Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines
Puerarin has been widely used in clinical treatment and experiment research and is considered to exert an anticancer effect recently. The present study investigated the anticancer activity of puerarin in U251 and U87 human glioblastoma cells. The cells were treated with puerarin at various concentra...
Gespeichert in:
Veröffentlicht in: | International journal of clinical and experimental medicine 2015-01, Vol.8 (6), p.10132-10142 |
---|---|
Hauptverfasser: | , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 10142 |
---|---|
container_issue | 6 |
container_start_page | 10132 |
container_title | International journal of clinical and experimental medicine |
container_volume | 8 |
creator | Yang, Ji-An Li, Ji-Qiang Shao, Ling-Min Yang, Qian Liu, Bao-Hui Wu, Ting-Feng Wu, Peng Yi, Wei Chen, Qian-Xue |
description | Puerarin has been widely used in clinical treatment and experiment research and is considered to exert an anticancer effect recently. The present study investigated the anticancer activity of puerarin in U251 and U87 human glioblastoma cells. The cells were treated with puerarin at various concentrations for different times. Cell viability and cell proliferation were detected by cell counting kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) staining respectively. Cell cycle and apoptosis were measured separately with PI staining and Annexin V-FITC/PI double staining method by flow cytometry. DNA damage of glioblastoma cells caused by puerarin exposure was evaluated by γ-H2AX foci detection, and the expressions of p-AKT, caspase-3 and apoptosis-related proteins were detected by Western blotting after puerarin treatment. Cell viability and proliferation of glioblastoma cells treated with puerarin were significantly lower than that of the control group; the apoptosis rate increased obviously compared to the control group. Puerarin significantly decreased the proportion at G1 phase of cell cycling accompanied by increased populations at the S and G2/M phases in both cell lines. At the same time, DNA damage level of puerarin treated cells was significantly higher than that in the control cells. Moreover, puerarin treatment suppressed the expression of p-Akt and Bcl-2 and promoted the expression of Bax and cleaved caspase-3 in U251 cells. These findings indicate that puerarin exerts antitumor effects both in U251 and U87 cells. |
format | Article |
fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4538072</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1708163001</sourcerecordid><originalsourceid>FETCH-LOGICAL-p266t-2c6766acead5c023e04c61dd1f174a90b132246c02400005e96c7787ebdc012d3</originalsourceid><addsrcrecordid>eNpVUE1LAzEUDKLYWv0LkqOXhXzsJt2LIMUvKOhBvS5vk7SNZJM1yQr-eyNW0Xd5j5nHDDMHaE7bmlRNS-jhn3uGTlJ6JURQxttjNGOCk1ZSNkcvj5OJEK3H1u9sb3PCYwzObgqabfAYvC6UnpRJGMYw5pBsKgjeTQN4vHU29A5SDgNgZZzDznqTTtHRBlwyZ_u9QM8310-ru2r9cHu_ulpXIxMiV0wJKQQoA7pRhHFDaiWo1nRDZQ0t6SlnrBaFqkmZxrRCSbmUpteKUKb5Al1-645TPxitjM8RXDdGO0D86ALY7j_j7a7bhveubviSSFYELvYCMbxNJuVusOkrB3gTptRRSZa0tEVoeT3_6_Vr8lMm_wQiA3OI</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1708163001</pqid></control><display><type>article</type><title>Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines</title><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Yang, Ji-An ; Li, Ji-Qiang ; Shao, Ling-Min ; Yang, Qian ; Liu, Bao-Hui ; Wu, Ting-Feng ; Wu, Peng ; Yi, Wei ; Chen, Qian-Xue</creator><creatorcontrib>Yang, Ji-An ; Li, Ji-Qiang ; Shao, Ling-Min ; Yang, Qian ; Liu, Bao-Hui ; Wu, Ting-Feng ; Wu, Peng ; Yi, Wei ; Chen, Qian-Xue</creatorcontrib><description>Puerarin has been widely used in clinical treatment and experiment research and is considered to exert an anticancer effect recently. The present study investigated the anticancer activity of puerarin in U251 and U87 human glioblastoma cells. The cells were treated with puerarin at various concentrations for different times. Cell viability and cell proliferation were detected by cell counting kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) staining respectively. Cell cycle and apoptosis were measured separately with PI staining and Annexin V-FITC/PI double staining method by flow cytometry. DNA damage of glioblastoma cells caused by puerarin exposure was evaluated by γ-H2AX foci detection, and the expressions of p-AKT, caspase-3 and apoptosis-related proteins were detected by Western blotting after puerarin treatment. Cell viability and proliferation of glioblastoma cells treated with puerarin were significantly lower than that of the control group; the apoptosis rate increased obviously compared to the control group. Puerarin significantly decreased the proportion at G1 phase of cell cycling accompanied by increased populations at the S and G2/M phases in both cell lines. At the same time, DNA damage level of puerarin treated cells was significantly higher than that in the control cells. Moreover, puerarin treatment suppressed the expression of p-Akt and Bcl-2 and promoted the expression of Bax and cleaved caspase-3 in U251 cells. These findings indicate that puerarin exerts antitumor effects both in U251 and U87 cells.</description><identifier>ISSN: 1940-5901</identifier><identifier>EISSN: 1940-5901</identifier><identifier>PMID: 26309712</identifier><language>eng</language><publisher>United States: e-Century Publishing Corporation</publisher><subject>Original</subject><ispartof>International journal of clinical and experimental medicine, 2015-01, Vol.8 (6), p.10132-10142</ispartof><rights>IJCEM Copyright © 2015 2015</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4538072/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4538072/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26309712$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Ji-An</creatorcontrib><creatorcontrib>Li, Ji-Qiang</creatorcontrib><creatorcontrib>Shao, Ling-Min</creatorcontrib><creatorcontrib>Yang, Qian</creatorcontrib><creatorcontrib>Liu, Bao-Hui</creatorcontrib><creatorcontrib>Wu, Ting-Feng</creatorcontrib><creatorcontrib>Wu, Peng</creatorcontrib><creatorcontrib>Yi, Wei</creatorcontrib><creatorcontrib>Chen, Qian-Xue</creatorcontrib><title>Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines</title><title>International journal of clinical and experimental medicine</title><addtitle>Int J Clin Exp Med</addtitle><description>Puerarin has been widely used in clinical treatment and experiment research and is considered to exert an anticancer effect recently. The present study investigated the anticancer activity of puerarin in U251 and U87 human glioblastoma cells. The cells were treated with puerarin at various concentrations for different times. Cell viability and cell proliferation were detected by cell counting kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) staining respectively. Cell cycle and apoptosis were measured separately with PI staining and Annexin V-FITC/PI double staining method by flow cytometry. DNA damage of glioblastoma cells caused by puerarin exposure was evaluated by γ-H2AX foci detection, and the expressions of p-AKT, caspase-3 and apoptosis-related proteins were detected by Western blotting after puerarin treatment. Cell viability and proliferation of glioblastoma cells treated with puerarin were significantly lower than that of the control group; the apoptosis rate increased obviously compared to the control group. Puerarin significantly decreased the proportion at G1 phase of cell cycling accompanied by increased populations at the S and G2/M phases in both cell lines. At the same time, DNA damage level of puerarin treated cells was significantly higher than that in the control cells. Moreover, puerarin treatment suppressed the expression of p-Akt and Bcl-2 and promoted the expression of Bax and cleaved caspase-3 in U251 cells. These findings indicate that puerarin exerts antitumor effects both in U251 and U87 cells.</description><subject>Original</subject><issn>1940-5901</issn><issn>1940-5901</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNpVUE1LAzEUDKLYWv0LkqOXhXzsJt2LIMUvKOhBvS5vk7SNZJM1yQr-eyNW0Xd5j5nHDDMHaE7bmlRNS-jhn3uGTlJ6JURQxttjNGOCk1ZSNkcvj5OJEK3H1u9sb3PCYwzObgqabfAYvC6UnpRJGMYw5pBsKgjeTQN4vHU29A5SDgNgZZzDznqTTtHRBlwyZ_u9QM8310-ru2r9cHu_ulpXIxMiV0wJKQQoA7pRhHFDaiWo1nRDZQ0t6SlnrBaFqkmZxrRCSbmUpteKUKb5Al1-645TPxitjM8RXDdGO0D86ALY7j_j7a7bhveubviSSFYELvYCMbxNJuVusOkrB3gTptRRSZa0tEVoeT3_6_Vr8lMm_wQiA3OI</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Yang, Ji-An</creator><creator>Li, Ji-Qiang</creator><creator>Shao, Ling-Min</creator><creator>Yang, Qian</creator><creator>Liu, Bao-Hui</creator><creator>Wu, Ting-Feng</creator><creator>Wu, Peng</creator><creator>Yi, Wei</creator><creator>Chen, Qian-Xue</creator><general>e-Century Publishing Corporation</general><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines</title><author>Yang, Ji-An ; Li, Ji-Qiang ; Shao, Ling-Min ; Yang, Qian ; Liu, Bao-Hui ; Wu, Ting-Feng ; Wu, Peng ; Yi, Wei ; Chen, Qian-Xue</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p266t-2c6766acead5c023e04c61dd1f174a90b132246c02400005e96c7787ebdc012d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Original</topic><toplevel>online_resources</toplevel><creatorcontrib>Yang, Ji-An</creatorcontrib><creatorcontrib>Li, Ji-Qiang</creatorcontrib><creatorcontrib>Shao, Ling-Min</creatorcontrib><creatorcontrib>Yang, Qian</creatorcontrib><creatorcontrib>Liu, Bao-Hui</creatorcontrib><creatorcontrib>Wu, Ting-Feng</creatorcontrib><creatorcontrib>Wu, Peng</creatorcontrib><creatorcontrib>Yi, Wei</creatorcontrib><creatorcontrib>Chen, Qian-Xue</creatorcontrib><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of clinical and experimental medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Ji-An</au><au>Li, Ji-Qiang</au><au>Shao, Ling-Min</au><au>Yang, Qian</au><au>Liu, Bao-Hui</au><au>Wu, Ting-Feng</au><au>Wu, Peng</au><au>Yi, Wei</au><au>Chen, Qian-Xue</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines</atitle><jtitle>International journal of clinical and experimental medicine</jtitle><addtitle>Int J Clin Exp Med</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>8</volume><issue>6</issue><spage>10132</spage><epage>10142</epage><pages>10132-10142</pages><issn>1940-5901</issn><eissn>1940-5901</eissn><abstract>Puerarin has been widely used in clinical treatment and experiment research and is considered to exert an anticancer effect recently. The present study investigated the anticancer activity of puerarin in U251 and U87 human glioblastoma cells. The cells were treated with puerarin at various concentrations for different times. Cell viability and cell proliferation were detected by cell counting kit-8 (CCK-8) assay and 5-ethynyl-2'-deoxyuridine (EdU) staining respectively. Cell cycle and apoptosis were measured separately with PI staining and Annexin V-FITC/PI double staining method by flow cytometry. DNA damage of glioblastoma cells caused by puerarin exposure was evaluated by γ-H2AX foci detection, and the expressions of p-AKT, caspase-3 and apoptosis-related proteins were detected by Western blotting after puerarin treatment. Cell viability and proliferation of glioblastoma cells treated with puerarin were significantly lower than that of the control group; the apoptosis rate increased obviously compared to the control group. Puerarin significantly decreased the proportion at G1 phase of cell cycling accompanied by increased populations at the S and G2/M phases in both cell lines. At the same time, DNA damage level of puerarin treated cells was significantly higher than that in the control cells. Moreover, puerarin treatment suppressed the expression of p-Akt and Bcl-2 and promoted the expression of Bax and cleaved caspase-3 in U251 cells. These findings indicate that puerarin exerts antitumor effects both in U251 and U87 cells.</abstract><cop>United States</cop><pub>e-Century Publishing Corporation</pub><pmid>26309712</pmid><tpages>11</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1940-5901 |
ispartof | International journal of clinical and experimental medicine, 2015-01, Vol.8 (6), p.10132-10142 |
issn | 1940-5901 1940-5901 |
language | eng |
recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4538072 |
source | EZB-FREE-00999 freely available EZB journals; PubMed Central |
subjects | Original |
title | Puerarin inhibits proliferation and induces apoptosis in human glioblastoma cell lines |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T03%3A04%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Puerarin%20inhibits%20proliferation%20and%20induces%20apoptosis%20in%20human%20glioblastoma%20cell%20lines&rft.jtitle=International%20journal%20of%20clinical%20and%20experimental%20medicine&rft.au=Yang,%20Ji-An&rft.date=2015-01-01&rft.volume=8&rft.issue=6&rft.spage=10132&rft.epage=10142&rft.pages=10132-10142&rft.issn=1940-5901&rft.eissn=1940-5901&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E1708163001%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1708163001&rft_id=info:pmid/26309712&rfr_iscdi=true |