The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation
Expression of kisspeptin (protein) and Kiss1r (mRNA) was recently documented in the mouse uterus on D4 of pregnancy (the day of embryo implantation) suggesting that the uterine-based kisspeptin (KP)/kisspeptin receptor (KISS1R) signaling system regulates embryo implantation. Despite this important s...
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| Veröffentlicht in: | Reproductive biology and endocrinology 2015-09, Vol.13 (1), p.105-105, Article 105 |
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| creator | Fayazi, Mehri Calder, Michele Bhattacharya, Moshmi Vilos, George A Power, Stephen Babwah, Andy V |
| description | Expression of kisspeptin (protein) and Kiss1r (mRNA) was recently documented in the mouse uterus on D4 of pregnancy (the day of embryo implantation) suggesting that the uterine-based kisspeptin (KP)/kisspeptin receptor (KISS1R) signaling system regulates embryo implantation. Despite this important suggestion, it was never demonstrated that the uterus actually exhibits a functional KP/KISS1R signaling system on D4 of pregnancy. Thus, the goal of this study was to determine whether a functional KP/KISS1R signaling system exists in the mouse uterus on D4 of pregnancy.
Since kisspeptin/KISS1R signaling triggers the phosphorylation of the mitogen-activated protein kinases p38 and ERK1/2, through immunohistochemical analyses, we determined whether exogenously administered kisspeptin could trigger p38 and ERK1/2 phosphorylation in the uterus on D4 of pregnancy. The results clearly demonstrated that kisspeptin could and that its effects were mediated via KISS1R. Additionally, the robust kisspeptin-triggered response was observed in the pregnant uterus only. Finally, it was demonstrated that on D4 of pregnancy the Kiss1 null uterus expresses functional KISS1R molecules capable of mediating the effects of kisspeptin.
These results lead us to conclude that on D4 of pregnancy, the mouse uterus expresses a functional KP/KISS1R signaling system strengthening the possibility that this signaling system regulates embryo implantation. These findings strengthen the rationale for determining whether such a functional system exists in the uterus of the human female and if so, what role it might play in human pregnancy. |
| doi_str_mv | 10.1186/s12958-015-0105-1 |
| format | Article |
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Since kisspeptin/KISS1R signaling triggers the phosphorylation of the mitogen-activated protein kinases p38 and ERK1/2, through immunohistochemical analyses, we determined whether exogenously administered kisspeptin could trigger p38 and ERK1/2 phosphorylation in the uterus on D4 of pregnancy. The results clearly demonstrated that kisspeptin could and that its effects were mediated via KISS1R. Additionally, the robust kisspeptin-triggered response was observed in the pregnant uterus only. Finally, it was demonstrated that on D4 of pregnancy the Kiss1 null uterus expresses functional KISS1R molecules capable of mediating the effects of kisspeptin.
These results lead us to conclude that on D4 of pregnancy, the mouse uterus expresses a functional KP/KISS1R signaling system strengthening the possibility that this signaling system regulates embryo implantation. These findings strengthen the rationale for determining whether such a functional system exists in the uterus of the human female and if so, what role it might play in human pregnancy.</description><identifier>ISSN: 1477-7827</identifier><identifier>EISSN: 1477-7827</identifier><identifier>DOI: 10.1186/s12958-015-0105-1</identifier><identifier>PMID: 26384646</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Analysis ; Animals ; Embryo ; Embryo Implantation - physiology ; Embryonic development ; Embryos ; Experiments ; Female ; Females ; Genetic aspects ; Infertility ; Kinases ; Kisspeptins - physiology ; Mice ; Mice, Knockout ; Mitogens ; Phosphorylation ; Pregnancy ; Pregnant women ; Protein kinases ; Proteins ; Receptors, G-Protein-Coupled - physiology ; Receptors, Kisspeptin-1 ; RNA ; Rodents ; Short Communication ; Signal Transduction - physiology ; Studies ; Uterus - physiology</subject><ispartof>Reproductive biology and endocrinology, 2015-09, Vol.13 (1), p.105-105, Article 105</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2015</rights><rights>Fayazi et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-18870d9eea4adfc533969ffaff8f30d75ded53d1b9c601bfee924163cf0d54e83</citedby><cites>FETCH-LOGICAL-c494t-18870d9eea4adfc533969ffaff8f30d75ded53d1b9c601bfee924163cf0d54e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575475/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4575475/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26384646$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fayazi, Mehri</creatorcontrib><creatorcontrib>Calder, Michele</creatorcontrib><creatorcontrib>Bhattacharya, Moshmi</creatorcontrib><creatorcontrib>Vilos, George A</creatorcontrib><creatorcontrib>Power, Stephen</creatorcontrib><creatorcontrib>Babwah, Andy V</creatorcontrib><title>The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation</title><title>Reproductive biology and endocrinology</title><addtitle>Reprod Biol Endocrinol</addtitle><description>Expression of kisspeptin (protein) and Kiss1r (mRNA) was recently documented in the mouse uterus on D4 of pregnancy (the day of embryo implantation) suggesting that the uterine-based kisspeptin (KP)/kisspeptin receptor (KISS1R) signaling system regulates embryo implantation. Despite this important suggestion, it was never demonstrated that the uterus actually exhibits a functional KP/KISS1R signaling system on D4 of pregnancy. Thus, the goal of this study was to determine whether a functional KP/KISS1R signaling system exists in the mouse uterus on D4 of pregnancy.
Since kisspeptin/KISS1R signaling triggers the phosphorylation of the mitogen-activated protein kinases p38 and ERK1/2, through immunohistochemical analyses, we determined whether exogenously administered kisspeptin could trigger p38 and ERK1/2 phosphorylation in the uterus on D4 of pregnancy. The results clearly demonstrated that kisspeptin could and that its effects were mediated via KISS1R. Additionally, the robust kisspeptin-triggered response was observed in the pregnant uterus only. Finally, it was demonstrated that on D4 of pregnancy the Kiss1 null uterus expresses functional KISS1R molecules capable of mediating the effects of kisspeptin.
These results lead us to conclude that on D4 of pregnancy, the mouse uterus expresses a functional KP/KISS1R signaling system strengthening the possibility that this signaling system regulates embryo implantation. These findings strengthen the rationale for determining whether such a functional system exists in the uterus of the human female and if so, what role it might play in human pregnancy.</description><subject>Analysis</subject><subject>Animals</subject><subject>Embryo</subject><subject>Embryo Implantation - physiology</subject><subject>Embryonic development</subject><subject>Embryos</subject><subject>Experiments</subject><subject>Female</subject><subject>Females</subject><subject>Genetic aspects</subject><subject>Infertility</subject><subject>Kinases</subject><subject>Kisspeptins - physiology</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Mitogens</subject><subject>Phosphorylation</subject><subject>Pregnancy</subject><subject>Pregnant women</subject><subject>Protein kinases</subject><subject>Proteins</subject><subject>Receptors, G-Protein-Coupled - physiology</subject><subject>Receptors, Kisspeptin-1</subject><subject>RNA</subject><subject>Rodents</subject><subject>Short Communication</subject><subject>Signal Transduction - physiology</subject><subject>Studies</subject><subject>Uterus - physiology</subject><issn>1477-7827</issn><issn>1477-7827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNptUl1rFTEUXESxtfoDfJGAL75sm-zmY_dFKEVrsSDY-hyyycm9qbvJmmSL9983y621FQkhh2RmTuYwVfWW4GNCOn6SSNOzrsaElY1ZTZ5Vh4QKUYuuEc8f1QfVq5RuMG4w7vjL6qDhbUc55YdVvt4CmiNsvPIZTWFJgJYMcUkIfm_d4HJCCtnF6-yCVyP66VKaYc7On3y9uLoi31FyhTw6v0FplzJMKHiUi6pROxQsgmmIu4DcNI-lhVplXlcvrBoTvLk_j6ofnz9dn32pL7-dX5ydXtaa9jTXpOsENj2AospYzdq25721ytrOttgIZsCw1pCh1xyTwQL0DSW81RYbRqFrj6qPe915GSYwGnyOapRzdJOKOxmUk09fvNvKTbiVlAlGBSsCH-4FYvi1QMpycknDWJxAGZUkgvC-FZyRAn3_D_QmLLEMZkV1uGGiadhf1EaNIJ23ofTVq6g8ZZQwzIvHgjr-D6osA5PTwYN15f4JgewJOoaUItgHjwTLNSpyHxVZoiLXqMj1w-8eD-eB8Scb7R3f0btX</recordid><startdate>20150918</startdate><enddate>20150918</enddate><creator>Fayazi, Mehri</creator><creator>Calder, Michele</creator><creator>Bhattacharya, Moshmi</creator><creator>Vilos, George A</creator><creator>Power, Stephen</creator><creator>Babwah, Andy V</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150918</creationdate><title>The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation</title><author>Fayazi, Mehri ; Calder, Michele ; Bhattacharya, Moshmi ; Vilos, George A ; Power, Stephen ; Babwah, Andy V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-18870d9eea4adfc533969ffaff8f30d75ded53d1b9c601bfee924163cf0d54e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Embryo</topic><topic>Embryo Implantation - physiology</topic><topic>Embryonic development</topic><topic>Embryos</topic><topic>Experiments</topic><topic>Female</topic><topic>Females</topic><topic>Genetic aspects</topic><topic>Infertility</topic><topic>Kinases</topic><topic>Kisspeptins - physiology</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Mitogens</topic><topic>Phosphorylation</topic><topic>Pregnancy</topic><topic>Pregnant women</topic><topic>Protein kinases</topic><topic>Proteins</topic><topic>Receptors, G-Protein-Coupled - physiology</topic><topic>Receptors, Kisspeptin-1</topic><topic>RNA</topic><topic>Rodents</topic><topic>Short Communication</topic><topic>Signal Transduction - physiology</topic><topic>Studies</topic><topic>Uterus - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fayazi, Mehri</creatorcontrib><creatorcontrib>Calder, Michele</creatorcontrib><creatorcontrib>Bhattacharya, Moshmi</creatorcontrib><creatorcontrib>Vilos, George A</creatorcontrib><creatorcontrib>Power, Stephen</creatorcontrib><creatorcontrib>Babwah, Andy V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Reproductive biology and endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fayazi, Mehri</au><au>Calder, Michele</au><au>Bhattacharya, Moshmi</au><au>Vilos, George A</au><au>Power, Stephen</au><au>Babwah, Andy V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation</atitle><jtitle>Reproductive biology and endocrinology</jtitle><addtitle>Reprod Biol Endocrinol</addtitle><date>2015-09-18</date><risdate>2015</risdate><volume>13</volume><issue>1</issue><spage>105</spage><epage>105</epage><pages>105-105</pages><artnum>105</artnum><issn>1477-7827</issn><eissn>1477-7827</eissn><abstract>Expression of kisspeptin (protein) and Kiss1r (mRNA) was recently documented in the mouse uterus on D4 of pregnancy (the day of embryo implantation) suggesting that the uterine-based kisspeptin (KP)/kisspeptin receptor (KISS1R) signaling system regulates embryo implantation. Despite this important suggestion, it was never demonstrated that the uterus actually exhibits a functional KP/KISS1R signaling system on D4 of pregnancy. Thus, the goal of this study was to determine whether a functional KP/KISS1R signaling system exists in the mouse uterus on D4 of pregnancy.
Since kisspeptin/KISS1R signaling triggers the phosphorylation of the mitogen-activated protein kinases p38 and ERK1/2, through immunohistochemical analyses, we determined whether exogenously administered kisspeptin could trigger p38 and ERK1/2 phosphorylation in the uterus on D4 of pregnancy. The results clearly demonstrated that kisspeptin could and that its effects were mediated via KISS1R. Additionally, the robust kisspeptin-triggered response was observed in the pregnant uterus only. Finally, it was demonstrated that on D4 of pregnancy the Kiss1 null uterus expresses functional KISS1R molecules capable of mediating the effects of kisspeptin.
These results lead us to conclude that on D4 of pregnancy, the mouse uterus expresses a functional KP/KISS1R signaling system strengthening the possibility that this signaling system regulates embryo implantation. These findings strengthen the rationale for determining whether such a functional system exists in the uterus of the human female and if so, what role it might play in human pregnancy.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26384646</pmid><doi>10.1186/s12958-015-0105-1</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; SpringerNature Journals; PubMed Central Open Access; Springer Nature OA Free Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Analysis Animals Embryo Embryo Implantation - physiology Embryonic development Embryos Experiments Female Females Genetic aspects Infertility Kinases Kisspeptins - physiology Mice Mice, Knockout Mitogens Phosphorylation Pregnancy Pregnant women Protein kinases Proteins Receptors, G-Protein-Coupled - physiology Receptors, Kisspeptin-1 RNA Rodents Short Communication Signal Transduction - physiology Studies Uterus - physiology |
| title | The pregnant mouse uterus exhibits a functional kisspeptin/KISS1R signaling system on the day of embryo implantation |
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