Effects of tegaserod on Fos, substance P and calcitonin gene-related peptide expression induced by colon inflammation in lumbarsacral spinal cord
To investigate the mechanisms of tegaserod, a partial 5-HT4 agonist, in reducing visceral sensitivity by observing Fos, substance P (SP) and calcitonin gene-related peptide (CGRP) expression in the lumbarsacral spinal cord induced by colonic inflammation in rats. Twenty-four male rats with colonic i...
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| Veröffentlicht in: | World journal of gastroenterology : WJG 2004-06, Vol.10 (12), p.1830-1833 |
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| description | To investigate the mechanisms of tegaserod, a partial 5-HT4 agonist, in reducing visceral sensitivity by observing Fos, substance P (SP) and calcitonin gene-related peptide (CGRP) expression in the lumbarsacral spinal cord induced by colonic inflammation in rats.
Twenty-four male rats with colonic inflammation induced by intraluminal instillation of trinitrobenzenesulfonic acid (TNBS) were divided into 3 groups. Treatment group 1: intra-gastric administration of tegaserod, 2 mg/kg.d; Treatment group 2: intra-gastric administration of tegaserod, 1 mg/kg.d;
intra-gastric administration of saline, 2.0 mL/d. After 7 d of intra-gastric administration, lumbarsacral spinal cord was removed and processed for Fos, SP and CGRP immunohistochemistry.
In rats of the control group, the majority of Fos labeled neurons was localized in deeper laminae of the lumbarsacral spinal cord (L(5)-S(1)). SP and CGRP were primarily expressed in the superficial laminae of the spinal cord after TNBS injection. Intra-gastric administration of tegaserod (2 mg/kg.d) resulted in a significant decrease of Fos labeled neurons (22.0+/-7.7) and SP density (12.5+/-1.4) in the dorsal horn in the lumbarsacral spinal cord compared to those of the control group (62.2+/-18.9, 35.9+/-8.9, P0.05). Neither Fos expression nor SP or CGRP density in the dorsal horn significantly declined in rats of treatment group 2 compared to those of the control group (P>0.05).
Tegaserod can significantly reduce Fos labeled neurons in the lumbarsacral spinal cord induced by colonic inflammation. Tegaserod may reduce visceral sensitivity by inhibiting SP expression in the dorsal horn of spinal cord. |
| doi_str_mv | 10.3748/wjg.v10.i12.1830 |
| format | Article |
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Twenty-four male rats with colonic inflammation induced by intraluminal instillation of trinitrobenzenesulfonic acid (TNBS) were divided into 3 groups. Treatment group 1: intra-gastric administration of tegaserod, 2 mg/kg.d; Treatment group 2: intra-gastric administration of tegaserod, 1 mg/kg.d;
intra-gastric administration of saline, 2.0 mL/d. After 7 d of intra-gastric administration, lumbarsacral spinal cord was removed and processed for Fos, SP and CGRP immunohistochemistry.
In rats of the control group, the majority of Fos labeled neurons was localized in deeper laminae of the lumbarsacral spinal cord (L(5)-S(1)). SP and CGRP were primarily expressed in the superficial laminae of the spinal cord after TNBS injection. Intra-gastric administration of tegaserod (2 mg/kg.d) resulted in a significant decrease of Fos labeled neurons (22.0+/-7.7) and SP density (12.5+/-1.4) in the dorsal horn in the lumbarsacral spinal cord compared to those of the control group (62.2+/-18.9, 35.9+/-8.9, P<0.05). However, CGRP content in dorsal horn did not significantly reduce in rats of treatment group 1 (1.2+/-1.1) compared to that of the control group (2.8+/-2.4, P>0.05). Neither Fos expression nor SP or CGRP density in the dorsal horn significantly declined in rats of treatment group 2 compared to those of the control group (P>0.05).
Tegaserod can significantly reduce Fos labeled neurons in the lumbarsacral spinal cord induced by colonic inflammation. Tegaserod may reduce visceral sensitivity by inhibiting SP expression in the dorsal horn of spinal cord.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><identifier>DOI: 10.3748/wjg.v10.i12.1830</identifier><identifier>PMID: 15188517</identifier><language>eng</language><publisher>United States: Department of Gastroenterology,Second Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China</publisher><subject>Animals ; Brief Reports ; Calcitonin Gene-Related Peptide - metabolism ; Colitis - complications ; Colitis - drug therapy ; Colitis - metabolism ; Disease Models, Animal ; Gastrointestinal Agents - pharmacology ; Indoles - pharmacology ; Male ; Pain - drug therapy ; Pain - etiology ; Pain - metabolism ; Proto-Oncogene Proteins c-fos - metabolism ; Rats ; Rats, Sprague-Dawley ; Spinal Cord - drug effects ; Spinal Cord - metabolism ; Substance P - metabolism ; Visceral Afferents - drug effects ; Visceral Afferents - metabolism</subject><ispartof>World journal of gastroenterology : WJG, 2004-06, Vol.10 (12), p.1830-1833</ispartof><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>The Author(s) 2004. Published by Baishideng Publishing Group Inc. All rights reserved. 2004</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-317c1d2b8c3fde247db87f9dfc301538b278e956cafb1732c34d3f4c7941bcb33</citedby><cites>FETCH-LOGICAL-c487t-317c1d2b8c3fde247db87f9dfc301538b278e956cafb1732c34d3f4c7941bcb33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/wjg/wjg.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4572280/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4572280/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15188517$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Yi-Ning</creatorcontrib><creatorcontrib>Luo, Jin-Yan</creatorcontrib><title>Effects of tegaserod on Fos, substance P and calcitonin gene-related peptide expression induced by colon inflammation in lumbarsacral spinal cord</title><title>World journal of gastroenterology : WJG</title><addtitle>World J Gastroenterol</addtitle><description>To investigate the mechanisms of tegaserod, a partial 5-HT4 agonist, in reducing visceral sensitivity by observing Fos, substance P (SP) and calcitonin gene-related peptide (CGRP) expression in the lumbarsacral spinal cord induced by colonic inflammation in rats.
Twenty-four male rats with colonic inflammation induced by intraluminal instillation of trinitrobenzenesulfonic acid (TNBS) were divided into 3 groups. Treatment group 1: intra-gastric administration of tegaserod, 2 mg/kg.d; Treatment group 2: intra-gastric administration of tegaserod, 1 mg/kg.d;
intra-gastric administration of saline, 2.0 mL/d. After 7 d of intra-gastric administration, lumbarsacral spinal cord was removed and processed for Fos, SP and CGRP immunohistochemistry.
In rats of the control group, the majority of Fos labeled neurons was localized in deeper laminae of the lumbarsacral spinal cord (L(5)-S(1)). SP and CGRP were primarily expressed in the superficial laminae of the spinal cord after TNBS injection. Intra-gastric administration of tegaserod (2 mg/kg.d) resulted in a significant decrease of Fos labeled neurons (22.0+/-7.7) and SP density (12.5+/-1.4) in the dorsal horn in the lumbarsacral spinal cord compared to those of the control group (62.2+/-18.9, 35.9+/-8.9, P<0.05). However, CGRP content in dorsal horn did not significantly reduce in rats of treatment group 1 (1.2+/-1.1) compared to that of the control group (2.8+/-2.4, P>0.05). Neither Fos expression nor SP or CGRP density in the dorsal horn significantly declined in rats of treatment group 2 compared to those of the control group (P>0.05).
Tegaserod can significantly reduce Fos labeled neurons in the lumbarsacral spinal cord induced by colonic inflammation. Tegaserod may reduce visceral sensitivity by inhibiting SP expression in the dorsal horn of spinal cord.</description><subject>Animals</subject><subject>Brief Reports</subject><subject>Calcitonin Gene-Related Peptide - metabolism</subject><subject>Colitis - complications</subject><subject>Colitis - drug therapy</subject><subject>Colitis - metabolism</subject><subject>Disease Models, Animal</subject><subject>Gastrointestinal Agents - pharmacology</subject><subject>Indoles - pharmacology</subject><subject>Male</subject><subject>Pain - drug therapy</subject><subject>Pain - etiology</subject><subject>Pain - metabolism</subject><subject>Proto-Oncogene Proteins c-fos - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spinal Cord - drug effects</subject><subject>Spinal Cord - metabolism</subject><subject>Substance P - metabolism</subject><subject>Visceral Afferents - drug effects</subject><subject>Visceral Afferents - metabolism</subject><issn>1007-9327</issn><issn>2219-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkctuFDEQRS0EIkNgzwp5gVjRgx_d2LNBiqIEkCLBAtaWH-XGo267sd0J-Qz-GA8z4rEq2ffWrSodhJ5TsuWil2_u9uP2tj0CZVsqOXmANozRXcdkTx6iDSVEdDvOxBl6UsqeEMb5wB6jMzpQKQcqNujnlfdga8HJ4wqjLpCTwyni61Re47KaUnW0gD9jHR22erKhphgiHiFCl2HSFRxeYKnBAYYfS4ZSQusP0a22SeYe2zT9_vCTnmddjyqe1tnoXLTNesJlCbEVm7J7ih55PRV4dqrn6Ov11ZfLD93Np_cfLy9uOttLUTtOhaWOGWm5d8B64YwUfue85YQOXBomJOyGt1Z7QwVnlveO-96KXU-NNZyfo3fH3GU1MzgLsbZN1JLDrPO9Sjqo_5UYvqkx3ap-EIxJ0gJeHgPudPQ6jmqf1tyuKKphYYT0lBF-sL06zcnp-wqlqjkUC9OkI6S1KNGsrB9oM5Kj0eZUSgb_ZxdK1AH3IVc13KrhVgfcreXFvzf8bTjx5b8Af8WrNg</recordid><startdate>20040615</startdate><enddate>20040615</enddate><creator>Sun, Yi-Ning</creator><creator>Luo, Jin-Yan</creator><general>Department of Gastroenterology,Second Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China</general><general>Baishideng Publishing Group Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>20040615</creationdate><title>Effects of tegaserod on Fos, substance P and calcitonin gene-related peptide expression induced by colon inflammation in lumbarsacral spinal cord</title><author>Sun, Yi-Ning ; Luo, Jin-Yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-317c1d2b8c3fde247db87f9dfc301538b278e956cafb1732c34d3f4c7941bcb33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Brief Reports</topic><topic>Calcitonin Gene-Related Peptide - metabolism</topic><topic>Colitis - complications</topic><topic>Colitis - drug therapy</topic><topic>Colitis - metabolism</topic><topic>Disease Models, Animal</topic><topic>Gastrointestinal Agents - pharmacology</topic><topic>Indoles - pharmacology</topic><topic>Male</topic><topic>Pain - drug therapy</topic><topic>Pain - etiology</topic><topic>Pain - metabolism</topic><topic>Proto-Oncogene Proteins c-fos - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Spinal Cord - drug effects</topic><topic>Spinal Cord - metabolism</topic><topic>Substance P - metabolism</topic><topic>Visceral Afferents - drug effects</topic><topic>Visceral Afferents - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Sun, Yi-Ning</creatorcontrib><creatorcontrib>Luo, Jin-Yan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Wanfang Data Journals - Hong Kong</collection><collection>WANFANG Data Centre</collection><collection>Wanfang Data Journals</collection><collection>万方数据期刊 - 香港版</collection><collection>China Online Journals (COJ)</collection><collection>China Online Journals (COJ)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>World journal of gastroenterology : WJG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Yi-Ning</au><au>Luo, Jin-Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of tegaserod on Fos, substance P and calcitonin gene-related peptide expression induced by colon inflammation in lumbarsacral spinal cord</atitle><jtitle>World journal of gastroenterology : WJG</jtitle><addtitle>World J Gastroenterol</addtitle><date>2004-06-15</date><risdate>2004</risdate><volume>10</volume><issue>12</issue><spage>1830</spage><epage>1833</epage><pages>1830-1833</pages><issn>1007-9327</issn><eissn>2219-2840</eissn><abstract>To investigate the mechanisms of tegaserod, a partial 5-HT4 agonist, in reducing visceral sensitivity by observing Fos, substance P (SP) and calcitonin gene-related peptide (CGRP) expression in the lumbarsacral spinal cord induced by colonic inflammation in rats.
Twenty-four male rats with colonic inflammation induced by intraluminal instillation of trinitrobenzenesulfonic acid (TNBS) were divided into 3 groups. Treatment group 1: intra-gastric administration of tegaserod, 2 mg/kg.d; Treatment group 2: intra-gastric administration of tegaserod, 1 mg/kg.d;
intra-gastric administration of saline, 2.0 mL/d. After 7 d of intra-gastric administration, lumbarsacral spinal cord was removed and processed for Fos, SP and CGRP immunohistochemistry.
In rats of the control group, the majority of Fos labeled neurons was localized in deeper laminae of the lumbarsacral spinal cord (L(5)-S(1)). SP and CGRP were primarily expressed in the superficial laminae of the spinal cord after TNBS injection. Intra-gastric administration of tegaserod (2 mg/kg.d) resulted in a significant decrease of Fos labeled neurons (22.0+/-7.7) and SP density (12.5+/-1.4) in the dorsal horn in the lumbarsacral spinal cord compared to those of the control group (62.2+/-18.9, 35.9+/-8.9, P<0.05). However, CGRP content in dorsal horn did not significantly reduce in rats of treatment group 1 (1.2+/-1.1) compared to that of the control group (2.8+/-2.4, P>0.05). Neither Fos expression nor SP or CGRP density in the dorsal horn significantly declined in rats of treatment group 2 compared to those of the control group (P>0.05).
Tegaserod can significantly reduce Fos labeled neurons in the lumbarsacral spinal cord induced by colonic inflammation. Tegaserod may reduce visceral sensitivity by inhibiting SP expression in the dorsal horn of spinal cord.</abstract><cop>United States</cop><pub>Department of Gastroenterology,Second Hospital of Xi'an Jiaotong University, Xi'an 710004, Shaanxi Province, China</pub><pmid>15188517</pmid><doi>10.3748/wjg.v10.i12.1830</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Brief Reports Calcitonin Gene-Related Peptide - metabolism Colitis - complications Colitis - drug therapy Colitis - metabolism Disease Models, Animal Gastrointestinal Agents - pharmacology Indoles - pharmacology Male Pain - drug therapy Pain - etiology Pain - metabolism Proto-Oncogene Proteins c-fos - metabolism Rats Rats, Sprague-Dawley Spinal Cord - drug effects Spinal Cord - metabolism Substance P - metabolism Visceral Afferents - drug effects Visceral Afferents - metabolism |
| title | Effects of tegaserod on Fos, substance P and calcitonin gene-related peptide expression induced by colon inflammation in lumbarsacral spinal cord |
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