Measuring T cell immunity to influenza vaccination in children after haemopoietic stem cell transplantation
Summary Quantitative assessment of immunogen‐specific T cell responses may provide a meaningful surrogate marker of functional immunity in patients following haemopoietic stem cell transplantation (HSCT). We developed a flow‐cytometric assay to quantify antigen‐specific T cell immunity to influenza‐...
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| Veröffentlicht in: | British journal of haematology 2004-11, Vol.127 (3), p.322-325 |
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| container_title | British journal of haematology |
| container_volume | 127 |
| creator | Haining, W. Nicholas Evans, John W. Seth, Nilufer P. Callaway, G. Derek Wucherpfennig, Kai W. Nadler, Lee M. Guinan, Eva C. |
| description | Summary
Quantitative assessment of immunogen‐specific T cell responses may provide a meaningful surrogate marker of functional immunity in patients following haemopoietic stem cell transplantation (HSCT). We developed a flow‐cytometric assay to quantify antigen‐specific T cell immunity to influenza‐A and studied the T cell response to influenza vaccination in five children, 3–21 months post‐HSCT. All patients showed an increase in influenza‐A‐specific CD4+ immunity following vaccination while none had a detectable IgG response to the vaccine. This assay proved sufficiently sensitive to evaluate changes in T cell memory in immunocompromised individuals and could be used to better characterize post‐HSCT immune reconstitution. |
| doi_str_mv | 10.1111/j.1365-2141.2004.05204.x |
| format | Article |
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Quantitative assessment of immunogen‐specific T cell responses may provide a meaningful surrogate marker of functional immunity in patients following haemopoietic stem cell transplantation (HSCT). We developed a flow‐cytometric assay to quantify antigen‐specific T cell immunity to influenza‐A and studied the T cell response to influenza vaccination in five children, 3–21 months post‐HSCT. All patients showed an increase in influenza‐A‐specific CD4+ immunity following vaccination while none had a detectable IgG response to the vaccine. This assay proved sufficiently sensitive to evaluate changes in T cell memory in immunocompromised individuals and could be used to better characterize post‐HSCT immune reconstitution.</description><identifier>ISSN: 0007-1048</identifier><identifier>EISSN: 1365-2141</identifier><identifier>DOI: 10.1111/j.1365-2141.2004.05204.x</identifier><identifier>PMID: 15491293</identifier><identifier>CODEN: BJHEAL</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Adolescent ; Biological and medical sciences ; BMT ; CD4 Antigens - analysis ; Child ; Child, Preschool ; clinical studies ; Flow Cytometry ; Hematologic and hematopoietic diseases ; Hematology ; Hematopoietic Stem Cell Transplantation ; Humans ; Immunocompromised Host ; Immunologic Memory ; Influenza A virus - immunology ; Influenza Vaccines - administration & dosage ; Medical sciences ; Statistics, Nonparametric ; T cells ; T-Lymphocytes - immunology ; vaccines</subject><ispartof>British journal of haematology, 2004-11, Vol.127 (3), p.322-325</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Nov 2004</rights><rights>2004 Blackwell Publishing Ltd 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5274-43fa08345c73199ca29d2654409f86a504a3b70915e408b45030954bf62334283</citedby><cites>FETCH-LOGICAL-c5274-43fa08345c73199ca29d2654409f86a504a3b70915e408b45030954bf62334283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2141.2004.05204.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2141.2004.05204.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16195774$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15491293$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haining, W. Nicholas</creatorcontrib><creatorcontrib>Evans, John W.</creatorcontrib><creatorcontrib>Seth, Nilufer P.</creatorcontrib><creatorcontrib>Callaway, G. Derek</creatorcontrib><creatorcontrib>Wucherpfennig, Kai W.</creatorcontrib><creatorcontrib>Nadler, Lee M.</creatorcontrib><creatorcontrib>Guinan, Eva C.</creatorcontrib><title>Measuring T cell immunity to influenza vaccination in children after haemopoietic stem cell transplantation</title><title>British journal of haematology</title><addtitle>Br J Haematol</addtitle><description>Summary
Quantitative assessment of immunogen‐specific T cell responses may provide a meaningful surrogate marker of functional immunity in patients following haemopoietic stem cell transplantation (HSCT). We developed a flow‐cytometric assay to quantify antigen‐specific T cell immunity to influenza‐A and studied the T cell response to influenza vaccination in five children, 3–21 months post‐HSCT. All patients showed an increase in influenza‐A‐specific CD4+ immunity following vaccination while none had a detectable IgG response to the vaccine. This assay proved sufficiently sensitive to evaluate changes in T cell memory in immunocompromised individuals and could be used to better characterize post‐HSCT immune reconstitution.</description><subject>Adolescent</subject><subject>Biological and medical sciences</subject><subject>BMT</subject><subject>CD4 Antigens - analysis</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>clinical studies</subject><subject>Flow Cytometry</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Hematology</subject><subject>Hematopoietic Stem Cell Transplantation</subject><subject>Humans</subject><subject>Immunocompromised Host</subject><subject>Immunologic Memory</subject><subject>Influenza A virus - immunology</subject><subject>Influenza Vaccines - administration & dosage</subject><subject>Medical sciences</subject><subject>Statistics, Nonparametric</subject><subject>T cells</subject><subject>T-Lymphocytes - immunology</subject><subject>vaccines</subject><issn>0007-1048</issn><issn>1365-2141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkVFvFCEUhYnR2HXtXzDERN9mvQwwAw-a1Ebbmhpf2mfCskyXdQZWYGrXXy_T3bTqkzwA4X735FwOQpjAgpT1brMgtOFVTRhZ1ABsAbwu-90TNHsoPEUzAGgrAkwcoRcpbQAIBU6eoyPCmSS1pDP0_avVaYzO3-ArbGzfYzcMo3d5h3PAznf9aP0vjW-1Mc7r7IIvr9isXb-K1mPdZRvxWtshbIOz2Rmcsh32Ujlqn7a99vm-8SV61uk-2ePDOUfXnz9dnZ5Xl9_OLk5PLivD65ZVjHYaBGXctJRIaXQtV3XDGQPZiUZzYJouW5CEWwZiyThQkJwtu6amlNWCztGHve52XA52ZawvRnq1jW7QcaeCdurvindrdRNuFeOkgSIyR28PAjH8GG3KanBpmkh7G8akmkYKTjkv4Ot_wE0Yoy_DKSJFw0EIViCxh0wMKUXbPTghoKY41UZNqakpNTXFqe7jVHel9dWfkzw2HvIrwJsDoJPRfVc-3Lj0yDVE8radPLzfcz9db3f_bUB9_HI-3ehvH3S7jA</recordid><startdate>200411</startdate><enddate>200411</enddate><creator>Haining, W. Nicholas</creator><creator>Evans, John W.</creator><creator>Seth, Nilufer P.</creator><creator>Callaway, G. Derek</creator><creator>Wucherpfennig, Kai W.</creator><creator>Nadler, Lee M.</creator><creator>Guinan, Eva C.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Blackwell Publishing Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200411</creationdate><title>Measuring T cell immunity to influenza vaccination in children after haemopoietic stem cell transplantation</title><author>Haining, W. Nicholas ; Evans, John W. ; Seth, Nilufer P. ; Callaway, G. Derek ; Wucherpfennig, Kai W. ; Nadler, Lee M. ; Guinan, Eva C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5274-43fa08345c73199ca29d2654409f86a504a3b70915e408b45030954bf62334283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adolescent</topic><topic>Biological and medical sciences</topic><topic>BMT</topic><topic>CD4 Antigens - analysis</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>clinical studies</topic><topic>Flow Cytometry</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Hematology</topic><topic>Hematopoietic Stem Cell Transplantation</topic><topic>Humans</topic><topic>Immunocompromised Host</topic><topic>Immunologic Memory</topic><topic>Influenza A virus - immunology</topic><topic>Influenza Vaccines - administration & dosage</topic><topic>Medical sciences</topic><topic>Statistics, Nonparametric</topic><topic>T cells</topic><topic>T-Lymphocytes - immunology</topic><topic>vaccines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haining, W. Nicholas</creatorcontrib><creatorcontrib>Evans, John W.</creatorcontrib><creatorcontrib>Seth, Nilufer P.</creatorcontrib><creatorcontrib>Callaway, G. Derek</creatorcontrib><creatorcontrib>Wucherpfennig, Kai W.</creatorcontrib><creatorcontrib>Nadler, Lee M.</creatorcontrib><creatorcontrib>Guinan, Eva C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of haematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haining, W. Nicholas</au><au>Evans, John W.</au><au>Seth, Nilufer P.</au><au>Callaway, G. Derek</au><au>Wucherpfennig, Kai W.</au><au>Nadler, Lee M.</au><au>Guinan, Eva C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measuring T cell immunity to influenza vaccination in children after haemopoietic stem cell transplantation</atitle><jtitle>British journal of haematology</jtitle><addtitle>Br J Haematol</addtitle><date>2004-11</date><risdate>2004</risdate><volume>127</volume><issue>3</issue><spage>322</spage><epage>325</epage><pages>322-325</pages><issn>0007-1048</issn><eissn>1365-2141</eissn><coden>BJHEAL</coden><abstract>Summary
Quantitative assessment of immunogen‐specific T cell responses may provide a meaningful surrogate marker of functional immunity in patients following haemopoietic stem cell transplantation (HSCT). We developed a flow‐cytometric assay to quantify antigen‐specific T cell immunity to influenza‐A and studied the T cell response to influenza vaccination in five children, 3–21 months post‐HSCT. All patients showed an increase in influenza‐A‐specific CD4+ immunity following vaccination while none had a detectable IgG response to the vaccine. This assay proved sufficiently sensitive to evaluate changes in T cell memory in immunocompromised individuals and could be used to better characterize post‐HSCT immune reconstitution.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15491293</pmid><doi>10.1111/j.1365-2141.2004.05204.x</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adolescent Biological and medical sciences BMT CD4 Antigens - analysis Child Child, Preschool clinical studies Flow Cytometry Hematologic and hematopoietic diseases Hematology Hematopoietic Stem Cell Transplantation Humans Immunocompromised Host Immunologic Memory Influenza A virus - immunology Influenza Vaccines - administration & dosage Medical sciences Statistics, Nonparametric T cells T-Lymphocytes - immunology vaccines |
| title | Measuring T cell immunity to influenza vaccination in children after haemopoietic stem cell transplantation |
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