Optimized Agrobacterium-mediated sorghum transformation protocol and molecular data of transgenic sorghum plants

Agrobacterium-mediated sorghum transformation frequency has been enhanced significantly via medium optimization using immature embryos from sorghum variety TX430 as the target tissue. The new transformation protocol includes the addition of elevated copper sulfate and 6-benzylaminopurine in the rest...

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Veröffentlicht in:	In vitro cellular & developmental biology. Plant 2014, Vol.50 (1), p.9-18
Hauptverfasser:	Wu, Emily, Lenderts, Brian, Glassman, Kimberly, Berezowska-Kaniewska, Maya, Christensen, Heather, Asmus, Tracy, Zhen, Shifu, Chu, Uyen, Cho, Myeong-Je, Zhao, Zuo-Yu
Format:	Artikel
Sprache:	eng
Schlagworte:	Agrobacterium Biomedical and Life Sciences BIOTECHNOLOGY/GENETIC TRANSFORMATION/FUNCTIONAL GENOMICS Callus Cell Biology Climate change copper sulfate Corn Deoxyribonucleic acid Developmental Biology DNA Embryos Genes genetic markers Genetic vectors Grain Immatures Life Sciences Plant Breeding/Biotechnology Plant cells Plant Genetics and Genomics Plant Sciences Plants Product development quantitative polymerase chain reaction Sorghum Studies transfer DNA Transgenic plants
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