Separation of Quadruplex Polymorphism in DNA Sequences by Reversed‐Phase Chromatography
This unit describes a method for the separation of a mixture of quadruplex conformations formed from the same parent sequence via reversed‐phase chromatography (RPC). Polymorphism is inherent to quadruplex formation and even relatively simple quadruplex‐forming sequences can fold into a cornucopia o...
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| Veröffentlicht in: | Current Protocols in Nucleic Acid Chemistry 2015-06, Vol.61 (1), p.17.7.1-17.7.18 |
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| container_end_page | 17.7.18 |
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| container_title | Current Protocols in Nucleic Acid Chemistry |
| container_volume | 61 |
| creator | Miller, M. Clarke Ohrenberg, Carl J. Kuttan, Ashani Trent, John O. |
| description | This unit describes a method for the separation of a mixture of quadruplex conformations formed from the same parent sequence via reversed‐phase chromatography (RPC). Polymorphism is inherent to quadruplex formation and even relatively simple quadruplex‐forming sequences can fold into a cornucopia of possible conformations and topologies. Isolation of a specific conformation for study can be problematic. This is especially true for conformations of the human telomere sequence d(GGG(TTAGGG)3). High performance liquid chromatography (HPLC), especially reversed‐phase chromatography, has been a mainstay of nucleic acid research and purification for many decades. We have successfully applied this method to the problem of separating individual quadruplex species in the ensemble from the same parent sequence. © 2015 by John Wiley & Sons, Inc. |
| doi_str_mv | 10.1002/0471142700.nc1707s61 |
| format | Article |
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We have successfully applied this method to the problem of separating individual quadruplex species in the ensemble from the same parent sequence. © 2015 by John Wiley & Sons, Inc.</description><identifier>ISSN: 1934-9270</identifier><identifier>EISSN: 1934-9289</identifier><identifier>DOI: 10.1002/0471142700.nc1707s61</identifier><identifier>PMID: 26344226</identifier><language>eng</language><publisher>United States</publisher><subject>Chromatography, Reverse-Phase - methods ; DNA - genetics ; G-Quadruplexes ; Humans ; Polymerase Chain Reaction ; quadruplex ; reversed‐phase chromatography ; RPC ; telomere ; Telomere - genetics</subject><ispartof>Current Protocols in Nucleic Acid Chemistry, 2015-06, Vol.61 (1), p.17.7.1-17.7.18</ispartof><rights>Copyright © 2015 John Wiley & Sons, Inc.</rights><rights>Copyright © 2013 John Wiley & Sons, Inc. 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Clarke</creatorcontrib><creatorcontrib>Ohrenberg, Carl J.</creatorcontrib><creatorcontrib>Kuttan, Ashani</creatorcontrib><creatorcontrib>Trent, John O.</creatorcontrib><title>Separation of Quadruplex Polymorphism in DNA Sequences by Reversed‐Phase Chromatography</title><title>Current Protocols in Nucleic Acid Chemistry</title><addtitle>Curr Protoc Nucleic Acid Chem</addtitle><description>This unit describes a method for the separation of a mixture of quadruplex conformations formed from the same parent sequence via reversed‐phase chromatography (RPC). Polymorphism is inherent to quadruplex formation and even relatively simple quadruplex‐forming sequences can fold into a cornucopia of possible conformations and topologies. Isolation of a specific conformation for study can be problematic. This is especially true for conformations of the human telomere sequence d(GGG(TTAGGG)3). High performance liquid chromatography (HPLC), especially reversed‐phase chromatography, has been a mainstay of nucleic acid research and purification for many decades. We have successfully applied this method to the problem of separating individual quadruplex species in the ensemble from the same parent sequence. © 2015 by John Wiley & Sons, Inc.</description><subject>Chromatography, Reverse-Phase - methods</subject><subject>DNA - genetics</subject><subject>G-Quadruplexes</subject><subject>Humans</subject><subject>Polymerase Chain Reaction</subject><subject>quadruplex</subject><subject>reversed‐phase chromatography</subject><subject>RPC</subject><subject>telomere</subject><subject>Telomere - genetics</subject><issn>1934-9270</issn><issn>1934-9289</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1KxEAQhRtRVNQbiPQFZuy_pCcuhCH-guj4t3DVdDoVE0jSsXsymp1H8IyexMho1J2Logree1_BQ2iXkjElhO0TISkVTBIyrg2VRPqQrqBNGnExitgkWh1uSTbQjvdFQggnPJpEwTraYCEXgrFwEz3cQqOdnhe2xjbD161OXduU8IJntuwq65q88BUuanx0OcW38NRCbcDjpMM3sADnIX1_fZvl2gOOc2crPbePTjd5t43WMl162PnaW-j-5PguPhtdXJ2ex9OLkeFSyJEwLBBCmCQJQaYiYBFo0o_OSJYlGeWMGBJmQHkvmaB3gIlCzoFTFkxoyLfQ4ZLbtEkFqYF67nSpGldU2nXK6kL9VeoiV492oUQQ0kkge4BYAoyz3jvIhiwl6rNt9dO2GtruY3u__w6h7257w8HS8FyU0P0LquLZZfx58w8Tqo-H</recordid><startdate>201506</startdate><enddate>201506</enddate><creator>Miller, M. 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| identifier | ISSN: 1934-9270 |
| ispartof | Current Protocols in Nucleic Acid Chemistry, 2015-06, Vol.61 (1), p.17.7.1-17.7.18 |
| issn | 1934-9270 1934-9289 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Chromatography, Reverse-Phase - methods DNA - genetics G-Quadruplexes Humans Polymerase Chain Reaction quadruplex reversed‐phase chromatography RPC telomere Telomere - genetics |
| title | Separation of Quadruplex Polymorphism in DNA Sequences by Reversed‐Phase Chromatography |
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