Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark of Curatella americana L. (Dilleniaceae) against Candida Species
The ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian cerrado, in which crude extract showed antifungal activity in a preliminary study. In this work, the antifungal and cytotoxic properties of the crude extract, fractions, and isolated compounds from C. a...
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| description | The ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian cerrado, in which crude extract showed antifungal activity in a preliminary study. In this work, the antifungal and cytotoxic properties of the crude extract, fractions, and isolated compounds from C. americana were evaluated against the standard yeast strains Candida albicans, C. tropicalis, and C. parapsilosis, clinical isolates, and fluconazole-resistant strains. The combinatory effects between subfractions and isolated compounds and effects on cell morphology, virulence factors, and exogenous ergosterol were also evaluated. The MIC obtained against the Candida species including fluconazole-resistant strain ranged from 15.3 to 31.3 µg/mL for crude extract, 3.9 to 15.6 µg/mL for ethyl acetate fraction, and 7.8 to 31.3 µg/mL for subfractions. The isolated compounds identified as 4′-O-methyl-catechin, epicatechin-3-O-gallate, and 4′-O-methyl-catechin-3-O-gallate showed lower antifungal activity than the crude extract and fractions (MIC ranging from 31.3 to 125.0 µg/mL). The addition of exogenous ergosterol to yeast culture did not interfere in the antifungal activity of the extract and its fractions. Synergistic antifungal activity was observed between subfractions and isolated compounds. The effects on virulence factors and the different mechanisms of action compared to fluconazole and nystatin suggest that this ethnomedicinal plant may be an effective alternative treatment for candidiasis. |
| doi_str_mv | 10.1155/2015/673962 |
| format | Article |
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(Dilleniaceae) against Candida Species</title><source>PubMed (Medline)</source><source>Wiley Online Library</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><source>PubMed Central Open Access</source><creator>Nakamura, Celso Vataru ; Dias Filho, Benedito Prado ; Palazzo de Mello, João Carlos ; Santos, Patrícia Regina ; Mendes de Toledo, Cleyton Eduardo ; Ueda-Nakamura, Tânia</creator><contributor>van Vuuren, Sandy</contributor><creatorcontrib>Nakamura, Celso Vataru ; Dias Filho, Benedito Prado ; Palazzo de Mello, João Carlos ; Santos, Patrícia Regina ; Mendes de Toledo, Cleyton Eduardo ; Ueda-Nakamura, Tânia ; van Vuuren, Sandy</creatorcontrib><description>The ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian cerrado, in which crude extract showed antifungal activity in a preliminary study. In this work, the antifungal and cytotoxic properties of the crude extract, fractions, and isolated compounds from C. americana were evaluated against the standard yeast strains Candida albicans, C. tropicalis, and C. parapsilosis, clinical isolates, and fluconazole-resistant strains. The combinatory effects between subfractions and isolated compounds and effects on cell morphology, virulence factors, and exogenous ergosterol were also evaluated. The MIC obtained against the Candida species including fluconazole-resistant strain ranged from 15.3 to 31.3 µg/mL for crude extract, 3.9 to 15.6 µg/mL for ethyl acetate fraction, and 7.8 to 31.3 µg/mL for subfractions. The isolated compounds identified as 4′-O-methyl-catechin, epicatechin-3-O-gallate, and 4′-O-methyl-catechin-3-O-gallate showed lower antifungal activity than the crude extract and fractions (MIC ranging from 31.3 to 125.0 µg/mL). The addition of exogenous ergosterol to yeast culture did not interfere in the antifungal activity of the extract and its fractions. Synergistic antifungal activity was observed between subfractions and isolated compounds. The effects on virulence factors and the different mechanisms of action compared to fluconazole and nystatin suggest that this ethnomedicinal plant may be an effective alternative treatment for candidiasis.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2015/673962</identifier><identifier>PMID: 26347790</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Candida albicans ; Chromatography ; Curatella americana ; Dilleniaceae ; Drugs ; Flowers & plants ; Fractions ; Fungal infections ; Herbal medicine ; Laboratories ; Medical research ; Metabolism ; Studies</subject><ispartof>Evidence-based complementary and alternative medicine, 2015-01, Vol.2015 (2015), p.1-9</ispartof><rights>Copyright © 2015 Cleyton Eduardo Mendes de Toledo et al.</rights><rights>Copyright © 2015 Cleyton Eduardo Mendes de Toledo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright © 2015 Cleyton Eduardo Mendes de Toledo et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-70194f8c7fc4dea2b454e40409d8d71b3b3de243966ba0745108c1c3d85a5eeb3</citedby><cites>FETCH-LOGICAL-c463t-70194f8c7fc4dea2b454e40409d8d71b3b3de243966ba0745108c1c3d85a5eeb3</cites><orcidid>0000-0002-9911-7369</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548135/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4548135/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26347790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>van Vuuren, Sandy</contributor><creatorcontrib>Nakamura, Celso Vataru</creatorcontrib><creatorcontrib>Dias Filho, Benedito Prado</creatorcontrib><creatorcontrib>Palazzo de Mello, João Carlos</creatorcontrib><creatorcontrib>Santos, Patrícia Regina</creatorcontrib><creatorcontrib>Mendes de Toledo, Cleyton Eduardo</creatorcontrib><creatorcontrib>Ueda-Nakamura, Tânia</creatorcontrib><title>Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark of Curatella americana L. (Dilleniaceae) against Candida Species</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>The ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian cerrado, in which crude extract showed antifungal activity in a preliminary study. In this work, the antifungal and cytotoxic properties of the crude extract, fractions, and isolated compounds from C. americana were evaluated against the standard yeast strains Candida albicans, C. tropicalis, and C. parapsilosis, clinical isolates, and fluconazole-resistant strains. The combinatory effects between subfractions and isolated compounds and effects on cell morphology, virulence factors, and exogenous ergosterol were also evaluated. The MIC obtained against the Candida species including fluconazole-resistant strain ranged from 15.3 to 31.3 µg/mL for crude extract, 3.9 to 15.6 µg/mL for ethyl acetate fraction, and 7.8 to 31.3 µg/mL for subfractions. The isolated compounds identified as 4′-O-methyl-catechin, epicatechin-3-O-gallate, and 4′-O-methyl-catechin-3-O-gallate showed lower antifungal activity than the crude extract and fractions (MIC ranging from 31.3 to 125.0 µg/mL). The addition of exogenous ergosterol to yeast culture did not interfere in the antifungal activity of the extract and its fractions. Synergistic antifungal activity was observed between subfractions and isolated compounds. The effects on virulence factors and the different mechanisms of action compared to fluconazole and nystatin suggest that this ethnomedicinal plant may be an effective alternative treatment for candidiasis.</description><subject>Candida albicans</subject><subject>Chromatography</subject><subject>Curatella americana</subject><subject>Dilleniaceae</subject><subject>Drugs</subject><subject>Flowers & plants</subject><subject>Fractions</subject><subject>Fungal infections</subject><subject>Herbal medicine</subject><subject>Laboratories</subject><subject>Medical research</subject><subject>Metabolism</subject><subject>Studies</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkk1v1DAQhiMEoqVw4o4scSkf29qOHScXpLK0gLQSlQCJmzWxJ1uXxE7thI8fwv_Fy5ZV4QKnGcmP3_E7foviIaNHjEl5zCmTx5Uqm4rfKvaZEmwheF3f3vXq015xL6VLSnmjlLpb7PGqFEo1dL_4ceIn181-DT05j2HEODlMJHRkGWeL5PTbFMFM6Tk521QXfG7BW3I-R9c5tGQZhjHM3ibSxTCQlxA__7o-R5iw74HAgNEZ8EBWR-Twlet79A4MAj4hsAbn00SWWdJZIO9HNHn-_eJOB33CB9f1oPh4dvph-Waxevf67fJktTCiKqeFoqwRXW1UZ4RF4K2QAgUVtLG1Vawt29IiF3kzVQtUCclobZgpbS1BIrblQfFiqzvO7YDWoM9uez1GN0D8rgM4_eeJdxd6Hb7oPKhmpcwCh9cCMVzNmCY9uGQ2tj2GOWmmuKoaJjP8b5TRSoqKlxl9_Bd6Gebo8yYyRRuef1o2mXq2pUwMKUXsdu9mVG-SoTfJ0NtkZPrRTas79ncUMvB0C1w4b-Gr-z81zAh2cAPOeqIpfwLMo8r_</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Nakamura, Celso Vataru</creator><creator>Dias Filho, Benedito Prado</creator><creator>Palazzo de Mello, João Carlos</creator><creator>Santos, Patrícia Regina</creator><creator>Mendes de Toledo, Cleyton Eduardo</creator><creator>Ueda-Nakamura, Tânia</creator><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>M7N</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9911-7369</orcidid></search><sort><creationdate>20150101</creationdate><title>Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark of Curatella americana L. 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(Dilleniaceae) against Candida Species</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>2015</volume><issue>2015</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>The ethnomedicinal plant Curatella americana L. (Dilleniaceae) is a common shrub in the Brazilian cerrado, in which crude extract showed antifungal activity in a preliminary study. In this work, the antifungal and cytotoxic properties of the crude extract, fractions, and isolated compounds from C. americana were evaluated against the standard yeast strains Candida albicans, C. tropicalis, and C. parapsilosis, clinical isolates, and fluconazole-resistant strains. The combinatory effects between subfractions and isolated compounds and effects on cell morphology, virulence factors, and exogenous ergosterol were also evaluated. The MIC obtained against the Candida species including fluconazole-resistant strain ranged from 15.3 to 31.3 µg/mL for crude extract, 3.9 to 15.6 µg/mL for ethyl acetate fraction, and 7.8 to 31.3 µg/mL for subfractions. The isolated compounds identified as 4′-O-methyl-catechin, epicatechin-3-O-gallate, and 4′-O-methyl-catechin-3-O-gallate showed lower antifungal activity than the crude extract and fractions (MIC ranging from 31.3 to 125.0 µg/mL). The addition of exogenous ergosterol to yeast culture did not interfere in the antifungal activity of the extract and its fractions. Synergistic antifungal activity was observed between subfractions and isolated compounds. The effects on virulence factors and the different mechanisms of action compared to fluconazole and nystatin suggest that this ethnomedicinal plant may be an effective alternative treatment for candidiasis.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>26347790</pmid><doi>10.1155/2015/673962</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9911-7369</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Candida albicans Chromatography Curatella americana Dilleniaceae Drugs Flowers & plants Fractions Fungal infections Herbal medicine Laboratories Medical research Metabolism Studies |
| title | Antifungal Properties of Crude Extracts, Fractions, and Purified Compounds from Bark of Curatella americana L. (Dilleniaceae) against Candida Species |
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