Identification of dfrA14 in two distinct plasmids conferring trimethoprim resistance in Actinobacillus pleuropneumoniae
The objective of this study was to determine the distribution and genetic basis of trimethoprim resistance in Actinobacillus pleuropneumoniae isolates from pigs in England. Clinical isolates collected between 1998 and 2011 were tested for resistance to trimethoprim and sulphonamide. The genetic basi...
Gespeichert in:
| Veröffentlicht in: | Journal of antimicrobial chemotherapy 2015-08, Vol.70 (8), p.2217-2222 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2222 |
|---|---|
| container_issue | 8 |
| container_start_page | 2217 |
| container_title | Journal of antimicrobial chemotherapy |
| container_volume | 70 |
| creator | Bossé, Janine T Li, Yanwen Walker, Stephanie Atherton, Tom Fernandez Crespo, Roberto Williamson, Susanna M Rogers, Jon Chaudhuri, Roy R Weinert, Lucy A Oshota, Olusegun Holden, Matt T G Maskell, Duncan J Tucker, Alexander W Wren, Brendan W Rycroft, Andrew N Langford, Paul R |
| description | The objective of this study was to determine the distribution and genetic basis of trimethoprim resistance in Actinobacillus pleuropneumoniae isolates from pigs in England.
Clinical isolates collected between 1998 and 2011 were tested for resistance to trimethoprim and sulphonamide. The genetic basis of trimethoprim resistance was determined by shotgun WGS analysis and the subsequent isolation and sequencing of plasmids.
A total of 16 (out of 106) A. pleuropneumoniae isolates were resistant to both trimethoprim (MIC >32 mg/L) and sulfisoxazole (MIC ≥256 mg/L), and a further 32 were resistant only to sulfisoxazole (MIC ≥256 mg/L). Genome sequence data for the trimethoprim-resistant isolates revealed the presence of the dfrA14 dihydrofolate reductase gene. The distribution of plasmid sequences in multiple contigs suggested the presence of two distinct dfrA14-containing plasmids in different isolates, which was confirmed by plasmid isolation and sequencing. Both plasmids encoded mobilization genes, the sulphonamide resistance gene sul2, as well as dfrA14 inserted into strA, a streptomycin-resistance-associated gene, although the gene order differed between the two plasmids. One of the plasmids further encoded the strB streptomycin-resistance-associated gene.
This is the first description of mobilizable plasmids conferring trimethoprim resistance in A. pleuropneumoniae and, to our knowledge, the first report of dfrA14 in any member of the Pasteurellaceae. The identification of dfrA14 conferring trimethoprim resistance in A. pleuropneumoniae isolates will facilitate PCR screens for resistance to this important antimicrobial. |
| doi_str_mv | 10.1093/jac/dkv121 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4500777</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1696679673</sourcerecordid><originalsourceid>FETCH-LOGICAL-c439t-68f51ab040b2ddf05b922cb4ff63467098d5fbfde261321a016e395438c006c53</originalsourceid><addsrcrecordid>eNqNkUtrFEEUhQtRzCS68QdIgxsJtLn17toIQ_ARCLjRdVFdj6TG7qqxqjvBf28NE4O6cnUX9zuHe-5B6BWGdxgUvdgZe-G-32GCn6ANZgJ6Ago_RRugwHvJOD1Bp7XuAEBwMTxHJ4QrLulANuj-yvm0xBCtWWJOXQ6dC2WLWRdTt9znzsW6xGSXbj-ZOkdXO5tT8KXEdNMtJc5-uc37Nrvia2NNsv6g3domy6OxcZrW2tR-LXmf_DrnFI1_gZ4FM1X_8mGeoW8fP3y9_Nxff_l0dbm97i2jaunFEDg2IzAYiXMB-KgIsSMLQVAmJKjB8TAG54nAlGADWHiqOKODbWEtp2fo_dF3v46zd7aFLWbSh4NN-amzifrvTYq3-ibfacYBpJTN4O2DQck_Vl8XPcdq_TSZ5PNaNZaYcKEI-w9UKCGkEpI29M0_6C6vJbVPHCgJhBGARp0fKVtyrcWHx7sx6EP1ulWvj9U3-PWfSR_R313TXy27rTM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1697024200</pqid></control><display><type>article</type><title>Identification of dfrA14 in two distinct plasmids conferring trimethoprim resistance in Actinobacillus pleuropneumoniae</title><source>MEDLINE</source><source>Oxford University Press Journals Current</source><source>Full-Text Journals in Chemistry (Open access)</source><source>EZB Free E-Journals</source><source>Alma/SFX Local Collection</source><creator>Bossé, Janine T ; Li, Yanwen ; Walker, Stephanie ; Atherton, Tom ; Fernandez Crespo, Roberto ; Williamson, Susanna M ; Rogers, Jon ; Chaudhuri, Roy R ; Weinert, Lucy A ; Oshota, Olusegun ; Holden, Matt T G ; Maskell, Duncan J ; Tucker, Alexander W ; Wren, Brendan W ; Rycroft, Andrew N ; Langford, Paul R</creator><creatorcontrib>Bossé, Janine T ; Li, Yanwen ; Walker, Stephanie ; Atherton, Tom ; Fernandez Crespo, Roberto ; Williamson, Susanna M ; Rogers, Jon ; Chaudhuri, Roy R ; Weinert, Lucy A ; Oshota, Olusegun ; Holden, Matt T G ; Maskell, Duncan J ; Tucker, Alexander W ; Wren, Brendan W ; Rycroft, Andrew N ; Langford, Paul R ; BRaDP1T Consortium ; on behalf of the BRaDP1T Consortium</creatorcontrib><description>The objective of this study was to determine the distribution and genetic basis of trimethoprim resistance in Actinobacillus pleuropneumoniae isolates from pigs in England.
Clinical isolates collected between 1998 and 2011 were tested for resistance to trimethoprim and sulphonamide. The genetic basis of trimethoprim resistance was determined by shotgun WGS analysis and the subsequent isolation and sequencing of plasmids.
A total of 16 (out of 106) A. pleuropneumoniae isolates were resistant to both trimethoprim (MIC >32 mg/L) and sulfisoxazole (MIC ≥256 mg/L), and a further 32 were resistant only to sulfisoxazole (MIC ≥256 mg/L). Genome sequence data for the trimethoprim-resistant isolates revealed the presence of the dfrA14 dihydrofolate reductase gene. The distribution of plasmid sequences in multiple contigs suggested the presence of two distinct dfrA14-containing plasmids in different isolates, which was confirmed by plasmid isolation and sequencing. Both plasmids encoded mobilization genes, the sulphonamide resistance gene sul2, as well as dfrA14 inserted into strA, a streptomycin-resistance-associated gene, although the gene order differed between the two plasmids. One of the plasmids further encoded the strB streptomycin-resistance-associated gene.
This is the first description of mobilizable plasmids conferring trimethoprim resistance in A. pleuropneumoniae and, to our knowledge, the first report of dfrA14 in any member of the Pasteurellaceae. The identification of dfrA14 conferring trimethoprim resistance in A. pleuropneumoniae isolates will facilitate PCR screens for resistance to this important antimicrobial.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dkv121</identifier><identifier>PMID: 25957382</identifier><language>eng</language><publisher>England: Oxford Publishing Limited (England)</publisher><subject>Actinobacillus Infections - microbiology ; Actinobacillus Infections - veterinary ; Actinobacillus pleuropneumoniae ; Actinobacillus pleuropneumoniae - drug effects ; Actinobacillus pleuropneumoniae - enzymology ; Actinobacillus pleuropneumoniae - genetics ; Actinobacillus pleuropneumoniae - isolation & purification ; Animals ; Anti-Infective Agents - pharmacology ; Antibiotics ; Antimicrobial agents ; Drug resistance ; England ; Genome, Bacterial ; Hogs ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Original Research ; Pasteurellaceae ; Pathogens ; Plasmids ; Sequence Analysis, DNA ; Sulfisoxazole - pharmacology ; Swine ; Swine Diseases - microbiology ; Tetrahydrofolate Dehydrogenase - genetics ; Trimethoprim - pharmacology ; Trimethoprim Resistance</subject><ispartof>Journal of antimicrobial chemotherapy, 2015-08, Vol.70 (8), p.2217-2222</ispartof><rights>The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.</rights><rights>Copyright Oxford Publishing Limited(England) Aug 2015</rights><rights>The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-68f51ab040b2ddf05b922cb4ff63467098d5fbfde261321a016e395438c006c53</citedby><cites>FETCH-LOGICAL-c439t-68f51ab040b2ddf05b922cb4ff63467098d5fbfde261321a016e395438c006c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25957382$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bossé, Janine T</creatorcontrib><creatorcontrib>Li, Yanwen</creatorcontrib><creatorcontrib>Walker, Stephanie</creatorcontrib><creatorcontrib>Atherton, Tom</creatorcontrib><creatorcontrib>Fernandez Crespo, Roberto</creatorcontrib><creatorcontrib>Williamson, Susanna M</creatorcontrib><creatorcontrib>Rogers, Jon</creatorcontrib><creatorcontrib>Chaudhuri, Roy R</creatorcontrib><creatorcontrib>Weinert, Lucy A</creatorcontrib><creatorcontrib>Oshota, Olusegun</creatorcontrib><creatorcontrib>Holden, Matt T G</creatorcontrib><creatorcontrib>Maskell, Duncan J</creatorcontrib><creatorcontrib>Tucker, Alexander W</creatorcontrib><creatorcontrib>Wren, Brendan W</creatorcontrib><creatorcontrib>Rycroft, Andrew N</creatorcontrib><creatorcontrib>Langford, Paul R</creatorcontrib><creatorcontrib>BRaDP1T Consortium</creatorcontrib><creatorcontrib>on behalf of the BRaDP1T Consortium</creatorcontrib><title>Identification of dfrA14 in two distinct plasmids conferring trimethoprim resistance in Actinobacillus pleuropneumoniae</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>The objective of this study was to determine the distribution and genetic basis of trimethoprim resistance in Actinobacillus pleuropneumoniae isolates from pigs in England.
Clinical isolates collected between 1998 and 2011 were tested for resistance to trimethoprim and sulphonamide. The genetic basis of trimethoprim resistance was determined by shotgun WGS analysis and the subsequent isolation and sequencing of plasmids.
A total of 16 (out of 106) A. pleuropneumoniae isolates were resistant to both trimethoprim (MIC >32 mg/L) and sulfisoxazole (MIC ≥256 mg/L), and a further 32 were resistant only to sulfisoxazole (MIC ≥256 mg/L). Genome sequence data for the trimethoprim-resistant isolates revealed the presence of the dfrA14 dihydrofolate reductase gene. The distribution of plasmid sequences in multiple contigs suggested the presence of two distinct dfrA14-containing plasmids in different isolates, which was confirmed by plasmid isolation and sequencing. Both plasmids encoded mobilization genes, the sulphonamide resistance gene sul2, as well as dfrA14 inserted into strA, a streptomycin-resistance-associated gene, although the gene order differed between the two plasmids. One of the plasmids further encoded the strB streptomycin-resistance-associated gene.
This is the first description of mobilizable plasmids conferring trimethoprim resistance in A. pleuropneumoniae and, to our knowledge, the first report of dfrA14 in any member of the Pasteurellaceae. The identification of dfrA14 conferring trimethoprim resistance in A. pleuropneumoniae isolates will facilitate PCR screens for resistance to this important antimicrobial.</description><subject>Actinobacillus Infections - microbiology</subject><subject>Actinobacillus Infections - veterinary</subject><subject>Actinobacillus pleuropneumoniae</subject><subject>Actinobacillus pleuropneumoniae - drug effects</subject><subject>Actinobacillus pleuropneumoniae - enzymology</subject><subject>Actinobacillus pleuropneumoniae - genetics</subject><subject>Actinobacillus pleuropneumoniae - isolation & purification</subject><subject>Animals</subject><subject>Anti-Infective Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Drug resistance</subject><subject>England</subject><subject>Genome, Bacterial</subject><subject>Hogs</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Sequence Data</subject><subject>Original Research</subject><subject>Pasteurellaceae</subject><subject>Pathogens</subject><subject>Plasmids</subject><subject>Sequence Analysis, DNA</subject><subject>Sulfisoxazole - pharmacology</subject><subject>Swine</subject><subject>Swine Diseases - microbiology</subject><subject>Tetrahydrofolate Dehydrogenase - genetics</subject><subject>Trimethoprim - pharmacology</subject><subject>Trimethoprim Resistance</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtrFEEUhQtRzCS68QdIgxsJtLn17toIQ_ARCLjRdVFdj6TG7qqxqjvBf28NE4O6cnUX9zuHe-5B6BWGdxgUvdgZe-G-32GCn6ANZgJ6Ago_RRugwHvJOD1Bp7XuAEBwMTxHJ4QrLulANuj-yvm0xBCtWWJOXQ6dC2WLWRdTt9znzsW6xGSXbj-ZOkdXO5tT8KXEdNMtJc5-uc37Nrvia2NNsv6g3domy6OxcZrW2tR-LXmf_DrnFI1_gZ4FM1X_8mGeoW8fP3y9_Nxff_l0dbm97i2jaunFEDg2IzAYiXMB-KgIsSMLQVAmJKjB8TAG54nAlGADWHiqOKODbWEtp2fo_dF3v46zd7aFLWbSh4NN-amzifrvTYq3-ibfacYBpJTN4O2DQck_Vl8XPcdq_TSZ5PNaNZaYcKEI-w9UKCGkEpI29M0_6C6vJbVPHCgJhBGARp0fKVtyrcWHx7sx6EP1ulWvj9U3-PWfSR_R313TXy27rTM</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Bossé, Janine T</creator><creator>Li, Yanwen</creator><creator>Walker, Stephanie</creator><creator>Atherton, Tom</creator><creator>Fernandez Crespo, Roberto</creator><creator>Williamson, Susanna M</creator><creator>Rogers, Jon</creator><creator>Chaudhuri, Roy R</creator><creator>Weinert, Lucy A</creator><creator>Oshota, Olusegun</creator><creator>Holden, Matt T G</creator><creator>Maskell, Duncan J</creator><creator>Tucker, Alexander W</creator><creator>Wren, Brendan W</creator><creator>Rycroft, Andrew N</creator><creator>Langford, Paul R</creator><general>Oxford Publishing Limited (England)</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20150801</creationdate><title>Identification of dfrA14 in two distinct plasmids conferring trimethoprim resistance in Actinobacillus pleuropneumoniae</title><author>Bossé, Janine T ; Li, Yanwen ; Walker, Stephanie ; Atherton, Tom ; Fernandez Crespo, Roberto ; Williamson, Susanna M ; Rogers, Jon ; Chaudhuri, Roy R ; Weinert, Lucy A ; Oshota, Olusegun ; Holden, Matt T G ; Maskell, Duncan J ; Tucker, Alexander W ; Wren, Brendan W ; Rycroft, Andrew N ; Langford, Paul R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-68f51ab040b2ddf05b922cb4ff63467098d5fbfde261321a016e395438c006c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Actinobacillus Infections - microbiology</topic><topic>Actinobacillus Infections - veterinary</topic><topic>Actinobacillus pleuropneumoniae</topic><topic>Actinobacillus pleuropneumoniae - drug effects</topic><topic>Actinobacillus pleuropneumoniae - enzymology</topic><topic>Actinobacillus pleuropneumoniae - genetics</topic><topic>Actinobacillus pleuropneumoniae - isolation & purification</topic><topic>Animals</topic><topic>Anti-Infective Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Drug resistance</topic><topic>England</topic><topic>Genome, Bacterial</topic><topic>Hogs</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Sequence Data</topic><topic>Original Research</topic><topic>Pasteurellaceae</topic><topic>Pathogens</topic><topic>Plasmids</topic><topic>Sequence Analysis, DNA</topic><topic>Sulfisoxazole - pharmacology</topic><topic>Swine</topic><topic>Swine Diseases - microbiology</topic><topic>Tetrahydrofolate Dehydrogenase - genetics</topic><topic>Trimethoprim - pharmacology</topic><topic>Trimethoprim Resistance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bossé, Janine T</creatorcontrib><creatorcontrib>Li, Yanwen</creatorcontrib><creatorcontrib>Walker, Stephanie</creatorcontrib><creatorcontrib>Atherton, Tom</creatorcontrib><creatorcontrib>Fernandez Crespo, Roberto</creatorcontrib><creatorcontrib>Williamson, Susanna M</creatorcontrib><creatorcontrib>Rogers, Jon</creatorcontrib><creatorcontrib>Chaudhuri, Roy R</creatorcontrib><creatorcontrib>Weinert, Lucy A</creatorcontrib><creatorcontrib>Oshota, Olusegun</creatorcontrib><creatorcontrib>Holden, Matt T G</creatorcontrib><creatorcontrib>Maskell, Duncan J</creatorcontrib><creatorcontrib>Tucker, Alexander W</creatorcontrib><creatorcontrib>Wren, Brendan W</creatorcontrib><creatorcontrib>Rycroft, Andrew N</creatorcontrib><creatorcontrib>Langford, Paul R</creatorcontrib><creatorcontrib>BRaDP1T Consortium</creatorcontrib><creatorcontrib>on behalf of the BRaDP1T Consortium</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bossé, Janine T</au><au>Li, Yanwen</au><au>Walker, Stephanie</au><au>Atherton, Tom</au><au>Fernandez Crespo, Roberto</au><au>Williamson, Susanna M</au><au>Rogers, Jon</au><au>Chaudhuri, Roy R</au><au>Weinert, Lucy A</au><au>Oshota, Olusegun</au><au>Holden, Matt T G</au><au>Maskell, Duncan J</au><au>Tucker, Alexander W</au><au>Wren, Brendan W</au><au>Rycroft, Andrew N</au><au>Langford, Paul R</au><aucorp>BRaDP1T Consortium</aucorp><aucorp>on behalf of the BRaDP1T Consortium</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of dfrA14 in two distinct plasmids conferring trimethoprim resistance in Actinobacillus pleuropneumoniae</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2015-08-01</date><risdate>2015</risdate><volume>70</volume><issue>8</issue><spage>2217</spage><epage>2222</epage><pages>2217-2222</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><abstract>The objective of this study was to determine the distribution and genetic basis of trimethoprim resistance in Actinobacillus pleuropneumoniae isolates from pigs in England.
Clinical isolates collected between 1998 and 2011 were tested for resistance to trimethoprim and sulphonamide. The genetic basis of trimethoprim resistance was determined by shotgun WGS analysis and the subsequent isolation and sequencing of plasmids.
A total of 16 (out of 106) A. pleuropneumoniae isolates were resistant to both trimethoprim (MIC >32 mg/L) and sulfisoxazole (MIC ≥256 mg/L), and a further 32 were resistant only to sulfisoxazole (MIC ≥256 mg/L). Genome sequence data for the trimethoprim-resistant isolates revealed the presence of the dfrA14 dihydrofolate reductase gene. The distribution of plasmid sequences in multiple contigs suggested the presence of two distinct dfrA14-containing plasmids in different isolates, which was confirmed by plasmid isolation and sequencing. Both plasmids encoded mobilization genes, the sulphonamide resistance gene sul2, as well as dfrA14 inserted into strA, a streptomycin-resistance-associated gene, although the gene order differed between the two plasmids. One of the plasmids further encoded the strB streptomycin-resistance-associated gene.
This is the first description of mobilizable plasmids conferring trimethoprim resistance in A. pleuropneumoniae and, to our knowledge, the first report of dfrA14 in any member of the Pasteurellaceae. The identification of dfrA14 conferring trimethoprim resistance in A. pleuropneumoniae isolates will facilitate PCR screens for resistance to this important antimicrobial.</abstract><cop>England</cop><pub>Oxford Publishing Limited (England)</pub><pmid>25957382</pmid><doi>10.1093/jac/dkv121</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-7453 |
| ispartof | Journal of antimicrobial chemotherapy, 2015-08, Vol.70 (8), p.2217-2222 |
| issn | 0305-7453 1460-2091 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4500777 |
| source | MEDLINE; Oxford University Press Journals Current; Full-Text Journals in Chemistry (Open access); EZB Free E-Journals; Alma/SFX Local Collection |
| subjects | Actinobacillus Infections - microbiology Actinobacillus Infections - veterinary Actinobacillus pleuropneumoniae Actinobacillus pleuropneumoniae - drug effects Actinobacillus pleuropneumoniae - enzymology Actinobacillus pleuropneumoniae - genetics Actinobacillus pleuropneumoniae - isolation & purification Animals Anti-Infective Agents - pharmacology Antibiotics Antimicrobial agents Drug resistance England Genome, Bacterial Hogs Microbial Sensitivity Tests Molecular Sequence Data Original Research Pasteurellaceae Pathogens Plasmids Sequence Analysis, DNA Sulfisoxazole - pharmacology Swine Swine Diseases - microbiology Tetrahydrofolate Dehydrogenase - genetics Trimethoprim - pharmacology Trimethoprim Resistance |
| title | Identification of dfrA14 in two distinct plasmids conferring trimethoprim resistance in Actinobacillus pleuropneumoniae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T19%3A23%3A09IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20dfrA14%20in%20two%20distinct%20plasmids%20conferring%20trimethoprim%20resistance%20in%20Actinobacillus%20pleuropneumoniae&rft.jtitle=Journal%20of%20antimicrobial%20chemotherapy&rft.au=Boss%C3%A9,%20Janine%20T&rft.aucorp=BRaDP1T%20Consortium&rft.date=2015-08-01&rft.volume=70&rft.issue=8&rft.spage=2217&rft.epage=2222&rft.pages=2217-2222&rft.issn=0305-7453&rft.eissn=1460-2091&rft_id=info:doi/10.1093/jac/dkv121&rft_dat=%3Cproquest_pubme%3E1696679673%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1697024200&rft_id=info:pmid/25957382&rfr_iscdi=true |