Genistein-induced mir-23b expression inhibits the growth of breast cancer cells
Genistein, an isoflavonoid, plays roles in the inhibition of protein tyrosine kinase phosphorylation, induction of apoptosis, and cell differentiation in breast cancer. This study aims to induce cellular stress by exposing genistein to determine alterations of miRNA expression profiles in MCF-7 cell...
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| Veröffentlicht in: | Contemporary oncology (Poznan, Poland) Poland), 2015-01, Vol.19 (1), p.32-35 |
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| container_title | Contemporary oncology (Poznan, Poland) |
| container_volume | 19 |
| creator | Avci, Cigir Biray Susluer, Sunde Yilmaz Caglar, Hasan Onur Balci, Tugce Aygunes, Duygu Dodurga, Yavuz Gunduz, Cumhur |
| description | Genistein, an isoflavonoid, plays roles in the inhibition of protein tyrosine kinase phosphorylation, induction of apoptosis, and cell differentiation in breast cancer. This study aims to induce cellular stress by exposing genistein to determine alterations of miRNA expression profiles in MCF-7 cells.
XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment. Expressions of miRNAs were quantified using Real-Time Online RT-PCR.
The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein. It was found that miR-23b was upregulated for MCF-7 breast cancer cells after genistein treatment.
Up-regulated ex-expression of miR-23b might be a putative biomarker for use in the therapy of breast cancer patients. miR-23b up-regulation might be important in terms of response to genistein. |
| doi_str_mv | 10.5114/wo.2014.44121 |
| format | Article |
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XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment. Expressions of miRNAs were quantified using Real-Time Online RT-PCR.
The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein. It was found that miR-23b was upregulated for MCF-7 breast cancer cells after genistein treatment.
Up-regulated ex-expression of miR-23b might be a putative biomarker for use in the therapy of breast cancer patients. miR-23b up-regulation might be important in terms of response to genistein.</description><identifier>ISSN: 1428-2526</identifier><identifier>EISSN: 1897-4309</identifier><identifier>DOI: 10.5114/wo.2014.44121</identifier><identifier>PMID: 26199568</identifier><language>eng</language><publisher>Poland: Termedia Publishing House</publisher><subject>Breast cancer ; Original Paper</subject><ispartof>Contemporary oncology (Poznan, Poland), 2015-01, Vol.19 (1), p.32-35</ispartof><rights>Copyright Termedia Publishing House 2015</rights><rights>Copyright © 2015 Termedia 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-ee5c42f361292b31726695952c703612f30ad78bb4d6a579d0a89a1526a900783</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507883/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507883/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26199568$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Avci, Cigir Biray</creatorcontrib><creatorcontrib>Susluer, Sunde Yilmaz</creatorcontrib><creatorcontrib>Caglar, Hasan Onur</creatorcontrib><creatorcontrib>Balci, Tugce</creatorcontrib><creatorcontrib>Aygunes, Duygu</creatorcontrib><creatorcontrib>Dodurga, Yavuz</creatorcontrib><creatorcontrib>Gunduz, Cumhur</creatorcontrib><title>Genistein-induced mir-23b expression inhibits the growth of breast cancer cells</title><title>Contemporary oncology (Poznan, Poland)</title><addtitle>Contemp Oncol (Pozn)</addtitle><description>Genistein, an isoflavonoid, plays roles in the inhibition of protein tyrosine kinase phosphorylation, induction of apoptosis, and cell differentiation in breast cancer. This study aims to induce cellular stress by exposing genistein to determine alterations of miRNA expression profiles in MCF-7 cells.
XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment. Expressions of miRNAs were quantified using Real-Time Online RT-PCR.
The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein. It was found that miR-23b was upregulated for MCF-7 breast cancer cells after genistein treatment.
Up-regulated ex-expression of miR-23b might be a putative biomarker for use in the therapy of breast cancer patients. miR-23b up-regulation might be important in terms of response to genistein.</description><subject>Breast cancer</subject><subject>Original Paper</subject><issn>1428-2526</issn><issn>1897-4309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpVUU1LAzEQDaLYUnv0KgHPW_O9yUWQolUo9KLnkM1mu5E2qcnW6r93a7XoaYaZN2_ezAPgEqMJx5jd7OKEIMwmjGGCT8AQS1UWjCJ12ueMyIJwIgZgnLOvEMKl5ILSczAgAivFhRyCxcwFnzvnQ-FDvbWuhmufCkIr6D42yfWTMUAfWl_5LsOudXCZ4q5rYWxglZzJHbQmWJegdatVvgBnjVllN_6JI_DycP88fSzmi9nT9G5eWIZ5VzjHLSMNFZgoUlFcEiEUV5zYEu2LDUWmLmVVsVoYXqoaGakM7q8xCqFS0hG4PfButtXa1daFLpmV3iS_NulTR-P1_07wrV7Gd814Py5pT3D9Q5Di29blTr_GbQq9Zo1FSShXkrAeVRxQNsWck2uOGzDSewv0Luq9Bfrbgh5_9VfWEf37cPoFtKqBNQ</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Avci, Cigir Biray</creator><creator>Susluer, Sunde Yilmaz</creator><creator>Caglar, Hasan Onur</creator><creator>Balci, Tugce</creator><creator>Aygunes, Duygu</creator><creator>Dodurga, Yavuz</creator><creator>Gunduz, Cumhur</creator><general>Termedia Publishing House</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>20150101</creationdate><title>Genistein-induced mir-23b expression inhibits the growth of breast cancer cells</title><author>Avci, Cigir Biray ; 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This study aims to induce cellular stress by exposing genistein to determine alterations of miRNA expression profiles in MCF-7 cells.
XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment. Expressions of miRNAs were quantified using Real-Time Online RT-PCR.
The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein. It was found that miR-23b was upregulated for MCF-7 breast cancer cells after genistein treatment.
Up-regulated ex-expression of miR-23b might be a putative biomarker for use in the therapy of breast cancer patients. miR-23b up-regulation might be important in terms of response to genistein.</abstract><cop>Poland</cop><pub>Termedia Publishing House</pub><pmid>26199568</pmid><doi>10.5114/wo.2014.44121</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Breast cancer Original Paper |
| title | Genistein-induced mir-23b expression inhibits the growth of breast cancer cells |
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