Study on a novel process for the separation of phospholipids, triacylglycerol and cholesterol from egg yolk
A novel process for effective separation of phospholipids, triacylglycerol and cholesterol from fresh egg yolk has been developed and validated in this study. Ethanol was the only organic solvent used in the whole procedure. Following initial separation of protein and total lipids by ethanol, most o...
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| Veröffentlicht in: | Journal of food science and technology 2015-07, Vol.52 (7), p.4586-4592 |
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| creator | Su, Yujie Tian, Ying Yan, Ruhui Wang, Chenying Niu, Fuge Yang, Yanjun |
| description | A novel process for effective separation of phospholipids, triacylglycerol and cholesterol from fresh egg yolk has been developed and validated in this study. Ethanol was the only organic solvent used in the whole procedure. Following initial separation of protein and total lipids by ethanol, most of solidified triacylglycerol was removed from total lipids by low temperature treatment of ethanol extracts within 10 h. Then, β-cyclodextrin (β-CD) was used to remove cholesterol from the remaining ethanol extracts and recycling of β-CD was also studied to obtain cholesterol and reusable β-CD powder. The highest cholesterol removal rate of nearly 99 % was obtained at β-CD: cholesterol molar ratio of 5:1, water addition of 15 g/g β-CD and reacting temperature of 50 °C. Ethanol in residual ethanol extracts was removed for obtaining phospholipids by rotary evaporation. The phospholipids produced in this procedure without cholesterol could be safety used as emulsifiers in food or cosmetic industry. |
| doi_str_mv | 10.1007/s13197-014-1513-5 |
| format | Article |
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Ethanol was the only organic solvent used in the whole procedure. Following initial separation of protein and total lipids by ethanol, most of solidified triacylglycerol was removed from total lipids by low temperature treatment of ethanol extracts within 10 h. Then, β-cyclodextrin (β-CD) was used to remove cholesterol from the remaining ethanol extracts and recycling of β-CD was also studied to obtain cholesterol and reusable β-CD powder. The highest cholesterol removal rate of nearly 99 % was obtained at β-CD: cholesterol molar ratio of 5:1, water addition of 15 g/g β-CD and reacting temperature of 50 °C. Ethanol in residual ethanol extracts was removed for obtaining phospholipids by rotary evaporation. The phospholipids produced in this procedure without cholesterol could be safety used as emulsifiers in food or cosmetic industry.</description><identifier>ISSN: 0022-1155</identifier><identifier>EISSN: 0975-8402</identifier><identifier>DOI: 10.1007/s13197-014-1513-5</identifier><identifier>PMID: 26139929</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Aqueous solutions ; beta-cyclodextrin ; Cardiovascular disease ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Cholesterol ; Crystallization ; egg yolk ; Eggs ; emulsifiers ; Ethanol ; Evaporation ; Extraction processes ; Fatty acids ; Food Science ; Hyperlipidemia ; industry ; Investigations ; Lipids ; Low temperature ; Nutrition ; Original ; Original Article ; phospholipids ; Phosphorus ; protein content ; Solvents ; Studies ; Temperature ; Toxicity ; triacylglycerols</subject><ispartof>Journal of food science and technology, 2015-07, Vol.52 (7), p.4586-4592</ispartof><rights>Association of Food Scientists & Technologists (India) 2014</rights><rights>Association of Food Scientists & Technologists (India) 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c709t-bc11e63eb91921bf4f6986a60afc2dfd55df41cccf005949f8a8b1e4830c6ffd3</citedby><cites>FETCH-LOGICAL-c709t-bc11e63eb91921bf4f6986a60afc2dfd55df41cccf005949f8a8b1e4830c6ffd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4486573/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4486573/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26139929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Su, Yujie</creatorcontrib><creatorcontrib>Tian, Ying</creatorcontrib><creatorcontrib>Yan, Ruhui</creatorcontrib><creatorcontrib>Wang, Chenying</creatorcontrib><creatorcontrib>Niu, Fuge</creatorcontrib><creatorcontrib>Yang, Yanjun</creatorcontrib><title>Study on a novel process for the separation of phospholipids, triacylglycerol and cholesterol from egg yolk</title><title>Journal of food science and technology</title><addtitle>J Food Sci Technol</addtitle><addtitle>J Food Sci Technol</addtitle><description>A novel process for effective separation of phospholipids, triacylglycerol and cholesterol from fresh egg yolk has been developed and validated in this study. Ethanol was the only organic solvent used in the whole procedure. Following initial separation of protein and total lipids by ethanol, most of solidified triacylglycerol was removed from total lipids by low temperature treatment of ethanol extracts within 10 h. Then, β-cyclodextrin (β-CD) was used to remove cholesterol from the remaining ethanol extracts and recycling of β-CD was also studied to obtain cholesterol and reusable β-CD powder. The highest cholesterol removal rate of nearly 99 % was obtained at β-CD: cholesterol molar ratio of 5:1, water addition of 15 g/g β-CD and reacting temperature of 50 °C. Ethanol in residual ethanol extracts was removed for obtaining phospholipids by rotary evaporation. The phospholipids produced in this procedure without cholesterol could be safety used as emulsifiers in food or cosmetic industry.</description><subject>Aqueous solutions</subject><subject>beta-cyclodextrin</subject><subject>Cardiovascular disease</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Cholesterol</subject><subject>Crystallization</subject><subject>egg yolk</subject><subject>Eggs</subject><subject>emulsifiers</subject><subject>Ethanol</subject><subject>Evaporation</subject><subject>Extraction processes</subject><subject>Fatty acids</subject><subject>Food Science</subject><subject>Hyperlipidemia</subject><subject>industry</subject><subject>Investigations</subject><subject>Lipids</subject><subject>Low temperature</subject><subject>Nutrition</subject><subject>Original</subject><subject>Original Article</subject><subject>phospholipids</subject><subject>Phosphorus</subject><subject>protein content</subject><subject>Solvents</subject><subject>Studies</subject><subject>Temperature</subject><subject>Toxicity</subject><subject>triacylglycerols</subject><issn>0022-1155</issn><issn>0975-8402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqFkV9r1jAUxoM43Jj7AN5IwBsvrOYkTdvcCDLmHxh4se06pOlJ3255m5q0g357U985piAGQhLO7zwn5zyEvAL2HhirPyQQoOqCQVmABFHIZ-SEqVoWTcn483xnnBcAUh6Ts5RuWV6C1w1nL8gxr0AoxdUJubual26lYaSGjuEePZ1isJgSdSHSeYc04WSimYeMBEenXUh5-2EauvSOznEwdvW9Xy3G4KkZO2pzGNP86-1i2FPse7oGf_eSHDnjE549nKfk5vPF9fnX4vL7l2_nny4LWzM1F60FwEpgq0BxaF3pKtVUpmLGWd65TsrOlWCtdYxJVSrXmKYFLBvBbOVcJ07Jx4PutLR77CyOczReT3HYm7jqYAb9Z2QcdroP97osm0rWIgu8fRCI4ceSe9H7IVn03owYlqT5NksGCvh_UaiUqHMfckPf_IXehiWOeRIbxblQjdxqw4GyMaQU0T3-G5jejNcH43U2Xm_Ga5lzXj9t-DHjt80Z4Acg5dDYY3xS-p-qPwE3iLq2</recordid><startdate>20150701</startdate><enddate>20150701</enddate><creator>Su, Yujie</creator><creator>Tian, 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Technol</addtitle><date>2015-07-01</date><risdate>2015</risdate><volume>52</volume><issue>7</issue><spage>4586</spage><epage>4592</epage><pages>4586-4592</pages><issn>0022-1155</issn><eissn>0975-8402</eissn><abstract>A novel process for effective separation of phospholipids, triacylglycerol and cholesterol from fresh egg yolk has been developed and validated in this study. Ethanol was the only organic solvent used in the whole procedure. Following initial separation of protein and total lipids by ethanol, most of solidified triacylglycerol was removed from total lipids by low temperature treatment of ethanol extracts within 10 h. Then, β-cyclodextrin (β-CD) was used to remove cholesterol from the remaining ethanol extracts and recycling of β-CD was also studied to obtain cholesterol and reusable β-CD powder. The highest cholesterol removal rate of nearly 99 % was obtained at β-CD: cholesterol molar ratio of 5:1, water addition of 15 g/g β-CD and reacting temperature of 50 °C. Ethanol in residual ethanol extracts was removed for obtaining phospholipids by rotary evaporation. The phospholipids produced in this procedure without cholesterol could be safety used as emulsifiers in food or cosmetic industry.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>26139929</pmid><doi>10.1007/s13197-014-1513-5</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aqueous solutions beta-cyclodextrin Cardiovascular disease Chemistry Chemistry and Materials Science Chemistry/Food Science Cholesterol Crystallization egg yolk Eggs emulsifiers Ethanol Evaporation Extraction processes Fatty acids Food Science Hyperlipidemia industry Investigations Lipids Low temperature Nutrition Original Original Article phospholipids Phosphorus protein content Solvents Studies Temperature Toxicity triacylglycerols |
| title | Study on a novel process for the separation of phospholipids, triacylglycerol and cholesterol from egg yolk |
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