Characterization of a Bioflocculant (MBF-UFH) Produced by Bacillus sp. AEMREG7
A bioflocculant named MBF-UFH produced by a Bacillus species isolated from sediment samples of Algoa Bay of the Eastern Cape Province of South Africa was characterized. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG...
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| Veröffentlicht in: | International journal of molecular sciences 2015-06, Vol.16 (6), p.12986-13003 |
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| creator | Okaiyeto, Kunle Nwodo, Uchechukwu U Mabinya, Leonard V Okoli, Arinze S Okoh, Anthony I |
| description | A bioflocculant named MBF-UFH produced by a Bacillus species isolated from sediment samples of Algoa Bay of the Eastern Cape Province of South Africa was characterized. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG7 with Accession Number KP659187. The production of the bioflocculant was observed to be closely associated with cell growth. The bioflocculant had the highest flocculating activity of 83.2% after 72 h of cultivation, and approximately 1.6 g of purified MBF-UFH was recovered from 1 L of fermentation broth. Its chemical analyses indicated that it is a glycoprotein composed of polysaccharide (76%) and protein (14%). Fourier transform infrared spectroscopy (FTIR) revealed that it consisted of hydroxyl, amide, carboxyl and methoxyl as the functional moieties. Scanning electron microscopy (SEM) revealed the amorphous structure of MBF-UFH and flocculated kaolin clay particles. The maximum flocculating activity of 92.6% against kaolin clay suspension was achieved at 0.3 mg/mL over pH ranges of 3-11 with the peak flocculating rate at pH 8 in the presence of MgCl2. The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h. MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants. |
| doi_str_mv | 10.3390/ijms160612986 |
| format | Article |
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AEMREG7</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>PubMed Central</source><creator>Okaiyeto, Kunle ; Nwodo, Uchechukwu U ; Mabinya, Leonard V ; Okoli, Arinze S ; Okoh, Anthony I</creator><creatorcontrib>Okaiyeto, Kunle ; Nwodo, Uchechukwu U ; Mabinya, Leonard V ; Okoli, Arinze S ; Okoh, Anthony I</creatorcontrib><description>A bioflocculant named MBF-UFH produced by a Bacillus species isolated from sediment samples of Algoa Bay of the Eastern Cape Province of South Africa was characterized. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG7 with Accession Number KP659187. The production of the bioflocculant was observed to be closely associated with cell growth. The bioflocculant had the highest flocculating activity of 83.2% after 72 h of cultivation, and approximately 1.6 g of purified MBF-UFH was recovered from 1 L of fermentation broth. Its chemical analyses indicated that it is a glycoprotein composed of polysaccharide (76%) and protein (14%). Fourier transform infrared spectroscopy (FTIR) revealed that it consisted of hydroxyl, amide, carboxyl and methoxyl as the functional moieties. Scanning electron microscopy (SEM) revealed the amorphous structure of MBF-UFH and flocculated kaolin clay particles. The maximum flocculating activity of 92.6% against kaolin clay suspension was achieved at 0.3 mg/mL over pH ranges of 3-11 with the peak flocculating rate at pH 8 in the presence of MgCl2. The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h. MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms160612986</identifier><identifier>PMID: 26062133</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Bacillus ; Bacillus - chemistry ; Bacillus - genetics ; Bacteria ; Bacterial Proteins - biosynthesis ; Bacterial Proteins - chemistry ; Base Sequence ; Flocculation ; Glycoproteins - biosynthesis ; Glycoproteins - chemistry ; Kaolin - chemistry ; Molecular Sequence Data ; Polysaccharides, Bacterial - biosynthesis ; Polysaccharides, Bacterial - chemistry ; RNA, Ribosomal, 16S - genetics ; Sediments ; Spectrum analysis</subject><ispartof>International journal of molecular sciences, 2015-06, Vol.16 (6), p.12986-13003</ispartof><rights>Copyright MDPI AG 2015</rights><rights>2015 by the authors; licensee MDPI, Basel, Switzerland. 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-101d1535508e294ce97b99c94c088ca7a55a34e398dd61eb5c9fe0c553f7ece73</citedby><cites>FETCH-LOGICAL-c448t-101d1535508e294ce97b99c94c088ca7a55a34e398dd61eb5c9fe0c553f7ece73</cites><orcidid>0000-0002-9770-085X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490482/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490482/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26062133$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okaiyeto, Kunle</creatorcontrib><creatorcontrib>Nwodo, Uchechukwu U</creatorcontrib><creatorcontrib>Mabinya, Leonard V</creatorcontrib><creatorcontrib>Okoli, Arinze S</creatorcontrib><creatorcontrib>Okoh, Anthony I</creatorcontrib><title>Characterization of a Bioflocculant (MBF-UFH) Produced by Bacillus sp. AEMREG7</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>A bioflocculant named MBF-UFH produced by a Bacillus species isolated from sediment samples of Algoa Bay of the Eastern Cape Province of South Africa was characterized. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG7 with Accession Number KP659187. The production of the bioflocculant was observed to be closely associated with cell growth. The bioflocculant had the highest flocculating activity of 83.2% after 72 h of cultivation, and approximately 1.6 g of purified MBF-UFH was recovered from 1 L of fermentation broth. Its chemical analyses indicated that it is a glycoprotein composed of polysaccharide (76%) and protein (14%). Fourier transform infrared spectroscopy (FTIR) revealed that it consisted of hydroxyl, amide, carboxyl and methoxyl as the functional moieties. Scanning electron microscopy (SEM) revealed the amorphous structure of MBF-UFH and flocculated kaolin clay particles. The maximum flocculating activity of 92.6% against kaolin clay suspension was achieved at 0.3 mg/mL over pH ranges of 3-11 with the peak flocculating rate at pH 8 in the presence of MgCl2. The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h. MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.</description><subject>Bacillus</subject><subject>Bacillus - chemistry</subject><subject>Bacillus - genetics</subject><subject>Bacteria</subject><subject>Bacterial Proteins - biosynthesis</subject><subject>Bacterial Proteins - chemistry</subject><subject>Base Sequence</subject><subject>Flocculation</subject><subject>Glycoproteins - biosynthesis</subject><subject>Glycoproteins - chemistry</subject><subject>Kaolin - chemistry</subject><subject>Molecular Sequence Data</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>Polysaccharides, Bacterial - chemistry</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sediments</subject><subject>Spectrum analysis</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkctLAzEQxoMoPqpHrxLwooeteWxeF8GWVoX6QPQc0mxWU7abmuwK-te7xVrUk6cZmN98zDcfAIcY9SlV6MzP5glzxDFRkm-AXZwTkiHExeaPfgfspTRDiFDC1DbYId0CwZTugtvhi4nGNi76D9P4UMNQQgMHPpRVsLatTN3Ak5vBOHsaX53C-xiK1roCTt_hwFhfVW2CadGHF6Obh9Gl2AdbpamSO1jVHngajx6HV9nk7vJ6eDHJbJ7LJsMIF5hRxpB0ROXWKTFVynYdktIaYRgzNHdUyaLg2E2ZVaVDljFaCmedoD1w_qW7aKdzV1hXN9FUehH93MR3HYzXvye1f9HP4U3nuUK5JJ3AyUoghtfWpUbPfbKu6vy60CaNJRWUcPoflEuhlBQcdejxH3QW2lh3n9BYKCIpknx5fPZF2RhSiq5c342RXoaqf4Xa8Uc_za7p7xTpJ7XpmvM</recordid><startdate>20150608</startdate><enddate>20150608</enddate><creator>Okaiyeto, Kunle</creator><creator>Nwodo, Uchechukwu U</creator><creator>Mabinya, Leonard V</creator><creator>Okoli, Arinze S</creator><creator>Okoh, Anthony I</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7TK</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9770-085X</orcidid></search><sort><creationdate>20150608</creationdate><title>Characterization of a Bioflocculant (MBF-UFH) Produced by Bacillus sp. 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AEMREG7</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2015-06-08</date><risdate>2015</risdate><volume>16</volume><issue>6</issue><spage>12986</spage><epage>13003</epage><pages>12986-13003</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>A bioflocculant named MBF-UFH produced by a Bacillus species isolated from sediment samples of Algoa Bay of the Eastern Cape Province of South Africa was characterized. The bacterial identification was through 16S rDNA sequencing; nucleotide sequences were deposited in GenBank as Bacillus sp. AEMREG7 with Accession Number KP659187. The production of the bioflocculant was observed to be closely associated with cell growth. The bioflocculant had the highest flocculating activity of 83.2% after 72 h of cultivation, and approximately 1.6 g of purified MBF-UFH was recovered from 1 L of fermentation broth. Its chemical analyses indicated that it is a glycoprotein composed of polysaccharide (76%) and protein (14%). Fourier transform infrared spectroscopy (FTIR) revealed that it consisted of hydroxyl, amide, carboxyl and methoxyl as the functional moieties. Scanning electron microscopy (SEM) revealed the amorphous structure of MBF-UFH and flocculated kaolin clay particles. The maximum flocculating activity of 92.6% against kaolin clay suspension was achieved at 0.3 mg/mL over pH ranges of 3-11 with the peak flocculating rate at pH 8 in the presence of MgCl2. The bioflocculant retained high flocculating activity of 90% after heating at 100 °C for 1 h. MBF-UFH appears to have immense potential as an alternative to conventional chemical flocculants.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>26062133</pmid><doi>10.3390/ijms160612986</doi><tpages>18</tpages><orcidid>https://orcid.org/0000-0002-9770-085X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Bacillus Bacillus - chemistry Bacillus - genetics Bacteria Bacterial Proteins - biosynthesis Bacterial Proteins - chemistry Base Sequence Flocculation Glycoproteins - biosynthesis Glycoproteins - chemistry Kaolin - chemistry Molecular Sequence Data Polysaccharides, Bacterial - biosynthesis Polysaccharides, Bacterial - chemistry RNA, Ribosomal, 16S - genetics Sediments Spectrum analysis |
| title | Characterization of a Bioflocculant (MBF-UFH) Produced by Bacillus sp. AEMREG7 |
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