Platelet hyperactivity and fibrin clot structure in transient ischemic attack individuals in the presence of metabolic syndrome: a microscopy and thromboelastography study
Strokes are commonly preceded by transient ischemic attacks (TIAs). TIA is often associated with metabolic syndrome (causing chronic inflammation), resulting in a proinflammatory- and procoagulant-environment. The aim of this study was to determine whether platelet- and fibrin network-morphology or...
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| Veröffentlicht in: | Cardiovascular Diabetology 2015-07, Vol.14 (1), p.86-86, Article 86 |
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| creator | van Rooy, Mia-Jeanne Duim, Wiebren Ehlers, Rene Buys, Antoinette V Pretorius, Etheresia |
| description | Strokes are commonly preceded by transient ischemic attacks (TIAs). TIA is often associated with metabolic syndrome (causing chronic inflammation), resulting in a proinflammatory- and procoagulant-environment. The aim of this study was to determine whether platelet- and fibrin network-morphology or coagulation profiles of individuals that suffered a TIA in the presence of metabolic syndrome was altered when compared to healthy individuals.
The study consisted of 40 voluntary participants. Twenty individuals that suffered a TIA in the previous 48 h with at least two metabolic syndrome risk factors present and twenty healthy age-matched controls. Scanning electron- and atomic force microscopy was used to study platelet- and fibrin-morphology, atomic force microscopy was used to study platelet- and fibrin fiber-elasticity and thromboelastography for the study of coagulation profiles. Statistical analysis was performed to compare the two groups. In all cases a p-value of less than 0.05 was considered statistically significant.
Platelets of the control group appeared spherical with few pseudopodia present while the platelets of the TIA individuals presented with numerous pseudopodia and spreading, indicating activation. Platelet aggregation was also present. The fibrin networks of the healthy individuals consist of thick and thin fibers that form an organized network of fibers. The fibrin networks of the TIA individuals appeared less organized with less taut fibers. Fibrin fiber thickness was found to be significantly increased in the TIA group (p-value 0.05) from the healthy controls, indicating a normal functioning coagulation cascade.
The findings indicate that pathological clot formation is not caused by alterations in the coagulation cascade but rather by the premature activation of platelets (as a result of chronic inflammation) that in turn causes altered fibrin formation. |
| doi_str_mv | 10.1186/s12933-015-0249-5 |
| format | Article |
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The study consisted of 40 voluntary participants. Twenty individuals that suffered a TIA in the previous 48 h with at least two metabolic syndrome risk factors present and twenty healthy age-matched controls. Scanning electron- and atomic force microscopy was used to study platelet- and fibrin-morphology, atomic force microscopy was used to study platelet- and fibrin fiber-elasticity and thromboelastography for the study of coagulation profiles. Statistical analysis was performed to compare the two groups. In all cases a p-value of less than 0.05 was considered statistically significant.
Platelets of the control group appeared spherical with few pseudopodia present while the platelets of the TIA individuals presented with numerous pseudopodia and spreading, indicating activation. Platelet aggregation was also present. The fibrin networks of the healthy individuals consist of thick and thin fibers that form an organized network of fibers. The fibrin networks of the TIA individuals appeared less organized with less taut fibers. Fibrin fiber thickness was found to be significantly increased in the TIA group (p-value <0.001) when compared to healthy controls. The thicker fibers formed irregular networks with thick masses of fibrin fibers. Platelet and fibrin fiber elasticity was found to be significantly lower in the experimental group (p-value 0.0042 and p-value 0.0007 respectively). The hemostatic profiles of the diseased individuals did not differ significantly (p-value > 0.05) from the healthy controls, indicating a normal functioning coagulation cascade.
The findings indicate that pathological clot formation is not caused by alterations in the coagulation cascade but rather by the premature activation of platelets (as a result of chronic inflammation) that in turn causes altered fibrin formation.</description><identifier>ISSN: 1475-2840</identifier><identifier>EISSN: 1475-2840</identifier><identifier>DOI: 10.1186/s12933-015-0249-5</identifier><identifier>PMID: 26140921</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Adult ; Aged ; Atomic force microscopy ; Blood Coagulation ; Blood Platelets - metabolism ; Blood Platelets - ultrastructure ; Care and treatment ; Case-Control Studies ; Cerebral ischemia ; Comparative analysis ; Complications and side effects ; Elasticity ; Female ; Fibrin - metabolism ; Fibrin - ultrastructure ; Fibrinolysis ; Humans ; Ischemic Attack, Transient - blood ; Ischemic Attack, Transient - complications ; Ischemic Attack, Transient - diagnosis ; Male ; Metabolic Syndrome - blood ; Metabolic Syndrome - complications ; Metabolic Syndrome - diagnosis ; Microscopy, Atomic Force ; Microscopy, Electron, Scanning ; Middle Aged ; Original Investigation ; Platelet Activation ; Platelet Aggregation ; Predictive Value of Tests ; Risk Factors ; Thrombelastography</subject><ispartof>Cardiovascular Diabetology, 2015-07, Vol.14 (1), p.86-86, Article 86</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>van Rooy et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-35f823afbd3cc9f240c5b5d16f60eecb48deca261b3befca4e974c62151c4ac73</citedby><cites>FETCH-LOGICAL-c532t-35f823afbd3cc9f240c5b5d16f60eecb48deca261b3befca4e974c62151c4ac73</cites><orcidid>0000-0002-9108-2384</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542104/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542104/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26140921$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van Rooy, Mia-Jeanne</creatorcontrib><creatorcontrib>Duim, Wiebren</creatorcontrib><creatorcontrib>Ehlers, Rene</creatorcontrib><creatorcontrib>Buys, Antoinette V</creatorcontrib><creatorcontrib>Pretorius, Etheresia</creatorcontrib><title>Platelet hyperactivity and fibrin clot structure in transient ischemic attack individuals in the presence of metabolic syndrome: a microscopy and thromboelastography study</title><title>Cardiovascular Diabetology</title><addtitle>Cardiovasc Diabetol</addtitle><description>Strokes are commonly preceded by transient ischemic attacks (TIAs). TIA is often associated with metabolic syndrome (causing chronic inflammation), resulting in a proinflammatory- and procoagulant-environment. The aim of this study was to determine whether platelet- and fibrin network-morphology or coagulation profiles of individuals that suffered a TIA in the presence of metabolic syndrome was altered when compared to healthy individuals.
The study consisted of 40 voluntary participants. Twenty individuals that suffered a TIA in the previous 48 h with at least two metabolic syndrome risk factors present and twenty healthy age-matched controls. Scanning electron- and atomic force microscopy was used to study platelet- and fibrin-morphology, atomic force microscopy was used to study platelet- and fibrin fiber-elasticity and thromboelastography for the study of coagulation profiles. Statistical analysis was performed to compare the two groups. In all cases a p-value of less than 0.05 was considered statistically significant.
Platelets of the control group appeared spherical with few pseudopodia present while the platelets of the TIA individuals presented with numerous pseudopodia and spreading, indicating activation. Platelet aggregation was also present. The fibrin networks of the healthy individuals consist of thick and thin fibers that form an organized network of fibers. The fibrin networks of the TIA individuals appeared less organized with less taut fibers. Fibrin fiber thickness was found to be significantly increased in the TIA group (p-value <0.001) when compared to healthy controls. The thicker fibers formed irregular networks with thick masses of fibrin fibers. Platelet and fibrin fiber elasticity was found to be significantly lower in the experimental group (p-value 0.0042 and p-value 0.0007 respectively). The hemostatic profiles of the diseased individuals did not differ significantly (p-value > 0.05) from the healthy controls, indicating a normal functioning coagulation cascade.
The findings indicate that pathological clot formation is not caused by alterations in the coagulation cascade but rather by the premature activation of platelets (as a result of chronic inflammation) that in turn causes altered fibrin formation.</description><subject>Adult</subject><subject>Aged</subject><subject>Atomic force microscopy</subject><subject>Blood Coagulation</subject><subject>Blood Platelets - metabolism</subject><subject>Blood Platelets - ultrastructure</subject><subject>Care and treatment</subject><subject>Case-Control Studies</subject><subject>Cerebral ischemia</subject><subject>Comparative analysis</subject><subject>Complications and side effects</subject><subject>Elasticity</subject><subject>Female</subject><subject>Fibrin - metabolism</subject><subject>Fibrin - ultrastructure</subject><subject>Fibrinolysis</subject><subject>Humans</subject><subject>Ischemic Attack, Transient - blood</subject><subject>Ischemic Attack, Transient - complications</subject><subject>Ischemic Attack, Transient - diagnosis</subject><subject>Male</subject><subject>Metabolic Syndrome - blood</subject><subject>Metabolic Syndrome - complications</subject><subject>Metabolic Syndrome - diagnosis</subject><subject>Microscopy, Atomic Force</subject><subject>Microscopy, Electron, Scanning</subject><subject>Middle Aged</subject><subject>Original Investigation</subject><subject>Platelet Activation</subject><subject>Platelet Aggregation</subject><subject>Predictive Value of Tests</subject><subject>Risk Factors</subject><subject>Thrombelastography</subject><issn>1475-2840</issn><issn>1475-2840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUstu1TAUjBCIlsIHsEFesknxM7lhgVRVvKRKsIC15djHNwbHDrZTKd_ET-JLSlXkha1zZubY42malwRfEnLo3mRCB8ZaTESLKR9a8ag5J7wXLT1w_PjB-ax5lvMPjEl_6MjT5ox2hOOBkvPm91evCngoaNoWSEoXd-vKhlQwyLoxuYC0jwXlklZd1gSoVkpSITsIBbmsJ5idRqoUpX_Wpql8syqf_wInQEuCDEEDihbNUNQYfcXnLZgUZ3iLFKr8FLOOyz62TLUxRvAql3hMapm2On412_Pmia3C8OJuv2i-f3j_7fpTe_Pl4-frq5tWC0ZLy4Q9UKbsaJjWg6UcazEKQzrbYQA98oMBraoFIxvBasVh6LnuKBFEc6V7dtG823WXdZzB6PrQpLxckptV2mRUTv7fCW6Sx3grueCUYF4FXt8JpPhrhVzkXI0C71WAuGZJesx6jAUfKvRyhx6VB-mCjVVR12VOtsYA1tX6leD1LzEnXSWQnXDyLCew9_ciWJ5SIfdUyJoKeUqFFJXz6uGD7hn_YsD-ALwjumI</recordid><startdate>20150704</startdate><enddate>20150704</enddate><creator>van Rooy, Mia-Jeanne</creator><creator>Duim, Wiebren</creator><creator>Ehlers, Rene</creator><creator>Buys, Antoinette V</creator><creator>Pretorius, Etheresia</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9108-2384</orcidid></search><sort><creationdate>20150704</creationdate><title>Platelet hyperactivity and fibrin clot structure in transient ischemic attack individuals in the presence of metabolic syndrome: a microscopy and thromboelastography study</title><author>van Rooy, Mia-Jeanne ; Duim, Wiebren ; Ehlers, Rene ; Buys, Antoinette V ; Pretorius, Etheresia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-35f823afbd3cc9f240c5b5d16f60eecb48deca261b3befca4e974c62151c4ac73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Atomic force microscopy</topic><topic>Blood Coagulation</topic><topic>Blood Platelets - metabolism</topic><topic>Blood Platelets - ultrastructure</topic><topic>Care and treatment</topic><topic>Case-Control Studies</topic><topic>Cerebral ischemia</topic><topic>Comparative analysis</topic><topic>Complications and side effects</topic><topic>Elasticity</topic><topic>Female</topic><topic>Fibrin - metabolism</topic><topic>Fibrin - ultrastructure</topic><topic>Fibrinolysis</topic><topic>Humans</topic><topic>Ischemic Attack, Transient - blood</topic><topic>Ischemic Attack, Transient - complications</topic><topic>Ischemic Attack, Transient - diagnosis</topic><topic>Male</topic><topic>Metabolic Syndrome - blood</topic><topic>Metabolic Syndrome - complications</topic><topic>Metabolic Syndrome - diagnosis</topic><topic>Microscopy, Atomic Force</topic><topic>Microscopy, Electron, Scanning</topic><topic>Middle Aged</topic><topic>Original Investigation</topic><topic>Platelet Activation</topic><topic>Platelet Aggregation</topic><topic>Predictive Value of Tests</topic><topic>Risk Factors</topic><topic>Thrombelastography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van Rooy, Mia-Jeanne</creatorcontrib><creatorcontrib>Duim, Wiebren</creatorcontrib><creatorcontrib>Ehlers, Rene</creatorcontrib><creatorcontrib>Buys, Antoinette V</creatorcontrib><creatorcontrib>Pretorius, Etheresia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale Academic OneFile</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cardiovascular Diabetology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Rooy, Mia-Jeanne</au><au>Duim, Wiebren</au><au>Ehlers, Rene</au><au>Buys, Antoinette V</au><au>Pretorius, Etheresia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Platelet hyperactivity and fibrin clot structure in transient ischemic attack individuals in the presence of metabolic syndrome: a microscopy and thromboelastography study</atitle><jtitle>Cardiovascular Diabetology</jtitle><addtitle>Cardiovasc Diabetol</addtitle><date>2015-07-04</date><risdate>2015</risdate><volume>14</volume><issue>1</issue><spage>86</spage><epage>86</epage><pages>86-86</pages><artnum>86</artnum><issn>1475-2840</issn><eissn>1475-2840</eissn><abstract>Strokes are commonly preceded by transient ischemic attacks (TIAs). TIA is often associated with metabolic syndrome (causing chronic inflammation), resulting in a proinflammatory- and procoagulant-environment. The aim of this study was to determine whether platelet- and fibrin network-morphology or coagulation profiles of individuals that suffered a TIA in the presence of metabolic syndrome was altered when compared to healthy individuals.
The study consisted of 40 voluntary participants. Twenty individuals that suffered a TIA in the previous 48 h with at least two metabolic syndrome risk factors present and twenty healthy age-matched controls. Scanning electron- and atomic force microscopy was used to study platelet- and fibrin-morphology, atomic force microscopy was used to study platelet- and fibrin fiber-elasticity and thromboelastography for the study of coagulation profiles. Statistical analysis was performed to compare the two groups. In all cases a p-value of less than 0.05 was considered statistically significant.
Platelets of the control group appeared spherical with few pseudopodia present while the platelets of the TIA individuals presented with numerous pseudopodia and spreading, indicating activation. Platelet aggregation was also present. The fibrin networks of the healthy individuals consist of thick and thin fibers that form an organized network of fibers. The fibrin networks of the TIA individuals appeared less organized with less taut fibers. Fibrin fiber thickness was found to be significantly increased in the TIA group (p-value <0.001) when compared to healthy controls. The thicker fibers formed irregular networks with thick masses of fibrin fibers. Platelet and fibrin fiber elasticity was found to be significantly lower in the experimental group (p-value 0.0042 and p-value 0.0007 respectively). The hemostatic profiles of the diseased individuals did not differ significantly (p-value > 0.05) from the healthy controls, indicating a normal functioning coagulation cascade.
The findings indicate that pathological clot formation is not caused by alterations in the coagulation cascade but rather by the premature activation of platelets (as a result of chronic inflammation) that in turn causes altered fibrin formation.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26140921</pmid><doi>10.1186/s12933-015-0249-5</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-9108-2384</orcidid><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Springer Nature OA Free Journals; Springer Nature - Complete Springer Journals; PubMed Central |
| subjects | Adult Aged Atomic force microscopy Blood Coagulation Blood Platelets - metabolism Blood Platelets - ultrastructure Care and treatment Case-Control Studies Cerebral ischemia Comparative analysis Complications and side effects Elasticity Female Fibrin - metabolism Fibrin - ultrastructure Fibrinolysis Humans Ischemic Attack, Transient - blood Ischemic Attack, Transient - complications Ischemic Attack, Transient - diagnosis Male Metabolic Syndrome - blood Metabolic Syndrome - complications Metabolic Syndrome - diagnosis Microscopy, Atomic Force Microscopy, Electron, Scanning Middle Aged Original Investigation Platelet Activation Platelet Aggregation Predictive Value of Tests Risk Factors Thrombelastography |
| title | Platelet hyperactivity and fibrin clot structure in transient ischemic attack individuals in the presence of metabolic syndrome: a microscopy and thromboelastography study |
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