Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone
The 2013–2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patien...
Gespeichert in:
| Veröffentlicht in: | Cell 2015-06, Vol.161 (7), p.1516-1526 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1526 |
|---|---|
| container_issue | 7 |
| container_start_page | 1516 |
| container_title | Cell |
| container_volume | 161 |
| creator | Park, Daniel J. Dudas, Gytis Wohl, Shirlee Goba, Augustine Whitmer, Shannon L.M. Andersen, Kristian G. Sealfon, Rachel S. Ladner, Jason T. Kugelman, Jeffrey R. Matranga, Christian B. Winnicki, Sarah M. Qu, James Gire, Stephen K. Gladden-Young, Adrianne Jalloh, Simbirie Nosamiefan, Dolo Yozwiak, Nathan L. Moses, Lina M. Jiang, Pan-Pan Lin, Aaron E. Schaffner, Stephen F. Bird, Brian Towner, Jonathan Mamoh, Mambu Gbakie, Michael Kanneh, Lansana Kargbo, David Massally, James L.B. Kamara, Fatima K. Konuwa, Edwin Sellu, Josephine Jalloh, Abdul A. Mustapha, Ibrahim Foday, Momoh Yillah, Mohamed Erickson, Bobbie R. Sealy, Tara Blau, Dianna Paddock, Christopher Brault, Aaron Amman, Brian Basile, Jane Bearden, Scott Belser, Jessica Bergeron, Eric Campbell, Shelley Chakrabarti, Ayan Dodd, Kimberly Flint, Mike Gibbons, Aridth Goodman, Christin Klena, John McMullan, Laura Morgan, Laura Russell, Brandy Salzer, Johanna Sanchez, Angela Wang, David Jungreis, Irwin Tomkins-Tinch, Christopher Kislyuk, Andrey Lin, Michael F. Chapman, Sinead MacInnis, Bronwyn Matthews, Ashley Bochicchio, James Hensley, Lisa E. Kuhn, Jens H. Nusbaum, Chad Schieffelin, John S. Birren, Bruce W. Forget, Marc Nichol, Stuart T. Palacios, Gustavo F. Ndiaye, Daouda Happi, Christian Gevao, Sahr M. Vandi, Mohamed A. Kargbo, Brima Holmes, Edward C. Bedford, Trevor Gnirke, Andreas Ströher, Ute Rambaut, Andrew Garry, Robert F. Sabeti, Pardis C. |
| description | The 2013–2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evidence for import or export of EBOV across national borders after its initial introduction. Using high-depth replicate sequencing, we observe both host-to-host transmission and recurrent emergence of intrahost genetic variants. We trace the increasing impact of purifying selection in suppressing the accumulation of nonsynonymous mutations over time. Finally, we note changes in the mucin-like domain of EBOV glycoprotein that merit further investigation. These findings clarify the movement of EBOV within the region and describe viral evolution during prolonged human-to-human transmission.
[Display omitted]
•In Sierra Leone, transmission has primarily been within-country, not between-country•Infectious doses are large enough for intrahost variants to transmit between hosts•A prolonged epidemic removes deleterious mutations from the viral population•There is preliminary evidence for human RNA editing effects on the Ebola genome
Ebola virus genomes from 232 patients sampled over 7 months in Sierra Leone were sequenced. Transmission of intrahost genetic variants suggests a sufficiently high infectious dose during transmission. The human host may have caused direct alterations to the Ebola virus genome. |
| doi_str_mv | 10.1016/j.cell.2015.06.007 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4503805</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S009286741500690X</els_id><sourcerecordid>1690215439</sourcerecordid><originalsourceid>FETCH-LOGICAL-c488t-e9b0d31433f4cb5ebf248e65cd0d16871fa022f36f8324c1a80814aa3c3c750f3</originalsourceid><addsrcrecordid>eNqFkU-LFDEQxYMo7uzqF_AgOXrYbiv_utMggiyzKox42FXwFDLp6tkMPcmYdA_stzfDrIte9BSK_N6j6j1CXjGoGbDm7bZ2OI41B6ZqaGqA9glZMOjaSrKWPyULgI5XumnlGTnPeQsAWin1nJzxBjoGolmQH8t1HC397tOc6XLve9z5OMbN_SW9TTbknc_Zx3BJbejp8hDHeSoj7efkw4be4AED_RLDdJepD_TGY0qWrjAGfEGeDXbM-PLhvSDfrpe3V5-q1dePn68-rContZ4q7NbQCyaFGKRbK1wPXGpslOuhZ41u2WCB80E0gxZcOmY1aCatFU64VsEgLsj7k-9-Xu-wdximZEezT35n072J1pu_f4K_M5t4MFKB0KCKwZsHgxR_zpgnU44-JmsDxjkbXnLjnGkh_4uypgPOlBRdQfkJdSnmnHB43IiBOdZntuaoNMf6DDSm1FdEr_-85VHyu68CvDsBWBI9lLRNdh6Dw94ndJPpo_-X_y_Zq6xO</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1690215439</pqid></control><display><type>article</type><title>Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone</title><source>MEDLINE</source><source>Cell Press Free Archives</source><source>Elsevier ScienceDirect Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Park, Daniel J. ; Dudas, Gytis ; Wohl, Shirlee ; Goba, Augustine ; Whitmer, Shannon L.M. ; Andersen, Kristian G. ; Sealfon, Rachel S. ; Ladner, Jason T. ; Kugelman, Jeffrey R. ; Matranga, Christian B. ; Winnicki, Sarah M. ; Qu, James ; Gire, Stephen K. ; Gladden-Young, Adrianne ; Jalloh, Simbirie ; Nosamiefan, Dolo ; Yozwiak, Nathan L. ; Moses, Lina M. ; Jiang, Pan-Pan ; Lin, Aaron E. ; Schaffner, Stephen F. ; Bird, Brian ; Towner, Jonathan ; Mamoh, Mambu ; Gbakie, Michael ; Kanneh, Lansana ; Kargbo, David ; Massally, James L.B. ; Kamara, Fatima K. ; Konuwa, Edwin ; Sellu, Josephine ; Jalloh, Abdul A. ; Mustapha, Ibrahim ; Foday, Momoh ; Yillah, Mohamed ; Erickson, Bobbie R. ; Sealy, Tara ; Blau, Dianna ; Paddock, Christopher ; Brault, Aaron ; Amman, Brian ; Basile, Jane ; Bearden, Scott ; Belser, Jessica ; Bergeron, Eric ; Campbell, Shelley ; Chakrabarti, Ayan ; Dodd, Kimberly ; Flint, Mike ; Gibbons, Aridth ; Goodman, Christin ; Klena, John ; McMullan, Laura ; Morgan, Laura ; Russell, Brandy ; Salzer, Johanna ; Sanchez, Angela ; Wang, David ; Jungreis, Irwin ; Tomkins-Tinch, Christopher ; Kislyuk, Andrey ; Lin, Michael F. ; Chapman, Sinead ; MacInnis, Bronwyn ; Matthews, Ashley ; Bochicchio, James ; Hensley, Lisa E. ; Kuhn, Jens H. ; Nusbaum, Chad ; Schieffelin, John S. ; Birren, Bruce W. ; Forget, Marc ; Nichol, Stuart T. ; Palacios, Gustavo F. ; Ndiaye, Daouda ; Happi, Christian ; Gevao, Sahr M. ; Vandi, Mohamed A. ; Kargbo, Brima ; Holmes, Edward C. ; Bedford, Trevor ; Gnirke, Andreas ; Ströher, Ute ; Rambaut, Andrew ; Garry, Robert F. ; Sabeti, Pardis C.</creator><creatorcontrib>Park, Daniel J. ; Dudas, Gytis ; Wohl, Shirlee ; Goba, Augustine ; Whitmer, Shannon L.M. ; Andersen, Kristian G. ; Sealfon, Rachel S. ; Ladner, Jason T. ; Kugelman, Jeffrey R. ; Matranga, Christian B. ; Winnicki, Sarah M. ; Qu, James ; Gire, Stephen K. ; Gladden-Young, Adrianne ; Jalloh, Simbirie ; Nosamiefan, Dolo ; Yozwiak, Nathan L. ; Moses, Lina M. ; Jiang, Pan-Pan ; Lin, Aaron E. ; Schaffner, Stephen F. ; Bird, Brian ; Towner, Jonathan ; Mamoh, Mambu ; Gbakie, Michael ; Kanneh, Lansana ; Kargbo, David ; Massally, James L.B. ; Kamara, Fatima K. ; Konuwa, Edwin ; Sellu, Josephine ; Jalloh, Abdul A. ; Mustapha, Ibrahim ; Foday, Momoh ; Yillah, Mohamed ; Erickson, Bobbie R. ; Sealy, Tara ; Blau, Dianna ; Paddock, Christopher ; Brault, Aaron ; Amman, Brian ; Basile, Jane ; Bearden, Scott ; Belser, Jessica ; Bergeron, Eric ; Campbell, Shelley ; Chakrabarti, Ayan ; Dodd, Kimberly ; Flint, Mike ; Gibbons, Aridth ; Goodman, Christin ; Klena, John ; McMullan, Laura ; Morgan, Laura ; Russell, Brandy ; Salzer, Johanna ; Sanchez, Angela ; Wang, David ; Jungreis, Irwin ; Tomkins-Tinch, Christopher ; Kislyuk, Andrey ; Lin, Michael F. ; Chapman, Sinead ; MacInnis, Bronwyn ; Matthews, Ashley ; Bochicchio, James ; Hensley, Lisa E. ; Kuhn, Jens H. ; Nusbaum, Chad ; Schieffelin, John S. ; Birren, Bruce W. ; Forget, Marc ; Nichol, Stuart T. ; Palacios, Gustavo F. ; Ndiaye, Daouda ; Happi, Christian ; Gevao, Sahr M. ; Vandi, Mohamed A. ; Kargbo, Brima ; Holmes, Edward C. ; Bedford, Trevor ; Gnirke, Andreas ; Ströher, Ute ; Rambaut, Andrew ; Garry, Robert F. ; Sabeti, Pardis C.</creatorcontrib><description>The 2013–2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evidence for import or export of EBOV across national borders after its initial introduction. Using high-depth replicate sequencing, we observe both host-to-host transmission and recurrent emergence of intrahost genetic variants. We trace the increasing impact of purifying selection in suppressing the accumulation of nonsynonymous mutations over time. Finally, we note changes in the mucin-like domain of EBOV glycoprotein that merit further investigation. These findings clarify the movement of EBOV within the region and describe viral evolution during prolonged human-to-human transmission.
[Display omitted]
•In Sierra Leone, transmission has primarily been within-country, not between-country•Infectious doses are large enough for intrahost variants to transmit between hosts•A prolonged epidemic removes deleterious mutations from the viral population•There is preliminary evidence for human RNA editing effects on the Ebola genome
Ebola virus genomes from 232 patients sampled over 7 months in Sierra Leone were sequenced. Transmission of intrahost genetic variants suggests a sufficiently high infectious dose during transmission. The human host may have caused direct alterations to the Ebola virus genome.</description><identifier>ISSN: 0092-8674</identifier><identifier>ISSN: 1097-4172</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/j.cell.2015.06.007</identifier><identifier>PMID: 26091036</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Biological Evolution ; Disease Outbreaks ; disease transmission ; Ebolavirus ; Ebolavirus - classification ; Ebolavirus - genetics ; Ebolavirus - isolation & purification ; evolution ; genetic variation ; genome ; Genome, Viral ; glycoproteins ; Hemorrhagic Fever, Ebola - epidemiology ; Hemorrhagic Fever, Ebola - transmission ; Hemorrhagic Fever, Ebola - virology ; Humans ; Mutation ; patients ; sequence analysis ; Sierra Leone ; Sierra Leone - epidemiology ; Specimen Handling ; viruses</subject><ispartof>Cell, 2015-06, Vol.161 (7), p.1516-1526</ispartof><rights>2015 The Authors</rights><rights>Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.</rights><rights>2015 The Authors 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-e9b0d31433f4cb5ebf248e65cd0d16871fa022f36f8324c1a80814aa3c3c750f3</citedby><cites>FETCH-LOGICAL-c488t-e9b0d31433f4cb5ebf248e65cd0d16871fa022f36f8324c1a80814aa3c3c750f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S009286741500690X$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26091036$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Daniel J.</creatorcontrib><creatorcontrib>Dudas, Gytis</creatorcontrib><creatorcontrib>Wohl, Shirlee</creatorcontrib><creatorcontrib>Goba, Augustine</creatorcontrib><creatorcontrib>Whitmer, Shannon L.M.</creatorcontrib><creatorcontrib>Andersen, Kristian G.</creatorcontrib><creatorcontrib>Sealfon, Rachel S.</creatorcontrib><creatorcontrib>Ladner, Jason T.</creatorcontrib><creatorcontrib>Kugelman, Jeffrey R.</creatorcontrib><creatorcontrib>Matranga, Christian B.</creatorcontrib><creatorcontrib>Winnicki, Sarah M.</creatorcontrib><creatorcontrib>Qu, James</creatorcontrib><creatorcontrib>Gire, Stephen K.</creatorcontrib><creatorcontrib>Gladden-Young, Adrianne</creatorcontrib><creatorcontrib>Jalloh, Simbirie</creatorcontrib><creatorcontrib>Nosamiefan, Dolo</creatorcontrib><creatorcontrib>Yozwiak, Nathan L.</creatorcontrib><creatorcontrib>Moses, Lina M.</creatorcontrib><creatorcontrib>Jiang, Pan-Pan</creatorcontrib><creatorcontrib>Lin, Aaron E.</creatorcontrib><creatorcontrib>Schaffner, Stephen F.</creatorcontrib><creatorcontrib>Bird, Brian</creatorcontrib><creatorcontrib>Towner, Jonathan</creatorcontrib><creatorcontrib>Mamoh, Mambu</creatorcontrib><creatorcontrib>Gbakie, Michael</creatorcontrib><creatorcontrib>Kanneh, Lansana</creatorcontrib><creatorcontrib>Kargbo, David</creatorcontrib><creatorcontrib>Massally, James L.B.</creatorcontrib><creatorcontrib>Kamara, Fatima K.</creatorcontrib><creatorcontrib>Konuwa, Edwin</creatorcontrib><creatorcontrib>Sellu, Josephine</creatorcontrib><creatorcontrib>Jalloh, Abdul A.</creatorcontrib><creatorcontrib>Mustapha, Ibrahim</creatorcontrib><creatorcontrib>Foday, Momoh</creatorcontrib><creatorcontrib>Yillah, Mohamed</creatorcontrib><creatorcontrib>Erickson, Bobbie R.</creatorcontrib><creatorcontrib>Sealy, Tara</creatorcontrib><creatorcontrib>Blau, Dianna</creatorcontrib><creatorcontrib>Paddock, Christopher</creatorcontrib><creatorcontrib>Brault, Aaron</creatorcontrib><creatorcontrib>Amman, Brian</creatorcontrib><creatorcontrib>Basile, Jane</creatorcontrib><creatorcontrib>Bearden, Scott</creatorcontrib><creatorcontrib>Belser, Jessica</creatorcontrib><creatorcontrib>Bergeron, Eric</creatorcontrib><creatorcontrib>Campbell, Shelley</creatorcontrib><creatorcontrib>Chakrabarti, Ayan</creatorcontrib><creatorcontrib>Dodd, Kimberly</creatorcontrib><creatorcontrib>Flint, Mike</creatorcontrib><creatorcontrib>Gibbons, Aridth</creatorcontrib><creatorcontrib>Goodman, Christin</creatorcontrib><creatorcontrib>Klena, John</creatorcontrib><creatorcontrib>McMullan, Laura</creatorcontrib><creatorcontrib>Morgan, Laura</creatorcontrib><creatorcontrib>Russell, Brandy</creatorcontrib><creatorcontrib>Salzer, Johanna</creatorcontrib><creatorcontrib>Sanchez, Angela</creatorcontrib><creatorcontrib>Wang, David</creatorcontrib><creatorcontrib>Jungreis, Irwin</creatorcontrib><creatorcontrib>Tomkins-Tinch, Christopher</creatorcontrib><creatorcontrib>Kislyuk, Andrey</creatorcontrib><creatorcontrib>Lin, Michael F.</creatorcontrib><creatorcontrib>Chapman, Sinead</creatorcontrib><creatorcontrib>MacInnis, Bronwyn</creatorcontrib><creatorcontrib>Matthews, Ashley</creatorcontrib><creatorcontrib>Bochicchio, James</creatorcontrib><creatorcontrib>Hensley, Lisa E.</creatorcontrib><creatorcontrib>Kuhn, Jens H.</creatorcontrib><creatorcontrib>Nusbaum, Chad</creatorcontrib><creatorcontrib>Schieffelin, John S.</creatorcontrib><creatorcontrib>Birren, Bruce W.</creatorcontrib><creatorcontrib>Forget, Marc</creatorcontrib><creatorcontrib>Nichol, Stuart T.</creatorcontrib><creatorcontrib>Palacios, Gustavo F.</creatorcontrib><creatorcontrib>Ndiaye, Daouda</creatorcontrib><creatorcontrib>Happi, Christian</creatorcontrib><creatorcontrib>Gevao, Sahr M.</creatorcontrib><creatorcontrib>Vandi, Mohamed A.</creatorcontrib><creatorcontrib>Kargbo, Brima</creatorcontrib><creatorcontrib>Holmes, Edward C.</creatorcontrib><creatorcontrib>Bedford, Trevor</creatorcontrib><creatorcontrib>Gnirke, Andreas</creatorcontrib><creatorcontrib>Ströher, Ute</creatorcontrib><creatorcontrib>Rambaut, Andrew</creatorcontrib><creatorcontrib>Garry, Robert F.</creatorcontrib><creatorcontrib>Sabeti, Pardis C.</creatorcontrib><title>Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone</title><title>Cell</title><addtitle>Cell</addtitle><description>The 2013–2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evidence for import or export of EBOV across national borders after its initial introduction. Using high-depth replicate sequencing, we observe both host-to-host transmission and recurrent emergence of intrahost genetic variants. We trace the increasing impact of purifying selection in suppressing the accumulation of nonsynonymous mutations over time. Finally, we note changes in the mucin-like domain of EBOV glycoprotein that merit further investigation. These findings clarify the movement of EBOV within the region and describe viral evolution during prolonged human-to-human transmission.
[Display omitted]
•In Sierra Leone, transmission has primarily been within-country, not between-country•Infectious doses are large enough for intrahost variants to transmit between hosts•A prolonged epidemic removes deleterious mutations from the viral population•There is preliminary evidence for human RNA editing effects on the Ebola genome
Ebola virus genomes from 232 patients sampled over 7 months in Sierra Leone were sequenced. Transmission of intrahost genetic variants suggests a sufficiently high infectious dose during transmission. The human host may have caused direct alterations to the Ebola virus genome.</description><subject>Biological Evolution</subject><subject>Disease Outbreaks</subject><subject>disease transmission</subject><subject>Ebolavirus</subject><subject>Ebolavirus - classification</subject><subject>Ebolavirus - genetics</subject><subject>Ebolavirus - isolation & purification</subject><subject>evolution</subject><subject>genetic variation</subject><subject>genome</subject><subject>Genome, Viral</subject><subject>glycoproteins</subject><subject>Hemorrhagic Fever, Ebola - epidemiology</subject><subject>Hemorrhagic Fever, Ebola - transmission</subject><subject>Hemorrhagic Fever, Ebola - virology</subject><subject>Humans</subject><subject>Mutation</subject><subject>patients</subject><subject>sequence analysis</subject><subject>Sierra Leone</subject><subject>Sierra Leone - epidemiology</subject><subject>Specimen Handling</subject><subject>viruses</subject><issn>0092-8674</issn><issn>1097-4172</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo7uzqF_AgOXrYbiv_utMggiyzKox42FXwFDLp6tkMPcmYdA_stzfDrIte9BSK_N6j6j1CXjGoGbDm7bZ2OI41B6ZqaGqA9glZMOjaSrKWPyULgI5XumnlGTnPeQsAWin1nJzxBjoGolmQH8t1HC397tOc6XLve9z5OMbN_SW9TTbknc_Zx3BJbejp8hDHeSoj7efkw4be4AED_RLDdJepD_TGY0qWrjAGfEGeDXbM-PLhvSDfrpe3V5-q1dePn68-rContZ4q7NbQCyaFGKRbK1wPXGpslOuhZ41u2WCB80E0gxZcOmY1aCatFU64VsEgLsj7k-9-Xu-wdximZEezT35n072J1pu_f4K_M5t4MFKB0KCKwZsHgxR_zpgnU44-JmsDxjkbXnLjnGkh_4uypgPOlBRdQfkJdSnmnHB43IiBOdZntuaoNMf6DDSm1FdEr_-85VHyu68CvDsBWBI9lLRNdh6Dw94ndJPpo_-X_y_Zq6xO</recordid><startdate>20150618</startdate><enddate>20150618</enddate><creator>Park, Daniel J.</creator><creator>Dudas, Gytis</creator><creator>Wohl, Shirlee</creator><creator>Goba, Augustine</creator><creator>Whitmer, Shannon L.M.</creator><creator>Andersen, Kristian G.</creator><creator>Sealfon, Rachel S.</creator><creator>Ladner, Jason T.</creator><creator>Kugelman, Jeffrey R.</creator><creator>Matranga, Christian B.</creator><creator>Winnicki, Sarah M.</creator><creator>Qu, James</creator><creator>Gire, Stephen K.</creator><creator>Gladden-Young, Adrianne</creator><creator>Jalloh, Simbirie</creator><creator>Nosamiefan, Dolo</creator><creator>Yozwiak, Nathan L.</creator><creator>Moses, Lina M.</creator><creator>Jiang, Pan-Pan</creator><creator>Lin, Aaron E.</creator><creator>Schaffner, Stephen F.</creator><creator>Bird, Brian</creator><creator>Towner, Jonathan</creator><creator>Mamoh, Mambu</creator><creator>Gbakie, Michael</creator><creator>Kanneh, Lansana</creator><creator>Kargbo, David</creator><creator>Massally, James L.B.</creator><creator>Kamara, Fatima K.</creator><creator>Konuwa, Edwin</creator><creator>Sellu, Josephine</creator><creator>Jalloh, Abdul A.</creator><creator>Mustapha, Ibrahim</creator><creator>Foday, Momoh</creator><creator>Yillah, Mohamed</creator><creator>Erickson, Bobbie R.</creator><creator>Sealy, Tara</creator><creator>Blau, Dianna</creator><creator>Paddock, Christopher</creator><creator>Brault, Aaron</creator><creator>Amman, Brian</creator><creator>Basile, Jane</creator><creator>Bearden, Scott</creator><creator>Belser, Jessica</creator><creator>Bergeron, Eric</creator><creator>Campbell, Shelley</creator><creator>Chakrabarti, Ayan</creator><creator>Dodd, Kimberly</creator><creator>Flint, Mike</creator><creator>Gibbons, Aridth</creator><creator>Goodman, Christin</creator><creator>Klena, John</creator><creator>McMullan, Laura</creator><creator>Morgan, Laura</creator><creator>Russell, Brandy</creator><creator>Salzer, Johanna</creator><creator>Sanchez, Angela</creator><creator>Wang, David</creator><creator>Jungreis, Irwin</creator><creator>Tomkins-Tinch, Christopher</creator><creator>Kislyuk, Andrey</creator><creator>Lin, Michael F.</creator><creator>Chapman, Sinead</creator><creator>MacInnis, Bronwyn</creator><creator>Matthews, Ashley</creator><creator>Bochicchio, James</creator><creator>Hensley, Lisa E.</creator><creator>Kuhn, Jens H.</creator><creator>Nusbaum, Chad</creator><creator>Schieffelin, John S.</creator><creator>Birren, Bruce W.</creator><creator>Forget, Marc</creator><creator>Nichol, Stuart T.</creator><creator>Palacios, Gustavo F.</creator><creator>Ndiaye, Daouda</creator><creator>Happi, Christian</creator><creator>Gevao, Sahr M.</creator><creator>Vandi, Mohamed A.</creator><creator>Kargbo, Brima</creator><creator>Holmes, Edward C.</creator><creator>Bedford, Trevor</creator><creator>Gnirke, Andreas</creator><creator>Ströher, Ute</creator><creator>Rambaut, Andrew</creator><creator>Garry, Robert F.</creator><creator>Sabeti, Pardis C.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>20150618</creationdate><title>Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone</title><author>Park, Daniel J. ; Dudas, Gytis ; Wohl, Shirlee ; Goba, Augustine ; Whitmer, Shannon L.M. ; Andersen, Kristian G. ; Sealfon, Rachel S. ; Ladner, Jason T. ; Kugelman, Jeffrey R. ; Matranga, Christian B. ; Winnicki, Sarah M. ; Qu, James ; Gire, Stephen K. ; Gladden-Young, Adrianne ; Jalloh, Simbirie ; Nosamiefan, Dolo ; Yozwiak, Nathan L. ; Moses, Lina M. ; Jiang, Pan-Pan ; Lin, Aaron E. ; Schaffner, Stephen F. ; Bird, Brian ; Towner, Jonathan ; Mamoh, Mambu ; Gbakie, Michael ; Kanneh, Lansana ; Kargbo, David ; Massally, James L.B. ; Kamara, Fatima K. ; Konuwa, Edwin ; Sellu, Josephine ; Jalloh, Abdul A. ; Mustapha, Ibrahim ; Foday, Momoh ; Yillah, Mohamed ; Erickson, Bobbie R. ; Sealy, Tara ; Blau, Dianna ; Paddock, Christopher ; Brault, Aaron ; Amman, Brian ; Basile, Jane ; Bearden, Scott ; Belser, Jessica ; Bergeron, Eric ; Campbell, Shelley ; Chakrabarti, Ayan ; Dodd, Kimberly ; Flint, Mike ; Gibbons, Aridth ; Goodman, Christin ; Klena, John ; McMullan, Laura ; Morgan, Laura ; Russell, Brandy ; Salzer, Johanna ; Sanchez, Angela ; Wang, David ; Jungreis, Irwin ; Tomkins-Tinch, Christopher ; Kislyuk, Andrey ; Lin, Michael F. ; Chapman, Sinead ; MacInnis, Bronwyn ; Matthews, Ashley ; Bochicchio, James ; Hensley, Lisa E. ; Kuhn, Jens H. ; Nusbaum, Chad ; Schieffelin, John S. ; Birren, Bruce W. ; Forget, Marc ; Nichol, Stuart T. ; Palacios, Gustavo F. ; Ndiaye, Daouda ; Happi, Christian ; Gevao, Sahr M. ; Vandi, Mohamed A. ; Kargbo, Brima ; Holmes, Edward C. ; Bedford, Trevor ; Gnirke, Andreas ; Ströher, Ute ; Rambaut, Andrew ; Garry, Robert F. ; Sabeti, Pardis C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-e9b0d31433f4cb5ebf248e65cd0d16871fa022f36f8324c1a80814aa3c3c750f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biological Evolution</topic><topic>Disease Outbreaks</topic><topic>disease transmission</topic><topic>Ebolavirus</topic><topic>Ebolavirus - classification</topic><topic>Ebolavirus - genetics</topic><topic>Ebolavirus - isolation & purification</topic><topic>evolution</topic><topic>genetic variation</topic><topic>genome</topic><topic>Genome, Viral</topic><topic>glycoproteins</topic><topic>Hemorrhagic Fever, Ebola - epidemiology</topic><topic>Hemorrhagic Fever, Ebola - transmission</topic><topic>Hemorrhagic Fever, Ebola - virology</topic><topic>Humans</topic><topic>Mutation</topic><topic>patients</topic><topic>sequence analysis</topic><topic>Sierra Leone</topic><topic>Sierra Leone - epidemiology</topic><topic>Specimen Handling</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Daniel J.</creatorcontrib><creatorcontrib>Dudas, Gytis</creatorcontrib><creatorcontrib>Wohl, Shirlee</creatorcontrib><creatorcontrib>Goba, Augustine</creatorcontrib><creatorcontrib>Whitmer, Shannon L.M.</creatorcontrib><creatorcontrib>Andersen, Kristian G.</creatorcontrib><creatorcontrib>Sealfon, Rachel S.</creatorcontrib><creatorcontrib>Ladner, Jason T.</creatorcontrib><creatorcontrib>Kugelman, Jeffrey R.</creatorcontrib><creatorcontrib>Matranga, Christian B.</creatorcontrib><creatorcontrib>Winnicki, Sarah M.</creatorcontrib><creatorcontrib>Qu, James</creatorcontrib><creatorcontrib>Gire, Stephen K.</creatorcontrib><creatorcontrib>Gladden-Young, Adrianne</creatorcontrib><creatorcontrib>Jalloh, Simbirie</creatorcontrib><creatorcontrib>Nosamiefan, Dolo</creatorcontrib><creatorcontrib>Yozwiak, Nathan L.</creatorcontrib><creatorcontrib>Moses, Lina M.</creatorcontrib><creatorcontrib>Jiang, Pan-Pan</creatorcontrib><creatorcontrib>Lin, Aaron E.</creatorcontrib><creatorcontrib>Schaffner, Stephen F.</creatorcontrib><creatorcontrib>Bird, Brian</creatorcontrib><creatorcontrib>Towner, Jonathan</creatorcontrib><creatorcontrib>Mamoh, Mambu</creatorcontrib><creatorcontrib>Gbakie, Michael</creatorcontrib><creatorcontrib>Kanneh, Lansana</creatorcontrib><creatorcontrib>Kargbo, David</creatorcontrib><creatorcontrib>Massally, James L.B.</creatorcontrib><creatorcontrib>Kamara, Fatima K.</creatorcontrib><creatorcontrib>Konuwa, Edwin</creatorcontrib><creatorcontrib>Sellu, Josephine</creatorcontrib><creatorcontrib>Jalloh, Abdul A.</creatorcontrib><creatorcontrib>Mustapha, Ibrahim</creatorcontrib><creatorcontrib>Foday, Momoh</creatorcontrib><creatorcontrib>Yillah, Mohamed</creatorcontrib><creatorcontrib>Erickson, Bobbie R.</creatorcontrib><creatorcontrib>Sealy, Tara</creatorcontrib><creatorcontrib>Blau, Dianna</creatorcontrib><creatorcontrib>Paddock, Christopher</creatorcontrib><creatorcontrib>Brault, Aaron</creatorcontrib><creatorcontrib>Amman, Brian</creatorcontrib><creatorcontrib>Basile, Jane</creatorcontrib><creatorcontrib>Bearden, Scott</creatorcontrib><creatorcontrib>Belser, Jessica</creatorcontrib><creatorcontrib>Bergeron, Eric</creatorcontrib><creatorcontrib>Campbell, Shelley</creatorcontrib><creatorcontrib>Chakrabarti, Ayan</creatorcontrib><creatorcontrib>Dodd, Kimberly</creatorcontrib><creatorcontrib>Flint, Mike</creatorcontrib><creatorcontrib>Gibbons, Aridth</creatorcontrib><creatorcontrib>Goodman, Christin</creatorcontrib><creatorcontrib>Klena, John</creatorcontrib><creatorcontrib>McMullan, Laura</creatorcontrib><creatorcontrib>Morgan, Laura</creatorcontrib><creatorcontrib>Russell, Brandy</creatorcontrib><creatorcontrib>Salzer, Johanna</creatorcontrib><creatorcontrib>Sanchez, Angela</creatorcontrib><creatorcontrib>Wang, David</creatorcontrib><creatorcontrib>Jungreis, Irwin</creatorcontrib><creatorcontrib>Tomkins-Tinch, Christopher</creatorcontrib><creatorcontrib>Kislyuk, Andrey</creatorcontrib><creatorcontrib>Lin, Michael F.</creatorcontrib><creatorcontrib>Chapman, Sinead</creatorcontrib><creatorcontrib>MacInnis, Bronwyn</creatorcontrib><creatorcontrib>Matthews, Ashley</creatorcontrib><creatorcontrib>Bochicchio, James</creatorcontrib><creatorcontrib>Hensley, Lisa E.</creatorcontrib><creatorcontrib>Kuhn, Jens H.</creatorcontrib><creatorcontrib>Nusbaum, Chad</creatorcontrib><creatorcontrib>Schieffelin, John S.</creatorcontrib><creatorcontrib>Birren, Bruce W.</creatorcontrib><creatorcontrib>Forget, Marc</creatorcontrib><creatorcontrib>Nichol, Stuart T.</creatorcontrib><creatorcontrib>Palacios, Gustavo F.</creatorcontrib><creatorcontrib>Ndiaye, Daouda</creatorcontrib><creatorcontrib>Happi, Christian</creatorcontrib><creatorcontrib>Gevao, Sahr M.</creatorcontrib><creatorcontrib>Vandi, Mohamed A.</creatorcontrib><creatorcontrib>Kargbo, Brima</creatorcontrib><creatorcontrib>Holmes, Edward C.</creatorcontrib><creatorcontrib>Bedford, Trevor</creatorcontrib><creatorcontrib>Gnirke, Andreas</creatorcontrib><creatorcontrib>Ströher, Ute</creatorcontrib><creatorcontrib>Rambaut, Andrew</creatorcontrib><creatorcontrib>Garry, Robert F.</creatorcontrib><creatorcontrib>Sabeti, Pardis C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Daniel J.</au><au>Dudas, Gytis</au><au>Wohl, Shirlee</au><au>Goba, Augustine</au><au>Whitmer, Shannon L.M.</au><au>Andersen, Kristian G.</au><au>Sealfon, Rachel S.</au><au>Ladner, Jason T.</au><au>Kugelman, Jeffrey R.</au><au>Matranga, Christian B.</au><au>Winnicki, Sarah M.</au><au>Qu, James</au><au>Gire, Stephen K.</au><au>Gladden-Young, Adrianne</au><au>Jalloh, Simbirie</au><au>Nosamiefan, Dolo</au><au>Yozwiak, Nathan L.</au><au>Moses, Lina M.</au><au>Jiang, Pan-Pan</au><au>Lin, Aaron E.</au><au>Schaffner, Stephen F.</au><au>Bird, Brian</au><au>Towner, Jonathan</au><au>Mamoh, Mambu</au><au>Gbakie, Michael</au><au>Kanneh, Lansana</au><au>Kargbo, David</au><au>Massally, James L.B.</au><au>Kamara, Fatima K.</au><au>Konuwa, Edwin</au><au>Sellu, Josephine</au><au>Jalloh, Abdul A.</au><au>Mustapha, Ibrahim</au><au>Foday, Momoh</au><au>Yillah, Mohamed</au><au>Erickson, Bobbie R.</au><au>Sealy, Tara</au><au>Blau, Dianna</au><au>Paddock, Christopher</au><au>Brault, Aaron</au><au>Amman, Brian</au><au>Basile, Jane</au><au>Bearden, Scott</au><au>Belser, Jessica</au><au>Bergeron, Eric</au><au>Campbell, Shelley</au><au>Chakrabarti, Ayan</au><au>Dodd, Kimberly</au><au>Flint, Mike</au><au>Gibbons, Aridth</au><au>Goodman, Christin</au><au>Klena, John</au><au>McMullan, Laura</au><au>Morgan, Laura</au><au>Russell, Brandy</au><au>Salzer, Johanna</au><au>Sanchez, Angela</au><au>Wang, David</au><au>Jungreis, Irwin</au><au>Tomkins-Tinch, Christopher</au><au>Kislyuk, Andrey</au><au>Lin, Michael F.</au><au>Chapman, Sinead</au><au>MacInnis, Bronwyn</au><au>Matthews, Ashley</au><au>Bochicchio, James</au><au>Hensley, Lisa E.</au><au>Kuhn, Jens H.</au><au>Nusbaum, Chad</au><au>Schieffelin, John S.</au><au>Birren, Bruce W.</au><au>Forget, Marc</au><au>Nichol, Stuart T.</au><au>Palacios, Gustavo F.</au><au>Ndiaye, Daouda</au><au>Happi, Christian</au><au>Gevao, Sahr M.</au><au>Vandi, Mohamed A.</au><au>Kargbo, Brima</au><au>Holmes, Edward C.</au><au>Bedford, Trevor</au><au>Gnirke, Andreas</au><au>Ströher, Ute</au><au>Rambaut, Andrew</au><au>Garry, Robert F.</au><au>Sabeti, Pardis C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>2015-06-18</date><risdate>2015</risdate><volume>161</volume><issue>7</issue><spage>1516</spage><epage>1526</epage><pages>1516-1526</pages><issn>0092-8674</issn><issn>1097-4172</issn><eissn>1097-4172</eissn><abstract>The 2013–2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evidence for import or export of EBOV across national borders after its initial introduction. Using high-depth replicate sequencing, we observe both host-to-host transmission and recurrent emergence of intrahost genetic variants. We trace the increasing impact of purifying selection in suppressing the accumulation of nonsynonymous mutations over time. Finally, we note changes in the mucin-like domain of EBOV glycoprotein that merit further investigation. These findings clarify the movement of EBOV within the region and describe viral evolution during prolonged human-to-human transmission.
[Display omitted]
•In Sierra Leone, transmission has primarily been within-country, not between-country•Infectious doses are large enough for intrahost variants to transmit between hosts•A prolonged epidemic removes deleterious mutations from the viral population•There is preliminary evidence for human RNA editing effects on the Ebola genome
Ebola virus genomes from 232 patients sampled over 7 months in Sierra Leone were sequenced. Transmission of intrahost genetic variants suggests a sufficiently high infectious dose during transmission. The human host may have caused direct alterations to the Ebola virus genome.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>26091036</pmid><doi>10.1016/j.cell.2015.06.007</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0092-8674 |
| ispartof | Cell, 2015-06, Vol.161 (7), p.1516-1526 |
| issn | 0092-8674 1097-4172 1097-4172 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4503805 |
| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Biological Evolution Disease Outbreaks disease transmission Ebolavirus Ebolavirus - classification Ebolavirus - genetics Ebolavirus - isolation & purification evolution genetic variation genome Genome, Viral glycoproteins Hemorrhagic Fever, Ebola - epidemiology Hemorrhagic Fever, Ebola - transmission Hemorrhagic Fever, Ebola - virology Humans Mutation patients sequence analysis Sierra Leone Sierra Leone - epidemiology Specimen Handling viruses |
| title | Ebola Virus Epidemiology, Transmission, and Evolution during Seven Months in Sierra Leone |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T14%3A53%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ebola%20Virus%20Epidemiology,%20Transmission,%20and%20Evolution%20during%20Seven%20Months%20in%20Sierra%20Leone&rft.jtitle=Cell&rft.au=Park,%20Daniel%C2%A0J.&rft.date=2015-06-18&rft.volume=161&rft.issue=7&rft.spage=1516&rft.epage=1526&rft.pages=1516-1526&rft.issn=0092-8674&rft.eissn=1097-4172&rft_id=info:doi/10.1016/j.cell.2015.06.007&rft_dat=%3Cproquest_pubme%3E1690215439%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1690215439&rft_id=info:pmid/26091036&rft_els_id=S009286741500690X&rfr_iscdi=true |