Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serum
Bone marrow-derived mesenchymal stromal cells (MSCs) have been intensely studied for the purpose of developing solutions for clinical tissue engineering. Autologous MSCs can potentially be used to replace tissue defects, but the procedure also carries risks such as immunization and xenogeneic infect...
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| Veröffentlicht in: | Stem cell research & therapy 2015-09, Vol.6 (1), p.167-167, Article 167 |
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| creator | Castrén, Eeva Sillat, Tarvo Oja, Sofia Noro, Ariel Laitinen, Anita Konttinen, Yrjö T Lehenkari, Petri Hukkanen, Mika Korhonen, Matti |
| description | Bone marrow-derived mesenchymal stromal cells (MSCs) have been intensely studied for the purpose of developing solutions for clinical tissue engineering. Autologous MSCs can potentially be used to replace tissue defects, but the procedure also carries risks such as immunization and xenogeneic infection. Replacement of the commonly used fetal calf serum (FCS) with human platelet lysate and plasma (PLP) to support cell growth may reduce some of these risks. Altered media could, however, influence stem cell differentiation and we address this experimentally.
We examined human MSC differentiation into the osteoblast lineage using in vitro two- and three-dimensional cultures with PLP or FCS as cell culture medium supplements. Differentiation was followed by quantitative polymerase chain reaction, and alkaline phosphatase activity, matrix formation and matrix calcium content were quantified.
Three-dimensional culture, where human MSCs were grown on collagen sponges, markedly stimulated osteoblast differentiation; a fourfold increase in calcium deposition could be observed in both PLP and FCS groups. PLP-grown cells showed robust osteogenic differentiation both in two- and three-dimensional MSC cultures. The calcium content of the matrix in the two-dimensional PLP group at day 14 was 2.2-fold higher in comparison to the FCS group (p |
| doi_str_mv | 10.1186/s13287-015-0162-6 |
| format | Article |
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We examined human MSC differentiation into the osteoblast lineage using in vitro two- and three-dimensional cultures with PLP or FCS as cell culture medium supplements. Differentiation was followed by quantitative polymerase chain reaction, and alkaline phosphatase activity, matrix formation and matrix calcium content were quantified.
Three-dimensional culture, where human MSCs were grown on collagen sponges, markedly stimulated osteoblast differentiation; a fourfold increase in calcium deposition could be observed in both PLP and FCS groups. PLP-grown cells showed robust osteogenic differentiation both in two- and three-dimensional MSC cultures. The calcium content of the matrix in the two-dimensional PLP group at day 14 was 2.2-fold higher in comparison to the FCS group (p < 0.0001), and at day 21 it was still 1.3-fold higher (p < 0.001), suggesting earlier calcium accumulation to the matrix in the PLP group. This was supported by stronger Alizarin Red staining in the PLP group at day 14. In two-dimesional PLP cultures, cellular proliferation appeared to decrease during later stages of differentiation, while in the FCS group the number of cells increased throughout the experiment. In three-dimensional experiments, the PLP and FCS groups behaved more congruently, except for the alkaline phosphatase activity and mRNA levels which were markedly increased by PLP.
Human PLP was at least equal to FCS in supporting osteogenic differentiation of human MSCs in two- and three-dimensional conditions; however, proliferation was inferior. As PLP is free of animal components, and thus represents reduced risk for xenogeneic infection, its use for human MSC-induced bone repair in the clinic by the three-dimensional live implants presented here appears a promising therapy option.</description><identifier>ISSN: 1757-6512</identifier><identifier>EISSN: 1757-6512</identifier><identifier>DOI: 10.1186/s13287-015-0162-6</identifier><identifier>PMID: 26345992</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adult ; Analysis ; Cattle ; Cell differentiation ; Cells, Cultured ; Collagen ; Genetic aspects ; Health aspects ; Humans ; Infection ; Mesenchymal Stromal Cells - cytology ; Osteoblasts - cytology ; Osteogenesis ; Phosphatases ; Physiological aspects ; Primary Cell Culture - methods ; Serum ; Stem cells ; Tissue engineering</subject><ispartof>Stem cell research & therapy, 2015-09, Vol.6 (1), p.167-167, Article 167</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2015</rights><rights>Castrén et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c559t-df7f5e2467bd3afe7e6dc4da48133ee877e38050060ffd8a222b9b0a8029a1fe3</citedby><cites>FETCH-LOGICAL-c559t-df7f5e2467bd3afe7e6dc4da48133ee877e38050060ffd8a222b9b0a8029a1fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562352/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562352/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53770,53772</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26345992$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Castrén, Eeva</creatorcontrib><creatorcontrib>Sillat, Tarvo</creatorcontrib><creatorcontrib>Oja, Sofia</creatorcontrib><creatorcontrib>Noro, Ariel</creatorcontrib><creatorcontrib>Laitinen, Anita</creatorcontrib><creatorcontrib>Konttinen, Yrjö T</creatorcontrib><creatorcontrib>Lehenkari, Petri</creatorcontrib><creatorcontrib>Hukkanen, Mika</creatorcontrib><creatorcontrib>Korhonen, Matti</creatorcontrib><title>Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serum</title><title>Stem cell research & therapy</title><addtitle>Stem Cell Res Ther</addtitle><description>Bone marrow-derived mesenchymal stromal cells (MSCs) have been intensely studied for the purpose of developing solutions for clinical tissue engineering. Autologous MSCs can potentially be used to replace tissue defects, but the procedure also carries risks such as immunization and xenogeneic infection. Replacement of the commonly used fetal calf serum (FCS) with human platelet lysate and plasma (PLP) to support cell growth may reduce some of these risks. Altered media could, however, influence stem cell differentiation and we address this experimentally.
We examined human MSC differentiation into the osteoblast lineage using in vitro two- and three-dimensional cultures with PLP or FCS as cell culture medium supplements. Differentiation was followed by quantitative polymerase chain reaction, and alkaline phosphatase activity, matrix formation and matrix calcium content were quantified.
Three-dimensional culture, where human MSCs were grown on collagen sponges, markedly stimulated osteoblast differentiation; a fourfold increase in calcium deposition could be observed in both PLP and FCS groups. PLP-grown cells showed robust osteogenic differentiation both in two- and three-dimensional MSC cultures. The calcium content of the matrix in the two-dimensional PLP group at day 14 was 2.2-fold higher in comparison to the FCS group (p < 0.0001), and at day 21 it was still 1.3-fold higher (p < 0.001), suggesting earlier calcium accumulation to the matrix in the PLP group. This was supported by stronger Alizarin Red staining in the PLP group at day 14. In two-dimesional PLP cultures, cellular proliferation appeared to decrease during later stages of differentiation, while in the FCS group the number of cells increased throughout the experiment. In three-dimensional experiments, the PLP and FCS groups behaved more congruently, except for the alkaline phosphatase activity and mRNA levels which were markedly increased by PLP.
Human PLP was at least equal to FCS in supporting osteogenic differentiation of human MSCs in two- and three-dimensional conditions; however, proliferation was inferior. As PLP is free of animal components, and thus represents reduced risk for xenogeneic infection, its use for human MSC-induced bone repair in the clinic by the three-dimensional live implants presented here appears a promising therapy option.</description><subject>Adult</subject><subject>Analysis</subject><subject>Cattle</subject><subject>Cell differentiation</subject><subject>Cells, Cultured</subject><subject>Collagen</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Infection</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Osteoblasts - cytology</subject><subject>Osteogenesis</subject><subject>Phosphatases</subject><subject>Physiological aspects</subject><subject>Primary Cell Culture - methods</subject><subject>Serum</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><issn>1757-6512</issn><issn>1757-6512</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkttqFTEUhgdRbKl9AG9kQBC9mJrDJJm5EUrxUCgUPFyH7MzKnpSZpObQ2huf3Yy71j1iQlgh-dafZOWvqucYnWDc8bcRU9KJBmFWBicNf1QdYsFEwxkmj_fmB9VxjFeoNEoR4u3T6oBw2rK-J4fVz8uYwG_BWV0P1hgI4JJVyXpXe1PPEMHp8W5WUx1T8EvUME2xtq5Ot74Z7AwuFrpsKDfUaQwAq1Wdp5QDxPrWptHnVDD7Ww5Cnp9VT4yaIhzfx6Pq24f3X88-NReXH8_PTi8azVifmsEIw4C0XGwGqgwI4INuB9V2mFKATgigHWLldciYoVOEkE2_QapDpFfYAD2q3u10r_NmhkGXRwY1yetQrhLupFdWrnecHeXW38iWcUIZKQKv7wWC_54hJjnbuFRCOfA5Siww4ozhvi_oy3_QK59DKcVCCVIu2gn0l9qqCaR1xpdz9SIqT1mLOS0_xwp18h-q9AFmq70DY8v6KuHNKqEwCX6krcoxyvMvn9fsqz12BDWlMfopL58f1yDegTr4GAOYh8JhJBczyp0ZZTGjXMwoecl5sV_xh4w_1qO_AFLB2uI</recordid><startdate>20150907</startdate><enddate>20150907</enddate><creator>Castrén, Eeva</creator><creator>Sillat, Tarvo</creator><creator>Oja, Sofia</creator><creator>Noro, Ariel</creator><creator>Laitinen, Anita</creator><creator>Konttinen, Yrjö T</creator><creator>Lehenkari, Petri</creator><creator>Hukkanen, Mika</creator><creator>Korhonen, Matti</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150907</creationdate><title>Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serum</title><author>Castrén, Eeva ; 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Autologous MSCs can potentially be used to replace tissue defects, but the procedure also carries risks such as immunization and xenogeneic infection. Replacement of the commonly used fetal calf serum (FCS) with human platelet lysate and plasma (PLP) to support cell growth may reduce some of these risks. Altered media could, however, influence stem cell differentiation and we address this experimentally.
We examined human MSC differentiation into the osteoblast lineage using in vitro two- and three-dimensional cultures with PLP or FCS as cell culture medium supplements. Differentiation was followed by quantitative polymerase chain reaction, and alkaline phosphatase activity, matrix formation and matrix calcium content were quantified.
Three-dimensional culture, where human MSCs were grown on collagen sponges, markedly stimulated osteoblast differentiation; a fourfold increase in calcium deposition could be observed in both PLP and FCS groups. PLP-grown cells showed robust osteogenic differentiation both in two- and three-dimensional MSC cultures. The calcium content of the matrix in the two-dimensional PLP group at day 14 was 2.2-fold higher in comparison to the FCS group (p < 0.0001), and at day 21 it was still 1.3-fold higher (p < 0.001), suggesting earlier calcium accumulation to the matrix in the PLP group. This was supported by stronger Alizarin Red staining in the PLP group at day 14. In two-dimesional PLP cultures, cellular proliferation appeared to decrease during later stages of differentiation, while in the FCS group the number of cells increased throughout the experiment. In three-dimensional experiments, the PLP and FCS groups behaved more congruently, except for the alkaline phosphatase activity and mRNA levels which were markedly increased by PLP.
Human PLP was at least equal to FCS in supporting osteogenic differentiation of human MSCs in two- and three-dimensional conditions; however, proliferation was inferior. As PLP is free of animal components, and thus represents reduced risk for xenogeneic infection, its use for human MSC-induced bone repair in the clinic by the three-dimensional live implants presented here appears a promising therapy option.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26345992</pmid><doi>10.1186/s13287-015-0162-6</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Analysis Cattle Cell differentiation Cells, Cultured Collagen Genetic aspects Health aspects Humans Infection Mesenchymal Stromal Cells - cytology Osteoblasts - cytology Osteogenesis Phosphatases Physiological aspects Primary Cell Culture - methods Serum Stem cells Tissue engineering |
| title | Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serum |
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