Proteomic analysis of blood level of proteins before and after operation in patients with esophageal squamous cell carcinoma at high-incidence area in Henan Province
To characterize the protein files in blood from same patients with esophageal squamous cell carcinoma (ESCC) before and after operation at the high-incidence area for ESCC in Henan Province, China. Two-dimensional electrophoresis, silver staining and ImageMaster 2-DE analysis software were applied t...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2004-11, Vol.10 (22), p.3365-3368 |
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| container_title | World journal of gastroenterology : WJG |
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| creator | An, Ji-Ye Fan, Zong-Min Zhuang, Ze-Hao Qin, Yan-Ru Gao, Shan-Shan Li, Ji-Lin Wang, Li-Dong |
| description | To characterize the protein files in blood from same patients with esophageal squamous cell carcinoma (ESCC) before and after operation at the high-incidence area for ESCC in Henan Province, China.
Two-dimensional electrophoresis, silver staining and ImageMaster 2-DE analysis software were applied to the determination of protein files in the blood obtained from normal controls and ESCC patients before and after operation.
A total of 655, 662 and 677 protein spots were identified, respectively, from the normal controls and ESCC patients before and after operation. No significant difference in the number of protein spots was observed between the normal group and ESCC patients. A total of seven protein spots were identified with a dramatic difference among the samples before and after operation. Six protein spots were up-regulated and one protein spot was down-regulated in the group after operation compared with those in normal and before operation. Three protein spots were further characterized by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS). The proteins from these three spots were identified as serum amyloid A (SAA), amyloid related serum protein and haptoglobin.
Serum amyloid A, amyloid related serum protein and haptoglobin may be related with ESCC and/or surgery. The significance of these proteins needs to be further characterized. The present study provides informative data for the establishment of serum protein profiles related with ESCC. |
| doi_str_mv | 10.3748/wjg.v10.i22.3365 |
| format | Article |
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Two-dimensional electrophoresis, silver staining and ImageMaster 2-DE analysis software were applied to the determination of protein files in the blood obtained from normal controls and ESCC patients before and after operation.
A total of 655, 662 and 677 protein spots were identified, respectively, from the normal controls and ESCC patients before and after operation. No significant difference in the number of protein spots was observed between the normal group and ESCC patients. A total of seven protein spots were identified with a dramatic difference among the samples before and after operation. Six protein spots were up-regulated and one protein spot was down-regulated in the group after operation compared with those in normal and before operation. Three protein spots were further characterized by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS). The proteins from these three spots were identified as serum amyloid A (SAA), amyloid related serum protein and haptoglobin.
Serum amyloid A, amyloid related serum protein and haptoglobin may be related with ESCC and/or surgery. The significance of these proteins needs to be further characterized. The present study provides informative data for the establishment of serum protein profiles related with ESCC.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v10.i22.3365</identifier><identifier>PMID: 15484320</identifier><language>eng</language><publisher>United States: Laboratory for Cancer Research and the Third Teaching Hospital, College of Medicine, Zhengzhou University, Zhengzhou 450052, Henan Province, China%Laboratory for Cancer Research, College of Medicine,Zhengzhou University, Zhengzhou 450052, Henan Province, China%Department of Pathology, Yaocun Esophageal Cancer Hospital, Linzhou 456592, Henan Province, China</publisher><subject>Aged ; Blood Proteins - analysis ; Brief Reports ; Carcinoma, Squamous Cell - blood ; Carcinoma, Squamous Cell - epidemiology ; Carcinoma, Squamous Cell - surgery ; China - epidemiology ; Electrophoresis, Gel, Two-Dimensional ; Esophageal Neoplasms - blood ; Esophageal Neoplasms - epidemiology ; Esophageal Neoplasms - surgery ; Female ; Humans ; Incidence ; Male ; Middle Aged ; Proteomics ; Silver Staining ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><ispartof>World journal of gastroenterology : WJG, 2004-11, Vol.10 (22), p.3365-3368</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved. 2004</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-34940995694e1b6691be252332af1f039ce211b5270b099de56c70ae1540daa53</citedby><cites>FETCH-LOGICAL-c421t-34940995694e1b6691be252332af1f039ce211b5270b099de56c70ae1540daa53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/wjg/wjg.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4572315/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4572315/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15484320$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>An, Ji-Ye</creatorcontrib><creatorcontrib>Fan, Zong-Min</creatorcontrib><creatorcontrib>Zhuang, Ze-Hao</creatorcontrib><creatorcontrib>Qin, Yan-Ru</creatorcontrib><creatorcontrib>Gao, Shan-Shan</creatorcontrib><creatorcontrib>Li, Ji-Lin</creatorcontrib><creatorcontrib>Wang, Li-Dong</creatorcontrib><title>Proteomic analysis of blood level of proteins before and after operation in patients with esophageal squamous cell carcinoma at high-incidence area in Henan Province</title><title>World journal of gastroenterology : WJG</title><addtitle>World J Gastroenterol</addtitle><description>To characterize the protein files in blood from same patients with esophageal squamous cell carcinoma (ESCC) before and after operation at the high-incidence area for ESCC in Henan Province, China.
Two-dimensional electrophoresis, silver staining and ImageMaster 2-DE analysis software were applied to the determination of protein files in the blood obtained from normal controls and ESCC patients before and after operation.
A total of 655, 662 and 677 protein spots were identified, respectively, from the normal controls and ESCC patients before and after operation. No significant difference in the number of protein spots was observed between the normal group and ESCC patients. A total of seven protein spots were identified with a dramatic difference among the samples before and after operation. Six protein spots were up-regulated and one protein spot was down-regulated in the group after operation compared with those in normal and before operation. Three protein spots were further characterized by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS). The proteins from these three spots were identified as serum amyloid A (SAA), amyloid related serum protein and haptoglobin.
Serum amyloid A, amyloid related serum protein and haptoglobin may be related with ESCC and/or surgery. The significance of these proteins needs to be further characterized. The present study provides informative data for the establishment of serum protein profiles related with ESCC.</description><subject>Aged</subject><subject>Blood Proteins - analysis</subject><subject>Brief Reports</subject><subject>Carcinoma, Squamous Cell - blood</subject><subject>Carcinoma, Squamous Cell - epidemiology</subject><subject>Carcinoma, Squamous Cell - surgery</subject><subject>China - epidemiology</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Esophageal Neoplasms - blood</subject><subject>Esophageal Neoplasms - epidemiology</subject><subject>Esophageal Neoplasms - surgery</subject><subject>Female</subject><subject>Humans</subject><subject>Incidence</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Proteomics</subject><subject>Silver Staining</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUk1v1DAQtRAVXQp3TsgHxC2Lv5JsLkioKhSpEj20Z2viTBKvEju1k131B_E_cdgVHyd7NG_ezLx5hLzjbCtLtft03HfbQwqsEFspi_wF2QjBq0zsFHtJNpyxMqukKC_J6xj3jAkpc_GKXPJc7ZQUbEN-3gc_ox-toeBgeI42Ut_SevC-oQMecFjDaQVZF2mNrQ-YoA2FdsZA_YQBZusdtY5O6YdujvRo555i9FMPHcJA49MCo18iNTgM1EAw1vkRKMy0t12fWWdsg84k5oCwUt2iA0fTcIeUwzfkooUh4tvze0Uev948XN9mdz--fb_-cpcZJficSVUpVlV5USnkdVFUvEaRp6UFtLxlsjIoOK9zUbI64RrMC1MywCQHawByeUU-n3inpR6xMWmZAIOegh0hPGsPVv-fcbbXnT9olZdC8pXgw4ngCK4F1-m9X0LSNep0KsGYEoJJlmAfz32Cf1owznq0cdUGHCaZdBq9LMRvPnYCmuBjDNj-mYUzvVpg5dXJAjpZQK8WSCXv_93hb8H55vIX3maxaQ</recordid><startdate>20041115</startdate><enddate>20041115</enddate><creator>An, Ji-Ye</creator><creator>Fan, Zong-Min</creator><creator>Zhuang, Ze-Hao</creator><creator>Qin, Yan-Ru</creator><creator>Gao, Shan-Shan</creator><creator>Li, Ji-Lin</creator><creator>Wang, Li-Dong</creator><general>Laboratory for Cancer Research and the Third Teaching Hospital, College of Medicine, Zhengzhou University, Zhengzhou 450052, Henan Province, China%Laboratory for Cancer Research, College of Medicine,Zhengzhou University, Zhengzhou 450052, Henan Province, China%Department of Pathology, Yaocun Esophageal Cancer Hospital, Linzhou 456592, Henan Province, China</general><general>Baishideng Publishing Group Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20041115</creationdate><title>Proteomic analysis of blood level of proteins before and after operation in patients with esophageal squamous cell carcinoma at high-incidence area in Henan Province</title><author>An, Ji-Ye ; Fan, Zong-Min ; Zhuang, Ze-Hao ; Qin, Yan-Ru ; Gao, Shan-Shan ; Li, Ji-Lin ; Wang, Li-Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-34940995694e1b6691be252332af1f039ce211b5270b099de56c70ae1540daa53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Aged</topic><topic>Blood Proteins - analysis</topic><topic>Brief Reports</topic><topic>Carcinoma, Squamous Cell - blood</topic><topic>Carcinoma, Squamous Cell - epidemiology</topic><topic>Carcinoma, Squamous Cell - surgery</topic><topic>China - epidemiology</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Esophageal Neoplasms - blood</topic><topic>Esophageal Neoplasms - epidemiology</topic><topic>Esophageal Neoplasms - surgery</topic><topic>Female</topic><topic>Humans</topic><topic>Incidence</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Proteomics</topic><topic>Silver Staining</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><toplevel>online_resources</toplevel><creatorcontrib>An, Ji-Ye</creatorcontrib><creatorcontrib>Fan, Zong-Min</creatorcontrib><creatorcontrib>Zhuang, Ze-Hao</creatorcontrib><creatorcontrib>Qin, Yan-Ru</creatorcontrib><creatorcontrib>Gao, Shan-Shan</creatorcontrib><creatorcontrib>Li, Ji-Lin</creatorcontrib><creatorcontrib>Wang, Li-Dong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>An, Ji-Ye</au><au>Fan, Zong-Min</au><au>Zhuang, Ze-Hao</au><au>Qin, Yan-Ru</au><au>Gao, Shan-Shan</au><au>Li, Ji-Lin</au><au>Wang, Li-Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic analysis of blood level of proteins before and after operation in patients with esophageal squamous cell carcinoma at high-incidence area in Henan Province</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World J Gastroenterol</addtitle><date>2004-11-15</date><risdate>2004</risdate><volume>10</volume><issue>22</issue><spage>3365</spage><epage>3368</epage><pages>3365-3368</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>To characterize the protein files in blood from same patients with esophageal squamous cell carcinoma (ESCC) before and after operation at the high-incidence area for ESCC in Henan Province, China.
Two-dimensional electrophoresis, silver staining and ImageMaster 2-DE analysis software were applied to the determination of protein files in the blood obtained from normal controls and ESCC patients before and after operation.
A total of 655, 662 and 677 protein spots were identified, respectively, from the normal controls and ESCC patients before and after operation. No significant difference in the number of protein spots was observed between the normal group and ESCC patients. A total of seven protein spots were identified with a dramatic difference among the samples before and after operation. Six protein spots were up-regulated and one protein spot was down-regulated in the group after operation compared with those in normal and before operation. Three protein spots were further characterized by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS). The proteins from these three spots were identified as serum amyloid A (SAA), amyloid related serum protein and haptoglobin.
Serum amyloid A, amyloid related serum protein and haptoglobin may be related with ESCC and/or surgery. The significance of these proteins needs to be further characterized. The present study provides informative data for the establishment of serum protein profiles related with ESCC.</abstract><cop>United States</cop><pub>Laboratory for Cancer Research and the Third Teaching Hospital, College of Medicine, Zhengzhou University, Zhengzhou 450052, Henan Province, China%Laboratory for Cancer Research, College of Medicine,Zhengzhou University, Zhengzhou 450052, Henan Province, China%Department of Pathology, Yaocun Esophageal Cancer Hospital, Linzhou 456592, Henan Province, China</pub><pmid>15484320</pmid><doi>10.3748/wjg.v10.i22.3365</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1007-9327 |
| ispartof | World journal of gastroenterology : WJG, 2004-11, Vol.10 (22), p.3365-3368 |
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| subjects | Aged Blood Proteins - analysis Brief Reports Carcinoma, Squamous Cell - blood Carcinoma, Squamous Cell - epidemiology Carcinoma, Squamous Cell - surgery China - epidemiology Electrophoresis, Gel, Two-Dimensional Esophageal Neoplasms - blood Esophageal Neoplasms - epidemiology Esophageal Neoplasms - surgery Female Humans Incidence Male Middle Aged Proteomics Silver Staining Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title | Proteomic analysis of blood level of proteins before and after operation in patients with esophageal squamous cell carcinoma at high-incidence area in Henan Province |
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