Ligand-Induced Proton Transfer and Low-Barrier Hydrogen Bond Revealed by X‑ray Crystallography

Ligand binding can change the pK a of protein residues and influence enzyme catalysis. Herein, we report three ultrahigh resolution X-ray crystal structures of CTX-M β-lactamase, directly visualizing protonation state changes along the enzymatic pathway: apo protein at 0.79 Å, precovalent complex wi...

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