Development of a novel imaging system for cell therapy in the brain
Stem cells have been evaluated as a potential therapeutic approach for several neurological disorders of the central and peripheral nervous system as well as for traumatic brain and spinal cord injury. Currently, the lack of a reliable and safe method to accurately and non-invasively locate the site...
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| Veröffentlicht in: | Stem cell research & therapy 2015-07, Vol.6 (1), p.131-131, Article 131 |
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| creator | Micci, Maria-Adelaide Boone, Debbie R Parsley, Margaret A Wei, Jingna Patrikeev, Igor Motamedi, Massoud Hellmich, Helen L |
| description | Stem cells have been evaluated as a potential therapeutic approach for several neurological disorders of the central and peripheral nervous system as well as for traumatic brain and spinal cord injury. Currently, the lack of a reliable and safe method to accurately and non-invasively locate the site of implantation and track the migration of stem cells in vivo hampers the development of stem cell therapy and its clinical application. In this report, we present data that demonstrate the feasibility of using the human sodium iodide symporter (hNIS) as a reporter gene for tracking neural stem cells (NSCs) after transplantation in the brain by using single-photon emission tomography/computed tomography (SPECT/CT) imaging.
NSCs were isolated from the hippocampus of adult rats (Hipp-NSCs) and transduced with a lentiviral vector containing the hNIS gene. Hipp-NSCs expressing the hNIS (NIS-Hipp-NSCs) were characterized in vitro and in vivo after transplantation in the rat brain and imaged by using technetium-99m ((99m)Tc) and a small rodent SPECT/CT apparatus. Comparisons were made between Hipp-NSCs and NIS-Hipp-NSCs, and statistical analysis was performed by using two-tailed Student's t test.
Our results show that the expression of the hNIS allows the repeated visualization of NSCs in vivo in the brain by using SPECT/CT imaging and does not affect the ability of Hipp-NSCs to generate neuronal and glial cells in vitro and in vivo.
These data support the use of the hNIS as a reporter gene for non-invasive imaging of NSCs in the brain. The repeated, non-invasive tracking of implanted cells will accelerate the development of effective stem cell therapies for traumatic brain injury and other types of central nervous system injury. |
| doi_str_mv | 10.1186/s13287-015-0129-7 |
| format | Article |
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NSCs were isolated from the hippocampus of adult rats (Hipp-NSCs) and transduced with a lentiviral vector containing the hNIS gene. Hipp-NSCs expressing the hNIS (NIS-Hipp-NSCs) were characterized in vitro and in vivo after transplantation in the rat brain and imaged by using technetium-99m ((99m)Tc) and a small rodent SPECT/CT apparatus. Comparisons were made between Hipp-NSCs and NIS-Hipp-NSCs, and statistical analysis was performed by using two-tailed Student's t test.
Our results show that the expression of the hNIS allows the repeated visualization of NSCs in vivo in the brain by using SPECT/CT imaging and does not affect the ability of Hipp-NSCs to generate neuronal and glial cells in vitro and in vivo.
These data support the use of the hNIS as a reporter gene for non-invasive imaging of NSCs in the brain. The repeated, non-invasive tracking of implanted cells will accelerate the development of effective stem cell therapies for traumatic brain injury and other types of central nervous system injury.</description><identifier>ISSN: 1757-6512</identifier><identifier>EISSN: 1757-6512</identifier><identifier>DOI: 10.1186/s13287-015-0129-7</identifier><identifier>PMID: 26194790</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; Blotting, Western ; Brain ; Brain - pathology ; Care and treatment ; Cell Proliferation - physiology ; Cell- and Tissue-Based Therapy - methods ; Cells, Cultured ; Diagnostic Imaging - methods ; Genetic vectors ; Health aspects ; Hippocampus - cytology ; Hippocampus - metabolism ; Injuries ; Male ; Nervous system diseases ; Neural Stem Cells - physiology ; Neurons ; Physiological aspects ; Rats ; Rats, Sprague-Dawley ; Spinal cord injuries ; Stem cells ; Tomography, Emission-Computed, Single-Photon ; Transplantation</subject><ispartof>Stem cell research & therapy, 2015-07, Vol.6 (1), p.131-131, Article 131</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2015</rights><rights>Micci et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c559t-7c8daee3446cbd5abbe18ffe38fac4d6d9ea41cdb0a025e0e6b39167679a03073</citedby><cites>FETCH-LOGICAL-c559t-7c8daee3446cbd5abbe18ffe38fac4d6d9ea41cdb0a025e0e6b39167679a03073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4534109/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4534109/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26194790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Micci, Maria-Adelaide</creatorcontrib><creatorcontrib>Boone, Debbie R</creatorcontrib><creatorcontrib>Parsley, Margaret A</creatorcontrib><creatorcontrib>Wei, Jingna</creatorcontrib><creatorcontrib>Patrikeev, Igor</creatorcontrib><creatorcontrib>Motamedi, Massoud</creatorcontrib><creatorcontrib>Hellmich, Helen L</creatorcontrib><title>Development of a novel imaging system for cell therapy in the brain</title><title>Stem cell research & therapy</title><addtitle>Stem Cell Res Ther</addtitle><description>Stem cells have been evaluated as a potential therapeutic approach for several neurological disorders of the central and peripheral nervous system as well as for traumatic brain and spinal cord injury. Currently, the lack of a reliable and safe method to accurately and non-invasively locate the site of implantation and track the migration of stem cells in vivo hampers the development of stem cell therapy and its clinical application. In this report, we present data that demonstrate the feasibility of using the human sodium iodide symporter (hNIS) as a reporter gene for tracking neural stem cells (NSCs) after transplantation in the brain by using single-photon emission tomography/computed tomography (SPECT/CT) imaging.
NSCs were isolated from the hippocampus of adult rats (Hipp-NSCs) and transduced with a lentiviral vector containing the hNIS gene. Hipp-NSCs expressing the hNIS (NIS-Hipp-NSCs) were characterized in vitro and in vivo after transplantation in the rat brain and imaged by using technetium-99m ((99m)Tc) and a small rodent SPECT/CT apparatus. Comparisons were made between Hipp-NSCs and NIS-Hipp-NSCs, and statistical analysis was performed by using two-tailed Student's t test.
Our results show that the expression of the hNIS allows the repeated visualization of NSCs in vivo in the brain by using SPECT/CT imaging and does not affect the ability of Hipp-NSCs to generate neuronal and glial cells in vitro and in vivo.
These data support the use of the hNIS as a reporter gene for non-invasive imaging of NSCs in the brain. The repeated, non-invasive tracking of implanted cells will accelerate the development of effective stem cell therapies for traumatic brain injury and other types of central nervous system injury.</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>Brain</subject><subject>Brain - pathology</subject><subject>Care and treatment</subject><subject>Cell Proliferation - physiology</subject><subject>Cell- and Tissue-Based Therapy - methods</subject><subject>Cells, Cultured</subject><subject>Diagnostic Imaging - methods</subject><subject>Genetic vectors</subject><subject>Health aspects</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - metabolism</subject><subject>Injuries</subject><subject>Male</subject><subject>Nervous system diseases</subject><subject>Neural Stem Cells - physiology</subject><subject>Neurons</subject><subject>Physiological aspects</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spinal cord injuries</subject><subject>Stem cells</subject><subject>Tomography, Emission-Computed, Single-Photon</subject><subject>Transplantation</subject><issn>1757-6512</issn><issn>1757-6512</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkm9r3SAUxmVsrKXrB9ibIQzG-iKdxqjJm0G567ZCYbA_r8WYY64l0TtNyu63n-F23c2YIh70d55zlAehl5RcUlqLd4myspYFoTyvsinkE3RKJZeF4LR8ehSfoPOU7kgejBEiqufopBS0qWRDTtHmA9zDEHYj-AkHizX2IR9gN-re-R6nfZpgxDZEbGAY8LSFqHd77PwS4jZq51-gZ1YPCc4f9jP04-P1983n4vbLp5vN1W1hOG-mQpq60wCsqoRpO67bFmhtLbDaalN1omtAV9R0LdGk5EBAtKyhQgrZaMKIZGfo_UF3N7cjdCa3HPWgdjE3G_cqaKfWN95tVR_uVcVZRUmTBd4-CMTwc4Y0qdGl5VnaQ5iTopIwWZJcMaOv_0Hvwhx9fl6mZMkJKWX9l-r1AMp5G3Jds4iqK15RwXhJF63L_1B5djA6EzxYl89XCRerhMxM8Gvq9ZySuvn2dc2-OWK3oIdpm8IwTy74tAbpATQxpBTBPn4cJWpxlDo4SmVHqcVRasl5dfzjjxl__MN-A5a-w2c</recordid><startdate>20150721</startdate><enddate>20150721</enddate><creator>Micci, Maria-Adelaide</creator><creator>Boone, Debbie R</creator><creator>Parsley, Margaret A</creator><creator>Wei, Jingna</creator><creator>Patrikeev, Igor</creator><creator>Motamedi, Massoud</creator><creator>Hellmich, Helen L</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150721</creationdate><title>Development of a novel imaging system for cell therapy in the brain</title><author>Micci, Maria-Adelaide ; 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Currently, the lack of a reliable and safe method to accurately and non-invasively locate the site of implantation and track the migration of stem cells in vivo hampers the development of stem cell therapy and its clinical application. In this report, we present data that demonstrate the feasibility of using the human sodium iodide symporter (hNIS) as a reporter gene for tracking neural stem cells (NSCs) after transplantation in the brain by using single-photon emission tomography/computed tomography (SPECT/CT) imaging.
NSCs were isolated from the hippocampus of adult rats (Hipp-NSCs) and transduced with a lentiviral vector containing the hNIS gene. Hipp-NSCs expressing the hNIS (NIS-Hipp-NSCs) were characterized in vitro and in vivo after transplantation in the rat brain and imaged by using technetium-99m ((99m)Tc) and a small rodent SPECT/CT apparatus. Comparisons were made between Hipp-NSCs and NIS-Hipp-NSCs, and statistical analysis was performed by using two-tailed Student's t test.
Our results show that the expression of the hNIS allows the repeated visualization of NSCs in vivo in the brain by using SPECT/CT imaging and does not affect the ability of Hipp-NSCs to generate neuronal and glial cells in vitro and in vivo.
These data support the use of the hNIS as a reporter gene for non-invasive imaging of NSCs in the brain. The repeated, non-invasive tracking of implanted cells will accelerate the development of effective stem cell therapies for traumatic brain injury and other types of central nervous system injury.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26194790</pmid><doi>10.1186/s13287-015-0129-7</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Blotting, Western Brain Brain - pathology Care and treatment Cell Proliferation - physiology Cell- and Tissue-Based Therapy - methods Cells, Cultured Diagnostic Imaging - methods Genetic vectors Health aspects Hippocampus - cytology Hippocampus - metabolism Injuries Male Nervous system diseases Neural Stem Cells - physiology Neurons Physiological aspects Rats Rats, Sprague-Dawley Spinal cord injuries Stem cells Tomography, Emission-Computed, Single-Photon Transplantation |
| title | Development of a novel imaging system for cell therapy in the brain |
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