Detection of theileriosis in cattle and buffaloes by polymerase chain reaction
Bovine tropical theileriosis caused by Theileria annulata is a tick-borne disease of great economic importance in tropical and subtropical regions of the world. The present study was undertaken to detect theilerosis in cattle and buffaloes by polymerase chain reaction (PCR). The diagnosis of theiler...
Gespeichert in:
| Veröffentlicht in: | Journal of parasitic diseases 2015-09, Vol.39 (3), p.508-513 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 513 |
|---|---|
| container_issue | 3 |
| container_start_page | 508 |
| container_title | Journal of parasitic diseases |
| container_volume | 39 |
| creator | Kundave, V. R. Patel, A. K. Patel, P. V. Hasnani, J. J. Joshi, C. G. |
| description | Bovine tropical theileriosis caused by Theileria annulata is a tick-borne disease of great economic importance in tropical and subtropical regions of the world. The present study was undertaken to detect theilerosis in cattle and buffaloes by polymerase chain reaction (PCR). The diagnosis of theileriosis is usually carried out by blood smear staining technique, which is not sufficiently sensitive to detect the piroplasms in the carrier animals. In this study, a total of 116 samples were collected from infected as well as apparently healthy cattle and buffaloes. Screening of blood smears by Giemsa staining detected 15 samples (12.93 %) positive for Theileria piroplasms out of 116 samples. However, the PCR based screening using the specific primers from the major merozoite-piroplasm surface antigen sequence of T. annulata (Tams1) gene detected 74 samples (63.79 %) positive for T. annulata which included 59 samples found negative by Giemsa staining. Our study suggests that the PCR based screening is more sensitive and accurate method for diagnosis of tropical theileriosis in cattle and buffaloes. |
| doi_str_mv | 10.1007/s12639-013-0386-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4554556</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1710657430</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5782-7bb06b5b211f5f905976e0a6cc5741976ede6fe77e67138efeb6b1f056df36013</originalsourceid><addsrcrecordid>eNqFkU2PFCEQhonRuB_6A7woiRcvrUXTFN0XE7O6arLRg-6ZQE8xw6anGaHbZP69zPS6fhw0gfBRz_tSRTH2RMBLAaBfZVGj7CoQsgLZYlXfY6fQaVWBBnn_uBeVFh2esLOcbwBUuW8fspMiaxSgOGWf3tJE_RTiyKPn04bCQCnEHDIPI-_tNA3E7bjibvbeDpEyd3u-i8N-S8lm4v3GFjCRPZo8Yg8Klenx7XrOri_ffb34UF19fv_x4s1V1Svd1pV2DtApVwvhle9AdRoJLPYl3IjDYUXoSWtCLWRLnhw64UHhykss9Z6z14vvbnZbWvU0TskOZpfC1qa9iTaYPyNj2Jh1_G4apcrAYvDi1iDFbzPlyWxD7mkY7Ehxzka05YdRSNX-H9UCsOQtoaDP_0Jv4pzG8hOmbqCRHcLRUCxUn2LOifxd3gLMobFmaawphZpDY01dNE9_L_hO8bOTBagXIJfQuKb06-l_uT5bRN5GY9cpZHP9pQaBUKYEjfIHzMm26g</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2404396058</pqid></control><display><type>article</type><title>Detection of theileriosis in cattle and buffaloes by polymerase chain reaction</title><source>SpringerLink Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Kundave, V. R. ; Patel, A. K. ; Patel, P. V. ; Hasnani, J. J. ; Joshi, C. G.</creator><creatorcontrib>Kundave, V. R. ; Patel, A. K. ; Patel, P. V. ; Hasnani, J. J. ; Joshi, C. G.</creatorcontrib><description>Bovine tropical theileriosis caused by Theileria annulata is a tick-borne disease of great economic importance in tropical and subtropical regions of the world. The present study was undertaken to detect theilerosis in cattle and buffaloes by polymerase chain reaction (PCR). The diagnosis of theileriosis is usually carried out by blood smear staining technique, which is not sufficiently sensitive to detect the piroplasms in the carrier animals. In this study, a total of 116 samples were collected from infected as well as apparently healthy cattle and buffaloes. Screening of blood smears by Giemsa staining detected 15 samples (12.93 %) positive for Theileria piroplasms out of 116 samples. However, the PCR based screening using the specific primers from the major merozoite-piroplasm surface antigen sequence of T. annulata (Tams1) gene detected 74 samples (63.79 %) positive for T. annulata which included 59 samples found negative by Giemsa staining. Our study suggests that the PCR based screening is more sensitive and accurate method for diagnosis of tropical theileriosis in cattle and buffaloes.</description><identifier>ISSN: 0971-7196</identifier><identifier>EISSN: 0975-0703</identifier><identifier>DOI: 10.1007/s12639-013-0386-2</identifier><identifier>PMID: 26345061</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>blood ; buffaloes ; Cattle ; Diagnosis ; Economic importance ; genes ; Health Promotion and Disease Prevention ; Infectious Diseases ; Medicine ; Medicine & Public Health ; Original ; Original Article ; Polymerase chain reaction ; screening ; staining ; subtropics ; surface antigens ; Theileria annulata ; Theileriosis ; Tick-borne diseases</subject><ispartof>Journal of parasitic diseases, 2015-09, Vol.39 (3), p.508-513</ispartof><rights>Indian Society for Parasitology 2013</rights><rights>Indian Society for Parasitology 2013.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5782-7bb06b5b211f5f905976e0a6cc5741976ede6fe77e67138efeb6b1f056df36013</citedby><cites>FETCH-LOGICAL-c5782-7bb06b5b211f5f905976e0a6cc5741976ede6fe77e67138efeb6b1f056df36013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4554556/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4554556/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26345061$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kundave, V. R.</creatorcontrib><creatorcontrib>Patel, A. K.</creatorcontrib><creatorcontrib>Patel, P. V.</creatorcontrib><creatorcontrib>Hasnani, J. J.</creatorcontrib><creatorcontrib>Joshi, C. G.</creatorcontrib><title>Detection of theileriosis in cattle and buffaloes by polymerase chain reaction</title><title>Journal of parasitic diseases</title><addtitle>J Parasit Dis</addtitle><addtitle>J Parasit Dis</addtitle><description>Bovine tropical theileriosis caused by Theileria annulata is a tick-borne disease of great economic importance in tropical and subtropical regions of the world. The present study was undertaken to detect theilerosis in cattle and buffaloes by polymerase chain reaction (PCR). The diagnosis of theileriosis is usually carried out by blood smear staining technique, which is not sufficiently sensitive to detect the piroplasms in the carrier animals. In this study, a total of 116 samples were collected from infected as well as apparently healthy cattle and buffaloes. Screening of blood smears by Giemsa staining detected 15 samples (12.93 %) positive for Theileria piroplasms out of 116 samples. However, the PCR based screening using the specific primers from the major merozoite-piroplasm surface antigen sequence of T. annulata (Tams1) gene detected 74 samples (63.79 %) positive for T. annulata which included 59 samples found negative by Giemsa staining. Our study suggests that the PCR based screening is more sensitive and accurate method for diagnosis of tropical theileriosis in cattle and buffaloes.</description><subject>blood</subject><subject>buffaloes</subject><subject>Cattle</subject><subject>Diagnosis</subject><subject>Economic importance</subject><subject>genes</subject><subject>Health Promotion and Disease Prevention</subject><subject>Infectious Diseases</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Original</subject><subject>Original Article</subject><subject>Polymerase chain reaction</subject><subject>screening</subject><subject>staining</subject><subject>subtropics</subject><subject>surface antigens</subject><subject>Theileria annulata</subject><subject>Theileriosis</subject><subject>Tick-borne diseases</subject><issn>0971-7196</issn><issn>0975-0703</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqFkU2PFCEQhonRuB_6A7woiRcvrUXTFN0XE7O6arLRg-6ZQE8xw6anGaHbZP69zPS6fhw0gfBRz_tSRTH2RMBLAaBfZVGj7CoQsgLZYlXfY6fQaVWBBnn_uBeVFh2esLOcbwBUuW8fspMiaxSgOGWf3tJE_RTiyKPn04bCQCnEHDIPI-_tNA3E7bjibvbeDpEyd3u-i8N-S8lm4v3GFjCRPZo8Yg8Klenx7XrOri_ffb34UF19fv_x4s1V1Svd1pV2DtApVwvhle9AdRoJLPYl3IjDYUXoSWtCLWRLnhw64UHhykss9Z6z14vvbnZbWvU0TskOZpfC1qa9iTaYPyNj2Jh1_G4apcrAYvDi1iDFbzPlyWxD7mkY7Ehxzka05YdRSNX-H9UCsOQtoaDP_0Jv4pzG8hOmbqCRHcLRUCxUn2LOifxd3gLMobFmaawphZpDY01dNE9_L_hO8bOTBagXIJfQuKb06-l_uT5bRN5GY9cpZHP9pQaBUKYEjfIHzMm26g</recordid><startdate>201509</startdate><enddate>201509</enddate><creator>Kundave, V. R.</creator><creator>Patel, A. K.</creator><creator>Patel, P. V.</creator><creator>Hasnani, J. J.</creator><creator>Joshi, C. G.</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>201509</creationdate><title>Detection of theileriosis in cattle and buffaloes by polymerase chain reaction</title><author>Kundave, V. R. ; Patel, A. K. ; Patel, P. V. ; Hasnani, J. J. ; Joshi, C. G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5782-7bb06b5b211f5f905976e0a6cc5741976ede6fe77e67138efeb6b1f056df36013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>blood</topic><topic>buffaloes</topic><topic>Cattle</topic><topic>Diagnosis</topic><topic>Economic importance</topic><topic>genes</topic><topic>Health Promotion and Disease Prevention</topic><topic>Infectious Diseases</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Original</topic><topic>Original Article</topic><topic>Polymerase chain reaction</topic><topic>screening</topic><topic>staining</topic><topic>subtropics</topic><topic>surface antigens</topic><topic>Theileria annulata</topic><topic>Theileriosis</topic><topic>Tick-borne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kundave, V. R.</creatorcontrib><creatorcontrib>Patel, A. K.</creatorcontrib><creatorcontrib>Patel, P. V.</creatorcontrib><creatorcontrib>Hasnani, J. J.</creatorcontrib><creatorcontrib>Joshi, C. G.</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of parasitic diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kundave, V. R.</au><au>Patel, A. K.</au><au>Patel, P. V.</au><au>Hasnani, J. J.</au><au>Joshi, C. G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of theileriosis in cattle and buffaloes by polymerase chain reaction</atitle><jtitle>Journal of parasitic diseases</jtitle><stitle>J Parasit Dis</stitle><addtitle>J Parasit Dis</addtitle><date>2015-09</date><risdate>2015</risdate><volume>39</volume><issue>3</issue><spage>508</spage><epage>513</epage><pages>508-513</pages><issn>0971-7196</issn><eissn>0975-0703</eissn><abstract>Bovine tropical theileriosis caused by Theileria annulata is a tick-borne disease of great economic importance in tropical and subtropical regions of the world. The present study was undertaken to detect theilerosis in cattle and buffaloes by polymerase chain reaction (PCR). The diagnosis of theileriosis is usually carried out by blood smear staining technique, which is not sufficiently sensitive to detect the piroplasms in the carrier animals. In this study, a total of 116 samples were collected from infected as well as apparently healthy cattle and buffaloes. Screening of blood smears by Giemsa staining detected 15 samples (12.93 %) positive for Theileria piroplasms out of 116 samples. However, the PCR based screening using the specific primers from the major merozoite-piroplasm surface antigen sequence of T. annulata (Tams1) gene detected 74 samples (63.79 %) positive for T. annulata which included 59 samples found negative by Giemsa staining. Our study suggests that the PCR based screening is more sensitive and accurate method for diagnosis of tropical theileriosis in cattle and buffaloes.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>26345061</pmid><doi>10.1007/s12639-013-0386-2</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0971-7196 |
| ispartof | Journal of parasitic diseases, 2015-09, Vol.39 (3), p.508-513 |
| issn | 0971-7196 0975-0703 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4554556 |
| source | SpringerLink Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | blood buffaloes Cattle Diagnosis Economic importance genes Health Promotion and Disease Prevention Infectious Diseases Medicine Medicine & Public Health Original Original Article Polymerase chain reaction screening staining subtropics surface antigens Theileria annulata Theileriosis Tick-borne diseases |
| title | Detection of theileriosis in cattle and buffaloes by polymerase chain reaction |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T02%3A03%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20theileriosis%20in%20cattle%20and%20buffaloes%20by%20polymerase%20chain%20reaction&rft.jtitle=Journal%20of%20parasitic%20diseases&rft.au=Kundave,%20V.%20R.&rft.date=2015-09&rft.volume=39&rft.issue=3&rft.spage=508&rft.epage=513&rft.pages=508-513&rft.issn=0971-7196&rft.eissn=0975-0703&rft_id=info:doi/10.1007/s12639-013-0386-2&rft_dat=%3Cproquest_pubme%3E1710657430%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2404396058&rft_id=info:pmid/26345061&rfr_iscdi=true |