals Asymptomatic Infections biosynthesis broiler chickens Chickens Clostridium Infections - microbiology Clostridium Infections - veterinary Clostridium perfringens Clostridium perfringens - physiology denaturing gradient gel electrophoresis Denaturing Gradient Gel Electrophoresis - veterinary diet Diet - veterinary digesta Dose-Response Relationship, Drug Enteritis - microbiology Enteritis - veterinary feed contamination Food Microbiology fumonisins Fumonisins - toxicity fungi Fusarium Fusarium - chemistry Gastrointestinal Microbiome - drug effects genes homeostasis Homeostasis - drug effects ileum Intestines - anatomy & histology Intestines - drug effects Intestines - microbiology Life Sciences Medical research Mycotoxins - toxicity Necrosis - microbiology Necrosis - veterinary necrotic enteritis Poultry Diseases - microbiology quantitative polymerase chain reaction sphingolipids sphingosine Veterinary medicine villi |
title | Fumonisins affect the intestinal microbial homeostasis in broiler chickens, predisposing to necrotic enteritis |
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