Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry

Cancer cells respond to matrix mechanical stiffness in a complex manner using a coordinated, hierarchical mechano-chemical system composed of adhesion receptors and associated signal transduction membrane proteins, the cytoskeletal architecture, and molecular motors1, 2. Mechanosensitivity of differ...

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Veröffentlicht in:	Journal of Visualized Experiments 2015-06 (100), p.e52532-e52532
Hauptverfasser:	Ali, M. Yakut, Anand, Sandeep V., Tangella, Krishnarao, Ramkumar, Davendra, Saif, Taher A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrylic Resins - chemistry Bioengineering Biomechanical Phenomena Cell Culture Techniques - methods Colon - cytology Colonic Neoplasms - pathology Colonic Neoplasms - surgery Elasticity Extracellular Matrix Proteins - chemistry Flow Cytometry - methods Humans Hydrogels - chemistry Microscopy, Fluorescence - methods
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