Potential Effect of CD271 on Human Mesenchymal Stromal Cell Proliferation and Differentiation
The Low-Affinity Nerve Growth Factor Receptor (LNGFR), also known as CD271, is a member of the tumor necrosis factor receptor superfamily. The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow a...
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| Veröffentlicht in: | International journal of molecular sciences 2015-07, Vol.16 (7), p.15609-15624 |
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| creator | Calabrese, Giovanna Giuffrida, Raffaella Lo Furno, Debora Parrinello, Nunziatina Laura Forte, Stefano Gulino, Rosario Colarossi, Cristina Schinocca, Luciana Rita Giuffrida, Rosario Cardile, Venera Memeo, Lorenzo |
| description | The Low-Affinity Nerve Growth Factor Receptor (LNGFR), also known as CD271, is a member of the tumor necrosis factor receptor superfamily. The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow aspirate. In this study, we compare the proliferative and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells. Mesenchymal stromal cells were isolated from bone marrow aspirate and adipose tissue by plastic adherence and positive selection. The proliferation and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells were assessed by inducing osteogenic, adipogenic and chondrogenic in vitro differentiation. Compared to CD271+, CD271- mesenchymal stromal cells showed a lower proliferation rate and a decreased ability to give rise to osteocytes, adipocytes and chondrocytes. Furthermore, we observed that CD271+ mesenchymal stromal cells isolated from adipose tissue displayed a higher efficiency of proliferation and trilineage differentiation compared to CD271+ mesenchymal stromal cells isolated from bone marrow samples, although the CD271 expression levels were comparable. In conclusion, these data show that both the presence of CD271 antigen and the source of mesenchymal stromal cells represent important factors in determining the ability of the cells to proliferate and differentiate. |
| doi_str_mv | 10.3390/ijms160715609 |
| format | Article |
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The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow aspirate. In this study, we compare the proliferative and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells. Mesenchymal stromal cells were isolated from bone marrow aspirate and adipose tissue by plastic adherence and positive selection. The proliferation and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells were assessed by inducing osteogenic, adipogenic and chondrogenic in vitro differentiation. Compared to CD271+, CD271- mesenchymal stromal cells showed a lower proliferation rate and a decreased ability to give rise to osteocytes, adipocytes and chondrocytes. Furthermore, we observed that CD271+ mesenchymal stromal cells isolated from adipose tissue displayed a higher efficiency of proliferation and trilineage differentiation compared to CD271+ mesenchymal stromal cells isolated from bone marrow samples, although the CD271 expression levels were comparable. In conclusion, these data show that both the presence of CD271 antigen and the source of mesenchymal stromal cells represent important factors in determining the ability of the cells to proliferate and differentiate.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms160715609</identifier><identifier>PMID: 26184166</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adipogenesis ; Aged ; Bone marrow ; Bone Marrow Cells - cytology ; Cartilage ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Cellular biology ; Chondrogenesis ; Female ; Humans ; Immunohistochemistry ; Male ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Middle Aged ; Nerve Tissue Proteins - metabolism ; Osteogenesis ; Phenotype ; Receptors, Nerve Growth Factor - metabolism</subject><ispartof>International journal of molecular sciences, 2015-07, Vol.16 (7), p.15609-15624</ispartof><rights>Copyright MDPI AG 2015</rights><rights>2015 by the authors; licensee MDPI, Basel, Switzerland. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-b139e34254dfa41de9d02c0fa959bb10e15f34db465232595aff2467e92a93de3</citedby><cites>FETCH-LOGICAL-c514t-b139e34254dfa41de9d02c0fa959bb10e15f34db465232595aff2467e92a93de3</cites><orcidid>0000-0001-5746-7451 ; 0000-0001-7494-3675 ; 0000-0003-4251-7203 ; 0000-0001-5395-8608</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519916/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4519916/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26184166$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Calabrese, Giovanna</creatorcontrib><creatorcontrib>Giuffrida, Raffaella</creatorcontrib><creatorcontrib>Lo Furno, Debora</creatorcontrib><creatorcontrib>Parrinello, Nunziatina Laura</creatorcontrib><creatorcontrib>Forte, Stefano</creatorcontrib><creatorcontrib>Gulino, Rosario</creatorcontrib><creatorcontrib>Colarossi, Cristina</creatorcontrib><creatorcontrib>Schinocca, Luciana Rita</creatorcontrib><creatorcontrib>Giuffrida, Rosario</creatorcontrib><creatorcontrib>Cardile, Venera</creatorcontrib><creatorcontrib>Memeo, Lorenzo</creatorcontrib><title>Potential Effect of CD271 on Human Mesenchymal Stromal Cell Proliferation and Differentiation</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>The Low-Affinity Nerve Growth Factor Receptor (LNGFR), also known as CD271, is a member of the tumor necrosis factor receptor superfamily. The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow aspirate. In this study, we compare the proliferative and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells. Mesenchymal stromal cells were isolated from bone marrow aspirate and adipose tissue by plastic adherence and positive selection. The proliferation and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells were assessed by inducing osteogenic, adipogenic and chondrogenic in vitro differentiation. Compared to CD271+, CD271- mesenchymal stromal cells showed a lower proliferation rate and a decreased ability to give rise to osteocytes, adipocytes and chondrocytes. Furthermore, we observed that CD271+ mesenchymal stromal cells isolated from adipose tissue displayed a higher efficiency of proliferation and trilineage differentiation compared to CD271+ mesenchymal stromal cells isolated from bone marrow samples, although the CD271 expression levels were comparable. In conclusion, these data show that both the presence of CD271 antigen and the source of mesenchymal stromal cells represent important factors in determining the ability of the cells to proliferate and differentiate.</description><subject>Adipogenesis</subject><subject>Aged</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>Cartilage</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Chondrogenesis</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Middle Aged</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Osteogenesis</subject><subject>Phenotype</subject><subject>Receptors, Nerve Growth Factor - metabolism</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkUtLxDAUhYMovpdupeDGTTU3r042goxPUBxQlxLSNnEytI0mreC_NzM-GN24uiH343DOPQjtAT6iVOJjN2sjCFwAF1iuoE1ghOQYi2J16b2BtmKcYUwo4XIdbRABIwZCbKKnie9N1zvdZOfWmqrPvM3GZ6SAzHfZ1dDqLrs10XTV9L1N0H0f_HyOTdNkk-AbZ03QvUuw7urszCWRsBCc_-2gNaubaHa_5jZ6vDh_GF_lN3eX1-PTm7ziwPq8BCoNZYSz2moGtZE1JhW2WnJZloANcEtZXTLBFwm4tpYwURhJtKS1odvo5FP3ZShbU1fJQNCNegmu1eFdee3U703npurZvynGQUoQSeDwSyD418HEXrUuVimj7owfooIRLWhyyOB_VMhROjQQmtCDP-jMD6FLl1BQYEZFkRQTlX9SVfAxBmN_fANW847Vr44Tv78c9of-LpV-AJtWods</recordid><startdate>20150709</startdate><enddate>20150709</enddate><creator>Calabrese, Giovanna</creator><creator>Giuffrida, Raffaella</creator><creator>Lo Furno, Debora</creator><creator>Parrinello, Nunziatina Laura</creator><creator>Forte, Stefano</creator><creator>Gulino, Rosario</creator><creator>Colarossi, Cristina</creator><creator>Schinocca, Luciana Rita</creator><creator>Giuffrida, Rosario</creator><creator>Cardile, Venera</creator><creator>Memeo, Lorenzo</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7TK</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5746-7451</orcidid><orcidid>https://orcid.org/0000-0001-7494-3675</orcidid><orcidid>https://orcid.org/0000-0003-4251-7203</orcidid><orcidid>https://orcid.org/0000-0001-5395-8608</orcidid></search><sort><creationdate>20150709</creationdate><title>Potential Effect of CD271 on Human Mesenchymal Stromal Cell Proliferation and Differentiation</title><author>Calabrese, Giovanna ; 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The CD271 cell surface marker defines a subset of multipotential mesenchymal stromal cells and may be used to isolate and enrich cells derived from bone marrow aspirate. In this study, we compare the proliferative and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells. Mesenchymal stromal cells were isolated from bone marrow aspirate and adipose tissue by plastic adherence and positive selection. The proliferation and differentiation potentials of CD271+ and CD271- mesenchymal stromal cells were assessed by inducing osteogenic, adipogenic and chondrogenic in vitro differentiation. Compared to CD271+, CD271- mesenchymal stromal cells showed a lower proliferation rate and a decreased ability to give rise to osteocytes, adipocytes and chondrocytes. Furthermore, we observed that CD271+ mesenchymal stromal cells isolated from adipose tissue displayed a higher efficiency of proliferation and trilineage differentiation compared to CD271+ mesenchymal stromal cells isolated from bone marrow samples, although the CD271 expression levels were comparable. In conclusion, these data show that both the presence of CD271 antigen and the source of mesenchymal stromal cells represent important factors in determining the ability of the cells to proliferate and differentiate.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>26184166</pmid><doi>10.3390/ijms160715609</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0001-5746-7451</orcidid><orcidid>https://orcid.org/0000-0001-7494-3675</orcidid><orcidid>https://orcid.org/0000-0003-4251-7203</orcidid><orcidid>https://orcid.org/0000-0001-5395-8608</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adipogenesis Aged Bone marrow Bone Marrow Cells - cytology Cartilage Cell Differentiation Cell Proliferation Cells, Cultured Cellular biology Chondrogenesis Female Humans Immunohistochemistry Male Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Middle Aged Nerve Tissue Proteins - metabolism Osteogenesis Phenotype Receptors, Nerve Growth Factor - metabolism |
| title | Potential Effect of CD271 on Human Mesenchymal Stromal Cell Proliferation and Differentiation |
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