Enzymatically degradable poly(ethylene glycol) hydrogels for the 3D culture and release of human embryonic stem cell derived pancreatic precursor cell aggregates

[Display omitted] This study aimed to develop a three dimensional culture platform for aggregates of human embryonic stem cell (hESC)-derived pancreatic progenitors that enables long-term culture, maintains aggregate size and morphology, does not adversely affect differentiation and provides a means...

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Veröffentlicht in:	Acta biomaterialia 2015-08, Vol.22, p.103-110
Hauptverfasser:	Amer, Luke D., Holtzinger, Audrey, Keller, Gordon, Mahoney, Melissa J., Bryant, Stephanie J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aggregates Amino Acid Sequence Animals Cell Aggregation Cell Culture Techniques - methods Cell Line, Tumor Cell Size Cell Survival Controlled release Culture Encapsulation Enzymes - metabolism Flow Cytometry Human embryonic stem cells Human Embryonic Stem Cells - cytology Humans Hydrogels Hydrogels - chemistry Long-term culture platforms Mice Microscopy, Confocal Molecular Sequence Data Pancreas - cytology Pancreatic precursor cells Peptides - chemistry Platforms Poly(ethylene glycol) hydrogels Polyethylene Glycols - chemistry Polymerization Rats Recovery Three dimensional Transcription Factors - metabolism Viability
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