Effects of magnetic nanoparticles of Fe3O4 combinated with gambogic acid on apoptosis of SMMC-7721 cells
This study aims to investigate the potential benefit of combination therapy with magnetic nanoparticles of Fe3O4 (Fe3O4-MNP) and gambogic acid (GA) on SMMC-7721 cells. The inhibition of proliferation of SMMC-7721 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ass...
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| Veröffentlicht in: | OncoTargets and therapy 2015-01, Vol.8, p.2285-2290 |
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| creator | Tian, Liang Chen, Bao-An Cheng, Jian Guo, Qing-Long |
| description | This study aims to investigate the potential benefit of combination therapy with magnetic nanoparticles of Fe3O4 (Fe3O4-MNP) and gambogic acid (GA) on SMMC-7721 cells.
The inhibition of proliferation of SMMC-7721 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was calculated and analyzed by flow cytometry, and the expressions of the apoptosis-related protein were detected by Western blot.
GA enhanced the cytotoxicity of SMMC-7721 cells in a dose-dependent manner. The Fe3O4-MNP itself had no obviously inhibitory effect, but it could enhance the effect of GA on proliferation of SMMC-7721 cells. The apoptotic rate of SMMC-7721 cells induced by combination of GA with Fe3O4-MNP was higher than that by GA alone. The expression levels of caspase-3 and caspase-8 after co-treatment of GA and Fe3O4-MNP were higher than that exposed to either GA or Fe3O4-MNP alone, while the levels of bcl-2 were downregulated.
Fe3O4-MNP can promote GA-induced apoptosis of SMMC-7721 cells, which may be related to the downregulation of Bcl-2 and upregulation of caspase-3. |
| doi_str_mv | 10.2147/OTT.S86494 |
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The inhibition of proliferation of SMMC-7721 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was calculated and analyzed by flow cytometry, and the expressions of the apoptosis-related protein were detected by Western blot.
GA enhanced the cytotoxicity of SMMC-7721 cells in a dose-dependent manner. The Fe3O4-MNP itself had no obviously inhibitory effect, but it could enhance the effect of GA on proliferation of SMMC-7721 cells. The apoptotic rate of SMMC-7721 cells induced by combination of GA with Fe3O4-MNP was higher than that by GA alone. The expression levels of caspase-3 and caspase-8 after co-treatment of GA and Fe3O4-MNP were higher than that exposed to either GA or Fe3O4-MNP alone, while the levels of bcl-2 were downregulated.
Fe3O4-MNP can promote GA-induced apoptosis of SMMC-7721 cells, which may be related to the downregulation of Bcl-2 and upregulation of caspase-3.</description><identifier>ISSN: 1178-6930</identifier><identifier>EISSN: 1178-6930</identifier><identifier>DOI: 10.2147/OTT.S86494</identifier><identifier>PMID: 26345420</identifier><language>eng</language><publisher>New Zealand: Taylor & Francis Ltd</publisher><subject>Acids ; Apoptosis ; Autophagy ; Biotechnology ; Cancer therapies ; Chinese medicine ; Colorectal cancer ; Drug dosages ; Experiments ; Flow cytometry ; Hepatitis ; In vitro fertilization ; Laboratories ; Liver cancer ; Lung cancer ; Lymphoma ; Nanoparticles ; Original Research ; Proteins ; Variance analysis</subject><ispartof>OncoTargets and therapy, 2015-01, Vol.8, p.2285-2290</ispartof><rights>2015. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2015 Tian et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-787e4cc043a53af870fbc5f0efe2d407cede14fd7ee5a05258864c17e33295573</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556044/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556044/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,3848,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26345420$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tian, Liang</creatorcontrib><creatorcontrib>Chen, Bao-An</creatorcontrib><creatorcontrib>Cheng, Jian</creatorcontrib><creatorcontrib>Guo, Qing-Long</creatorcontrib><title>Effects of magnetic nanoparticles of Fe3O4 combinated with gambogic acid on apoptosis of SMMC-7721 cells</title><title>OncoTargets and therapy</title><addtitle>Onco Targets Ther</addtitle><description>This study aims to investigate the potential benefit of combination therapy with magnetic nanoparticles of Fe3O4 (Fe3O4-MNP) and gambogic acid (GA) on SMMC-7721 cells.
The inhibition of proliferation of SMMC-7721 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was calculated and analyzed by flow cytometry, and the expressions of the apoptosis-related protein were detected by Western blot.
GA enhanced the cytotoxicity of SMMC-7721 cells in a dose-dependent manner. The Fe3O4-MNP itself had no obviously inhibitory effect, but it could enhance the effect of GA on proliferation of SMMC-7721 cells. The apoptotic rate of SMMC-7721 cells induced by combination of GA with Fe3O4-MNP was higher than that by GA alone. The expression levels of caspase-3 and caspase-8 after co-treatment of GA and Fe3O4-MNP were higher than that exposed to either GA or Fe3O4-MNP alone, while the levels of bcl-2 were downregulated.
Fe3O4-MNP can promote GA-induced apoptosis of SMMC-7721 cells, which may be related to the downregulation of Bcl-2 and upregulation of caspase-3.</description><subject>Acids</subject><subject>Apoptosis</subject><subject>Autophagy</subject><subject>Biotechnology</subject><subject>Cancer therapies</subject><subject>Chinese medicine</subject><subject>Colorectal cancer</subject><subject>Drug dosages</subject><subject>Experiments</subject><subject>Flow cytometry</subject><subject>Hepatitis</subject><subject>In vitro fertilization</subject><subject>Laboratories</subject><subject>Liver cancer</subject><subject>Lung cancer</subject><subject>Lymphoma</subject><subject>Nanoparticles</subject><subject>Original Research</subject><subject>Proteins</subject><subject>Variance analysis</subject><issn>1178-6930</issn><issn>1178-6930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkUtLBDEQhIMovi_-AAl4EWE0z8nsRZDFFyh7cD2HbKazG5lJxsms4r83uirqqQv666KLQuiAklNGhTqbTKenD1UpRmINbVOqqqIccbL-S2-hnZSeCCnLiolNtMVKLqRgZBstLp0DOyQcHW7NPMDgLQ4mxM70WTbwubkCPhHYxnbmgxmgxq9-WOC5aWdxnnljfY1jwKaL3RCT_7x5uL8fF0oxii00TdpDG840Cfa_5i56vLqcjm-Ku8n17fjirrBckaFQlQJhLRHcSG5cpYibWekIOGC1IMpCDVS4WgFIQySTVQ5uqQLO2UhKxXfR-cq3W85aqC2EoTeN7nrfmv5NR-P1303wCz2PL1pIWRIhssHxl0Efn5eQBt369BHBBIjLpKmipJQVJ1VGj_6hT3HZhxxPMyYYI4oqnqmTFWX7mFIP7ucZSvRHgToXqFcFZvjw9_s_6Hdj_B1Cj5Wp</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Tian, Liang</creator><creator>Chen, Bao-An</creator><creator>Cheng, Jian</creator><creator>Guo, Qing-Long</creator><general>Taylor & Francis Ltd</general><general>Dove Medical Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>Effects of magnetic nanoparticles of Fe3O4 combinated with gambogic acid on apoptosis of SMMC-7721 cells</title><author>Tian, Liang ; Chen, Bao-An ; Cheng, Jian ; Guo, Qing-Long</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-787e4cc043a53af870fbc5f0efe2d407cede14fd7ee5a05258864c17e33295573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acids</topic><topic>Apoptosis</topic><topic>Autophagy</topic><topic>Biotechnology</topic><topic>Cancer therapies</topic><topic>Chinese medicine</topic><topic>Colorectal cancer</topic><topic>Drug dosages</topic><topic>Experiments</topic><topic>Flow cytometry</topic><topic>Hepatitis</topic><topic>In vitro fertilization</topic><topic>Laboratories</topic><topic>Liver cancer</topic><topic>Lung cancer</topic><topic>Lymphoma</topic><topic>Nanoparticles</topic><topic>Original Research</topic><topic>Proteins</topic><topic>Variance analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tian, Liang</creatorcontrib><creatorcontrib>Chen, Bao-An</creatorcontrib><creatorcontrib>Cheng, Jian</creatorcontrib><creatorcontrib>Guo, Qing-Long</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>OncoTargets and therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tian, Liang</au><au>Chen, Bao-An</au><au>Cheng, Jian</au><au>Guo, Qing-Long</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of magnetic nanoparticles of Fe3O4 combinated with gambogic acid on apoptosis of SMMC-7721 cells</atitle><jtitle>OncoTargets and therapy</jtitle><addtitle>Onco Targets Ther</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>8</volume><spage>2285</spage><epage>2290</epage><pages>2285-2290</pages><issn>1178-6930</issn><eissn>1178-6930</eissn><abstract>This study aims to investigate the potential benefit of combination therapy with magnetic nanoparticles of Fe3O4 (Fe3O4-MNP) and gambogic acid (GA) on SMMC-7721 cells.
The inhibition of proliferation of SMMC-7721 cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was calculated and analyzed by flow cytometry, and the expressions of the apoptosis-related protein were detected by Western blot.
GA enhanced the cytotoxicity of SMMC-7721 cells in a dose-dependent manner. The Fe3O4-MNP itself had no obviously inhibitory effect, but it could enhance the effect of GA on proliferation of SMMC-7721 cells. The apoptotic rate of SMMC-7721 cells induced by combination of GA with Fe3O4-MNP was higher than that by GA alone. The expression levels of caspase-3 and caspase-8 after co-treatment of GA and Fe3O4-MNP were higher than that exposed to either GA or Fe3O4-MNP alone, while the levels of bcl-2 were downregulated.
Fe3O4-MNP can promote GA-induced apoptosis of SMMC-7721 cells, which may be related to the downregulation of Bcl-2 and upregulation of caspase-3.</abstract><cop>New Zealand</cop><pub>Taylor & Francis Ltd</pub><pmid>26345420</pmid><doi>10.2147/OTT.S86494</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Taylor & Francis Open Access; DOVE Medical Press Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Acids Apoptosis Autophagy Biotechnology Cancer therapies Chinese medicine Colorectal cancer Drug dosages Experiments Flow cytometry Hepatitis In vitro fertilization Laboratories Liver cancer Lung cancer Lymphoma Nanoparticles Original Research Proteins Variance analysis |
| title | Effects of magnetic nanoparticles of Fe3O4 combinated with gambogic acid on apoptosis of SMMC-7721 cells |
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