CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins

Chromatin immunoprecipitation followed by next-generation DNA sequencing (ChIP-seq) is a widely used technique for identifying transcription factor (TF) binding events throughout an entire genome. However, ChIP-seq is limited by the availability of suitable ChIP-seq grade antibodies, and the vast ma...

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Veröffentlicht in:	Genome research 2015-10, Vol.25 (10), p.1581-1589
Hauptverfasser:	Savic, Daniel, Partridge, E Christopher, Newberry, Kimberly M, Smith, Sophia B, Meadows, Sarah K, Roberts, Brian S, Mackiewicz, Mark, Mendenhall, Eric M, Myers, Richard M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Clustered Regularly Interspaced Short Palindromic Repeats DNA-Binding Proteins - immunology Epitope Mapping - methods Epitopes - analysis Feasibility Studies Gene Expression Profiling Humans Method Mice Oligonucleotide Array Sequence Analysis - methods Transcription Factors - analysis Transcription Factors - immunology Transcriptome Tumor Cells, Cultured
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