Enhanced secretion of a methyl parathion hydrolase in Pichia pastoris using a combinational strategy

Although Pichia pastoris has been successfully used to produce various recombinant heterologous proteins, the efficiency varies. In this study, we used methyl parathion hydrolase (MPH) from Ochrobactrum sp. M231 as an example to study the effect of protein amino acid sequence on secretion from P. pa...

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Veröffentlicht in:	Microbial cell factories 2015-08, Vol.14 (1), p.123-123, Article 123
Hauptverfasser:	Wang, Ping, Huang, Lu, Jiang, Hu, Tian, Jian, Chu, Xiaoyu, Wu, Ningfeng
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Analysis Enzymes Gene Dosage Genetic Engineering Health aspects Hydrolases Organisms, Genetically Modified Phosphoric Monoester Hydrolases - metabolism Physiological aspects Pichia - genetics Pichia - metabolism Protein C Protein Sorting Signals Recombinant Proteins - genetics Recombinant Proteins - metabolism RNA, Messenger - metabolism Sequence Analysis, Protein
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