Antibacterial Action of Nitric Oxide-Releasing Chitosan Oligosaccharides against Pseudomonas aeruginosa under Aerobic and Anaerobic Conditions
Chitosan oligosaccharides were modified with N-diazeniumdiolates to yield biocompatible nitric oxide (NO) donor scaffolds. The minimum bactericidal concentrations and MICs of the NO donors against Pseudomonas aeruginosa were compared under aerobic and anaerobic conditions. Differential antibacterial...
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| Veröffentlicht in: | Antimicrobial agents and chemotherapy 2015-10, Vol.59 (10), p.6506-6513 |
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| creator | Reighard, Katelyn P Schoenfisch, Mark H |
| description | Chitosan oligosaccharides were modified with N-diazeniumdiolates to yield biocompatible nitric oxide (NO) donor scaffolds. The minimum bactericidal concentrations and MICs of the NO donors against Pseudomonas aeruginosa were compared under aerobic and anaerobic conditions. Differential antibacterial activities were primarily the result of NO scavenging by oxygen under aerobic environments and not changes in bacterial physiology. Bacterial killing was also tested against nonmucoid and mucoid biofilms and compared to that of tobramycin. Smaller NO payloads were required to eradicate P. aeruginosa biofilms under anaerobic versus aerobic conditions. Under oxygen-free environments, the NO treatment was 10-fold more effective at killing biofilms than tobramycin. These results demonstrate the potential utility of NO-releasing chitosan oligosaccharides under both aerobic and anaerobic environments. |
| doi_str_mv | 10.1128/AAC.01208-15 |
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The minimum bactericidal concentrations and MICs of the NO donors against Pseudomonas aeruginosa were compared under aerobic and anaerobic conditions. Differential antibacterial activities were primarily the result of NO scavenging by oxygen under aerobic environments and not changes in bacterial physiology. Bacterial killing was also tested against nonmucoid and mucoid biofilms and compared to that of tobramycin. Smaller NO payloads were required to eradicate P. aeruginosa biofilms under anaerobic versus aerobic conditions. Under oxygen-free environments, the NO treatment was 10-fold more effective at killing biofilms than tobramycin. These results demonstrate the potential utility of NO-releasing chitosan oligosaccharides under both aerobic and anaerobic environments.</description><identifier>ISSN: 0066-4804</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AAC.01208-15</identifier><identifier>PMID: 26239983</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Aerobiosis ; Anaerobiosis ; Anti-Bacterial Agents ; Anti-Bacterial Agents - chemical synthesis ; Anti-Bacterial Agents - pharmacology ; Azo Compounds - chemistry ; Biofilms ; Biofilms - drug effects ; Biofilms - growth & development ; Chitosan ; Chitosan - analogs & derivatives ; Chitosan - chemical synthesis ; Chitosan - pharmacology ; Glycosaminoglycans - biosynthesis ; Mechanisms of Action: Physiological Effects ; Microbial Sensitivity Tests ; Nitric Oxide ; Nitric Oxide - chemistry ; Nitric Oxide - pharmacology ; Nitric Oxide Donors ; Nitric Oxide Donors - chemical synthesis ; Nitric Oxide Donors - pharmacology ; Oxygen - pharmacology ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - growth & development ; Tobramycin - pharmacology</subject><ispartof>Antimicrobial agents and chemotherapy, 2015-10, Vol.59 (10), p.6506-6513</ispartof><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</rights><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved. 2015 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a527t-f437d5b864d740233dfecd382111fbcab9b64e835c5aba4591756ffc19d377753</citedby><cites>FETCH-LOGICAL-a527t-f437d5b864d740233dfecd382111fbcab9b64e835c5aba4591756ffc19d377753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576085/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4576085/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26239983$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reighard, Katelyn P</creatorcontrib><creatorcontrib>Schoenfisch, Mark H</creatorcontrib><title>Antibacterial Action of Nitric Oxide-Releasing Chitosan Oligosaccharides against Pseudomonas aeruginosa under Aerobic and Anaerobic Conditions</title><title>Antimicrobial agents and chemotherapy</title><addtitle>Antimicrob Agents Chemother</addtitle><addtitle>Antimicrob Agents Chemother</addtitle><description>Chitosan oligosaccharides were modified with N-diazeniumdiolates to yield biocompatible nitric oxide (NO) donor scaffolds. The minimum bactericidal concentrations and MICs of the NO donors against Pseudomonas aeruginosa were compared under aerobic and anaerobic conditions. Differential antibacterial activities were primarily the result of NO scavenging by oxygen under aerobic environments and not changes in bacterial physiology. Bacterial killing was also tested against nonmucoid and mucoid biofilms and compared to that of tobramycin. Smaller NO payloads were required to eradicate P. aeruginosa biofilms under anaerobic versus aerobic conditions. Under oxygen-free environments, the NO treatment was 10-fold more effective at killing biofilms than tobramycin. These results demonstrate the potential utility of NO-releasing chitosan oligosaccharides under both aerobic and anaerobic environments.</description><subject>Aerobiosis</subject><subject>Anaerobiosis</subject><subject>Anti-Bacterial Agents</subject><subject>Anti-Bacterial Agents - chemical synthesis</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Azo Compounds - chemistry</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth & development</subject><subject>Chitosan</subject><subject>Chitosan - analogs & derivatives</subject><subject>Chitosan - chemical synthesis</subject><subject>Chitosan - pharmacology</subject><subject>Glycosaminoglycans - biosynthesis</subject><subject>Mechanisms of Action: Physiological Effects</subject><subject>Microbial Sensitivity Tests</subject><subject>Nitric Oxide</subject><subject>Nitric Oxide - chemistry</subject><subject>Nitric Oxide - pharmacology</subject><subject>Nitric Oxide Donors</subject><subject>Nitric Oxide Donors - chemical synthesis</subject><subject>Nitric Oxide Donors - pharmacology</subject><subject>Oxygen - pharmacology</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - growth & development</subject><subject>Tobramycin - pharmacology</subject><issn>0066-4804</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1v1DAQhi0EotvCjTPyESTS-iNO4gtSFEGpVHURgrM1sZ2sq8QudlLBn-A318suFRw42WM_ekYzL0KvKDmnlDUXbdudE8pIU1DxBG0okU1RCVk9RRtCqqooG1KeoNOUbkmuhSTP0QmrGJey4Rv0q_WL60EvNjqYcKsXFzwOA75xS3Qab384Y4svdrKQnB9xt3NLSODxdnJjvmi9g5iRhGEE59OCPye7mjAHD_nNxnV0PnN49cZG3NoY-qwFb3Dr4Vh1wRu3b5xeoGcDTMm-PJ5n6NvHD1-7T8X19vKqa68LEKxeiqHktRF9U5WmLgnj3AxWG94wSunQa-hlX5W24UIL6KEUktaiGgZNpeF1XQt-ht4fvHdrP1ujrV8iTOouuhniTxXAqX9_vNupMdyrUtQVafaCN0dBDN9XmxY1u6TtNIG3YU2K1pTLvHrOMvrugOoYUop2eGxDidpHqHKE6neEiu7Nbw84pJmp27BGnzfxP_b132M8iv_kyx8AbrOnPA</recordid><startdate>20151001</startdate><enddate>20151001</enddate><creator>Reighard, Katelyn P</creator><creator>Schoenfisch, Mark H</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20151001</creationdate><title>Antibacterial Action of Nitric Oxide-Releasing Chitosan Oligosaccharides against Pseudomonas aeruginosa under Aerobic and Anaerobic Conditions</title><author>Reighard, Katelyn P ; Schoenfisch, Mark H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a527t-f437d5b864d740233dfecd382111fbcab9b64e835c5aba4591756ffc19d377753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aerobiosis</topic><topic>Anaerobiosis</topic><topic>Anti-Bacterial Agents</topic><topic>Anti-Bacterial Agents - chemical synthesis</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Azo Compounds - chemistry</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biofilms - growth & development</topic><topic>Chitosan</topic><topic>Chitosan - analogs & derivatives</topic><topic>Chitosan - chemical synthesis</topic><topic>Chitosan - pharmacology</topic><topic>Glycosaminoglycans - biosynthesis</topic><topic>Mechanisms of Action: Physiological Effects</topic><topic>Microbial Sensitivity Tests</topic><topic>Nitric Oxide</topic><topic>Nitric Oxide - chemistry</topic><topic>Nitric Oxide - pharmacology</topic><topic>Nitric Oxide Donors</topic><topic>Nitric Oxide Donors - chemical synthesis</topic><topic>Nitric Oxide Donors - pharmacology</topic><topic>Oxygen - pharmacology</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - growth & development</topic><topic>Tobramycin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reighard, Katelyn P</creatorcontrib><creatorcontrib>Schoenfisch, Mark H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Antimicrobial agents and chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reighard, Katelyn P</au><au>Schoenfisch, Mark H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial Action of Nitric Oxide-Releasing Chitosan Oligosaccharides against Pseudomonas aeruginosa under Aerobic and Anaerobic Conditions</atitle><jtitle>Antimicrobial agents and chemotherapy</jtitle><stitle>Antimicrob Agents Chemother</stitle><addtitle>Antimicrob Agents Chemother</addtitle><date>2015-10-01</date><risdate>2015</risdate><volume>59</volume><issue>10</issue><spage>6506</spage><epage>6513</epage><pages>6506-6513</pages><issn>0066-4804</issn><eissn>1098-6596</eissn><abstract>Chitosan oligosaccharides were modified with N-diazeniumdiolates to yield biocompatible nitric oxide (NO) donor scaffolds. The minimum bactericidal concentrations and MICs of the NO donors against Pseudomonas aeruginosa were compared under aerobic and anaerobic conditions. Differential antibacterial activities were primarily the result of NO scavenging by oxygen under aerobic environments and not changes in bacterial physiology. Bacterial killing was also tested against nonmucoid and mucoid biofilms and compared to that of tobramycin. Smaller NO payloads were required to eradicate P. aeruginosa biofilms under anaerobic versus aerobic conditions. Under oxygen-free environments, the NO treatment was 10-fold more effective at killing biofilms than tobramycin. These results demonstrate the potential utility of NO-releasing chitosan oligosaccharides under both aerobic and anaerobic environments.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>26239983</pmid><doi>10.1128/AAC.01208-15</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Aerobiosis Anaerobiosis Anti-Bacterial Agents Anti-Bacterial Agents - chemical synthesis Anti-Bacterial Agents - pharmacology Azo Compounds - chemistry Biofilms Biofilms - drug effects Biofilms - growth & development Chitosan Chitosan - analogs & derivatives Chitosan - chemical synthesis Chitosan - pharmacology Glycosaminoglycans - biosynthesis Mechanisms of Action: Physiological Effects Microbial Sensitivity Tests Nitric Oxide Nitric Oxide - chemistry Nitric Oxide - pharmacology Nitric Oxide Donors Nitric Oxide Donors - chemical synthesis Nitric Oxide Donors - pharmacology Oxygen - pharmacology Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - growth & development Tobramycin - pharmacology |
| title | Antibacterial Action of Nitric Oxide-Releasing Chitosan Oligosaccharides against Pseudomonas aeruginosa under Aerobic and Anaerobic Conditions |
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