Homologues of Genetic Transformation DNA Import Genes Are Required for Rhodobacter capsulatus Gene Transfer Agent Recipient Capability Regulated by the Response Regulator CtrA
Gene transfer agents (GTAs) morphologically resemble small, double-stranded DNA (dsDNA) bacteriophages; however, their only known role is to package and transfer random pieces of the producing cell genome to recipient cells. The best understood GTA is that of Rhodobacter capsulatus, termed RcGTA. We...
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| Veröffentlicht in: | Journal of bacteriology 2015-08, Vol.197 (16), p.2653-2663 |
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| creator | Brimacombe, Cedric A Ding, Hao Johnson, Jeanette A Beatty, J Thomas |
| description | Gene transfer agents (GTAs) morphologically resemble small, double-stranded DNA (dsDNA) bacteriophages; however, their only known role is to package and transfer random pieces of the producing cell genome to recipient cells. The best understood GTA is that of Rhodobacter capsulatus, termed RcGTA. We discovered that homologues of three genes involved in natural transformation in other bacteria, comEC, comF, and comM, are essential for RcGTA-mediated gene acquisition. This paper gives genetic and biochemical evidence that RcGTA-borne DNA entry into cells requires the ComEC and ComF putative DNA transport proteins and genetic evidence that putative cytoplasmic ComM protein of unknown function is required for recipient capability. Furthermore, the master regulator of RcGTA production in |
| doi_str_mv | 10.1128/JB.00332-15 |
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Horizontal gene transfer (HGT) is a major driver of bacterial evolution and adaptation to environmental stresses. Traits such as antibiotic resistance or metabolic properties can be transferred between bacteria via HGT; thus, HGT can have a tremendous effect on the fitness of a bacterial population. The three classically described HGT mechanisms are conjugation, transformation, and phage-mediated transduction. More recently, the HGT factor GTA was described, where random pieces of producing cell genome are packaged into phage-like particles that deliver DNA to recipient cells. In this report, we show that transport of DNA borne by the R. capsulatus RcGTA into recipient cells requires key genes previously thought to be specific to natural transformation pathways. These findings indicate that RcGTA combines central aspects of phage-mediated transduction and natural transformation in an efficient, regulated mode of HGT.</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>DOI: 10.1128/JB.00332-15</identifier><identifier>PMID: 26031909</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Bacterial proteins ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; Bacteriophages - genetics ; Computational Biology ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - genetics ; Gene Expression Regulation, Bacterial ; Gene Transfer, Horizontal ; Genetics ; Gram-negative bacteria ; Plasmids - genetics ; Recombinant Proteins - genetics ; Rhodobacter capsulatus ; Rhodobacter capsulatus - genetics ; Spotlight</subject><ispartof>Journal of bacteriology, 2015-08, Vol.197 (16), p.2653-2663</ispartof><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved.</rights><rights>Copyright American Society for Microbiology Aug 2015</rights><rights>Copyright © 2015, American Society for Microbiology. All Rights Reserved. 2015 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-b8482e83924f4112bd4c8a52d542f938327d2dc30323724c64a1c6b4daa1dce93</citedby><cites>FETCH-LOGICAL-c442t-b8482e83924f4112bd4c8a52d542f938327d2dc30323724c64a1c6b4daa1dce93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507343/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507343/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26031909$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brimacombe, Cedric A</creatorcontrib><creatorcontrib>Ding, Hao</creatorcontrib><creatorcontrib>Johnson, Jeanette A</creatorcontrib><creatorcontrib>Beatty, J Thomas</creatorcontrib><title>Homologues of Genetic Transformation DNA Import Genes Are Required for Rhodobacter capsulatus Gene Transfer Agent Recipient Capability Regulated by the Response Regulator CtrA</title><title>Journal of bacteriology</title><addtitle>J Bacteriol</addtitle><description>Gene transfer agents (GTAs) morphologically resemble small, double-stranded DNA (dsDNA) bacteriophages; however, their only known role is to package and transfer random pieces of the producing cell genome to recipient cells. The best understood GTA is that of Rhodobacter capsulatus, termed RcGTA. We discovered that homologues of three genes involved in natural transformation in other bacteria, comEC, comF, and comM, are essential for RcGTA-mediated gene acquisition. This paper gives genetic and biochemical evidence that RcGTA-borne DNA entry into cells requires the ComEC and ComF putative DNA transport proteins and genetic evidence that putative cytoplasmic ComM protein of unknown function is required for recipient capability. Furthermore, the master regulator of RcGTA production in <1% of a cell population, CtrA, which is also required for gene acquisition in recipient cells, is expressed in the vast majority of the population. Our results indicate that RcGTA-mediated gene transfer combines key aspects of two bacterial horizontal gene transfer mechanisms, where donor DNA is packaged in transducing phage-like particles and recipient cells take up DNA using natural transformation-related machinery. Both of these differentiated subsets of a culture population, donors and recipients, are dependent on the same response regulator, CtrA.
Horizontal gene transfer (HGT) is a major driver of bacterial evolution and adaptation to environmental stresses. Traits such as antibiotic resistance or metabolic properties can be transferred between bacteria via HGT; thus, HGT can have a tremendous effect on the fitness of a bacterial population. The three classically described HGT mechanisms are conjugation, transformation, and phage-mediated transduction. More recently, the HGT factor GTA was described, where random pieces of producing cell genome are packaged into phage-like particles that deliver DNA to recipient cells. In this report, we show that transport of DNA borne by the R. capsulatus RcGTA into recipient cells requires key genes previously thought to be specific to natural transformation pathways. These findings indicate that RcGTA combines central aspects of phage-mediated transduction and natural transformation in an efficient, regulated mode of HGT.</description><subject>Bacterial proteins</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Bacteriophages - genetics</subject><subject>Computational Biology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Gene Transfer, Horizontal</subject><subject>Genetics</subject><subject>Gram-negative bacteria</subject><subject>Plasmids - genetics</subject><subject>Recombinant Proteins - genetics</subject><subject>Rhodobacter capsulatus</subject><subject>Rhodobacter capsulatus - genetics</subject><subject>Spotlight</subject><issn>0021-9193</issn><issn>1098-5530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkktv1DAUhS0EokNhxR5ZYlMJpfiVxN4gpQP0oQqkqqwtx3FmXCV2ajtI86v4izjTaQVsWNm657vn2lcHgLcYnWJM-Mers1OEKCUFLp-BFUaCF2VJ0XOwQojgQmBBj8CrGO8QwoyV5CU4IhWiWCCxAr8u_OgHv5lNhL6H58aZZDW8DcrF3odRJesd_PytgZfj5EPaExE2wcAbcz_bYDqYOXiz9Z1vlU4mQK2mOA8qzXFPH8yy0GyMS7lP28kut7WaVGsHm3a5uFlaslu7g2m7uMfJu2gelTxjnULzGrzo1RDNm8N5DH58_XK7viiuv59frpvrQjNGUtFyxonhVBDWs7yltmOaq5J0JSO9oJySuiOdpogSWhOmK6awrlrWKYU7bQQ9Bp8efKe5HU0uuRTUIKdgRxV20isr_1ac3cqN_ylZiWrKaDY4ORgEf5-3m-RoozbDoJzxc5S4RgJXjNXk_2glakIwr1hG3_-D3vk5uLyJPVXzBczUhwdKBx9jMP3TuzGSS2bk1ZncZ0biMtPv_vzqE_sYEvobgDq-XA</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Brimacombe, Cedric A</creator><creator>Ding, Hao</creator><creator>Johnson, Jeanette A</creator><creator>Beatty, J Thomas</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150801</creationdate><title>Homologues of Genetic Transformation DNA Import Genes Are Required for Rhodobacter capsulatus Gene Transfer Agent Recipient Capability Regulated by the Response Regulator CtrA</title><author>Brimacombe, Cedric A ; Ding, Hao ; Johnson, Jeanette A ; Beatty, J Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-b8482e83924f4112bd4c8a52d542f938327d2dc30323724c64a1c6b4daa1dce93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bacterial proteins</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Bacteriophages - genetics</topic><topic>Computational Biology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Gene Transfer, Horizontal</topic><topic>Genetics</topic><topic>Gram-negative bacteria</topic><topic>Plasmids - genetics</topic><topic>Recombinant Proteins - genetics</topic><topic>Rhodobacter capsulatus</topic><topic>Rhodobacter capsulatus - genetics</topic><topic>Spotlight</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brimacombe, Cedric A</creatorcontrib><creatorcontrib>Ding, Hao</creatorcontrib><creatorcontrib>Johnson, Jeanette A</creatorcontrib><creatorcontrib>Beatty, J Thomas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brimacombe, Cedric A</au><au>Ding, Hao</au><au>Johnson, Jeanette A</au><au>Beatty, J Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Homologues of Genetic Transformation DNA Import Genes Are Required for Rhodobacter capsulatus Gene Transfer Agent Recipient Capability Regulated by the Response Regulator CtrA</atitle><jtitle>Journal of bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>197</volume><issue>16</issue><spage>2653</spage><epage>2663</epage><pages>2653-2663</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><coden>JOBAAY</coden><abstract>Gene transfer agents (GTAs) morphologically resemble small, double-stranded DNA (dsDNA) bacteriophages; however, their only known role is to package and transfer random pieces of the producing cell genome to recipient cells. The best understood GTA is that of Rhodobacter capsulatus, termed RcGTA. We discovered that homologues of three genes involved in natural transformation in other bacteria, comEC, comF, and comM, are essential for RcGTA-mediated gene acquisition. This paper gives genetic and biochemical evidence that RcGTA-borne DNA entry into cells requires the ComEC and ComF putative DNA transport proteins and genetic evidence that putative cytoplasmic ComM protein of unknown function is required for recipient capability. Furthermore, the master regulator of RcGTA production in <1% of a cell population, CtrA, which is also required for gene acquisition in recipient cells, is expressed in the vast majority of the population. Our results indicate that RcGTA-mediated gene transfer combines key aspects of two bacterial horizontal gene transfer mechanisms, where donor DNA is packaged in transducing phage-like particles and recipient cells take up DNA using natural transformation-related machinery. Both of these differentiated subsets of a culture population, donors and recipients, are dependent on the same response regulator, CtrA.
Horizontal gene transfer (HGT) is a major driver of bacterial evolution and adaptation to environmental stresses. Traits such as antibiotic resistance or metabolic properties can be transferred between bacteria via HGT; thus, HGT can have a tremendous effect on the fitness of a bacterial population. The three classically described HGT mechanisms are conjugation, transformation, and phage-mediated transduction. More recently, the HGT factor GTA was described, where random pieces of producing cell genome are packaged into phage-like particles that deliver DNA to recipient cells. In this report, we show that transport of DNA borne by the R. capsulatus RcGTA into recipient cells requires key genes previously thought to be specific to natural transformation pathways. These findings indicate that RcGTA combines central aspects of phage-mediated transduction and natural transformation in an efficient, regulated mode of HGT.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>26031909</pmid><doi>10.1128/JB.00332-15</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacterial proteins Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Bacteriophages - genetics Computational Biology Deoxyribonucleic acid DNA DNA, Bacterial - genetics Gene Expression Regulation, Bacterial Gene Transfer, Horizontal Genetics Gram-negative bacteria Plasmids - genetics Recombinant Proteins - genetics Rhodobacter capsulatus Rhodobacter capsulatus - genetics Spotlight |
| title | Homologues of Genetic Transformation DNA Import Genes Are Required for Rhodobacter capsulatus Gene Transfer Agent Recipient Capability Regulated by the Response Regulator CtrA |
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