Wnt Signaling Regulates Pulp Volume and Dentin Thickness
ABSTRACT Odontoblasts, cementoblasts, ameloblasts, and osteoblasts all form mineralized tissues in the craniofacial complex, and all these cell types exhibit active Wnt signaling during postnatal life. We set out to understand the functions of this Wnt signaling, by evaluating the phenotypes of mice...
Gespeichert in:
| Veröffentlicht in: | Journal of bone and mineral research 2014-04, Vol.29 (4), p.892-901 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 901 |
|---|---|
| container_issue | 4 |
| container_start_page | 892 |
| container_title | Journal of bone and mineral research |
| container_volume | 29 |
| creator | Lim, Won Hee Liu, Bo Cheng, Du Hunter, Daniel J Zhong, Zhendong Ramos, Daniel M Williams, Bart O Sharpe, Paul T Bardet, Claire Mah, Su‐jung Helms, Jill A |
| description | ABSTRACT
Odontoblasts, cementoblasts, ameloblasts, and osteoblasts all form mineralized tissues in the craniofacial complex, and all these cell types exhibit active Wnt signaling during postnatal life. We set out to understand the functions of this Wnt signaling, by evaluating the phenotypes of mice in which the essential Wnt chaperone protein, Wntless was eliminated. The deletion of Wls was restricted to cells expressing Osteocalcin (OCN), which in addition to osteoblasts includes odontoblasts, cementoblasts, and ameloblasts. Dentin, cementum, enamel, and bone all formed in OCN‐Cre;Wlsfl/fl mice but their homeostasis was dramatically affected. The most notable feature was a significant increase in dentin volume and density. We attribute this gain in dentin volume to a Wnt‐mediated misregulation of Runx2. Normally, Wnt signaling stimulates Runx2, which in turn inhibits dentin sialoprotein (DSP); this inhibition must be relieved for odontoblasts to differentiate. In OCN‐Cre;Wlsfl/fl mice, Wnt pathway activation is reduced and Runx2 levels decline. The Runx2‐mediated repression of DSP is relieved and odontoblast differentiation is accordingly enhanced. This study demonstrates the importance of Wnt signaling in the homeostasis of mineralized tissues of the craniofacial complex. © 2014 American Society for Bone and Mineral Research. |
| doi_str_mv | 10.1002/jbmr.2088 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4541795</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3249939131</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5098-77ca7b09a46280844f0101577d47d2797055bf903b13ac172854a65d84b781e33</originalsourceid><addsrcrecordid>eNp1kU1PGzEQhi0EKiHtgT-AVuIChw1jr722L0iQUmiVqhUNcLS8u05w8HrTdZYq_x7no6hU4jTSzKNHr-ZF6BDDAAOQs1lRtwMCQuygHmYkS2ku8C7qxQ1NgWZ4Hx2EMAOAnOX5B7RPMinzTOY9JB78Ivllp14766fJrZl2Ti9MSH52bp7cN66rTaJ9lXw2fmF9Mn605ZM3IXxEexPtgvm0nX109-VqPLxJRz-uvw4vRmnJQIqU81LzAqSmOREgKJ0ABsw4ryivCJccGCsmErICZ7rEnAhGdc4qQQsusMmyPjrfeOddUZuqjDFa7dS8tbVul6rRVr29ePuops2zooxiLlkUnGwFbfO7M2GhahtK45z2pumCwjEnBUk4iejxf-is6dr4mjUlqMQ0vq2PTjdU2TYhtGbyGgaDWvWhVn2oVR-RPfo3_Sv5t4AInG2AP9aZ5fsm9e3y--1a-QKoqZL6</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1508491496</pqid></control><display><type>article</type><title>Wnt Signaling Regulates Pulp Volume and Dentin Thickness</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Lim, Won Hee ; Liu, Bo ; Cheng, Du ; Hunter, Daniel J ; Zhong, Zhendong ; Ramos, Daniel M ; Williams, Bart O ; Sharpe, Paul T ; Bardet, Claire ; Mah, Su‐jung ; Helms, Jill A</creator><creatorcontrib>Lim, Won Hee ; Liu, Bo ; Cheng, Du ; Hunter, Daniel J ; Zhong, Zhendong ; Ramos, Daniel M ; Williams, Bart O ; Sharpe, Paul T ; Bardet, Claire ; Mah, Su‐jung ; Helms, Jill A</creatorcontrib><description>ABSTRACT
Odontoblasts, cementoblasts, ameloblasts, and osteoblasts all form mineralized tissues in the craniofacial complex, and all these cell types exhibit active Wnt signaling during postnatal life. We set out to understand the functions of this Wnt signaling, by evaluating the phenotypes of mice in which the essential Wnt chaperone protein, Wntless was eliminated. The deletion of Wls was restricted to cells expressing Osteocalcin (OCN), which in addition to osteoblasts includes odontoblasts, cementoblasts, and ameloblasts. Dentin, cementum, enamel, and bone all formed in OCN‐Cre;Wlsfl/fl mice but their homeostasis was dramatically affected. The most notable feature was a significant increase in dentin volume and density. We attribute this gain in dentin volume to a Wnt‐mediated misregulation of Runx2. Normally, Wnt signaling stimulates Runx2, which in turn inhibits dentin sialoprotein (DSP); this inhibition must be relieved for odontoblasts to differentiate. In OCN‐Cre;Wlsfl/fl mice, Wnt pathway activation is reduced and Runx2 levels decline. The Runx2‐mediated repression of DSP is relieved and odontoblast differentiation is accordingly enhanced. This study demonstrates the importance of Wnt signaling in the homeostasis of mineralized tissues of the craniofacial complex. © 2014 American Society for Bone and Mineral Research.</description><identifier>ISSN: 0884-0431</identifier><identifier>EISSN: 1523-4681</identifier><identifier>DOI: 10.1002/jbmr.2088</identifier><identifier>PMID: 23996396</identifier><identifier>CODEN: JBMREJ</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Animals ; Core Binding Factor Alpha 1 Subunit - genetics ; Dental Pulp - anatomy & histology ; DENTIN ; Dentin - anatomy & histology ; Mice ; ODONTOBLAST ; PULP ; Real-Time Polymerase Chain Reaction ; Signal Transduction ; Wnt Proteins - metabolism ; WNT SIGNALING PATHWAY</subject><ispartof>Journal of bone and mineral research, 2014-04, Vol.29 (4), p.892-901</ispartof><rights>2014 American Society for Bone and Mineral Research</rights><rights>2014 American Society for Bone and Mineral Research.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5098-77ca7b09a46280844f0101577d47d2797055bf903b13ac172854a65d84b781e33</citedby><cites>FETCH-LOGICAL-c5098-77ca7b09a46280844f0101577d47d2797055bf903b13ac172854a65d84b781e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbmr.2088$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbmr.2088$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23996396$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lim, Won Hee</creatorcontrib><creatorcontrib>Liu, Bo</creatorcontrib><creatorcontrib>Cheng, Du</creatorcontrib><creatorcontrib>Hunter, Daniel J</creatorcontrib><creatorcontrib>Zhong, Zhendong</creatorcontrib><creatorcontrib>Ramos, Daniel M</creatorcontrib><creatorcontrib>Williams, Bart O</creatorcontrib><creatorcontrib>Sharpe, Paul T</creatorcontrib><creatorcontrib>Bardet, Claire</creatorcontrib><creatorcontrib>Mah, Su‐jung</creatorcontrib><creatorcontrib>Helms, Jill A</creatorcontrib><title>Wnt Signaling Regulates Pulp Volume and Dentin Thickness</title><title>Journal of bone and mineral research</title><addtitle>J Bone Miner Res</addtitle><description>ABSTRACT
Odontoblasts, cementoblasts, ameloblasts, and osteoblasts all form mineralized tissues in the craniofacial complex, and all these cell types exhibit active Wnt signaling during postnatal life. We set out to understand the functions of this Wnt signaling, by evaluating the phenotypes of mice in which the essential Wnt chaperone protein, Wntless was eliminated. The deletion of Wls was restricted to cells expressing Osteocalcin (OCN), which in addition to osteoblasts includes odontoblasts, cementoblasts, and ameloblasts. Dentin, cementum, enamel, and bone all formed in OCN‐Cre;Wlsfl/fl mice but their homeostasis was dramatically affected. The most notable feature was a significant increase in dentin volume and density. We attribute this gain in dentin volume to a Wnt‐mediated misregulation of Runx2. Normally, Wnt signaling stimulates Runx2, which in turn inhibits dentin sialoprotein (DSP); this inhibition must be relieved for odontoblasts to differentiate. In OCN‐Cre;Wlsfl/fl mice, Wnt pathway activation is reduced and Runx2 levels decline. The Runx2‐mediated repression of DSP is relieved and odontoblast differentiation is accordingly enhanced. This study demonstrates the importance of Wnt signaling in the homeostasis of mineralized tissues of the craniofacial complex. © 2014 American Society for Bone and Mineral Research.</description><subject>Animals</subject><subject>Core Binding Factor Alpha 1 Subunit - genetics</subject><subject>Dental Pulp - anatomy & histology</subject><subject>DENTIN</subject><subject>Dentin - anatomy & histology</subject><subject>Mice</subject><subject>ODONTOBLAST</subject><subject>PULP</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Signal Transduction</subject><subject>Wnt Proteins - metabolism</subject><subject>WNT SIGNALING PATHWAY</subject><issn>0884-0431</issn><issn>1523-4681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1PGzEQhi0EKiHtgT-AVuIChw1jr722L0iQUmiVqhUNcLS8u05w8HrTdZYq_x7no6hU4jTSzKNHr-ZF6BDDAAOQs1lRtwMCQuygHmYkS2ku8C7qxQ1NgWZ4Hx2EMAOAnOX5B7RPMinzTOY9JB78Ivllp14766fJrZl2Ti9MSH52bp7cN66rTaJ9lXw2fmF9Mn605ZM3IXxEexPtgvm0nX109-VqPLxJRz-uvw4vRmnJQIqU81LzAqSmOREgKJ0ABsw4ryivCJccGCsmErICZ7rEnAhGdc4qQQsusMmyPjrfeOddUZuqjDFa7dS8tbVul6rRVr29ePuops2zooxiLlkUnGwFbfO7M2GhahtK45z2pumCwjEnBUk4iejxf-is6dr4mjUlqMQ0vq2PTjdU2TYhtGbyGgaDWvWhVn2oVR-RPfo3_Sv5t4AInG2AP9aZ5fsm9e3y--1a-QKoqZL6</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Lim, Won Hee</creator><creator>Liu, Bo</creator><creator>Cheng, Du</creator><creator>Hunter, Daniel J</creator><creator>Zhong, Zhendong</creator><creator>Ramos, Daniel M</creator><creator>Williams, Bart O</creator><creator>Sharpe, Paul T</creator><creator>Bardet, Claire</creator><creator>Mah, Su‐jung</creator><creator>Helms, Jill A</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TS</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201404</creationdate><title>Wnt Signaling Regulates Pulp Volume and Dentin Thickness</title><author>Lim, Won Hee ; Liu, Bo ; Cheng, Du ; Hunter, Daniel J ; Zhong, Zhendong ; Ramos, Daniel M ; Williams, Bart O ; Sharpe, Paul T ; Bardet, Claire ; Mah, Su‐jung ; Helms, Jill A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5098-77ca7b09a46280844f0101577d47d2797055bf903b13ac172854a65d84b781e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Core Binding Factor Alpha 1 Subunit - genetics</topic><topic>Dental Pulp - anatomy & histology</topic><topic>DENTIN</topic><topic>Dentin - anatomy & histology</topic><topic>Mice</topic><topic>ODONTOBLAST</topic><topic>PULP</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Signal Transduction</topic><topic>Wnt Proteins - metabolism</topic><topic>WNT SIGNALING PATHWAY</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lim, Won Hee</creatorcontrib><creatorcontrib>Liu, Bo</creatorcontrib><creatorcontrib>Cheng, Du</creatorcontrib><creatorcontrib>Hunter, Daniel J</creatorcontrib><creatorcontrib>Zhong, Zhendong</creatorcontrib><creatorcontrib>Ramos, Daniel M</creatorcontrib><creatorcontrib>Williams, Bart O</creatorcontrib><creatorcontrib>Sharpe, Paul T</creatorcontrib><creatorcontrib>Bardet, Claire</creatorcontrib><creatorcontrib>Mah, Su‐jung</creatorcontrib><creatorcontrib>Helms, Jill A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Physical Education Index</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of bone and mineral research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lim, Won Hee</au><au>Liu, Bo</au><au>Cheng, Du</au><au>Hunter, Daniel J</au><au>Zhong, Zhendong</au><au>Ramos, Daniel M</au><au>Williams, Bart O</au><au>Sharpe, Paul T</au><au>Bardet, Claire</au><au>Mah, Su‐jung</au><au>Helms, Jill A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wnt Signaling Regulates Pulp Volume and Dentin Thickness</atitle><jtitle>Journal of bone and mineral research</jtitle><addtitle>J Bone Miner Res</addtitle><date>2014-04</date><risdate>2014</risdate><volume>29</volume><issue>4</issue><spage>892</spage><epage>901</epage><pages>892-901</pages><issn>0884-0431</issn><eissn>1523-4681</eissn><coden>JBMREJ</coden><abstract>ABSTRACT
Odontoblasts, cementoblasts, ameloblasts, and osteoblasts all form mineralized tissues in the craniofacial complex, and all these cell types exhibit active Wnt signaling during postnatal life. We set out to understand the functions of this Wnt signaling, by evaluating the phenotypes of mice in which the essential Wnt chaperone protein, Wntless was eliminated. The deletion of Wls was restricted to cells expressing Osteocalcin (OCN), which in addition to osteoblasts includes odontoblasts, cementoblasts, and ameloblasts. Dentin, cementum, enamel, and bone all formed in OCN‐Cre;Wlsfl/fl mice but their homeostasis was dramatically affected. The most notable feature was a significant increase in dentin volume and density. We attribute this gain in dentin volume to a Wnt‐mediated misregulation of Runx2. Normally, Wnt signaling stimulates Runx2, which in turn inhibits dentin sialoprotein (DSP); this inhibition must be relieved for odontoblasts to differentiate. In OCN‐Cre;Wlsfl/fl mice, Wnt pathway activation is reduced and Runx2 levels decline. The Runx2‐mediated repression of DSP is relieved and odontoblast differentiation is accordingly enhanced. This study demonstrates the importance of Wnt signaling in the homeostasis of mineralized tissues of the craniofacial complex. © 2014 American Society for Bone and Mineral Research.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>23996396</pmid><doi>10.1002/jbmr.2088</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0884-0431 |
| ispartof | Journal of bone and mineral research, 2014-04, Vol.29 (4), p.892-901 |
| issn | 0884-0431 1523-4681 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4541795 |
| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Core Binding Factor Alpha 1 Subunit - genetics Dental Pulp - anatomy & histology DENTIN Dentin - anatomy & histology Mice ODONTOBLAST PULP Real-Time Polymerase Chain Reaction Signal Transduction Wnt Proteins - metabolism WNT SIGNALING PATHWAY |
| title | Wnt Signaling Regulates Pulp Volume and Dentin Thickness |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T13%3A32%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Wnt%20Signaling%20Regulates%20Pulp%20Volume%20and%20Dentin%20Thickness&rft.jtitle=Journal%20of%20bone%20and%20mineral%20research&rft.au=Lim,%20Won%20Hee&rft.date=2014-04&rft.volume=29&rft.issue=4&rft.spage=892&rft.epage=901&rft.pages=892-901&rft.issn=0884-0431&rft.eissn=1523-4681&rft.coden=JBMREJ&rft_id=info:doi/10.1002/jbmr.2088&rft_dat=%3Cproquest_pubme%3E3249939131%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1508491496&rft_id=info:pmid/23996396&rfr_iscdi=true |