Purification of monoclonal antibodies from clarified cell culture fluid using Protein A capture continuous countercurrent tangential chromatography

Recent studies using simple model systems have demonstrated that continuous countercurrent tangential chromatography (CCTC) has the potential to overcome many of the limitations of conventional Protein A chromatography using packed columns. The objective of this work was to optimize and implement a...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of biotechnology 2015-11, Vol.213, p.54-64
Hauptverfasser:	Dutta, Amit K., Tran, Travis, Napadensky, Boris, Teella, Achyuta, Brookhart, Gary, Ropp, Philip A., Zhang, Ada W., Tustian, Andrew D., Zydney, Andrew L., Shinkazh, Oleg
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - isolation & purification Binding Biotechnology cell culture Cell Culture Techniques Chinese hamsters CHO Cells Chromatography Chromatography - methods Continuous Cricetinae Cricetulus Monoclonal antibodies Monoclonal antibody Productivity Protein A pumps Purification slurries Staphylococcal Protein A - chemistry
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!