Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design
Monkeypox virus (MPXV) infection of the prairie dog is valuable to studying systemic orthopoxvirus disease. To further characterize differences in MPXV clade pathogenesis, groups of prairie dogs were intranasally infected (8×103 p.f.u.) with Congo Basin (CB) or West African (WA) MPXV, and 28 tissues...
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| creator | Sanders, Jeanine Smith, Scott K. Keckler, M. Shannon Karem, Kevin Damon, Inger K. Olson, Victoria A. Hughes, Christine Nagy, Tamas Zaki, Sherif R. Drew, Clifton Gallardo-Romero, Nadia Carroll, Darin S. Hutson, Christina L. Patel, Nishi |
| description | Monkeypox virus (MPXV) infection of the prairie dog is valuable to studying systemic orthopoxvirus disease. To further characterize differences in MPXV clade pathogenesis, groups of prairie dogs were intranasally infected (8×103 p.f.u.) with Congo Basin (CB) or West African (WA) MPXV, and 28 tissues were harvested on days 2, 4, 6, 9, 12, 17, and 24 postinfection. Samples were evaluated for the presence of virus and gross and microscopic lesions. Virus was recovered from nasal mucosa, oropharyngeal lymph nodes, and spleen earlier in CB challenged animals (day 4) than WA challenged animals (day 6). For both groups, primary viremia (indicated by viral DNA) was seen on days 6–9 through day 17. CB MPXV spread more rapidly, accumulated to greater levels, and caused greater morbidity in animals compared to WA MPXV. Histopathology and immunohistochemistry (IHC) findings, however, were similar. Two animals that succumbed to disease demonstrated abundant viral antigen in all organs tested, except for brain. Dual-IHC staining of select liver and spleen sections showed that apoptotic cells (identified by TUNEL) tended to colocalize with poxvirus antigen. Interestingly splenocytes were labelled positive for apoptosis more often than hepatocytes in both MPXV groups. These findings allow for further characterization of differences between MPXV clade pathogenesis, including identifying sites that are important during early viral replication and cellular response to viral infection. |
| doi_str_mv | 10.1155/2015/965710 |
| format | Article |
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Shannon ; Karem, Kevin ; Damon, Inger K. ; Olson, Victoria A. ; Hughes, Christine ; Nagy, Tamas ; Zaki, Sherif R. ; Drew, Clifton ; Gallardo-Romero, Nadia ; Carroll, Darin S. ; Hutson, Christina L. ; Patel, Nishi</creator><contributor>Qin, Cheng-Feng</contributor><creatorcontrib>Sanders, Jeanine ; Smith, Scott K. ; Keckler, M. Shannon ; Karem, Kevin ; Damon, Inger K. ; Olson, Victoria A. ; Hughes, Christine ; Nagy, Tamas ; Zaki, Sherif R. ; Drew, Clifton ; Gallardo-Romero, Nadia ; Carroll, Darin S. ; Hutson, Christina L. ; Patel, Nishi ; Qin, Cheng-Feng</creatorcontrib><description>Monkeypox virus (MPXV) infection of the prairie dog is valuable to studying systemic orthopoxvirus disease. To further characterize differences in MPXV clade pathogenesis, groups of prairie dogs were intranasally infected (8×103 p.f.u.) with Congo Basin (CB) or West African (WA) MPXV, and 28 tissues were harvested on days 2, 4, 6, 9, 12, 17, and 24 postinfection. Samples were evaluated for the presence of virus and gross and microscopic lesions. Virus was recovered from nasal mucosa, oropharyngeal lymph nodes, and spleen earlier in CB challenged animals (day 4) than WA challenged animals (day 6). For both groups, primary viremia (indicated by viral DNA) was seen on days 6–9 through day 17. CB MPXV spread more rapidly, accumulated to greater levels, and caused greater morbidity in animals compared to WA MPXV. Histopathology and immunohistochemistry (IHC) findings, however, were similar. Two animals that succumbed to disease demonstrated abundant viral antigen in all organs tested, except for brain. Dual-IHC staining of select liver and spleen sections showed that apoptotic cells (identified by TUNEL) tended to colocalize with poxvirus antigen. Interestingly splenocytes were labelled positive for apoptosis more often than hepatocytes in both MPXV groups. These findings allow for further characterization of differences between MPXV clade pathogenesis, including identifying sites that are important during early viral replication and cellular response to viral infection.</description><identifier>ISSN: 2314-6133</identifier><identifier>EISSN: 2314-6141</identifier><identifier>DOI: 10.1155/2015/965710</identifier><identifier>PMID: 26380309</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Animals ; Disease Models, Animal ; DNA, Viral - blood ; DNA, Viral - genetics ; Health aspects ; Human monkeypox ; Infections ; Kinetics ; Liver - virology ; Lymph Nodes - virology ; Monkeypox virus ; Monkeypox virus - genetics ; Monkeypox virus - pathogenicity ; Mpox ; Mpox (monkeypox) - blood ; Mpox (monkeypox) - genetics ; Mpox (monkeypox) - pathology ; Mpox (monkeypox) - virology ; Nasal Mucosa - virology ; Orthopoxvirus ; Pathogenesis ; Pathology ; Phylogeny ; Poxvirus ; Prairie dogs ; Rodents ; Sciuridae - blood ; Sciuridae - genetics ; Sciuridae - virology ; Spleen - virology ; Studies ; Viral infections ; Virus Replication - genetics</subject><ispartof>BioMed research international, 2015-01, Vol.2015 (2015), p.1-19</ispartof><rights>Copyright © 2015 Christina L. Hutson et al.</rights><rights>COPYRIGHT 2015 John Wiley & Sons, Inc.</rights><rights>Copyright © 2015 Christina L. Hutson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2015 Christina L. Hutson et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-6c16b2b9b8b271c6517025e028605a1806943b6926aba13284b12e86f7bf82713</citedby><cites>FETCH-LOGICAL-c528t-6c16b2b9b8b271c6517025e028605a1806943b6926aba13284b12e86f7bf82713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4561332/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4561332/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,725,778,782,883,27913,27914,53780,53782</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26380309$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Qin, Cheng-Feng</contributor><creatorcontrib>Sanders, Jeanine</creatorcontrib><creatorcontrib>Smith, Scott K.</creatorcontrib><creatorcontrib>Keckler, M. Shannon</creatorcontrib><creatorcontrib>Karem, Kevin</creatorcontrib><creatorcontrib>Damon, Inger K.</creatorcontrib><creatorcontrib>Olson, Victoria A.</creatorcontrib><creatorcontrib>Hughes, Christine</creatorcontrib><creatorcontrib>Nagy, Tamas</creatorcontrib><creatorcontrib>Zaki, Sherif R.</creatorcontrib><creatorcontrib>Drew, Clifton</creatorcontrib><creatorcontrib>Gallardo-Romero, Nadia</creatorcontrib><creatorcontrib>Carroll, Darin S.</creatorcontrib><creatorcontrib>Hutson, Christina L.</creatorcontrib><creatorcontrib>Patel, Nishi</creatorcontrib><title>Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design</title><title>BioMed research international</title><addtitle>Biomed Res Int</addtitle><description>Monkeypox virus (MPXV) infection of the prairie dog is valuable to studying systemic orthopoxvirus disease. 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Dual-IHC staining of select liver and spleen sections showed that apoptotic cells (identified by TUNEL) tended to colocalize with poxvirus antigen. Interestingly splenocytes were labelled positive for apoptosis more often than hepatocytes in both MPXV groups. These findings allow for further characterization of differences between MPXV clade pathogenesis, including identifying sites that are important during early viral replication and cellular response to viral infection.</description><subject>Animals</subject><subject>Disease Models, Animal</subject><subject>DNA, Viral - blood</subject><subject>DNA, Viral - genetics</subject><subject>Health aspects</subject><subject>Human monkeypox</subject><subject>Infections</subject><subject>Kinetics</subject><subject>Liver - virology</subject><subject>Lymph Nodes - virology</subject><subject>Monkeypox virus</subject><subject>Monkeypox virus - genetics</subject><subject>Monkeypox virus - pathogenicity</subject><subject>Mpox</subject><subject>Mpox (monkeypox) - blood</subject><subject>Mpox (monkeypox) - genetics</subject><subject>Mpox (monkeypox) - pathology</subject><subject>Mpox (monkeypox) - virology</subject><subject>Nasal Mucosa - virology</subject><subject>Orthopoxvirus</subject><subject>Pathogenesis</subject><subject>Pathology</subject><subject>Phylogeny</subject><subject>Poxvirus</subject><subject>Prairie dogs</subject><subject>Rodents</subject><subject>Sciuridae - blood</subject><subject>Sciuridae - genetics</subject><subject>Sciuridae - virology</subject><subject>Spleen - virology</subject><subject>Studies</subject><subject>Viral infections</subject><subject>Virus Replication - genetics</subject><issn>2314-6133</issn><issn>2314-6141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNks9vFCEcxYnR2Gbtybsh8WI0a_kxMHAx2Wz9FWtsstYrYWa-M0udhS3MWPfuHy6TrWv1VC4Q-PAC7z2EnlLymlIhThmh4lRLUVLyAB0zTou5pAV9eFhzfoROUroieSgqiZaP0RGTXBFO9DH6tQybrY0uBY9Diz8H_x122_ATf3NxTHjZ2wbwJ-dhcHXC1jf4wg7r0Iduh2_csHYeD2vAF9G66ACfhQ4vvNvYPks10OPL5HyHLV5BdHlzZevoWlcDXg1js8NnkFznn6BHre0TnNzOM3T57u3X5Yf5-Zf3H5eL83ktmBrmsqayYpWuVMVKWktBS8IEEKYkEZYqInXBK6mZtJWlnKmiogyUbMuqVfkGn6E3e93tWG2gqcEP0fZmG_OD484E68y_J96tTRd-mEJMRrIs8OJWIIbrEdJgNi7V0PfWQxiToSVjtNSEq3uglOui1GxSff4fehXG6LMTmcqBaaaZ-Et1tgfjfBvyE-tJ1CwKkaMtSI51hl7tqTqGlCK0h99RYqbGmKkxZt-YTD-7a8iB_dOPDLzcAznoxt64-6lBRqC1d2BRFtnA3xwqz10</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Sanders, Jeanine</creator><creator>Smith, Scott K.</creator><creator>Keckler, M. 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Shannon</au><au>Karem, Kevin</au><au>Damon, Inger K.</au><au>Olson, Victoria A.</au><au>Hughes, Christine</au><au>Nagy, Tamas</au><au>Zaki, Sherif R.</au><au>Drew, Clifton</au><au>Gallardo-Romero, Nadia</au><au>Carroll, Darin S.</au><au>Hutson, Christina L.</au><au>Patel, Nishi</au><au>Qin, Cheng-Feng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design</atitle><jtitle>BioMed research international</jtitle><addtitle>Biomed Res Int</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>2015</volume><issue>2015</issue><spage>1</spage><epage>19</epage><pages>1-19</pages><issn>2314-6133</issn><eissn>2314-6141</eissn><abstract>Monkeypox virus (MPXV) infection of the prairie dog is valuable to studying systemic orthopoxvirus disease. To further characterize differences in MPXV clade pathogenesis, groups of prairie dogs were intranasally infected (8×103 p.f.u.) with Congo Basin (CB) or West African (WA) MPXV, and 28 tissues were harvested on days 2, 4, 6, 9, 12, 17, and 24 postinfection. Samples were evaluated for the presence of virus and gross and microscopic lesions. Virus was recovered from nasal mucosa, oropharyngeal lymph nodes, and spleen earlier in CB challenged animals (day 4) than WA challenged animals (day 6). For both groups, primary viremia (indicated by viral DNA) was seen on days 6–9 through day 17. CB MPXV spread more rapidly, accumulated to greater levels, and caused greater morbidity in animals compared to WA MPXV. Histopathology and immunohistochemistry (IHC) findings, however, were similar. Two animals that succumbed to disease demonstrated abundant viral antigen in all organs tested, except for brain. Dual-IHC staining of select liver and spleen sections showed that apoptotic cells (identified by TUNEL) tended to colocalize with poxvirus antigen. Interestingly splenocytes were labelled positive for apoptosis more often than hepatocytes in both MPXV groups. These findings allow for further characterization of differences between MPXV clade pathogenesis, including identifying sites that are important during early viral replication and cellular response to viral infection.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>26380309</pmid><doi>10.1155/2015/965710</doi><tpages>19</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2314-6133 |
| ispartof | BioMed research international, 2015-01, Vol.2015 (2015), p.1-19 |
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| language | eng |
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| source | MEDLINE; PubMed Central Open Access; Wiley Online Library Open Access; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Disease Models, Animal DNA, Viral - blood DNA, Viral - genetics Health aspects Human monkeypox Infections Kinetics Liver - virology Lymph Nodes - virology Monkeypox virus Monkeypox virus - genetics Monkeypox virus - pathogenicity Mpox Mpox (monkeypox) - blood Mpox (monkeypox) - genetics Mpox (monkeypox) - pathology Mpox (monkeypox) - virology Nasal Mucosa - virology Orthopoxvirus Pathogenesis Pathology Phylogeny Poxvirus Prairie dogs Rodents Sciuridae - blood Sciuridae - genetics Sciuridae - virology Spleen - virology Studies Viral infections Virus Replication - genetics |
| title | Comparison of Monkeypox Virus Clade Kinetics and Pathology within the Prairie Dog Animal Model Using a Serial Sacrifice Study Design |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T08%3A34%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20Monkeypox%20Virus%20Clade%20Kinetics%20and%20Pathology%20within%20the%20Prairie%20Dog%20Animal%20Model%20Using%20a%20Serial%20Sacrifice%20Study%20Design&rft.jtitle=BioMed%20research%20international&rft.au=Sanders,%20Jeanine&rft.date=2015-01-01&rft.volume=2015&rft.issue=2015&rft.spage=1&rft.epage=19&rft.pages=1-19&rft.issn=2314-6133&rft.eissn=2314-6141&rft_id=info:doi/10.1155/2015/965710&rft_dat=%3Cgale_pubme%3EA458164026%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1709692925&rft_id=info:pmid/26380309&rft_galeid=A458164026&rfr_iscdi=true |