Effect of the synthetic cannabinoid HU-210 on quorum sensing and on the production of quorum sensing-mediated virulence factors by Vibrio harveyi
Bacterial populations communicate through the cell density-dependent mechanism of quorum sensing (QS). Vibrio harveyi, one of the best studied model organisms for QS, was used to explore effects of the synthetic cannabinoid HU-210 on QS and different QS-regulated physiological processes in bacteria....
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| Veröffentlicht in: | BMC microbiology 2015-08, Vol.15 (1), p.159-159, Article 159 |
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| creator | Soni, Divya Smoum, Reem Breuer, Aviva Mechoulam, Raphael Steinberg, Doron |
| description | Bacterial populations communicate through the cell density-dependent mechanism of quorum sensing (QS). Vibrio harveyi, one of the best studied model organisms for QS, was used to explore effects of the synthetic cannabinoid HU-210 on QS and different QS-regulated physiological processes in bacteria.
Analysis of QS-regulated bioluminescence in wild-type and mutant strains of V. harveyi revealed that HU-210 affects the autoinducer-2 (AI-2) pathway, one of three known QS cascades of V. harveyi. Furthermore, QS-mediated biofilm formation and swimming motility in the mutant strain BB152 (AI-1(-), AI-2(+)) were significantly reduced in the presence of HU-210. HU-210 inhibited QS-mediated virulence factor production without any inhibitory effect on bacterial growth. It also alters the expression of several genes, which are regulated by QS, specifically downregulating the genes of the AI-2 QS cascade.
First evidence is being provided for interference of bacterial signal-transduction systems by a synthetic cannabinoid. The effect of HU-210 was specific to the AI-2 cascade in V. harveyi. AI-2 is known as a "universal autoinducer" and interference with its activity opens a broad spectrum of applications for synthetic cannabinoids in future research as a potential anti-QS agent. |
| doi_str_mv | 10.1186/s12866-015-0499-0 |
| format | Article |
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Analysis of QS-regulated bioluminescence in wild-type and mutant strains of V. harveyi revealed that HU-210 affects the autoinducer-2 (AI-2) pathway, one of three known QS cascades of V. harveyi. Furthermore, QS-mediated biofilm formation and swimming motility in the mutant strain BB152 (AI-1(-), AI-2(+)) were significantly reduced in the presence of HU-210. HU-210 inhibited QS-mediated virulence factor production without any inhibitory effect on bacterial growth. It also alters the expression of several genes, which are regulated by QS, specifically downregulating the genes of the AI-2 QS cascade.
First evidence is being provided for interference of bacterial signal-transduction systems by a synthetic cannabinoid. The effect of HU-210 was specific to the AI-2 cascade in V. harveyi. AI-2 is known as a "universal autoinducer" and interference with its activity opens a broad spectrum of applications for synthetic cannabinoids in future research as a potential anti-QS agent.</description><identifier>ISSN: 1471-2180</identifier><identifier>EISSN: 1471-2180</identifier><identifier>DOI: 10.1186/s12866-015-0499-0</identifier><identifier>PMID: 26264142</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Bacteria ; Biofilms ; Cannabinoids - metabolism ; Dronabinol - analogs & derivatives ; Dronabinol - metabolism ; Gene expression ; Gene Expression Regulation, Bacterial - drug effects ; Homoserine - analogs & derivatives ; Homoserine - metabolism ; Lactones - metabolism ; Quorum Sensing - drug effects ; Signal transduction ; Signal Transduction - drug effects ; Vibrio - drug effects ; Vibrio - metabolism ; Virulence ; Virulence Factors - biosynthesis</subject><ispartof>BMC microbiology, 2015-08, Vol.15 (1), p.159-159, Article 159</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Copyright BioMed Central 2015</rights><rights>Soni et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-2195986f3c9b2b98bf32d4634d1983fce5263617e248826f17c3ef234db23f2e3</citedby><cites>FETCH-LOGICAL-c528t-2195986f3c9b2b98bf32d4634d1983fce5263617e248826f17c3ef234db23f2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531395/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4531395/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26264142$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Soni, Divya</creatorcontrib><creatorcontrib>Smoum, Reem</creatorcontrib><creatorcontrib>Breuer, Aviva</creatorcontrib><creatorcontrib>Mechoulam, Raphael</creatorcontrib><creatorcontrib>Steinberg, Doron</creatorcontrib><title>Effect of the synthetic cannabinoid HU-210 on quorum sensing and on the production of quorum sensing-mediated virulence factors by Vibrio harveyi</title><title>BMC microbiology</title><addtitle>BMC Microbiol</addtitle><description>Bacterial populations communicate through the cell density-dependent mechanism of quorum sensing (QS). Vibrio harveyi, one of the best studied model organisms for QS, was used to explore effects of the synthetic cannabinoid HU-210 on QS and different QS-regulated physiological processes in bacteria.
Analysis of QS-regulated bioluminescence in wild-type and mutant strains of V. harveyi revealed that HU-210 affects the autoinducer-2 (AI-2) pathway, one of three known QS cascades of V. harveyi. Furthermore, QS-mediated biofilm formation and swimming motility in the mutant strain BB152 (AI-1(-), AI-2(+)) were significantly reduced in the presence of HU-210. HU-210 inhibited QS-mediated virulence factor production without any inhibitory effect on bacterial growth. It also alters the expression of several genes, which are regulated by QS, specifically downregulating the genes of the AI-2 QS cascade.
First evidence is being provided for interference of bacterial signal-transduction systems by a synthetic cannabinoid. The effect of HU-210 was specific to the AI-2 cascade in V. harveyi. AI-2 is known as a "universal autoinducer" and interference with its activity opens a broad spectrum of applications for synthetic cannabinoids in future research as a potential anti-QS agent.</description><subject>Bacteria</subject><subject>Biofilms</subject><subject>Cannabinoids - metabolism</subject><subject>Dronabinol - analogs & derivatives</subject><subject>Dronabinol - metabolism</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial - drug effects</subject><subject>Homoserine - analogs & derivatives</subject><subject>Homoserine - metabolism</subject><subject>Lactones - metabolism</subject><subject>Quorum Sensing - drug effects</subject><subject>Signal transduction</subject><subject>Signal Transduction - drug effects</subject><subject>Vibrio - drug effects</subject><subject>Vibrio - metabolism</subject><subject>Virulence</subject><subject>Virulence Factors - biosynthesis</subject><issn>1471-2180</issn><issn>1471-2180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptks1u1DAUhSMEoqXwAGyQJTawSPFP4tgbpKoqtFIlJKBsLce5nnGVsae2M2IegzfG0ZTSIOSF7evvHP-dqnpN8Ckhgn9IhArOa0zaGjdS1vhJdUyajtSUCPz00fioepHSLcakE6x7Xh1RTnlDGnpc_bqwFkxGwaK8BpT2vnTZGWS097p3PrgBXd4UF4yCR3dTiNMGJfDJ-RXSfpirs3IbwzCZ7Mq0eC25egOD0xkGtHNxGsEbQFabHGJC_R79cH10Aa113MHevayeWT0meHXfn1Q3ny6-n1_W118-X52fXdempSKXA8lWCm6ZkT3tpegto0PDWTMQKZg10FLOOOmANkJQbklnGFha1nvKLAV2Un08-G6nvpzPgM9Rj2ob3UbHvQraqeWKd2u1CjvVtIww2RaDd_cGMdxNkLLauGRgHLWHMCVFOsy4lOVjCvr2H_Q2TNGX6xVK4PKXRJK_1EqPoJy3oexrZlN11jaEdRSzmTr9D1XaABtnggfrSn0heL8QFCbDz7zSU0rq6tvXJUsOrIkhpQj24T0IVnPm1CFzqmROzZlTuGjePH7IB8WfkLHfnqnROg</recordid><startdate>20150812</startdate><enddate>20150812</enddate><creator>Soni, Divya</creator><creator>Smoum, Reem</creator><creator>Breuer, Aviva</creator><creator>Mechoulam, Raphael</creator><creator>Steinberg, Doron</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150812</creationdate><title>Effect of the synthetic cannabinoid HU-210 on quorum sensing and on the production of quorum sensing-mediated virulence factors by Vibrio harveyi</title><author>Soni, Divya ; Smoum, Reem ; Breuer, Aviva ; Mechoulam, Raphael ; Steinberg, Doron</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-2195986f3c9b2b98bf32d4634d1983fce5263617e248826f17c3ef234db23f2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bacteria</topic><topic>Biofilms</topic><topic>Cannabinoids - metabolism</topic><topic>Dronabinol - analogs & derivatives</topic><topic>Dronabinol - metabolism</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial - drug effects</topic><topic>Homoserine - analogs & derivatives</topic><topic>Homoserine - metabolism</topic><topic>Lactones - metabolism</topic><topic>Quorum Sensing - drug effects</topic><topic>Signal transduction</topic><topic>Signal Transduction - drug effects</topic><topic>Vibrio - drug effects</topic><topic>Vibrio - metabolism</topic><topic>Virulence</topic><topic>Virulence Factors - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Soni, Divya</creatorcontrib><creatorcontrib>Smoum, Reem</creatorcontrib><creatorcontrib>Breuer, Aviva</creatorcontrib><creatorcontrib>Mechoulam, Raphael</creatorcontrib><creatorcontrib>Steinberg, Doron</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Soni, Divya</au><au>Smoum, Reem</au><au>Breuer, Aviva</au><au>Mechoulam, Raphael</au><au>Steinberg, Doron</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of the synthetic cannabinoid HU-210 on quorum sensing and on the production of quorum sensing-mediated virulence factors by Vibrio harveyi</atitle><jtitle>BMC microbiology</jtitle><addtitle>BMC Microbiol</addtitle><date>2015-08-12</date><risdate>2015</risdate><volume>15</volume><issue>1</issue><spage>159</spage><epage>159</epage><pages>159-159</pages><artnum>159</artnum><issn>1471-2180</issn><eissn>1471-2180</eissn><abstract>Bacterial populations communicate through the cell density-dependent mechanism of quorum sensing (QS). Vibrio harveyi, one of the best studied model organisms for QS, was used to explore effects of the synthetic cannabinoid HU-210 on QS and different QS-regulated physiological processes in bacteria.
Analysis of QS-regulated bioluminescence in wild-type and mutant strains of V. harveyi revealed that HU-210 affects the autoinducer-2 (AI-2) pathway, one of three known QS cascades of V. harveyi. Furthermore, QS-mediated biofilm formation and swimming motility in the mutant strain BB152 (AI-1(-), AI-2(+)) were significantly reduced in the presence of HU-210. HU-210 inhibited QS-mediated virulence factor production without any inhibitory effect on bacterial growth. It also alters the expression of several genes, which are regulated by QS, specifically downregulating the genes of the AI-2 QS cascade.
First evidence is being provided for interference of bacterial signal-transduction systems by a synthetic cannabinoid. The effect of HU-210 was specific to the AI-2 cascade in V. harveyi. AI-2 is known as a "universal autoinducer" and interference with its activity opens a broad spectrum of applications for synthetic cannabinoids in future research as a potential anti-QS agent.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26264142</pmid><doi>10.1186/s12866-015-0499-0</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; SpringerNature Journals; PubMed Central Open Access; Springer Nature OA Free Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Bacteria Biofilms Cannabinoids - metabolism Dronabinol - analogs & derivatives Dronabinol - metabolism Gene expression Gene Expression Regulation, Bacterial - drug effects Homoserine - analogs & derivatives Homoserine - metabolism Lactones - metabolism Quorum Sensing - drug effects Signal transduction Signal Transduction - drug effects Vibrio - drug effects Vibrio - metabolism Virulence Virulence Factors - biosynthesis |
| title | Effect of the synthetic cannabinoid HU-210 on quorum sensing and on the production of quorum sensing-mediated virulence factors by Vibrio harveyi |
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