Fumonisins affect the intestinal microbial homeostasis in broiler chickens, predisposing to necrotic enteritis

Fumonisins (FBs) are mycotoxins produced by Fusarium fungi. This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided in...

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Veröffentlicht in:Veterinary research (Paris) 2015-09, Vol.46 (1), p.98-98, Article 98
Hauptverfasser: Antonissen, Gunther, Croubels, Siska, Pasmans, Frank, Ducatelle, Richard, Eeckhaut, Venessa, Devreese, Mathias, Verlinden, Marc, Haesebrouck, Freddy, Eeckhout, Mia, De Saeger, Sarah, Antlinger, Birgit, Novak, Barbara, Martel, An, Van Immerseel, Filip
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container_issue 1
container_start_page 98
container_title Veterinary research (Paris)
container_volume 46
creator Antonissen, Gunther
Croubels, Siska
Pasmans, Frank
Ducatelle, Richard
Eeckhaut, Venessa
Devreese, Mathias
Verlinden, Marc
Haesebrouck, Freddy
Eeckhout, Mia
De Saeger, Sarah
Antlinger, Birgit
Novak, Barbara
Martel, An
Van Immerseel, Filip
description Fumonisins (FBs) are mycotoxins produced by Fusarium fungi. This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 ± 0.016) compared to the control (0.14 ± 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 ± 0.30 versus 6.3 ± 0.24 log10 copies/g intestinal content). After C. perfringens challenge, a higher percentage of birds developed subclinical necrotic enteritis in the group fed a FBs contaminated diet as compared to the control (44.9 ± 2.22% versus 29.8 ± 5.46%).
doi_str_mv 10.1186/s13567-015-0234-8
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This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 ± 0.016) compared to the control (0.14 ± 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 ± 0.30 versus 6.3 ± 0.24 log10 copies/g intestinal content). 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A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 ± 0.30 versus 6.3 ± 0.24 log10 copies/g intestinal content). 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Croubels, Siska ; Pasmans, Frank ; Ducatelle, Richard ; Eeckhaut, Venessa ; Devreese, Mathias ; Verlinden, Marc ; Haesebrouck, Freddy ; Eeckhout, Mia ; De Saeger, Sarah ; Antlinger, Birgit ; Novak, Barbara ; Martel, An ; Van Immerseel, Filip</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-433cb908ab35b82b4f501d8ad7c5b45da2c7441a5d99bc69e64fd22ae123e9aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animal Feed - microbiology</topic><topic>Animals</topic><topic>Asymptomatic Infections</topic><topic>biosynthesis</topic><topic>broiler chickens</topic><topic>Chickens</topic><topic>Clostridium Infections - microbiology</topic><topic>Clostridium Infections - veterinary</topic><topic>Clostridium perfringens</topic><topic>Clostridium perfringens - physiology</topic><topic>denaturing gradient gel electrophoresis</topic><topic>Denaturing Gradient Gel Electrophoresis - veterinary</topic><topic>diet</topic><topic>Diet - veterinary</topic><topic>digesta</topic><topic>Dose-Response Relationship, Drug</topic><topic>Enteritis - microbiology</topic><topic>Enteritis - veterinary</topic><topic>feed contamination</topic><topic>Food Microbiology</topic><topic>fumonisins</topic><topic>Fumonisins - toxicity</topic><topic>fungi</topic><topic>Fusarium</topic><topic>Fusarium - chemistry</topic><topic>Gastrointestinal Microbiome - drug effects</topic><topic>genes</topic><topic>homeostasis</topic><topic>Homeostasis - drug effects</topic><topic>ileum</topic><topic>Intestines - anatomy &amp; 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This study aimed to investigate the effect of these feed contaminants on the intestinal morphology and microbiota composition, and to evaluate whether FBs predispose broilers to necrotic enteritis. One-day-old broiler chicks were divided into a group fed a control diet, and a group fed a FBs contaminated diet (18.6 mg FB1+FB2/kg feed). A significant increase in the plasma sphinganine/sphingosine ratio in the FBs-treated group (0.21 ± 0.016) compared to the control (0.14 ± 0.014) indicated disturbance of the sphingolipid biosynthesis. Furthermore, villus height and crypt depth of the ileum was significantly reduced by FBs. Denaturing gradient gel electrophoresis showed a shift in the microbiota composition in the ileum in the FBs group compared to the control. A reduced presence of low-GC containing operational taxonomic units in ileal digesta of birds exposed to FBs was demonstrated, and identified as a reduced abundance of Candidatus Savagella and Lactobaccilus spp. Quantification of total Clostridium perfringens in these ileal samples, previous to experimental infection, using cpa gene (alpha toxin) quantification by qPCR showed an increase in C. perfringens in chickens fed a FBs contaminated diet compared to control (7.5 ± 0.30 versus 6.3 ± 0.24 log10 copies/g intestinal content). After C. perfringens challenge, a higher percentage of birds developed subclinical necrotic enteritis in the group fed a FBs contaminated diet as compared to the control (44.9 ± 2.22% versus 29.8 ± 5.46%).</abstract><cop>England</cop><pub>BioMed Central</pub><pmid>26394675</pmid><doi>10.1186/s13567-015-0234-8</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-0455-3350</orcidid><oa>free_for_read</oa></addata></record>
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subjects Animal Feed - microbiology
Animals
Asymptomatic Infections
biosynthesis
broiler chickens
Chickens
Clostridium Infections - microbiology
Clostridium Infections - veterinary
Clostridium perfringens
Clostridium perfringens - physiology
denaturing gradient gel electrophoresis
Denaturing Gradient Gel Electrophoresis - veterinary
diet
Diet - veterinary
digesta
Dose-Response Relationship, Drug
Enteritis - microbiology
Enteritis - veterinary
feed contamination
Food Microbiology
fumonisins
Fumonisins - toxicity
fungi
Fusarium
Fusarium - chemistry
Gastrointestinal Microbiome - drug effects
genes
homeostasis
Homeostasis - drug effects
ileum
Intestines - anatomy & histology
Intestines - drug effects
Intestines - microbiology
Life Sciences
Medical research
Mycotoxins - toxicity
Necrosis - microbiology
Necrosis - veterinary
necrotic enteritis
Poultry Diseases - microbiology
quantitative polymerase chain reaction
sphingolipids
sphingosine
Veterinary medicine
villi
title Fumonisins affect the intestinal microbial homeostasis in broiler chickens, predisposing to necrotic enteritis
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