Comet assay based DNA evaluation of fuel filling stations and automobile workshops workers from Khyber Pakhtunkhwa province, Pakistan
Fuel filling stations workers and automobile workshops mechanics are consistently exposed to gasoline hydrocarbons during their occupation, this may cause DNA damage. Objective of this study was to evaluate the level of DNA damage in subjects occupationally exposed to these hydrocarbons. Comet assay...
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| Veröffentlicht in: | Journal of occupational medicine and toxicology (London, England) England), 2015-08, Vol.10 (1), p.27-27, Article 27 |
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| container_title | Journal of occupational medicine and toxicology (London, England) |
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| creator | Khisroon, Muhammad Gul, Aisha Khan, Ajmal Ali, Naheed Zaidi, Farah Rasheed, Syed Basit Ahmadullah Akbar, Huma |
| description | Fuel filling stations workers and automobile workshops mechanics are consistently exposed to gasoline hydrocarbons during their occupation, this may cause DNA damage. Objective of this study was to evaluate the level of DNA damage in subjects occupationally exposed to these hydrocarbons.
Comet assay was performed on blood lymphocytes of exposed subjects to assess the probable DNA damage. 100 cells per individual were scored and graded by comet tail length. Exposed group consisted of 98 subjects (age 25.4 ± 7.2 years), of which 68 were CNG/Petrol filling men and 30 were automobile workshop workers, selected randomly from different service stations and automobile workshops of populated and adjacent cities of Peshawar, Mardan and Nowshera of Khyber Pakhtunkhwa province, while control group included 92 subjects (age 26.7 ± 11.8 years) were also from the same areas.
Significantly high level of DNA damage was found in the subjects exposed to gasoline hydrocarbons as compared to control subjects (173.2 ± 50.1 and 61.0 ± 25.0, P = 0.001, respectively). Period of exposure and use of tobacco also showed considerable effects (P 0.05).
The results of our study concluded that petroleum hydrocarbons have the potential to cause DNA damage in the exposed subjects. The study also suggested that protective strategies should be implemented by the concerned authorities to minimize exposure to fuel hydrocarbons. |
| doi_str_mv | 10.1186/s12995-015-0069-2 |
| format | Article |
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Comet assay was performed on blood lymphocytes of exposed subjects to assess the probable DNA damage. 100 cells per individual were scored and graded by comet tail length. Exposed group consisted of 98 subjects (age 25.4 ± 7.2 years), of which 68 were CNG/Petrol filling men and 30 were automobile workshop workers, selected randomly from different service stations and automobile workshops of populated and adjacent cities of Peshawar, Mardan and Nowshera of Khyber Pakhtunkhwa province, while control group included 92 subjects (age 26.7 ± 11.8 years) were also from the same areas.
Significantly high level of DNA damage was found in the subjects exposed to gasoline hydrocarbons as compared to control subjects (173.2 ± 50.1 and 61.0 ± 25.0, P = 0.001, respectively). Period of exposure and use of tobacco also showed considerable effects (P < 0.05) on DNA damage, while effect of age and daily working hours on total comet score (TCS) were non-significant (P > 0.05).
The results of our study concluded that petroleum hydrocarbons have the potential to cause DNA damage in the exposed subjects. The study also suggested that protective strategies should be implemented by the concerned authorities to minimize exposure to fuel hydrocarbons.</description><identifier>ISSN: 1745-6673</identifier><identifier>EISSN: 1745-6673</identifier><identifier>DOI: 10.1186/s12995-015-0069-2</identifier><identifier>PMID: 26236388</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Conferences, meetings and seminars ; DNA ; Gasoline ; Genetic research ; Health aspects ; Occupational health and safety ; Service stations (Motor vehicles) ; Workers ; Workshops (Educational programs)</subject><ispartof>Journal of occupational medicine and toxicology (London, England), 2015-08, Vol.10 (1), p.27-27, Article 27</ispartof><rights>COPYRIGHT 2015 BioMed Central Ltd.</rights><rights>Khisroon et al. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-9bda3d048d33c0777b49521655126445f4b9f152c8b042c47d1dfe3201e206d03</citedby><cites>FETCH-LOGICAL-c466t-9bda3d048d33c0777b49521655126445f4b9f152c8b042c47d1dfe3201e206d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522117/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4522117/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26236388$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khisroon, Muhammad</creatorcontrib><creatorcontrib>Gul, Aisha</creatorcontrib><creatorcontrib>Khan, Ajmal</creatorcontrib><creatorcontrib>Ali, Naheed</creatorcontrib><creatorcontrib>Zaidi, Farah</creatorcontrib><creatorcontrib>Rasheed, Syed Basit</creatorcontrib><creatorcontrib>Ahmadullah</creatorcontrib><creatorcontrib>Akbar, Huma</creatorcontrib><title>Comet assay based DNA evaluation of fuel filling stations and automobile workshops workers from Khyber Pakhtunkhwa province, Pakistan</title><title>Journal of occupational medicine and toxicology (London, England)</title><addtitle>J Occup Med Toxicol</addtitle><description>Fuel filling stations workers and automobile workshops mechanics are consistently exposed to gasoline hydrocarbons during their occupation, this may cause DNA damage. Objective of this study was to evaluate the level of DNA damage in subjects occupationally exposed to these hydrocarbons.
Comet assay was performed on blood lymphocytes of exposed subjects to assess the probable DNA damage. 100 cells per individual were scored and graded by comet tail length. Exposed group consisted of 98 subjects (age 25.4 ± 7.2 years), of which 68 were CNG/Petrol filling men and 30 were automobile workshop workers, selected randomly from different service stations and automobile workshops of populated and adjacent cities of Peshawar, Mardan and Nowshera of Khyber Pakhtunkhwa province, while control group included 92 subjects (age 26.7 ± 11.8 years) were also from the same areas.
Significantly high level of DNA damage was found in the subjects exposed to gasoline hydrocarbons as compared to control subjects (173.2 ± 50.1 and 61.0 ± 25.0, P = 0.001, respectively). Period of exposure and use of tobacco also showed considerable effects (P < 0.05) on DNA damage, while effect of age and daily working hours on total comet score (TCS) were non-significant (P > 0.05).
The results of our study concluded that petroleum hydrocarbons have the potential to cause DNA damage in the exposed subjects. The study also suggested that protective strategies should be implemented by the concerned authorities to minimize exposure to fuel hydrocarbons.</description><subject>Conferences, meetings and seminars</subject><subject>DNA</subject><subject>Gasoline</subject><subject>Genetic research</subject><subject>Health aspects</subject><subject>Occupational health and safety</subject><subject>Service stations (Motor vehicles)</subject><subject>Workers</subject><subject>Workshops (Educational programs)</subject><issn>1745-6673</issn><issn>1745-6673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNptks1u1DAUhSMEoqXwAGyQJTYsSPF_kg3SaKAFUQELWFtOfD0x49iDnUw1D8B7k3RK1UrIsnx1fc7RtfUVxUuCzwmp5btMaNOIEpN5Y9mU9FFxSiouSikr9vhefVI8y_kXxlwIzJ4WJ1RSJlldnxZ_1nGAEemc9QG1OoNBH76uEOy1n_ToYkDRIjuBR9Z578IG5fGmn5EOBulpjENsnQd0HdM293GXbypIGdkUB_SlP7SQ0He97ccpbPtrjXYp7l3o4O3SdXNeeF48sdpneHF7nhU_Lz7-WH8qr75dfl6vrsqOSzmWTWs0M5jXhrEOV1XV8kZQIoUgVHIuLG8bSwTt6hZz2vHKEGOBUUyAYmkwOyveH3N3UzuA6SCMSXu1S27Q6aCidurhTXC92sS94oJSQqo54M1tQIq_J8ijGlzuwHsdIE5ZkQoTRnDTsFn6-ijdaA_KBRvnxG6Rq5XgRDAqGZ1V5_9RzcvA4LoYYP52eGggR0OXYs4J7N30BKuFCnWkQs1UqIUKtXhe3X_2neMfBuwvycCz8A</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Khisroon, Muhammad</creator><creator>Gul, Aisha</creator><creator>Khan, Ajmal</creator><creator>Ali, Naheed</creator><creator>Zaidi, Farah</creator><creator>Rasheed, Syed Basit</creator><creator>Ahmadullah</creator><creator>Akbar, Huma</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150801</creationdate><title>Comet assay based DNA evaluation of fuel filling stations and automobile workshops workers from Khyber Pakhtunkhwa province, Pakistan</title><author>Khisroon, Muhammad ; Gul, Aisha ; Khan, Ajmal ; Ali, Naheed ; Zaidi, Farah ; Rasheed, Syed Basit ; Ahmadullah ; Akbar, Huma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-9bda3d048d33c0777b49521655126445f4b9f152c8b042c47d1dfe3201e206d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Conferences, meetings and seminars</topic><topic>DNA</topic><topic>Gasoline</topic><topic>Genetic research</topic><topic>Health aspects</topic><topic>Occupational health and safety</topic><topic>Service stations (Motor vehicles)</topic><topic>Workers</topic><topic>Workshops (Educational programs)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khisroon, Muhammad</creatorcontrib><creatorcontrib>Gul, Aisha</creatorcontrib><creatorcontrib>Khan, Ajmal</creatorcontrib><creatorcontrib>Ali, Naheed</creatorcontrib><creatorcontrib>Zaidi, Farah</creatorcontrib><creatorcontrib>Rasheed, Syed Basit</creatorcontrib><creatorcontrib>Ahmadullah</creatorcontrib><creatorcontrib>Akbar, Huma</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of occupational medicine and toxicology (London, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khisroon, Muhammad</au><au>Gul, Aisha</au><au>Khan, Ajmal</au><au>Ali, Naheed</au><au>Zaidi, Farah</au><au>Rasheed, Syed Basit</au><au>Ahmadullah</au><au>Akbar, Huma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comet assay based DNA evaluation of fuel filling stations and automobile workshops workers from Khyber Pakhtunkhwa province, Pakistan</atitle><jtitle>Journal of occupational medicine and toxicology (London, England)</jtitle><addtitle>J Occup Med Toxicol</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>10</volume><issue>1</issue><spage>27</spage><epage>27</epage><pages>27-27</pages><artnum>27</artnum><issn>1745-6673</issn><eissn>1745-6673</eissn><abstract>Fuel filling stations workers and automobile workshops mechanics are consistently exposed to gasoline hydrocarbons during their occupation, this may cause DNA damage. Objective of this study was to evaluate the level of DNA damage in subjects occupationally exposed to these hydrocarbons.
Comet assay was performed on blood lymphocytes of exposed subjects to assess the probable DNA damage. 100 cells per individual were scored and graded by comet tail length. Exposed group consisted of 98 subjects (age 25.4 ± 7.2 years), of which 68 were CNG/Petrol filling men and 30 were automobile workshop workers, selected randomly from different service stations and automobile workshops of populated and adjacent cities of Peshawar, Mardan and Nowshera of Khyber Pakhtunkhwa province, while control group included 92 subjects (age 26.7 ± 11.8 years) were also from the same areas.
Significantly high level of DNA damage was found in the subjects exposed to gasoline hydrocarbons as compared to control subjects (173.2 ± 50.1 and 61.0 ± 25.0, P = 0.001, respectively). Period of exposure and use of tobacco also showed considerable effects (P < 0.05) on DNA damage, while effect of age and daily working hours on total comet score (TCS) were non-significant (P > 0.05).
The results of our study concluded that petroleum hydrocarbons have the potential to cause DNA damage in the exposed subjects. The study also suggested that protective strategies should be implemented by the concerned authorities to minimize exposure to fuel hydrocarbons.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>26236388</pmid><doi>10.1186/s12995-015-0069-2</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; SpringerLink Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access; Springer Nature OA Free Journals |
| subjects | Conferences, meetings and seminars DNA Gasoline Genetic research Health aspects Occupational health and safety Service stations (Motor vehicles) Workers Workshops (Educational programs) |
| title | Comet assay based DNA evaluation of fuel filling stations and automobile workshops workers from Khyber Pakhtunkhwa province, Pakistan |
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