Activated platelet–T-cell conjugates in peripheral blood of patients with HIV infection: coupling coagulation/inflammation and T cells

BACKGROUND:Despite successfully suppressed viremia by treatment, patients with high levels of biomarkers of coagulation/inflammation are at an increased risk of developing non-AIDS defining serious illnesses such as cardiovascular diseases. Thus, there is a relationship between persistent immune act...

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Veröffentlicht in:AIDS (London) 2015-07, Vol.29 (11), p.1297-1308
Hauptverfasser: Green, Samantha A, Smith, Mindy, Hasley, Rebecca B, Stephany, David, Harned, Adam, Nagashima, Kunio, Abdullah, Shahed, Pittaluga, Stefania, Imamichi, Tomozumi, Qin, Jing, Rupert, Adam, Ober, Alex, Lane, H Clifford, Catalfamo, Marta
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container_end_page 1308
container_issue 11
container_start_page 1297
container_title AIDS (London)
container_volume 29
creator Green, Samantha A
Smith, Mindy
Hasley, Rebecca B
Stephany, David
Harned, Adam
Nagashima, Kunio
Abdullah, Shahed
Pittaluga, Stefania
Imamichi, Tomozumi
Qin, Jing
Rupert, Adam
Ober, Alex
Lane, H Clifford
Catalfamo, Marta
description BACKGROUND:Despite successfully suppressed viremia by treatment, patients with high levels of biomarkers of coagulation/inflammation are at an increased risk of developing non-AIDS defining serious illnesses such as cardiovascular diseases. Thus, there is a relationship between persistent immune activation and coagulation/inflammation, although the mechanisms are poorly understood. Platelets play an important role in this process. Although interactions between platelets and elements of the innate immune system, such as monocytes, are well described, little is known about the interaction between platelets and the adaptive immune system. DESIGN:We investigated the interaction of a component of the coagulation system, platelets, and the adaptive immune system T cells. METHODS:Healthy controls and combination antiretroviral therapy (cART)-treated HIV-infected patients with viral loads of less than 40 copies/ml for more than 15 months were analysed for platelet–T-cell conjugate formation. RESULTS:Platelets can form conjugates with T cells and were preferentially seen in CD4 and CD8 T-cell subsets with more differentiated phenotypes [memory, memory/effector and terminal effector memory (TEM)]. Compared with healthy controls, these conjugates in patients with HIV infection were more frequent, more often composed of activated platelets (CD42bCD62P), and were significantly associated with the D-dimer serum levels. CONCLUSION:These data support a model in which platelet–T-cell conjugates may play a critical role in the fast recruitment of antigen-experienced T cells to the place of injury. This mechanism can contribute in maintaining a state of coagulation/inflammation observed in these patients contributing to the pathology of the disease.
doi_str_mv 10.1097/QAD.0000000000000701
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Thus, there is a relationship between persistent immune activation and coagulation/inflammation, although the mechanisms are poorly understood. Platelets play an important role in this process. Although interactions between platelets and elements of the innate immune system, such as monocytes, are well described, little is known about the interaction between platelets and the adaptive immune system. DESIGN:We investigated the interaction of a component of the coagulation system, platelets, and the adaptive immune system T cells. METHODS:Healthy controls and combination antiretroviral therapy (cART)-treated HIV-infected patients with viral loads of less than 40 copies/ml for more than 15 months were analysed for platelet–T-cell conjugate formation. RESULTS:Platelets can form conjugates with T cells and were preferentially seen in CD4 and CD8 T-cell subsets with more differentiated phenotypes [memory, memory/effector and terminal effector memory (TEM)]. Compared with healthy controls, these conjugates in patients with HIV infection were more frequent, more often composed of activated platelets (CD42bCD62P), and were significantly associated with the D-dimer serum levels. CONCLUSION:These data support a model in which platelet–T-cell conjugates may play a critical role in the fast recruitment of antigen-experienced T cells to the place of injury. This mechanism can contribute in maintaining a state of coagulation/inflammation observed in these patients contributing to the pathology of the disease.</description><identifier>ISSN: 0269-9370</identifier><identifier>EISSN: 1473-5571</identifier><identifier>DOI: 10.1097/QAD.0000000000000701</identifier><identifier>PMID: 26002800</identifier><language>eng</language><publisher>England: Copyright Wolters Kluwer Health, Inc</publisher><subject>AIDS/HIV ; Basic Science ; Biomarkers - blood ; Blood Coagulation ; Blood Platelets - immunology ; Case-Control Studies ; CD4 Lymphocyte Count ; CD8-Positive T-Lymphocytes - immunology ; Fibrin Fibrinogen Degradation Products - analysis ; HIV Infections - blood ; Humans ; Inflammation - immunology ; Lentivirus ; Lymphocyte Activation ; Retroviridae ; T-Lymphocyte Subsets - immunology ; Viral Load ; Viremia - drug therapy</subject><ispartof>AIDS (London), 2015-07, Vol.29 (11), p.1297-1308</ispartof><rights>Copyright © 2015 Wolters Kluwer Health, Inc.</rights><rights>Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3931-92ceb69ea9aca363a0ca62c30dfaf34a2a1698fc2ed1303e65edfba3185612973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26002800$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Green, Samantha A</creatorcontrib><creatorcontrib>Smith, Mindy</creatorcontrib><creatorcontrib>Hasley, Rebecca B</creatorcontrib><creatorcontrib>Stephany, David</creatorcontrib><creatorcontrib>Harned, Adam</creatorcontrib><creatorcontrib>Nagashima, Kunio</creatorcontrib><creatorcontrib>Abdullah, Shahed</creatorcontrib><creatorcontrib>Pittaluga, Stefania</creatorcontrib><creatorcontrib>Imamichi, Tomozumi</creatorcontrib><creatorcontrib>Qin, Jing</creatorcontrib><creatorcontrib>Rupert, Adam</creatorcontrib><creatorcontrib>Ober, Alex</creatorcontrib><creatorcontrib>Lane, H Clifford</creatorcontrib><creatorcontrib>Catalfamo, Marta</creatorcontrib><title>Activated platelet–T-cell conjugates in peripheral blood of patients with HIV infection: coupling coagulation/inflammation and T cells</title><title>AIDS (London)</title><addtitle>AIDS</addtitle><description>BACKGROUND:Despite successfully suppressed viremia by treatment, patients with high levels of biomarkers of coagulation/inflammation are at an increased risk of developing non-AIDS defining serious illnesses such as cardiovascular diseases. 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Compared with healthy controls, these conjugates in patients with HIV infection were more frequent, more often composed of activated platelets (CD42bCD62P), and were significantly associated with the D-dimer serum levels. CONCLUSION:These data support a model in which platelet–T-cell conjugates may play a critical role in the fast recruitment of antigen-experienced T cells to the place of injury. 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Thus, there is a relationship between persistent immune activation and coagulation/inflammation, although the mechanisms are poorly understood. Platelets play an important role in this process. Although interactions between platelets and elements of the innate immune system, such as monocytes, are well described, little is known about the interaction between platelets and the adaptive immune system. DESIGN:We investigated the interaction of a component of the coagulation system, platelets, and the adaptive immune system T cells. METHODS:Healthy controls and combination antiretroviral therapy (cART)-treated HIV-infected patients with viral loads of less than 40 copies/ml for more than 15 months were analysed for platelet–T-cell conjugate formation. RESULTS:Platelets can form conjugates with T cells and were preferentially seen in CD4 and CD8 T-cell subsets with more differentiated phenotypes [memory, memory/effector and terminal effector memory (TEM)]. Compared with healthy controls, these conjugates in patients with HIV infection were more frequent, more often composed of activated platelets (CD42bCD62P), and were significantly associated with the D-dimer serum levels. CONCLUSION:These data support a model in which platelet–T-cell conjugates may play a critical role in the fast recruitment of antigen-experienced T cells to the place of injury. This mechanism can contribute in maintaining a state of coagulation/inflammation observed in these patients contributing to the pathology of the disease.</abstract><cop>England</cop><pub>Copyright Wolters Kluwer Health, Inc</pub><pmid>26002800</pmid><doi>10.1097/QAD.0000000000000701</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record>
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source MEDLINE; EZB-FREE-00999 freely available EZB journals; Journals@Ovid Complete
subjects AIDS/HIV
Basic Science
Biomarkers - blood
Blood Coagulation
Blood Platelets - immunology
Case-Control Studies
CD4 Lymphocyte Count
CD8-Positive T-Lymphocytes - immunology
Fibrin Fibrinogen Degradation Products - analysis
HIV Infections - blood
Humans
Inflammation - immunology
Lentivirus
Lymphocyte Activation
Retroviridae
T-Lymphocyte Subsets - immunology
Viral Load
Viremia - drug therapy
title Activated platelet–T-cell conjugates in peripheral blood of patients with HIV infection: coupling coagulation/inflammation and T cells
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