Characterization of a redox active cross-linked complex between cyanobacterial photosystem I and soluble ferredoxin

A covalent stoichiometric complex between photosystem I (PSI) and ferredoxin from the cyanobacterium Synechocystis sp. PCC 6803 was generated by chemical cross-linking. The photoreduction of ferredoxin, studied by laser flash absorption spectroscopy between 460 and 600 nm, is a fast process in 60% o...

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Veröffentlicht in:	The EMBO journal 1996-05, Vol.15 (9), p.2160-2168
Hauptverfasser:	Lelong, C, Boekema, E.J, Kruip, J, Bottin, H, Rogner, M, Setif, P
Format:	Artikel
Sprache:	eng
Schlagworte:	binding sites chemical reactions Cyanobacteria Cyanobacteria - metabolism Cyanophyta electron paramagnetic resonance spectroscopy electron transfer Electron Transport ferredoxin Ferredoxins - metabolism flash absorption spectroscopy Microscopy, Electron Oxidation-Reduction photoreduction Photosynthetic Reaction Center Complex Proteins - metabolism Photosynthetic Reaction Center Complex Proteins - ultrastructure photosystem I reduction spectral analysis spectroscopy Spectrum Analysis Synechocystis ultrastructure
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Beschreibung
Zusammenfassung:	A covalent stoichiometric complex between photosystem I (PSI) and ferredoxin from the cyanobacterium Synechocystis sp. PCC 6803 was generated by chemical cross-linking. The photoreduction of ferredoxin, studied by laser flash absorption spectroscopy between 460 and 600 nm, is a fast process in 60% of the covalent complexes, which exhibit spectral and kinetic properties very similar to those observed with the free partners. Two major phases with t1/2
ISSN:	0261-4189 1460-2075
DOI:	10.1002/j.1460-2075.1996.tb00569.x