High‐Resolution Multi‐Photon Imaging of Morphological Structures of Caenorhabditis elegans

In this protocol, we combine two‐photon excitation fluorescence with nonlinear optical measurements to reconstruct the three‐dimensional architecture of the pharyngeal region and the muscular system of the anterior and mid‐body region of Caenorhabditis elegans (C. elegans). Femto‐second laser pulses...

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Veröffentlicht in:	Current Protocols in Toxicology 2015-05, Vol.64 (1), p.11.19.1-11.19.11
Hauptverfasser:	Bixel, Gabriele M., Fretham, Stephanie J.B., Aschner, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals C. elegans Caenorhabditis elegans - anatomy & histology Caenorhabditis elegans - ultrastructure Imaging, Three-Dimensional - methods label‐free imaging Microscopy, Fluorescence, Multiphoton - methods multi‐photon microscopy Muscles - anatomy & histology Neurons - ultrastructure Nonlinear Dynamics Pharynx - anatomy & histology SHG THG
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