Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin
Contact with Leishmania leads to a decreases in mononuclear phagocyte adherence to connective tissue. In this work, we studied the early stages of bond formation between VLA4 and fibronectin, measured the kinetics of membrane alignment and the monocyte cytoplasm spreading area over a fibronectin-coa...
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creator | Figueira, Cláudio Pereira Carvalhal, Djalma Gomes Ferrão Almeida, Rafaela Andrade Hermida, Micely d’ El-Rei Touchard, Dominique Robert, Phillipe Pierres, Anne Bongrand, Pierre dos-Santos, Washington LC |
description | Contact with
Leishmania
leads to a decreases in mononuclear phagocyte adherence to connective tissue. In this work, we studied the early stages of bond formation between VLA4 and fibronectin, measured the kinetics of membrane alignment and the monocyte cytoplasm spreading area over a fibronectin-coated surface and studied the expression of high affinity integrin epitope in uninfected and
Leishmania
-infected human monocytes. Our results show that the initial VLA4-mediated interaction of
Leishmania
-infected monocyte with a fibronectin-coated surface is preserved, however, the later stage, leukocyte spreading over the substrate is abrogated in
Leishmania
-infected cells. The median of spreading area was 72 [55–89] μm
2
for uninfected and 41 [34–51] μm
2
for
Leishmania
-infected monocyte. This cytoplasm spread was inhibited using an anti-VLA4 blocking antibody. After the initial contact with the fibronectrin-coated surface, uninfected monocyte quickly spread the cytoplasm at a 15 μm
2
s
−1
ratio whilst
Leishmania
-infected monocytes only made small contacts at a 5.5 μm
2
s
−1
ratio. The expression of high affinity epitope by VLA4 (from 39 ± 21% to 14 ± 3%); and LFA1 (from 37 ± 32% to 18 ± 16%) molecules was reduced in
Leishmania
-infected monocytes. These changes in phagocyte function may be important for parasite dissemination and distribution of lesions in leishmaniasis. |
doi_str_mv | 10.1038/srep12862 |
format | Article |
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Leishmania
leads to a decreases in mononuclear phagocyte adherence to connective tissue. In this work, we studied the early stages of bond formation between VLA4 and fibronectin, measured the kinetics of membrane alignment and the monocyte cytoplasm spreading area over a fibronectin-coated surface and studied the expression of high affinity integrin epitope in uninfected and
Leishmania
-infected human monocytes. Our results show that the initial VLA4-mediated interaction of
Leishmania
-infected monocyte with a fibronectin-coated surface is preserved, however, the later stage, leukocyte spreading over the substrate is abrogated in
Leishmania
-infected cells. The median of spreading area was 72 [55–89] μm
2
for uninfected and 41 [34–51] μm
2
for
Leishmania
-infected monocyte. This cytoplasm spread was inhibited using an anti-VLA4 blocking antibody. After the initial contact with the fibronectrin-coated surface, uninfected monocyte quickly spread the cytoplasm at a 15 μm
2
s
−1
ratio whilst
Leishmania
-infected monocytes only made small contacts at a 5.5 μm
2
s
−1
ratio. The expression of high affinity epitope by VLA4 (from 39 ± 21% to 14 ± 3%); and LFA1 (from 37 ± 32% to 18 ± 16%) molecules was reduced in
Leishmania
-infected monocytes. These changes in phagocyte function may be important for parasite dissemination and distribution of lesions in leishmaniasis.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep12862</identifier><identifier>PMID: 26249106</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14 ; 14/28 ; 14/63 ; 631/326/417/2546 ; 631/80/79/1236 ; Affinity ; Cell activation ; Cell Adhesion - physiology ; Cytoplasm ; Epitopes ; Fibronectin ; Fibronectins - metabolism ; Humanities and Social Sciences ; Humans ; Integrin alpha4beta1 - metabolism ; Integrin beta1 - metabolism ; Kinetics ; Leishmania - metabolism ; Leishmaniasis ; Leishmaniasis - metabolism ; Leishmaniasis - parasitology ; Leukocytes - metabolism ; Leukocytes - parasitology ; Life Sciences ; Monocytes ; Monocytes - metabolism ; Monocytes - parasitology ; multidisciplinary ; Parasites ; Science ; Spreading ; Vector-borne diseases</subject><ispartof>Scientific reports, 2015-08, Vol.5 (1), p.12862-12862, Article 12862</ispartof><rights>The Author(s) 2015</rights><rights>Copyright Nature Publishing Group Aug 2015</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Copyright © 2015, Macmillan Publishers Limited 2015 Macmillan Publishers Limited</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-90036aae7d661322073dff2c6e244d565eb9b192ba5128b784f8d3392593e5243</citedby><cites>FETCH-LOGICAL-c538t-90036aae7d661322073dff2c6e244d565eb9b192ba5128b784f8d3392593e5243</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528201/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4528201/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26249106$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://amu.hal.science/hal-01198769$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Figueira, Cláudio Pereira</creatorcontrib><creatorcontrib>Carvalhal, Djalma Gomes Ferrão</creatorcontrib><creatorcontrib>Almeida, Rafaela Andrade</creatorcontrib><creatorcontrib>Hermida, Micely d’ El-Rei</creatorcontrib><creatorcontrib>Touchard, Dominique</creatorcontrib><creatorcontrib>Robert, Phillipe</creatorcontrib><creatorcontrib>Pierres, Anne</creatorcontrib><creatorcontrib>Bongrand, Pierre</creatorcontrib><creatorcontrib>dos-Santos, Washington LC</creatorcontrib><title>Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Contact with
Leishmania
leads to a decreases in mononuclear phagocyte adherence to connective tissue. In this work, we studied the early stages of bond formation between VLA4 and fibronectin, measured the kinetics of membrane alignment and the monocyte cytoplasm spreading area over a fibronectin-coated surface and studied the expression of high affinity integrin epitope in uninfected and
Leishmania
-infected human monocytes. Our results show that the initial VLA4-mediated interaction of
Leishmania
-infected monocyte with a fibronectin-coated surface is preserved, however, the later stage, leukocyte spreading over the substrate is abrogated in
Leishmania
-infected cells. The median of spreading area was 72 [55–89] μm
2
for uninfected and 41 [34–51] μm
2
for
Leishmania
-infected monocyte. This cytoplasm spread was inhibited using an anti-VLA4 blocking antibody. After the initial contact with the fibronectrin-coated surface, uninfected monocyte quickly spread the cytoplasm at a 15 μm
2
s
−1
ratio whilst
Leishmania
-infected monocytes only made small contacts at a 5.5 μm
2
s
−1
ratio. The expression of high affinity epitope by VLA4 (from 39 ± 21% to 14 ± 3%); and LFA1 (from 37 ± 32% to 18 ± 16%) molecules was reduced in
Leishmania
-infected monocytes. These changes in phagocyte function may be important for parasite dissemination and distribution of lesions in leishmaniasis.</description><subject>14</subject><subject>14/28</subject><subject>14/63</subject><subject>631/326/417/2546</subject><subject>631/80/79/1236</subject><subject>Affinity</subject><subject>Cell activation</subject><subject>Cell Adhesion - physiology</subject><subject>Cytoplasm</subject><subject>Epitopes</subject><subject>Fibronectin</subject><subject>Fibronectins - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Integrin alpha4beta1 - metabolism</subject><subject>Integrin beta1 - metabolism</subject><subject>Kinetics</subject><subject>Leishmania - metabolism</subject><subject>Leishmaniasis</subject><subject>Leishmaniasis - metabolism</subject><subject>Leishmaniasis - parasitology</subject><subject>Leukocytes - metabolism</subject><subject>Leukocytes - parasitology</subject><subject>Life Sciences</subject><subject>Monocytes</subject><subject>Monocytes - metabolism</subject><subject>Monocytes - parasitology</subject><subject>multidisciplinary</subject><subject>Parasites</subject><subject>Science</subject><subject>Spreading</subject><subject>Vector-borne diseases</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNplkU9v1DAQxS0EolXpgS-ALHEBpID_xE58QaoqoEgrcYGzNUkmuy6JvdjOin57vGxZluKLLc_P73nmEfKcs7ecyfZdirjlotXiETkXrFaVkEI8PjmfkcuUbllZSpiam6fkTGhRG870Ofm5Qpc2M3gH1PkR--yCp3MYlgkyJtphhoqXUsZ1dJ5CAXbwGwI_UJgyxkTzBul35zG7PtEwlvc-9HcZadpGhMH5NQ07jHR0XQx-b-KfkScjTAkv7_cL8u3jh6_XN9Xqy6fP11erqleyzZVhTGoAbAateWmGNXIYR9FrFHU9KK2wMx03ogNVhtA1bT22g5RGKCNRiVpekPcH3e3SzTj06HOEyW6jmyHe2QDO_lvxbmPXYWdrJVrBeBF4fRDYPHh2c7Wy-zvGuWkbbXZ79tW9WQw_FkzZzi71OE3gMSzJ8oYJXSZvWEFfPkBvwxJ9GYXlrTGNlMrov-Z9DKkkPR5_wJndx2-P8Rf2xWmnR_JP2AV4cwBKKiUSjCeW_6n9At_6ug0</recordid><startdate>20150807</startdate><enddate>20150807</enddate><creator>Figueira, Cláudio Pereira</creator><creator>Carvalhal, Djalma Gomes Ferrão</creator><creator>Almeida, Rafaela Andrade</creator><creator>Hermida, Micely d’ El-Rei</creator><creator>Touchard, Dominique</creator><creator>Robert, Phillipe</creator><creator>Pierres, Anne</creator><creator>Bongrand, Pierre</creator><creator>dos-Santos, Washington LC</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope></search><sort><creationdate>20150807</creationdate><title>Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin</title><author>Figueira, Cláudio Pereira ; Carvalhal, Djalma Gomes Ferrão ; Almeida, Rafaela Andrade ; Hermida, Micely d’ El-Rei ; Touchard, Dominique ; Robert, Phillipe ; Pierres, Anne ; Bongrand, Pierre ; dos-Santos, Washington LC</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-90036aae7d661322073dff2c6e244d565eb9b192ba5128b784f8d3392593e5243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>14</topic><topic>14/28</topic><topic>14/63</topic><topic>631/326/417/2546</topic><topic>631/80/79/1236</topic><topic>Affinity</topic><topic>Cell activation</topic><topic>Cell Adhesion - physiology</topic><topic>Cytoplasm</topic><topic>Epitopes</topic><topic>Fibronectin</topic><topic>Fibronectins - metabolism</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Integrin alpha4beta1 - metabolism</topic><topic>Integrin beta1 - metabolism</topic><topic>Kinetics</topic><topic>Leishmania - metabolism</topic><topic>Leishmaniasis</topic><topic>Leishmaniasis - metabolism</topic><topic>Leishmaniasis - parasitology</topic><topic>Leukocytes - metabolism</topic><topic>Leukocytes - parasitology</topic><topic>Life Sciences</topic><topic>Monocytes</topic><topic>Monocytes - metabolism</topic><topic>Monocytes - parasitology</topic><topic>multidisciplinary</topic><topic>Parasites</topic><topic>Science</topic><topic>Spreading</topic><topic>Vector-borne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Figueira, Cláudio Pereira</creatorcontrib><creatorcontrib>Carvalhal, Djalma Gomes Ferrão</creatorcontrib><creatorcontrib>Almeida, Rafaela Andrade</creatorcontrib><creatorcontrib>Hermida, Micely d’ El-Rei</creatorcontrib><creatorcontrib>Touchard, Dominique</creatorcontrib><creatorcontrib>Robert, Phillipe</creatorcontrib><creatorcontrib>Pierres, Anne</creatorcontrib><creatorcontrib>Bongrand, Pierre</creatorcontrib><creatorcontrib>dos-Santos, Washington LC</creatorcontrib><collection>Springer Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>ProQuest Biological Science Journals</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Figueira, Cláudio Pereira</au><au>Carvalhal, Djalma Gomes Ferrão</au><au>Almeida, Rafaela Andrade</au><au>Hermida, Micely d’ El-Rei</au><au>Touchard, Dominique</au><au>Robert, Phillipe</au><au>Pierres, Anne</au><au>Bongrand, Pierre</au><au>dos-Santos, Washington LC</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2015-08-07</date><risdate>2015</risdate><volume>5</volume><issue>1</issue><spage>12862</spage><epage>12862</epage><pages>12862-12862</pages><artnum>12862</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Contact with
Leishmania
leads to a decreases in mononuclear phagocyte adherence to connective tissue. In this work, we studied the early stages of bond formation between VLA4 and fibronectin, measured the kinetics of membrane alignment and the monocyte cytoplasm spreading area over a fibronectin-coated surface and studied the expression of high affinity integrin epitope in uninfected and
Leishmania
-infected human monocytes. Our results show that the initial VLA4-mediated interaction of
Leishmania
-infected monocyte with a fibronectin-coated surface is preserved, however, the later stage, leukocyte spreading over the substrate is abrogated in
Leishmania
-infected cells. The median of spreading area was 72 [55–89] μm
2
for uninfected and 41 [34–51] μm
2
for
Leishmania
-infected monocyte. This cytoplasm spread was inhibited using an anti-VLA4 blocking antibody. After the initial contact with the fibronectrin-coated surface, uninfected monocyte quickly spread the cytoplasm at a 15 μm
2
s
−1
ratio whilst
Leishmania
-infected monocytes only made small contacts at a 5.5 μm
2
s
−1
ratio. The expression of high affinity epitope by VLA4 (from 39 ± 21% to 14 ± 3%); and LFA1 (from 37 ± 32% to 18 ± 16%) molecules was reduced in
Leishmania
-infected monocytes. These changes in phagocyte function may be important for parasite dissemination and distribution of lesions in leishmaniasis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>26249106</pmid><doi>10.1038/srep12862</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 14 14/28 14/63 631/326/417/2546 631/80/79/1236 Affinity Cell activation Cell Adhesion - physiology Cytoplasm Epitopes Fibronectin Fibronectins - metabolism Humanities and Social Sciences Humans Integrin alpha4beta1 - metabolism Integrin beta1 - metabolism Kinetics Leishmania - metabolism Leishmaniasis Leishmaniasis - metabolism Leishmaniasis - parasitology Leukocytes - metabolism Leukocytes - parasitology Life Sciences Monocytes Monocytes - metabolism Monocytes - parasitology multidisciplinary Parasites Science Spreading Vector-borne diseases |
title | Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin |
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